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Journal of Integrative Plant Biology 2009年第3期51卷 287-298页

作者： Fan lin yun zhang Ming-Yi Jiang College of Life Sciences Nanjing Agricultural University Nanjing 210095 China

With the exception of rice, little is known about the existence of respiratory burst oxidase homolog （rboh） gene in cereals. The present study reports the cloning and analysis of a novel rboh gene, termed ZmrbohB, from maize （Zea mays L.）. The full-length cDNA of ZmrbohB encodes a 942 amino acid protein containing all of the respiratory burst oxidase homolog catalytically critical motifs. Alternative splicing of ZmrbohB has generated two transcript isoforms, ZmrbohB-α and -β. Spliced transcript ZmrbohB-β retains an unspliced intron 11 that carries a premature termination codon and probably leads to nonsense-mediated mRNA decay. Expression analysis showed that two splice isoforms were differentially expressed in various tissues and at different developmental stages, and the major product was ZmrbohB-e. The transcripts of ZmrbohB-α accumulated markedly when the maize seedlings were subjected to various abiotic stimuli, such as wounding, cold （4℃）, heat （40℃）, UV and salinity stress. In addition, several abiotic stimuli also affected the alternative splicing pattern of ZmrbohB except wounding. These results provide new insight into roles in the expression regulation of plant rboh genes and suggest that ZrnrbohB gene may play a role in response to environmental stresses.

关键词： abiotic stress alternative splicing respiratory burst oxidase homolog Zea mays.

Enigmatic rapid organization of subdural hematoma in a patient with epilepsy:A case report

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World Journal of Clinical Cases 2022年第13期10卷 4288-4293页

作者： Hong-Tao Lv lin-yun zhang Xiao-Tong Wang Department of Neurosurgery The First Affiliated Hospital of Dalian Medical UniversityDalian 116000Liaoning ProvinceChina

BACKGROUND Determining a subdural hematoma(SDH)to be chronic by definition takes 3 wk,whereas organized chronic SDH(OCSDH)is an unusual condition that is believed to form over a much longer period of time,which generally demands large ***,it is a lengthy process from the initial head trauma,if any,to the formation of an *** SDH(ASDH)with organization-like,membranaceous appearances has never been *** SUMMARY A 56-year-old woman presented to our hospital with a seizure,and computed tomography(CT)on admission was negative for signs of intracranial *** had clear consciousness and unimpaired motor functions on arrival and remained stable for the following week,during which she underwent necessary *** the morning of day 10 of hospitalization,she accidentally hit her head hard against the wall in the bathroom and promptly lapsed into complete coma within 2 ***,we performed emergency CT and identified a left supratentorial SDH that was an absolute indication for ***,the intraoperative findings were surprising,with no liquefaction ***,a solid hematoma covered with a thick membrane was noted that strongly resembled an organized *** was successful,but the family stopped treatment the next day due to financial problems,and the patient soon *** Neurosurgeons should address SDHs,especially ASDHs,with discretion and individualization due to their highly diversified features.

关键词： Organized chronic subdural hematoma Acute subdural hematoma Subacute subdural hematoma Craniotomy Encapsulation Case report

Creating rat hepatocyte organoid as an in vitro model for drug testing

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World Journal of Stem Cells 2020年第10期12卷 1184-1195页

作者： Yu-Ting He Xing-Long Zhu Sheng-Fu Li Bing-Qi zhang Yi Li Qiong Wu yun-lin zhang Yan-Yan Zhou Li Li Ya-Na Qi Ji Bao Hong Bu Laboratory of Pathology Key Laboratory of Transplant Engineering and ImmunologyNHCWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Key Laboratory of Transplant Engineering and Immunology NHCWest China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Chinese Evidence-based Medicine Center West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Pathology West China HospitalChengdu 610041Sichuan ProvinceChina

