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Preface to Special Issue on Novel Semiconductor-biochemical Sensors

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Journal of Semiconductors 2023年第2期44卷 9-10页

作者： Zhao Li xiangmei lin Dongxian He Yingxin Ma Yuanjing lin State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Beijing Key Laboratory of Genome and Precision Medicine Technologies Beijing 100101China Chinese Academy of Inspection and Quarantine Beijing 100123China College of Water Resources and Civil Engineering China Agricultural UniversityBeijing 100083China CAS Key Laboratory of Quantitative Engineering Biology Guangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China School of Microelectronics Southern University of Science and TechnologyShenzhen 518033China

Since 2020,the global outbreak and continued evolution of the COVID-19 pandemic have brought the concepts of nucleic acid,antigen-antibody,gene sequencing,and other biochemical testing into ordinary *** this regard,novel semiconductor-biochemical sensors that convert biochemical information into monitorable electrical and optical signals according to specific rules have become increasingly important and *** sensors deeply fuse the technical advantages of semiconductors and biochemistry,integrating interdisciplinary subjects such as molecular biology,nanomaterials,microfluidics,artificial intelligence(AI),*** the advantages of fast speed,high sensitivity,high integration,easy mass manufacturing,the novel technologies are the"pioneer"of biomedical information acquisition and the"heart"of modern medical diagnostic ***,the technologies are showing a spurt of development,with new products emerging,new functions being developed,and new application scenarios being *** research hotspots cover a wide range,including immediate detection,non-invasive analysis,wearable devices,on-site monitoring,*** issue looks at the latest advances in the novel technologies for physiological dynamic monitoring of animals/plants and rapid detection of highly pathogenic pathogens,while covering applications in agriculture,fisheries,animal husbandry,biosecurity,and wearable medicine.

关键词： husbandry integrating expanded

A brief review of novel nucleic acid test biosensors and their application prospects for salmonids viral diseases detection

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Journal of Semiconductors 2023年第2期44卷 49-56页

作者： Xiaofei Liu Songyin Qiu Haiping Fang lin Mei Hongli Jing Chunyan Feng Shaoqiang Wu xiangmei lin Institute of Animal Inspection and Quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Research Center of GACC for international Inspection and Quarantine Standards and Technical Regulations Beijing 100013China

Viral diseases represent one of the major threats for salmonids *** detection and identification of viral pathogens is the main prerequisite prior to undertaking effective prevention and control ***,sensitive,efficient and portable detection method is highly essential for fish viral diseases *** strategies are highly prevalent and fulfill the expanding demands of on-site detection with fast response,cost-effectiveness,high sensitivity,and *** the development of material science,the nucleic acid biosensors fabricated by semiconductor have shown great potential in rapid and early detection or screening for diseases at salmonids *** paper reviews the current detection development of salmonids viral *** present limitations and challenges of salmonids virus diseases surveillance and early detection are *** nucleic acid semiconductor biosensors are briefly *** perspective and potential application of biosensors in the on-site detection of salmonids diseases are discussed.

关键词： salmonids virus detection nucleic acid test biosensors semiconductor

Identification of tick-borne pathogens using metagenomic analyses in *** feeding on humans in downtown Beijing

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Animal Diseases 2021年第3期1卷 173-181页

作者： Jizhou Lv Huiyu Wang Xueqing Han lin Mei Xiangfen Yuan Yufang Kong Junhua Deng Zhen F.Fu Shaoqiang Wu xiangmei lin Institute of Animal Inspection and Quarantine Chinese Academy of Inspection and QuarantineBeijing 100176China Department of Pathology College of Veterinary MedicineUniversity of GeorgiaAthens 30602USA

On August 14th,2018,a Beijing resident living in Xicheng District found a female *** tick attached to the skin at the front of his upper *** examination,the patient was afebrile and appeared *** species of the tick was identified through morphological characteristics and phylogenetic analysis based on cytochrome C oxidase subunit *** *** tick was screened for tick-borne pathogens such as viruses,bacteria and *** pathogens were screened by PCR and sequencing,while DNA pathogens were screened by metagenomic *** was found that the tick was positive for the DNA sequences of zoonotic and animal pathogens such as A phagocytophilum,Ehrlichia minasensis and *** the good health condition of the patient,we hypothesized that the pathogens originated from the tick specimen itself rather than host blood *** the first time,our study reveals the possible risk of transmission of tick-borne pathogens to human beings through tick bit in downtown *** research is needed to screen for tick-borne pathogens among unfed ticks collected from central Beijing.

