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The dynamic changes of genes revealed that persistently overexpressed genes drive the evolution of cyflumetofen resistance in Tetranychus cinnabarinus

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Insect Science 2023年第4期30卷 1129-1148页

作者： Kaiyang Feng Jialu Liu Mingyu Zhao Zhixin Jiang peilin Liu Peng Wei Wei Dou lin He Key Laboratory of Entomology and Pest Control Engineering College of Plant ProtectionSouthwest UniversityChongqingChina Academy of Agricultural Sciences Southwest UniversityChongqingChina State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University Southwest UniversityChongqingChina Key Scientific Research Base of Pest and Mold Control of Heritage Collection(Chongqing China Three Gorges Museum) State Administration of Cultural HeritageChongqingChina

Changes in gene expression are associated with the evolution of pesticide resis-tance in arthropods.In this study,transcriptome sequencing was performed in 3 different resistance levels(low,L;medium,M;and high,H)of cyflumetofen-resistant strain(YN-CyR).A total of 1685 genes,including 97 detoxification enzyme genes,were upregulated in all 3 stages,of which 192 genes,including 11 detoxification enzyme genes,showed a continuous increase in expression level with resistance development(L to H).RNA in-terference experiments showed that overexpression of 7 genes(CYP392A1,TcGSTd05,CCE06,CYP389A1,TcGSTz01,CCE59,and CYP389C2)is involved in the development of cyflumetofen resistance in Tetranychus cinnabarinus.The recombinant CYP392A1 can effectively metabolize cyflumetofen,while CCE06 can bind and sequester cyflumetofen in vitro.We compared 2 methods for rapid screening of resistance molecular markers,in-cluding short-term induction and 1-time high-dose selection.Two detoxification enzyme genes were upregulated in the field susceptible strain(YN-S)by induction with 20%lethal concentration(LC2o)of cyflumetofen.However,16 detoxification enzyme genes were up-regulated by 1-time selection with LCso of cyflumetofen.Interestingly,the 16 genes were overexpressed in all 3 resistance stages.These results indicated that 1685 genes that were upregulated at the L stage constituted the basis of cyflumetofen resistance,of which 192 genes in which upregulation continued to increase were the main driving force for the de-velopment of resistance.Moreover,the 1-time high-dose selection is an efficient way to rapidly obtain the resistance-related genes that can aid in the development of resistance markers and resistance management in mites.

关键词： change rules cyflumetofen detoxification enzyme genes resistance molec-ular markers Tetranychus cinnabarinus

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Transcriptome dysregulation in hyper-progressive disease samples with immune checkpoint blockade

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Chinese Medical Journal 2023年第24期136卷 3019-3021页

作者： Dan Xue Tengteng Zhu Hongguang lin peilin Guo Mengling Li Mei’e Yu Fan Yang Sheng Yang Xiangqi Chen Department of Respiratory Medicine Fujian Medical University Union HospitalFuzhouFujian 350000China Department of Geriatrics Fujian Medical University Union HospitalFuzhouFujian 350000China Hongqiao International Institute of Medicine Shanghai Tongren Hospital/Clinical Research InstituteShanghai Jiao Tong University School of MedicineShanghai 200025China Department of Oncology Fujian Medical University Union HospitalFuzhouFujian 350000China Fujian Key Laboratory of Translational Research in Cancer and Neurodegenerative Diseases FuzhouFujian 350122China

To the Editor:Tumor immunotherapy has made rapid progress in recent years.However,immune checkpoint blockade(ICB)therapy may cause clinical symptoms of hyper-progressive disease(HPD),especially for patients with alterations or amplifications in driver genes,such as mouse double minute 2(MDM2),epidermal growth factor receptor(EGFR),and fibroblast growth factor 4(FGF4).Recently,Kamada et al[1]investigated the molecular mechanism of HPD to explore whether immune checkpoint inhibition caused HPD in patients in clinical trials.Ki67+effector regulatory T cells(eTregs)in HPD patients were found to be increased after PD-1 monoclonal antibody treatment.Other studies have also found that immune cells in the tumor microenvironment of patients with HPD show obvious changes before and after treatment.[2]Tumor microenvironment changes in HPD imply the potential presence of unknown gene interactions that promote rapid growth of tumor cells after cancer immunotherapy.