BACKGROUND Liver organoids have recently been applied as models for liver disease and drug screening,especially when combined with liver-on-a-chip *** to hepatocyte-like cells,primary hepatocytes have high functionality but cannot maintain their function when cultured in *** stem cells(MSCs)enhance hepatocyte function and maintain hepatocyte metabolism when co-cultured with *** can help induced pluripotent stem cells to generate an organoid structure via the MSC-based traction force triggered by extracellular matrix(ECM)*** this study,primary hepatocytes were cocultured with MSCs on a liver-derived ECM to generate liver organoids within a short *** To create hepatocyte organoids by co-culturing primary hepatocytes with MSCs on a porcine liver extracellular matrix(PLECM)*** Perfusion and enzymatic hydrolysis were used to form the PLECM *** hepatocytes and human MSCs were mixed and plated on pre-solidified PLECM gel in a 48-well plate for 48 h to generate *** organoids were evaluated through hematoxylin and eosin,periodic acid-Schiff,immunohistological,and immunofluorescence staining,and quantitative PCR for alb,CYP450 gene markers,and urea cycle *** medium was collected to detect albumin(ALB)and urea production on days 2,4,6,8,14,and *** The whole porcine liver was perfused and enzymatically hydrolyzed to form a PLECM *** structural components and basement membrane composition of the ECM,such as collagen type I,collagen type IV,fibronectin,and laminin,were demonstrated to be *** interaction of human MSCs with the liverderived ECM,primary hepatocytes and human MSCs assembled together into a 3D construction and generated primary hepatocyte organoids for 48 *** mRNAs of the gene alb,the CYP450 gene markers cyp1a1,cyp1a2,and cyp3a2 as well as urea cycle genes arg-1,asl,ass-1,cps-1,nags were highly expressed in hepatocyte ***-term surviv

关键词： Organoid Primary hepatocytes Stem cells Liver therapies Extracellular matrix Drug screening

基于村域的乡村多功能类型划分及评价研究——以江苏省金坛市为例

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长江流域资源与环境 2017年第3期26卷 359-367页

作者：李智范琳芸张小林南京师范大学地理科学学院江苏南京210023 江苏省地理信息资源开发与利用协同创新中心江苏南京210023

随着城镇化的快速推进,我国经济社会发展进入转型期,乡村地域向多元方向发展和演变。近年来,乡村多功能发展成为地理学研究的重要课题,成为指导乡村转型发展的新理论和新范式。在系统梳理乡村多功能发展概念及其内涵基础上,探索村域尺... 详细信息

随着城镇化的快速推进,我国经济社会发展进入转型期,乡村地域向多元方向发展和演变。近年来,乡村多功能发展成为地理学研究的重要课题,成为指导乡村转型发展的新理论和新范式。在系统梳理乡村多功能发展概念及其内涵基础上,探索村域尺度下我国乡村地域多功能的类型划分及其评价方法,并开展了江苏省金坛市乡村地域生态功能、农业功能、工业功能、生活功能及其综合功能的分级评价与空间分异研究,进而识别出全市分村域的地域主导功能类型。结果表明:生态功能高值区主要分布在环长荡湖、直溪镇和薛埠镇等水网密集、林地资源丰富的地区;农业功能高值区主要分布在金坛市中部和南部等河网平原地区;工业功能高值区主要集中在中心城区周边和尧塘镇等城市集聚发展区域;生活功能高值区主要集中在中心城区周边等地;乡村地域多功能指数呈现出以城区为中心、向外围逐渐递减的空间分异特征;全市乡村地域主导功能可划分为6种类型,并揭示其空间分布特征、分别总结其所对应的乡村发展策略。研究结果可为明确乡村功能定位、优化主体功能区划、完善乡村管理政策以及促进乡村可持续发展提供科学依据。

关键词：村域多功能类型划分评价金坛市

CSI理论框架下农户农地转出满意度影响因素分析——以武汉城市圈典型地区调查为例

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中国土地科学 2017年第2期31卷 67-76页

作者：陈璐甘臣林梅昀张苗华中农业大学公共管理学院湖北武汉430070

研究目的:探索农地转出中农户满意度的形成过程与机理,从农户主观感受视角来定量分析农地流转政策的制约因素。研究方法:基于顾客满意度指数(Customer Satisfaction Index,CSI)理论框架,构建农户农地流转满意度指数(Farmer Satisfaction... 详细信息

