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Early methylprednisolone impact treatment for sensory and motor function recovery in patients with acute spinal cord injury A self-control study

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Neural Regeneration Research 2008年第5期3卷 577-580页

作者： Chao Zhuang liming Wang Yan xu Department of Orthopaedics Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing 210006 Jiangsu Province China

BACKGROUND： For the treatment of spinal cord injury, any pathological changes of the injured tissue should be primarily corrected or reversed. Any remaining fibrous function and neurons with intact structure should be retained, and the toxic substances caused by ischemia-hypoxia following spinal cord injury, should be eliminated to create a favorable environment that would promote neural functional recovery. OBJECTIVE： This study was designed to investigate the effects of the impact of early methylprednisolone-treatment on the sensory and motor function recovery in patients with acute spinal cord injury. DESIGN： A self-control observation. SETTING： Department of Spine Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, China. PARTICIPANTS： Forty-three patients with acute spinal cord injury were admitted to the Department of Spine Surgery, First Affiliated Hospital of Nanjing Medical University, between October 2005 and September 2007. These patients were recruited for the present study. The patients comprised 33 males and 10 females, and all met with the inclusive criteria namely, the time between suffering from acute spinal cord injury and receiving treatment was less than or equal to eight hours. METHODS： According to the protocol determined by the State Second Conference of Acute Spinal Cord Injury of USA, all patients received the drop-wise administration of a 30-mg/kg dose of methylprednisolone （H200040339, 500 mg/bottle, Pharmacia N.V/S.A, Belgium） for 15 minutes within 8 hours post injury. After a 45-minute interval, methylprednisolone was administered at 5.4 mg/kg/h for 23 hours. MAIN OUTCOME MEASURES： Prior to and post treatment, acupuncture sense and light touch scoring were performed at 28 dermatomic area key points, including occipital tuberosity and supraclavicular fossa. At the same time, motor scoring of key muscles among 10 pairs of sarcomeres was also performed. RESULTS： All 43 patients participated in the fi

关键词： methylprednisolone acute spinal cord injury sensory and motor function

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Immune risk assessment of residual αGal in xenogeneic decellularized cornea using GTKO mice

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Regenerative Biomaterials 2020年第4期7卷 427-434页

作者： Liang Chen Lina Wei Anliang Shao liming xu Department of Medical Device National Institutes for Food and Drug ControlHuatuo Road 31Biomedical Production ZoneDaxing District102629 BeijingChina

The xenogeneic decellularized corneal matrix(DCM)was expected to be used in lamellar keratoplasty in clinic as the substitute of allogeneic *** decellularization treatment,the remaining risk of xenograft rejection needed to be *** galactose-α1,3-galactose,as the most abundant and closely rejection-related xenogeneic antigen,should be one of the important factors concerned in immunological *** this study,residual αGal in the DCM was first determined by an enzyme-linked immunosorbent assay method with qualified accuracy and *** the DCM was implanted subcutaneously into the α1,3-galactosyltransferase gene-knockout(GTKO)mice,accompanied by the implantation in the wild-type C57BL/6 mice as a *** total serum antibody levels,anti-Gal antibody levels,inflammatory cytokines and ratios of splenic lymphocyte subtypes were detected and the histopathological analysis of implants were performed to systematically evaluate the immune *** experimental result showed the fresh porcine corneal matrix samples had(9.90±1.54)×10^12 αGal epitope per mg while the content of residual aGal in the DCM was(7.90±2.00)×10^12 epitope per *** GTKO mice had similar potential of reaction to immune stimulation to that of wild-type C57BL/6 *** 4 weeks after implantation of DCM,in WT mice and GTKO mice there were both innate immunity response to the DCM characterized by macrophage *** the elevations of anti-Gal IgG level and the percentage of splenic natural killer cells were only detected in GTKO *** changes were thought to be pertinent to the residual αGal antigen,which could not be detected in WT *** further αGal antibody-mediated cellular immunity and significant changes of serum cytokine contents were found in GTKO mice,which perhaps suggested that the immune reactions to the DCM after 4 weeks of implantation were moderate and had minor effect on the survival of the corneal graft.

