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Chinese Medical Journal 2009年第4期122卷 460-465页

作者： lIU Hong YE lin WANG Qing-wei YAN Qiu-xia YU Jin-ming Cancer Research -Center Qilu Hospital Shandong UniversityJinan Shandong 250012 China Department of Health Care Shandong Provincial Hospital JinanShandong 250012 China Department of Radiation Oncology Shandong Cancer HospitalJinan Shandong 250117 China

Back.round Hepatitis B virus （HBV） x protein （HBx） in HepG2 cells causes a moderate decrease in proteolysis activity of the proteasome. A highly conserved k.nitz-type serine protease inhibitor domain within 154 amino acid residues of HBx has been identified. In this study, a peptide chain derived from the k.nitz domain （Pk.） was used to study its effect on the cell cycle and apoptosis of HepG2 cells, and investigated the function of Pk. on the activities of proteasomes and AAA-ATPase p97, which involves in the ubiquitin-proteasome protein degradation pathway. Methods The Pk. peptide （Phe-Val.l.u-Gly-Gly-Cys-Arg-His-lys） was chemically synthesized. MTT assays were used to determine the effects of Pk. on HepG2 cell growth. Mouse anti-p97 antibody was developed for Western blotting to detect the expression of p97. ATPase activity of proteasomes was measured using a colorimetric assay. Peptidase activities of proteasomes were analyzed with various peptidase-specific fluorogenic peptide substrates. Flow cytometry was used to determinate cell cycle phase and apoptosis. Results Viability of HepG2 cells decreased in a Pk.-dose-dependent manner. Cells exhibited significant cytotoxicity in the presence of 15 mmol.l.of Pk.. Western blotting analysis showed that expression of p97 was suppressed in HepG2 cells treated with Pk. compared to untreated cells. The ATPase activity of proteasomes from immunoprecipitates of HepG2 cells pretreated with Pk. was apparently decreased. Chymotryptic activity of proteasomes in HepG2 cells was significantly inhibited by 10mmol.l.Pk.; tryptic activity and peptidyl.l.tamyl peptide hydroiase activity of proteasomes were less inhibited by Pk. than chymotryptic activity. The cell cycle phase of HepG2 cells treated with Pk. for 36 hours was block.d largely at the G0-G1 phase, while untreated control cells were mainly in S phase. Pk. also significantly induced apoptosis. Conclusions The peptide derived from k.nitz domain of HBx protein induces H

关键词： peptides ubiquitin proteasome p97 valosin-containing protein carcinoma hepatocell.l.r apoptosis

Effects of glutamate and nimodipine on survival rate of embryonic rat neuronal stem cells cultured in vitro

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Neural Regeneration Research 2008年第12期3卷 1286-1289页

作者： Xiaohong lu Hailong Fu Qiang Sun li Cui Dihui Ma Weihong lin Department of Neurology First Hospital Jinlin University Changchun 1300021 Jilin Province China

BACk.ROUND： At least three types of calcium ion channel （T, N, and l） have been recognized in nerve ceils, but only the l type of channel is sensitive to drugs. Theoretically, nimodipine can lead to l-type channel inactivation and prevent calcium ion inflow, thereby exhibiting protective effects on nerve cells. OBJECTIVE： To observe the protective effects of nimodipine on glutamate-induced injury to embryonic rat neural stem cells, and to mak. a comparison with Mk.801, a nonselective glutamate receptor antagonist. DESIGN, TIME AND SETTING： The present in vitro experiment pertaining to neural stem cells was performed at the Department of Neurology, First Hospital, Jilin University between January 2005 and December 2006. MATERIAlS： Glutamate was sourced from the Shanghai Biological Research Institute of the Chinese Academy of Sciences. Nimodipine was provided by Bayer Company, Germany. Brain tissue was tak.n from Wistar rats on day 15 of gestation for isolation and culture of neural stem cells. METHODS： Passage 2 neural stem cell spheres were tak.n for preparation of single cell suspension. The prepared single cell suspension was divided into 4 groups：（1） Normal control, normally cultured. （2） Glutamate, cultured with 50, 100, 200, 500, and 1 000 μmol.l.glutamate. （3） Nimodipine, received a 30- minute nimodipine [1×（10^-8-10^-2） g/l] culture followed by a glutamate （200 μmol.l. treatment step. （4） Mk.801, given as 30- minute Mk.801 （100 μmol.l. culture, followed by a glutamate （200 μmol.l. treatment step. MAIN OUTCOME MEASURES： Determination of glutamate-induced cell death by methyl thiazol.l.tetrazolium （MTT） assay; calculation of neural stem cell survival rate following addition of nimodipine. RESUlTS： The survival rate of neural stem cells was approximately 25.26% following 24 hour 50 μmol.l.glutamate culture and gradually decreased as the glutamate dose increased （P 〈 0.05 0.01）. Only 9.27% of neural stem cells survived when the glutamate dose was 1000 μmol.l. Th