关键词： Tick-borne pathogens Metagenomic analyses H.longicornis Downtown Beijing

Prevalence and Countermeasures of Porcine Circovirus 3

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Animal Husbandry and Feed Science 2018年第3期10卷 185-188页

作者： Feng Chunyan Wang Caixia Liu Dandan Zhang Yongning Du Fangyuan lin xiangmei Wu Shaoqiang Animal Quarantine Institute Chinese Academy of Inspection and Quarantine

The development history, etiology, epidemiology, clinical symptoms, pathological changes and diagnosis technology of porcine circovirus 3 were summarized, and countermeasures for prevention and control of the disease ... 详细信息

关键词： Porcine circovirus 3 Etiology Epidemiology Diagnosis Prevention and control

Antigen-capture ELISA for grass carp reovirus 873

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Antigen-capture ELISA for grass carp reovirus 873

Molecular Identification of Hyalomma anatolicum,Hyalomma asiaticum and Hyalomma detritum

中国水产学会鱼病专业委员会2013年学术研讨会

作者： Jing Hongli Gao long ying Zhang Min Wang Na lin xiangmei Wu shaoqiang Research Centre of Aquatic Animal Diseases the Institute of Animal QuarantineChinese Academy of Inspection and Quarantine Shen zhen Entry-exit Inspection and Quarantine Bureau

An antigen-capture ELISA for grass carp reovirus 873(GCRV 873)was *** employs polyclonal antiserum directed against grass carp reovirus *** antigen boundby the capture mAb(16B7)was detected by addition of the goat anti-mouse Ig peroxidaseconjugation,followed by addition of the enzyme ***-873 was detected in culturesupernatants(6.25×105pfu/ml)and in ectracts of kidney and spleen of infected grass *** was highly specific:Viral hemorrhagic septicemia virus,Infectious haematopoietic necrosisvirus,spring viraemia of carp virus,Epizootic haematopoietic necrosis virus,Chinook salmonreovirus could not be detected by the antigen-capture ELISA for GCRV 873.

关键词： Grass carp reovirus(GCRV) fish viral diseases Antigen-capture ELISA

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Animal Husbandry and Feed Science 2014年第3期6卷 118-122页

作者： Lv Jizhou Wu Shaoqiang Zhang Yongning Wang Zhenbao Feng Chunyan Wang Caixia Deng Junhua Yuan Xiangfen lin xiangmei Institute of Animal Quarantine Chinese Academy of Inspection and Quarantine Yili Entry-exit Inspection and Quarantine Bureau

Hyalomma anatolicum, Hyalomma asiaticum and Hyalomma detritum are wide-spread tick vectors in China. They could transmit a great variety of serious animal and human pathogens, which are great threats to the health of human beings and the safety of stockbreeding. Most of them are distributed in Xinjiang and Inner Mongolia, and they share similar morphologies. This study is to establish a method for identifying H. anatolicum, H. asiaticum and H. detritum with molecular markers and to revealing the phylogenetic relationship of these ticks. Ticks were collected from domestic animals in Xinjiang and Inner Mongolia autonomous regions and classified by morphological characters. 16S rRNA and mitachondrial cytochrome oxidase subunit I gone （CO1） of ticks were amplified by PCR and subsequently sequenced. Phylogenetic trees were constructed by MEGA 5.0 and Mrbayes 3.2. On the 16S rRNA-based phylogenetic tree, H. anatolicum and H. aisaticum were clustered together with their respective classes. H. detritum was clustered with their respective class and the H. anatolicum, H. asiaticum formed distinct branches on the phylogenetic trees based on the COL The method based on morphology that combined with molecular 16S rRNA and COl seemed a simple and accurate method for species identification of H. anatolicum, H. asiaticum and H. detritum. n

关键词： Cytochrome oxidase subunit I gone (COI) 16S rRNA Hyalomma anatolicum Hyalomma asiaticum Hyalomma detritum Molecular identificatio

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蛙虹彩病毒巢式PCR检测方法的建立

中国水产科学 2011年第3期18卷 661-666页

作者：张旻林祥梅江育林中国检验检疫科学研究院动植物检疫研究所北京100029

蛙虹彩病毒属(Ranavirus)病毒宿主广泛,可以感染爬行类、鱼类和两栖类,大部分病毒对宿主都有较强的致病性和致死性。为建立一种快速高效的蛙虹彩病毒的检测方法,本研究利用中华鳖虹彩病毒(Soft-shelled Turtle Iridovirus,STIV)核衣壳蛋... 详细信息