关键词： decellularized corneal matrix immune risk galactose-α1,3-galactose(aGal) GTKO mice

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Quantitatively relating magnetic resonance T_(1)and T_(2)to glycosaminoglycan and collagen concentrations mediated by penetrated contrast agents and biomacromolecule-bound water

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Regenerative Biomaterials 2023年第1期10卷 871-888页

作者： Jingming Gao Xian xu Xiaoye Yu Ye Fu Hongjie Zhang Siyi Gu Dinglingge Cao Quanyi Guo liming xu Jiandong Ding State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityShanghai 200438China Department of Radiology The SecondMedical Center&National Clinical Research Center for Geriatric DiseasesChinese PLA General HospitalBeijing 100853China Institute of Orthopedics The First Medical CenterChinese PLA General HospitalBeijing Key Lab of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma and War Injuries of PLABeijing 100853China Institute for Medical Device Control National Institutes for Food and Drug ControlBeijing 102629China

Magnetic resonance imaging(MRI)is a promising non-invasive method to assess cartilage regeneration based on the quantitative relationship between MRI features and concentrations of the major components in the extracellular matrix(ECM).To this end,in vitro experiments are performed to investigate the relationship and reveal the underlying mechanism.A series of collagen(COL)and glycosaminoglycan(GAG)solutions at different concentrations are prepared,and T_(1)and T_(2)relaxation times are measured with or without a contrast agent(Gd-DTPA2−)by *** transform infrared spectrometry is also used to measure the contents of biomacromolecule-bound water and other water,allowing theoretical derivation of the relationship between biomacromolecules and the resulting T_(2)*** has been revealed that the MRI signal in the biomacromolecule aqueous systems is mainly influenced by the protons in hydrogens of biomacromolecule-bound water,which we divide into inner-bound water and outer-bound *** have also found that COL results in higher sensitivity of bound water than GAG in T_(2)*** to the charge effect,GAG regulates the penetration of the contrast agent during dialysis and has a more significant effect on T_(1)values than *** that COL and GAG are the most abundant biomacromolecules in the cartilage,this study is particularly useful for the real-time MRI-guided assessment of cartilage regeneration.A clinical case is reported as an in vivo demonstration,which is consistent with our in vitro *** established quantitative relation plays a critical academic role in establishing an international standard ISO/TS24560-1:2022‘Clinical evaluation of regenerative knee articular cartilage using delayed gadolinium-enhanced MRI of cartilage(dGEMRIC)and T_(2)mapping’drafted by us and approved by International Standard Organization.

关键词： magnetic resonance imaging collagen glycosaminoglycan bound water cartilage

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The Design of Wireless Intelligent Differential Pressure Sensor Based on WICN

The Design of Wireless Intelligent Differential Pressure Sen...

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第25届中国控制与决策会议

作者： Haikuan WANG Yao LI Jingqi FU liming xu Weihua BAO School of Mechatronic Engineering and Automation Shanghai University Shanghai Automation Instrumentation Co. LTD.

ISBN: (纸本)9781467355339

In this paper, a wireless intelligent differential pressure sensor with high-precision and multi-function is designed which integrated intelligent sensing and Wireless Industrial Control Network （WICN） technology. MSP430 is used to calculate the linear correction and digital temperature compensation, and other intelligent functions in the sensor. To realize the network communication, A Dynamic Slot Assigned adaptive MAC protocol （DSA-MAC） is implemented by CC2530 SoC , which is integrated on the interface panel of the sensor. The data transmission test of the sensor nodes shows that this design has a higher data package transmission rate, and can fulfill the requirements of industry applications.