关键词： alterations clinical patients

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纳米材料在骨髓炎治疗中的应用研究进展

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中国修复重建外科杂志 2021年第5期35卷 648-655页

作者：王珮琳林浩东上海市第一人民医院骨科上海200080

目的对纳米材料在骨髓炎治疗中的相关应用研究进展进行综述,为骨髓炎的研究及临床治疗提供新思路。方法查阅近年国内外应用纳米材料治疗骨髓炎的相关文献,并进行分析和总结。结果目前,手术治疗和抗生素应用是骨髓炎的主要治疗方案,但存... 详细信息

目的对纳米材料在骨髓炎治疗中的相关应用研究进展进行综述,为骨髓炎的研究及临床治疗提供新思路。方法查阅近年国内外应用纳米材料治疗骨髓炎的相关文献,并进行分析和总结。结果目前,手术治疗和抗生素应用是骨髓炎的主要治疗方案,但存在抗生素耐药、骨缺损残留、局部药物有效浓度较低等诸多缺陷。应用纳米材料则能针对性地弥补上述缺陷。近年来,在骨髓炎治疗中纳米材料主要通过填充骨缺损、建立局部载药缓释系统以及自身抗菌等特性发挥作用。结论充分研究纳米材料相关特性,并选择有益材料制作载药系统或替代药物,为骨髓炎的治疗提供了全新思路及重要研究方向。

关键词：骨髓炎纳米材料骨缺损局部载药缓释系统成骨分化破骨分化抗菌

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Digital Preservation:A New Approach to Protecting Historical and Cultural Towns and Villages

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Contemporary Social Sciences 2018年第3期3卷 86-96页

作者： Liu peilin Deng Yunyuan lin Min(译) Zhang Congrong(译) School of Cities and Tourism Hengyang Normal University 不详

Digital technology has penetrated all aspects of modern life and is pushing society forward by profoundly changing the way people live and think.It can not only eternalize cultural heritage,but also allow relevant knowledge to be spread more quickly and effectively.China is home to a diverse array of historical and cultural towns and villages,which are an important cultural heritage.Digital preservation means leveraging technologies such as mapping,remote sensing,computers,3 D imaging,virtual reality,plus physical and chemical techniques to collect data to monitor,record,restore,and rebuild with the goal of preserving both their physical shapes and cultural legacies.

关键词： digitization historical and cultural towns and villages 3D technology virtual reality digital legacy

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Online dialogue of friendship associations of four countries with ancient civilization a success

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Voice of Friendship 2022年第3期 22-26页

作者： Wang peilin 不详

On June 24 the Online Dialogue of Friendship Associations of Four Countries with Ancient Civilizations was held successfully.It was initiated and hosted by the Chinese People’s Association for Friendship with Foreign Countries,with participation by China,India,Egypt and Greece.lin Songtian,president of the CPAFFC;George Floras,managing partner of the Belt and Road Associates in Greece.

关键词： Online initiated partner

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金刚线多线切割机在天然石材荒料板材加工领域的应用及产业化可行性分析

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机械管理开发 2021年第12期36卷 303-304,307页

作者：林培林厦门品河机械制造有限公司福建厦门361000

阐述了天然石材在传统设备加工过程中所碰到的问题和痛点,并就金刚线多线切割机在天然石材荒料板材加工领域的应用及产业化可行性作了分析。探讨金刚线在产业化过程中碰到的困难及解决方案,针对石材加工过程中的特点并且结合金刚线的特... 详细信息

阐述了天然石材在传统设备加工过程中所碰到的问题和痛点,并就金刚线多线切割机在天然石材荒料板材加工领域的应用及产业化可行性作了分析。探讨金刚线在产业化过程中碰到的困难及解决方案,针对石材加工过程中的特点并且结合金刚线的特殊性,提出了金刚线切割石材设备的研究方案。

关键词：金刚线多线切割机石材超薄加工锯缝

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Expression of a New hemA Gene from Agrobacterium radiobacter in Escherichia coli for 5-Aminolevulinate Production

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Chinese Journal of Chemical Engineering 2005年第4期13卷 522-528页

作者： LIU Xiaoxia（刘晓侠） lin Jianping（林建平） QIN Gang（秦钢） CEN peilin（岑沛霖） Institute of Bioengineering College of Materials Science and Chemical Engineering Zhejiang University Hangzhou 310027 China

A new hemA gene encoding 5-aminolevulinate (ALA) synthase was cloned from Agrobacterium ra- diobacter zju-0121. The ALA synthase catalyzes the pyridoxal phosphate-dependent condensation of succinyl coen- zyme A (succinyl-CoA) and glycine to produce ALA. Four plasmids carrying the A, radiobacter hemA gene were transformed into different E. coli strains. The effects of both genetic and physiological factors on the expression of ALA synthase and ALA production were studied. The results indicated that the final intracellular activity of ALA synthase and the production of ALA in different expression systems varied largely. Among them, the recombinant E. coli BL21 (DE3) harboring the expression plasmid pET28-A. R-hemA was the most suitable one. The effects of isopropyl-β-D-thiogalactopyranoside (IPTG) addition time, IPTG concentration, culture temperature and the initial concentration of precursors and glucose on the ALA production were also evaluated. The expressed ALA synthase accounted for about 23.7% of the intracellular soluble protein. The highest specific activity of ALA syn- thase was 13.8nmol·min-1·mg-1 of intracellular soluble protein. In the batch culture of the recombinant E. coli, the extracellular ALA concentration reached 0.9 g·L-1.