关键词： neural stem cells culture glutamate injury nimodipine protective effects

Mn、S共掺杂g-C_(3)N_(4)活化过硫酸盐降解四环素的影响因素探究

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广州化工 2024年第6期52卷 120-123,138页

作者：黄继明刘润清皮军龙泽荣何炳林李方庆李莲王清宇铜仁学院材料与化学工程学院贵州铜仁554300

四环素因其应用广泛、难以自然降解性以及潜在的生态风险,成为水环境污染物中的重点关注对象。开发低成本、高效的光催化剂活化过硫酸氢钾(PMS)降解有机污染物被认为是治理环境污染的重要途径。本研究采用简单的煅烧法制备了Mn、S共掺杂... 详细信息

四环素因其应用广泛、难以自然降解性以及潜在的生态风险,成为水环境污染物中的重点关注对象。开发低成本、高效的光催化剂活化过硫酸氢钾(PMS)降解有机污染物被认为是治理环境污染的重要途径。本研究采用简单的煅烧法制备了Mn、S共掺杂g-C_(3)N_(4)催化剂,用于协同光活化PMS降解四环素。采用X射线衍射仪(XRD)对其进行表征,设计单因素试验探究Mn、S共掺杂g-C_(3)N_(4)降解四环素效果和最佳条件。单因素试验结果表明,最佳Mn、S共掺杂g-C_(3)N_(4)投加量为0.2 g/l,过硫酸氢钾浓度为5 mmol.l.四环素溶液初始pH为3,初始浓度为10 mg/l(100 ml)时去除效果最好,四环素去除率为90.4%。这项研究为使用g-C_(3)N_(4)基光催化剂降解抗生素提供了一种新的策略。

关键词： Mn、S掺杂石墨相氮化碳过硫酸盐四环素光催化降解

Tubulin detyrosination promotes human trophoblast syncytium formation

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Journal of Molecular Cell Biology 2019年第11期11卷 967-978页

作者： Rui Wang Ruoxuan Yu Cheng Zhu Hai-Yan lin Xiaoyin lu Hongmei Wang State k.y laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Department of Experimental Radiation Oncology the University of Texas MD Anderson Cancer CenterHoustonTXUSA University of Chinese Academy of Sciences BeijingChina

Human trophoblast syncytialization is one of the most important yet least understood events during placental *** this study,we found that detyrosinated a-tubulin(detyr-a-tub),which is negatively regulated by tubulin tyrosine Ugase(TTl),was elevated during human placental cytotrophoblast ***,relatively high expression of TTl protein was observed in first-trimester human placental cytotrophoblast cells,but fusing trophoblast cells exhibited much lower levels of T T l Notably,fusion of preeclamptic cytotrophoblast cells was compromised but could be partially rescued by k.ock.own of TTl ***,chronic downregulation of TTl in trophoblast cells resulted in significantly elevated expression of *** of detyr-a-tub thus contributed to the cell surface localization of the fusogenic protein Syncytin-2 and the gap junction protein Connexin 43(Cx43),which in turn promoted successful fusion between trophoblast *** together,the results suggest that tubulin detyrosination plays an essential role in human trophoblast fusogenic protein aggregation and *** tubulin detyrosination leads to defects in syncytialization and potentially to the onset of preeclampsia.