关键词： WICN Differential Pressure Sensor RBF Neural Network CC2530 DSA-MAC

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Dek35 Encodes a PPR Protein that Affects cis-Splicing of Mitochondrial nad4 Intron 1 and Seed Development in Maize

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Molecular Plant 2017年第3期10卷 427-441页

作者： Xinze Chen Fan Feng Weiwei Qi liming xu Dongsheng Yao Qun Wang Rentao Song Shanghai Key Laboratory of Bio-Energy Crops School of Life Sciences Shanghai University Shanghai 200444 China National Maize Improvement Center of China China Agricultural University Beijing 100193 China Coordinated Crop Biology Research Center (CBRC) Beijing 100193 China

In higher plants, the splicing of organelle-encoded mRNA involves a complex collaboration with nuclear- encoded proteins. Pentatricopeptide repeat （PPR） proteins have been implicated in these RNA-protein interactions. In this study, we performed the cloning and functional characterization of maize Defective kernel 35 （Dek35）. The dek35-ref mutant is a lethal-seed mutant with developmental deficiency. Dek35 was cloned through Mutator tag isolation and further confirmed by four additional independent mutant alleles. Dek35 encodes an P-type PPR protein that targets the mitochondria. The dek35 mutation causes significant reduction in the accumulation of DEK35 proteins and reduced splicing efficiency of mitochon- drial nad4 intron 1. Analysis of mitochondrial complex in dek35 immature seeds indicated severe deficiency in the complex I assembly and NADH dehydrogenase activity. Transcriptome analysis of dek35 endosperm revealed enhanced expression of genes involved in the alternative respiratory pathway and extensive differentially expressed genes related to mitochondrial function and activity. Collectively, these results indicate that Dek35 encodes an PPR protein that affects the cis-splicing of mitochondrial nad4 intron 1 and is required for mitochondrial function and seed development.

关键词： PPR splicing mitochondria NADH maize

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HQ-Tree： A Distributed Spatial Index Based on Hadoop

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China Communications 2014年第7期11卷 128-141页

作者： FENG Jun TANG Zhixian WEI Mian xu liming College of Computer and Information Hohai University Nanjing 210098 Jiangsu Province R R. China

In this paper,we propose a novel spatial data index based on Hadoop:*** HQ-Tree,we use PR QuadTrec to solve the problem of poor efficiency in parallel processing,which is caused by data insertion order and space *** the problem that HDFS cannot support random write,we propose an updating mechanism,called "Copy Write",to support the index ***,HQ-Tree employs a two-level index caching mechanism to reduce the cost of network transferring and I/O ***,we develop MapReduce-based algorithms,which are able to significantly enhance the efficiency of index creation and *** results demonstrate the effectiveness of our methods.

关键词： spatial index quadtree hadoop mapreduce

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Magnetic resonance imaging for non-invasive clinical evaluation of normal and regenerated cartilage

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Regenerative Biomaterials 2021年第5期8卷 50-62页

作者： Xian xu Jingming Gao Shuyun Liu Liang Chen Min Chen Xiaoye Yu Ning Ma Jun Zhang Xiaobin Chen Lisen Zhong Lin Yu liming xu Quanyi Guo Jiandong Ding Department of Radiology The Second Medical Center&National Clinical Research Center of Geriatric DiseasesChinese PLA General HospitalNo.28 Fuxing RoadHaidian DistrictBeijing 100853China State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular ScienceFudan UniversityNo.2005 Songhu RoadYangpu DistrictShanghai 200438China Institute of Orthopedics The First Medical CenterChinese PLA General HospitalBeijing Key Lab of Regenerative Medicine in OrthopedicsKey Laboratory of Musculoskeletal Trauma and War Injuries of PLANo.28 Fuxing RoadHaidian DistrictBeijing 100853China Institute for Medical Device Control National Institutes for Food and Drug ControlNo.31 Huatuo RoadDaxing DistrictBeijing 102629China