关键词： hemA基因 5-氨基乙醛丙醇大肠杆菌基因表达

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Coronary heart disease： incidence, risk factors and interventions in Jiaozhou of Shandong province

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Chinese Medical Journal 2014年第12期127卷 2275-2278页

作者： Yu Hua Li Dan Chu Xianming An Yi Song Tongxun Feng Huixin Lin Peilin Wang Tao Jiang Shaoyan Guo linlin Xu Fengqiang Liu Zhengke Yang Bin Department of Cardiology Affiliated Cardiovascular Hospital of Qingdao UniversityQingdaoShandong 266071China Department of Cardiology Affiliated Hospital of Qingdao UniversityQingdaoShandong 266042China Department of Cardiology Jiaozhou Peoples HospitalQingdaoShandong 266300China Department of Cardiology Jiaozhou Puji Central Health InstituteQingdaoShandong 266326China

Background Coronary heart disease (CHD) is the most common type of heart disease and cause of heart attacks.This study investigated the epidemiological characteristics of CHD and its risk factors in Jiaozhou,Shandong province,to ultimately find a way of reducing the prevalence of cardiovascular disease,and to provide a theoretical basis for establishing a cardiovascular disease management path under the regional medical collaborative mechanism.Methods A questionnaire survey was performed including 1 952 people aged 35 years or older who were questioned by means of stratified,cluster,proportional sampling to investigate the prevalence of CHD and its risk factors.The data were inputted into SPSS11.0 statistical software for processing and analysis.We advised the local medical institutions to establish health files for the residents with CHD and risk factors.They were followed up regularly.Their risk factors and life-style were monitored,and advice was given as to proper medications.Green channels were established,and the patients were transmitted in a timely manner to superior hospitals for better treatment if the necessary treatments were not available in the local hospitals.The control of risk factors was observed after the follow-up for half a year.Results In Jiaozhou,the rates of coronary artery disease,hypertension,diabetes,hyperlipidemia and overweight were 8.15%,28.54%,11.43%,35.46%,and 18.70% respectively.The rates of hypertension,diabetes,hyperlipidemia and overweight were higher than the data published in "The report of Chinese cardiovascular disease 2012"; which are 24%,9.7%,18.6%,and 9.7%,respectively.The control of risk factors improved significantly after the guidance of the residents lifestyle and medication for six months.Conclusions The high prevalence of coronary artery disease in Jiaozhou is closely related to age,gender,diet structure,family history of cardiovascular disease,hypertension,diabetes,hyperlipidemia,overweight,and unhealthy lifestyle.Under the regional medical coordination mechanism,

关键词：冠状动脉疾病危险因素心脏疾病发病率山东省干预措施胶州市心血管疾病

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近六十年来明清时期孔府土地问题研究述评

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中国史研究动态 2019年第5期 44-51页

作者：吴佩林林家伟曲阜师范大学历史文化学院

孔氏,被尊称为“天下第一家”,其地位世袭罔替,不随王朝更迭&现保留有大量的原始档案,涵盖内容宏富,蕴含价值巨大,是我们了解古代社会特别是明清时期的第一手珍贵文献。孔府由于世受皇恩,特别在明清时期,拥有大量田产,相关文献也有记载... 详细信息

孔氏,被尊称为“天下第一家”,其地位世袭罔替,不随王朝更迭&现保留有大量的原始档案,涵盖内容宏富,蕴含价值巨大,是我们了解古代社会特别是明清时期的第一手珍贵文献。孔府由于世受皇恩,特别在明清时期,拥有大量田产,相关文献也有记载。20世纪60年代以来,学界利用这些文献取得了一系列成果,推进了孔府土地制度及相关问题的研究。本文拟对这些成果作一述评,以期对此问题有一个更加清晰明了的认识。

关键词：明清时期土地问题孔府述评相关文献原始档案古代社会 60年代

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应急救援队伍体系的法治化完善路径

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中国应急救援 2023年第3期 4-8,15页

作者：林鸿潮杨佩琳中国政法大学应急管理法律与政策研究基地中国政法大学法学院

我国目前已经形成了由国家、地方、基层、社会四大类应急救援队伍组成的应急救援力量体系,各类应急救援队伍的建设基本上实现了有法可依。但是,仍有多支应急救援队伍及其人员的地位、职责、身份尚不明确,对应急救援队伍及其人员的保障... 详细信息

我国目前已经形成了由国家、地方、基层、社会四大类应急救援队伍组成的应急救援力量体系,各类应急救援队伍的建设基本上实现了有法可依。但是,仍有多支应急救援队伍及其人员的地位、职责、身份尚不明确,对应急救援队伍及其人员的保障激励措施远不完善,各类队伍参与应急救援的协同机制也不够顺畅。应当在法治化轨道上推进应急救援队伍建设,加快出台《应急救援队伍管理法》,重点明确各类应急救援队伍的角色定位,完善应急救援队伍和人员的保障激励措施,健全应急救援的协同机制。

关键词：应急救援队伍应急救援能力法治化

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