研究目的:探索农地转出中农户满意度的形成过程与机理,从农户主观感受视角来定量分析农地流转政策的制约因素。研究方法:基于顾客满意度指数(Customer Satisfaction Index,CSI)理论框架,构建农户农地流转满意度指数(Farmer Satisfaction Index,FSI)模型,运用结构方程模型(Structural Equation Model,SEM)进行定量分析。研究结果:农户对农地转出的感知质量是影响其满意度的决定因素,可靠性是关键因素,政府形象与印象感知是重要因素,人员互动是有效因素。研究结论:要加强农村社会保障制度建设以确保农民获得公平的社会保障,规范流转程序、健全流转监督机制、保护农民利益,加强政府廉政建设以改善政府形象以提高农户满意度,促进农地转出。

关键词：土地经济农地流转 CSI 农户满意度结构方程模型武汉城市圈

Transfer RNA-derived small RNA serves as potential non-invasive diagnostic marker and a novel therapeutic target for acute pancreatitis

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World Journal of Gastroenterology 2025年第1期31卷 152-156页

作者： Jing zhang Chun-lin Ou Department of Immunology School of Basic MedicineCentral South UniversityChangsha 410000Hunan ProvinceChina Department of Pathology Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina

Transfer RNA(tRNA)-derived fragments,a new type of tRNA-derived small RNA(tsRNA),can be cleaved from tRNA by enzymes to regulate target gene expression at the transcriptional and translational *** are not only degradation fragments but also have biological functions,including those in immune inflammation,metabolic disorders,and cell *** dysregulation is closely associated with multiple diseases,including various cancers and acute pancreatitis(AP).AP is a common gastrointestinal disease,and its incidence increases *** development is associated with tsRNAs,which regulate cell injury and induce inflammation,especially pyroptosis and ***,serum tRF36 has the potential to serve as a non-invasive diagnostic biomarker and leads to pancreatic acinar cell ferroptosis causing inflammation to promote *** show the characteristics of tsRNAs and their diagnostic value and function in AP,and discuss the potential opportunities and challenges of using tsRNAs in clinical applications and research.

关键词： Transfer RNA derived small RNAs Acute pancreatitis Liquid biopsy Biomarker Cell pyroptosis Ferroptosis

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日本的环境政策及其对我国的启示

生产力研究 2006年第10期 166-168,270页

作者：时临云张宏武天津商学院天津300134

日本在二战以后曾经历过环境污染加剧的过程,环境问题从产业公害型演变为城市生活型。但由于采取了有效、可行的环境政策,目前已成为世界上环境质量较好的国家之一。文章通过对战后日本环境问题的变迁的回顾和分析,对借鉴日本解决环境... 详细信息

日本在二战以后曾经历过环境污染加剧的过程,环境问题从产业公害型演变为城市生活型。但由于采取了有效、可行的环境政策,目前已成为世界上环境质量较好的国家之一。文章通过对战后日本环境问题的变迁的回顾和分析,对借鉴日本解决环境问题的法规、政策、方法和经验,构建我国的环境政策体系,提高全民的环境意识,加强环境经济学的理论研究和人才培养等提出了认识和看法。

关键词：日本环境政策启示公害问题循环型社会

Surfactant Replacement Therapy for the Prevention of the Neonatal Respiratory Distress Syndrome in Preterm Infants

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中国当代儿科杂志 2001年第4期 482-486页

作者： ZHOU Xiao-Guang, LUO Xian-Qiong, YANG lin-lin, CHEN yun-Bin, zhang Xiao-Zhuang, ZHAO Qing-GuoDepartment of Neonatology, Maternal and Child Health Hospital of Guangdong Province, Guangzhou 510010,China