With the development of tissue engineering and regenerative medicine,it is much desired to establish bioimaging techniques to monitor the real-time regeneration efficacy in vivo in a non-invasive ***,we tried magnetic resonance imaging(MRI)to evaluate knee cartilage regeneration after implanting a biomaterial scaffold seeded with chondrocytes,namely,matrix-induced autologous chondrocyte implantation(MACI).After summary of the T2 mapping and the T1-related delayed gadolinium-enhanced MRI imaging of cartilage(dGEMRIC)in vitro and in vivo in the literature,these two MRI techniques were tried *** this study,18 patients were followed up for 1 *** was found that there was a significant difference between the regeneration site and the neighboring normal site(control),and the difference gradually diminished with regeneration time up to 1 year according to both the quantitative T1 and T2 MRI *** further established the correlation between the quantitative evaluation of MRI and the clinical Lysholm scores for the first ***,the MRI technique was confirmed to be a feasible semiquantitative yet non-invasive way to evaluate the in vivo regeneration of knee articular cartilage.

关键词： magnetic resonance imaging cartilage regeneration T2 mapping delayed gadolinium-enhanced MRI imaging tissue engineering

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Macroscopic on-site magnifier-based evaluation to estimate visible tissue core cut-off lengths using EUS-FNA with 22-gauge needles

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Chinese Medical Journal 2024年第4期137卷 493-495页

作者： Jialiang Huang Guilian Cheng Wei Wu liming xu Longjiang xu Duanmin Hu Department of Gastroenterology Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215004China Department of Pathology Second Affiliated Hospital of Soochow UniversitySuzhouJiangsu 215004China

To the Editor:Endoscopic ultrasound-guided fine needle aspiration(EUS-FNA)has replaced other high-risk invasive methods and has become the primary method of tissue collection around the gastrointestinal tract.^([1])To further improve the effectiveness and safety of EUS-FNA,pathologists may perform rapid on-site cytology evaluation(ROSE),but this process requires the presence of *** on-site evaluation(MOSE)has gained attention as it resolve the shortage of pathologist resources.^([2])Endoscopists can inspect the tissue core in real time and assess the adequacy of the punctured *** this study,we conducted a single-center and single-arm exploratory study to explore the value of MOSE in assessing specimen quality during magnifier-based EUS-FNA and to confirm the cut-off length of visible tissue core(VTC)for EUS-FNA to help endosonographers independently predict the adequacy of punctured *** VTC was defined as a white or incarnadine tissue core obtained from focal lesions with a relatively complete histological structure.

关键词： EUS needle visible

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Clinical and molecular significance of homologous recombination deficiency positive non-small cell lung cancer in Chinese population:An integrated genomic and transcriptional analysis

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Chinese Journal of Cancer Research 2024年第3期36卷 282-297页

作者： Yifei Wang Yidan Ma Lei He Jun Du Xiaoguang Li Peng Jiao Xiaonan Wu Xiaomao xu Wei Zhou Li Yang Jing Di Changbin Zhu liming xu Tianlin Sun Lin Li Dongge Liu Zheng Wang Department of Pathology Department of Minimally Invasive Tumor Therapies Center Department of Thoracic Surgery Department of Oncology Department of Respiratory and Critical Care Medicine Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Amoy Diagnostics Co. Ltd.Xiamen 361027China