关键词： TTl detyrosination syncytialization preeclampsia placenta syncytin

Evaluation of The Interaction between Netropsin and Double Stranded DNA by Capillary Zone Electrophoresis

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Chinese Chemical.l.tters 2003年第3期14卷 304-307页

作者： Xin Ya HE Da Zhi lI Ai Ye lIANG Bing Cheng lin Dalian Institute of Chemical Physics Chinese Academy of SciencesDalian 116023

Capillary zone electrophoresis (CZE) was applied to study the interaction between netropsin and a 14mer double stranded DNA (dsDNA). The binding constant of this interaction calculated from Scatchard plot was (1.070.10)×105 (mol.l.-1. The binding stoichiometry was 1:1. The use of polyacrylamide coated capillary showed better effect in the analysis of DNA than noncoated capillary.

关键词： Interaction netropsin DNA capillary zone electrophoresis.

曹雪涛院士科研团队论文荣膺中国百篇最具影响国际学术论文--长链非编码RNA1nc－DC通过STAT3调控人DC的分化和功能

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中国肿瘤生物治疗杂志 2016年第1期23卷 I0001-I0002页

作者： wang p xue y han y lin l wu c xu s jiang z xu j liu q cao x

树突状细胞（DC）是免疫系统中重要的抗原提呈细胞，其成熟和功能状态决定着免疫应答的走向和强度。哺乳动物基因组能够转录产生大量的长链非编码RNA（long noncoding RNA，IncRNA），其在免疫系统中的功能还知之甚少。曹雪涛院十科研... 详细信息

树突状细胞（DC）是免疫系统中重要的抗原提呈细胞，其成熟和功能状态决定着免疫应答的走向和强度。哺乳动物基因组能够转录产生大量的长链非编码RNA（long noncoding RNA，IncRNA），其在免疫系统中的功能还知之甚少。曹雪涛院十科研团队王品副教授课题组采用了转录组芯片二代测序高通量筛选方法、RNA-pulldown、ChIP-seq等技术展开了对IncRNA在DC分化和功能中作用的研究，发现并鉴定了特异性高表达于DC的1ncRNA，命名为1nc-DC，其可通过抑制STAT3去磷酸化，从而促进DC分化和功能。该研究成果发表于Science杂志[2014，344（6181）：310-313]，并于2015年12月被评为“中国百篇最具影响国际学术论文”。

关键词： STAT3 学术论文科研团队曹雪涛 DC 分化编码长链

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煤矿新工人早期肺通气功能的变化

中华劳动卫生职业病杂志 2005年第2期23卷 105-108页

作者：彭开良 Mei-lin Wang 杜庆国李亚东 Michael D. Attfield 韩桂海 Edward l. Petsonk.李绍奎吴植恩 430030 武汉华中科技大学同济医学院劳动卫生与环境卫生学系美国职业安全卫生研究所(NIOSH) Division of Respiratory Disease StudiesMorgantownWVUSA 江苏省徐州矿务集团职业病防治院

目的　研究煤矿粉尘对新工人肺通气功能的早期影响。方法　选择徐州矿务集团新招收的男性工人2 87人为矿工组,选择该集团技工学校在校男生132人为对照组。调查内容包括个人基本资料、家族疾病史、职业史、吸烟史、作业场所粉尘浓度、肺... 详细信息

目的　研究煤矿粉尘对新工人肺通气功能的早期影响。方法　选择徐州矿务集团新招收的男性工人2 87人为矿工组,选择该集团技工学校在校男生132人为对照组。调查内容包括个人基本资料、家族疾病史、职业史、吸烟史、作业场所粉尘浓度、肺通气功能随访测定。前瞻性队列调查为期3年,每半个月测定作业场所的总粉尘和呼吸性粉尘浓度;定期测定两组人群的用力肺活量(FVC)和第一秒用力呼气量(FEV1)。结果　矿工作业场所的总粉尘平均浓度为2 3.8mg/m3 ,呼吸性粉尘平均浓度为8.9mg/m3 ,均超过国家卫生标准。接尘第1年,矿工组FVC(5 .19l)高于对照组(4.92l) ,差异有统计学意义(P 0 .0 5 )。接尘前矿工组FEV1[(4.4 8±0 .4 9)l]高于对照组[(4.2 8±0 .4 8)l];接尘1年时FEV1下降至4 .2 5l ;第2年和第3年FEV1(4.34l)低于对照组(4.5 6l) ,差异有统计学意义(P l)高于不吸烟者(83、91ml)。FVC、FEV1与年龄、身高和体重存在一定相关。结论　粉尘对煤矿新工人的早期肺通气功能有明显影响,FEV1下降比FVC更为明显;吸烟可加重粉尘对肺通气功能的损害。

关键词：采煤粉尘肺通气队列研究

Construction of BITC loaded fish sk.n gelatin-luteolin compound emulsion delivery system and its digestion and absorption characteristics

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Construction of BITC loaded fish skin gelatin-luteolin compo...