蛙虹彩病毒属(Ranavirus)病毒宿主广泛,可以感染爬行类、鱼类和两栖类,大部分病毒对宿主都有较强的致病性和致死性。为建立一种快速高效的蛙虹彩病毒的检测方法,本研究利用中华鳖虹彩病毒(Soft-shelled Turtle Iridovirus,STIV)核衣壳蛋白(Major Capsid Protein,MCP)基因保守区设计内引物和外引物,建立了特异性检测流行性造血器官坏死病毒(Epizootic Haematopoietic Necrosis Virus,EHNV)、中华鳖虹彩病毒和虎纹蛙虹彩病毒(Tiger Frog Virus,TFV)的巢式PCR(巢式PCR)检测方法,并制备了重组质粒pGem-T-S作为阳性对照标准品。检测限试验结果显示,该方法可以检测102拷贝的病毒粒子。而且与传染性造血器官坏死病毒、鲤春病毒、病毒性出血性败血症病毒、斑点叉尾病毒、传染性胰脏坏死病毒、真鲷虹彩病毒、牙鲆弹状病毒以及锦鲤疱疹病毒等其他非蛙虹彩病毒无交叉反应。该体系具有简便、快速、敏感、特异性高、低成本等特点,为诊断与预防蛙虹彩病毒提供了一项重要的技术手段。

关键词：蛙虹彩病毒中华鳖虹彩病毒流行性造血器官坏死病毒虎纹蛙虹彩病毒巢式PCR

指向科学素养的初中地理跨学科主题研学设计——以“走进温泉·探研地热能”为例

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地理教学 2024年第21期 55-58页

作者：林香妹真丽倩林闽峰福清市教师进修学校福建福州350300 福建师范大学附属福清德旺中学福建福州350319

地理教育是科学教育的组成部分。在真实情景中,教师组织开展以地理学科为中心的跨学科主题研学旅行活动,有助于引导学生系统地进行科学探究实践,经历“认知—实践—深化认知”的过程,提升科学素养。本文从研学旅行的课程性质与科学学科... 详细信息

地理教育是科学教育的组成部分。在真实情景中,教师组织开展以地理学科为中心的跨学科主题研学旅行活动,有助于引导学生系统地进行科学探究实践,经历“认知—实践—深化认知”的过程,提升科学素养。本文从研学旅行的课程性质与科学学科核心素养之间的内在联系出发,构建“问题解决或主题深入学习”的跨学科主题研学设计框架,以“走进温泉·探研地热能”为例,探索科学素养的培育路径。

关键词：科学素养跨学科研学旅行初中地理

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高通量测序分析5例进口鱼粉鱼源性成分构成

中国畜牧兽医 2021年第1期48卷 93-100页

作者：袁向芬吴绍强林祥梅吕继洲中国检验检疫科学研究院北京100176

为揭示鱼粉原料构成,保证鱼粉质量,本研究应用高通量测序技术对来源于不同国家的5例进口鱼粉样品进行测序,并基于COI基因及其丰度对鱼粉鱼源性成分构成进行分析。提取鱼粉样品DNA后应用鱼类通用COI基因扩增引物进行PCR扩增,得到鱼类混合... 详细信息

为揭示鱼粉原料构成,保证鱼粉质量,本研究应用高通量测序技术对来源于不同国家的5例进口鱼粉样品进行测序,并基于COI基因及其丰度对鱼粉鱼源性成分构成进行分析。提取鱼粉样品DNA后应用鱼类通用COI基因扩增引物进行PCR扩增,得到鱼类混合COI基因序列产物,将扩增产物送测序公司进行高通量测序,将COI基因测序数据与鱼类相关数据库进行比对,通过比对结果进行鱼粉的物种源性成分分析。结果显示,成功获得了5例鱼粉样品中原料鱼成分,其中白鱼粉原料鱼主要成分为鳕鱼;而红鱼粉原料鱼成分较为多样,涉及鲱鱼、竹刀鱼等。通过Python对数据进行统计分析,绘制PCR扩增后鱼类COI基因丰度图,进一步直观提示鱼粉样品中原料的成分构成。本研究结果表明,高通量测序技术可应用于鱼粉质量评估,有助于快速了解鱼粉构成,对其质量及用途进行指导,同时,提示该技术可进一步应用于鱼粉掺杂掺假及致病微生物的监测工作中,对保障鱼粉产品质量、维护正常市场秩序具有重要意义。

关键词：高通量测序鱼粉成分分析 COI基因丰度