Objective:The clinical significance of homologous recombination deficiency(HRD)in breast cancer,ovarian cancer,and prostate cancer has been established,but the value of HRD in non-small cell lung cancer(NSCLC)has not been fully *** study aimed to systematically analyze the HRD status of untreated NSCLC and its relationship with patient prognosis to further guide clinical ***:A total of 355 treatment-naïve NSCLC patients were retrospectively *** status was assessed using the AmoyDx Genomic Scar Score(GSS),with a score of≥50 considered ***,transcriptomic,tumor microenvironmental characteristics and prognosis between HRD-positive and HRDnegative patients were ***:Of the patients,25.1%(89/355)were *** to HRD-negative patients,HRDpositive patients had more somatic pathogenic homologous recombination repair(HRR)mutations,higher tumor mutation burden(TMB)(P<0.001),and fewer driver gene mutations(P<0.001).Furthermore,HRD-positive NSCLC had more amplifications in PI3K pathway and cell cycle genes,MET and MYC in epidermal growth factor receptor(EGFR)/anaplastic lymphoma kinase(ALK)mutant NSCLC,and more PIK3CA and AURKA in EGFR/ALK wild-type ***-positive NSCLC displayed higher tumor proliferation and immunosuppression ***-negative NSCLC showed activated signatures of major histocompatibility complex(MHC)-II,interferon(IFN)-γand effector memory CD8+T ***-positive patients had a worse prognosis and shorter progressionfree survival(PFS)to targeted therapy(first-and third-generation EGFR-TKIs)(P=0.042).Additionally,HRDpositive,EGFR/ALK wild-type patients showed a numerically lower response to platinum-free immunotherapy ***:Unique genomic and transcriptional characteristics were found in HRD-positive *** prognosis and poor response to EGFR-TKIs and immunotherapy were observed in HRD-positive *** study highlights potential actionable alterations in HRD

关键词： Non-small cell lung cancer homologous recombination deficiency genetic alterations transcriptional analysis tumor microenvironment prognosis

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基于同态滤波和K均值聚类算法的杨梅图像分割

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农业工程学报 2015年第14期31卷 202-208页

作者：徐黎明吕继东常州大学信息科学与工程学院常州213164 江苏城乡建设职业学院设备工程系常州213147

针对自然环境下光照不均杨梅果实分割效果不理想问题展开研究。应用同态滤波算法对HSV(色调hue,饱和度saturation,亮度value)颜色空间下杨梅图像V分量进行亮度增强,以补偿光线。而后针对彩色杨梅图像的颜色特征,结合Lab(L(亮度Lightness... 详细信息

针对自然环境下光照不均杨梅果实分割效果不理想问题展开研究。应用同态滤波算法对HSV(色调hue,饱和度saturation,亮度value)颜色空间下杨梅图像V分量进行亮度增强,以补偿光线。而后针对彩色杨梅图像的颜色特征,结合Lab(L(亮度Lightness),a(色度chromaticity,+a表示红色,-a表示绿色),b(色度chromaticity,+b表示黄色,-b表示蓝色)颜色空间a、和b分量的特点,应用K均值聚类算法在Lab颜色空间中对彩色杨梅图像进行分割。为了验证该算法的有效性,在100余幅图像中选用15幅因光照不均和生长状态不同而存在不同程度阴影影响的杨梅图像,进行了3组比较试验,先采用K均值聚类算法对光线补偿去除阴影前后的杨梅图像分割结果进行比较;接着,采用基于色差2*R-G-B自适应灰度阈值分割算法和K均值聚类算法2种不同分割算法对去除阴影后的杨梅图像分割结果进行比较;最后,与基于灰度变换法、直方图均衡化方法的图像增强法去除阴影的效果进行对比。试验结果表明,该文算法的分割误差、假阳性率、假阴性率平均值分别为3.78%,0.69%和6.8%,分别比光线补偿前降低了21.01,12.79和21.14个百分点;与基于色差(2*R-G-B)自适应灰度阈值分割算法相比,分割误差、假阳性率、假阴性率这3个指标的性能平均提高了12.93,1.45和7.11个百分点;与基于灰度变换法图像增强法比较表明,分割误差、假阳性率、假阴性率平均值分别降低了32.94,6.85和29.65个百分点,与直方图均衡化图像增强法相比,这3个值分别降低了24.92,6.12和33.06个百分点。通过试验结果图的主观判断和评价指标的定量分析,验证了该算法能有效地分割出杨梅目标,保证了杨梅目标在颜色、纹理和形状方面的完整度,研究结果为研究采摘机器人进行杨梅等果实的分割和识别提供参考。

关键词：图像分割算法水果同态滤波 K均值聚类杨梅

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