Objective To study the efficacy of pulmonary surfactant (Exosurf) in the prevention of the neonatal respiratory distress syndrome (RDS) in preterm infants. Methods A prospective clinical trial was conducted. To prevent RDS, a single dose of pulmonary surfactant (Exosurf) was administered intratracheally in 25 preterm infants at a high risk of developing RDS as the prophylaxis group, and another 25 preterm babies who received no surfactant administration formed the control group. Results The preterm infants in the prophylaxis group received a prophylactic dose of surfactant ( 67.5 mg/kg) within 0.25 to 6 hours ( 3.4± 1.9 hours) after delivery. Oxygenation in these babies was markedly improved and their clinical symptoms were relieved after the administration of surfactant. The durations of supplemental oxygen administration, assisted ventilation and hospitalization in the prophylaxis group, were ( 9.5± 6.9) days, ( 2.6± 3.8) days and ( 40.8± 17.8) days respectively, which were significantly shortened compared with those of the control group (P 0.05). Conclusions Prophylactic administration of surfactant can improve oxygenation, relieve symptoms, and shorten the duration of supplemental oxygen administration, assisted ventilation and hospitalization. It has a relief effect on RDS in preterm infants.

关键词： Prematurity Respiratory distress syndrome Pulmonary surfactant Prophylactic administration

Dielectric-ion-conductive ZnNb2O6 layer enabling rapid desolvation and diffusion for dendrite-free Zn metal batteries

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Journal of Energy Chemistry 2025年第1期100卷 693-701页

作者： Haifeng Yang Jian Wang Panpan zhang Xiaomin Cheng Qinghua Guan Jing Dong Bixian Chen Lujie Jia Jing zhang Yongzheng zhang yunjian Liu Hongzhen lin School of Materials Science and Engineering Jiangsu University i-Lab & CAS Key Laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences Helmholtz Institute Ulm (HIU) Karlsruhe Institute of Technology (KIT) School of Materials Science and Engineering Xi'an University of Technology State Key Laboratory of Chemical Engineering East China University of Science and Technology

Rechargeable aqueous zinc-metal batteries （AZMBs） are promising candidates for large-scale energy storage systems due to their low cost and high ***,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn（H2O）62+solvation ***,learnt from self-generated solid electrolyte interphase （SEI） in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface （ZNB@Zn）,acting as a rapid desolvation *** zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical ***,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility （99.54%） even under high current density and depth of discharge ***,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g-1and outstanding rate performance up to 10 A *** large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.

关键词： Zn metal battery Dielectric artificial layer Rapid ion diffusion Zincophilic diffusion Active water inhibition

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利用贪心近似算法的人脸识别方法

控制工程 2017年第10期24卷 2125-2129页

作者：林云峰张莉华丽水学院工学院浙江丽水323000 黄淮学院信息工程学院河南驻马店463000

针对网络在线人脸识别方法候选标签较多和识别精度较低的问题,提出一种基于贪心近似算法(Greedy Approximation Algorithm,GAA)结合结构化支持向量机(Structural Support Vector Machine,SSVM)的人脸识别方法。该方法的主要思想是相册... 详细信息

针对网络在线人脸识别方法候选标签较多和识别精度较低的问题,提出一种基于贪心近似算法(Greedy Approximation Algorithm,GAA)结合结构化支持向量机(Structural Support Vector Machine,SSVM)的人脸识别方法。该方法的主要思想是相册结构的人脸图像反应了标签间的兼容关系,利用标签成本可以控制相册中标签的数量。首先按照标签成本对相册中的个人进行建模,给出建模过程中解决NP问题的贪心近似算法。然后,模型参数通过SSVM训练获得。最后,利用最大隶属度原则完成人脸识别。利用从Facebook获取的2个独立图像数据库进行对比实验,实验结果表明,该方法明显提高了人脸识别率,高于其他优秀方法 10%左右。此外,该方法还能包含更多其他有用信息,具有较好的兼容性。

关键词：人脸识别标签成本贪心近似算法结构化支持向量机最大隶属度原则