“猛香系”杜长大三元猪相关屠宰性能、肉质测定的分析

中国营养学会第十五届全国营养科学大会

作者： Zhiyu liu Wenwen liu Songyi lin Yue Tang School of Food Science and Technology Dalian Polytechnic University

The purpose of this study was to construct an emulsion system that can be used to improve the stability of nutrients in the gastrointestinal tract. Fish sk.n gelatin(FSG) and luteolin(lUT) were used as the composite emulsifier and benzyl isothiocyanate(BITC) as a model of nutrient delivery to construct a stable emulsion. The storage stability, antioxidant stability, protective effect of BITC, digestion and absorption characteristics of the emulsions were investigated. Methods Firstly, based on the results of the previous experiments and the recommended daily intak. of lUT, the BITC emulsion delivery system was constructed with 0.06% lUT and 1% FSG as the composite carrier. The changes of particle size, zeta potential, and BITC retention during storage at 4 °C for 0, 1, 4, 7, and 14 days were investigated by laser particle size measurement and high-performance liquid chromatography(HPlC). The DPPH and ABTS scavenging rates were used as indicators to analyse the antioxidant capacity of emulsion system given by lUT during storage. Secondly, the microstructural changes of the emulsions at each digestion stage were observed by simulating gastrointestinal(GIT) model in vitro combined with laser confocal microscopy(ClSM) and cold field scanning electron microscopy(cryoSEM). Furthermore, the lipid digestion, the retention rate and bioavailability of BITC in the emulsions after simulated GIT digestion in vitro were determined by alk.line titration and high-performance liquid chromatography(HPlC).Finally, the retention rate at each digestive stage and absorption rate in each intestinal segment of BITC in the emulsions were examined by ex-vivo everted gut sac of rat intestine study to determine the intestinal absorption characteristics of BITC in the FSG-lUT emulsion delivery system. Results The results showed that, after 14 days of storage at 4 °C, the particle size of FSG-lUT emulsion was significantly lower than that of control(FSG emulsion), the zeta potential of the FSG-l

关键词： Fish sk.n gelatin luteolin Benzyl isothiocyanate Bioavailability

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养猪 2024年第3期 15-18页

作者：李敏张云鹏潘丽赵元张琪郝文杰李卓珊哈斯朝鲁董雅宏张树敏吉林农业大学动物科学技术学院吉林长春130118 吉林省农业科学院吉林长春130119 内蒙古赤峰家育种业科技有限公司内蒙古赤峰024005

为充分研究家育“猛香系”杜×长×大三元猪的屠宰性能及肉品质,对其进行屠宰实验及品质检验。选择30头同一饲养环境、管理条件的体重约为115 k.三元猪进行屠宰测定,分析其屠宰性能、胴体性状以及肉品品质。结果表明:胴体指标分析得出,... 详细信息

为充分研究家育“猛香系”杜×长×大三元猪的屠宰性能及肉品质,对其进行屠宰实验及品质检验。选择30头同一饲养环境、管理条件的体重约为115 k.三元猪进行屠宰测定,分析其屠宰性能、胴体性状以及肉品品质。结果表明:胴体指标分析得出,胴体均重为85.25 k.,屠宰率为70.25%,胴体长为97.45 cm,背膘厚为23.59 mm,皮厚为2.66 mm,眼肌面积为49.31 cm^(2),腿臀比例为33.19%;肉质指标分析得出,肌肉色值l_(24)42.93,pH_(1)值6.37,pH_(24)值为5.76,48 h滴水损失2.53%,系水力92.58%。实验为进一步提高和改善三元猪的胴体品质和优质选育育种方案提供参考的数据支撑。

关键词： “猛香系”猪杜长大三元猪肉质测定屠宰性能品质检验