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Spatial heterogeneity of cyanobacterial communities and genetic variation of Microcystis populations within large,shallow eutrophic lakes(Lake Taihu and Lake Chaohu,China)

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Journal of Environmental Sciences 2012年第10期24卷 1832-1842页

作者： Yuanfeng Cai Fanxiang Kong limei shi Yang Yu State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China Graduate University of Chinese Academy of Sciences Beijing 100049China

Cyanobacteria, specifically Microcystis, usually form massive blooms in eutrophic freshwater lakes. Cyanobacterial samples were collected from eight sites of both Lake Taihu and Lake Chaohu in late summer to determine the diversity and distribution pattern of cyanobacteria and Microcystis in large, shallow, entropic lakes with significant spatial heterogeneity and long-term Microcystis bloom. Molecular methods based on denaturing gradient gel electrophoresis and clone library analysis were used. A similar heterogeneous distribution pattern of cyanobacteria in both lakes was observed. Most parts of these two lakes with high trophic level were dominated by Microcystis. However, in the regions with low trophic levels as well as low concentrations of chlorophyll a, Synechococcus occupied a considerable percentage. Different morphospecies and genotypes dominated the bloom-forming Microcystis populations in these two lakes. Microcystis viridis and Microcystis novacekii were dominant in Lake Chaohu, whereas Microcystis flos-aquae was dominant in Lake Taihu. Only 2 of thel3 Microcystis operational taxonomic units were shared between these two lakes. Analysis of molecular variance based on 16S to 23S internal transcribed spacer sequences indicated the significant genetic differentiation of Microcystis between these two lakes （Fst = 0.19, p 〈 0.001）. However, only 19.46% of the genetic variability was explained by the population variation between lakes, whereas most （80.54%） of the genetic variability occurred within the lakes. Phylogenetic analysis revealed no phylogeographic structure of Microcystis population in these two lakes, as illustrated by their cosmopolitan nature. Our results revealed that spatial heterogeneity within lakes has more impact on the cyanobacterial diversity than geographical isolation in a local scale.

关键词： cyanobacteda Microcystis Synechococcus bloom diversity

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Discovery of Novel Tricyclic 5H-Pyridazino[4,5-b]indoles as Potent Antitumor Agents： Design, Synthesis and Biological Evaluation

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Chemical Research in Chinese Universities 2015年第3期31卷 372-380页

作者： ZHAI Xin WANG limei shi Jiyue GONG Ping Key Laboratory of Structure-based Drug Design and Discovery Ministry of EducationShenyang Pharmaceutical University Shenyang 110016 P.R.China

A novel series of 5H-pyridazino[4,5-b]indoles（L-01-L-32） was synthesized and characterized by means of 1H NMR, MS and elemental analysis. The cytotoxicity of the target compounds against Bel-7402 and HT-1080 cell lines were evaluated by 3-（4,5-dimethylthiazol-2-y1）-2,5-diphenyltetrazolium bromide（MTT） assay. Most of them exhibited moderate to excellent cytotoxicity, and six compounds（L-04, L-06, L-18, L-20, L-21 and L-23） possessed dramatically increased cytotoxicity superior to Gefitinib. Of these initial hits, compound L-21 displayed remarkable cytotoxicity against the tested cell lines with half maximal inhibitory concentration（IC50） values of 4.6 and 2.1 μmol/L, respectively, which was 13.9- to 25.6-fold more potent than positive control.

关键词： 1-Anilino-5H-pyridazino[4,5-b]indole EGFR inhibitor Cytotoxicity

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THE SPECIALIZATION OF JUSTICE ORGANS AND PROFESSIONALIZATION OF JUSTICE PERSONNEL IN CHINESE JUVENILE CRIMINAL CASES

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Frontiers of Law in China-Selected Publications from Chinese Universities 2014年第3期9卷 403-424页

作者： LIAO Ming shi limei 1. College for Criminal Law Science Beijing Normal University Beijing China. This article is supported by “The Fundamental Research Funds for the Central Universities.”2. Law School Beijing Normal University Beijing China

Juvenile criminal cases should be investigated, prosecuted and judged by specialized organs and full-time personnel China should follow international criminal judicial criteria and implement the relevant laws, such as the Chinese Criminal Procedure Law and the Law on Protection of Minors in China, to promote the specialization of justice organs and the professionalization of justice personnel involved in juvenile criminal cases.

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Community structure of aerobic anoxygenic phototrophic bacteria in algae-and macrophyte-dominated areas in Taihu Lake,China

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Journal of Oceanology and Limnology 2022年第5期40卷 1855-1867页

作者： limei shi Yuanfeng CAI Xiaoli shi Min ZHANG Qingfei ZENG Fanxiang KONG Ping XU State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing 210008China The Philips Institute of Oral and Craniofacial Molecular Biology Virginia Commonwealth UniversityRichmondVA 23298-0566United States of America

Aerobic anoxygenic phototrophic bacteria(AAPB)repre sent a major group of bacterioplankton assemblages in many water systems and some are assumed to be closely associated with ***,studies on relationships between AAPB and cyanobacterial blooms are in *** dynamics of the abundance and diversity of AAPB was compared based on pufM gene in Meiliang Bay(featured by cyanobacterial blooms)and East Bay(featured by macrophyte)of Taihu Lake,a shallow subtropical lake in the East China *** abundance was not significantly different between the two sites,and they were positively correlated with dissolved organic carbon(DOC)*** ratios of AAPB to total bacteria varied from 3.4%to 11.5%and peaked in winter in both site *** significant differences of AAPB community compositions were detected between the two sites,but there was a separation between warm seasons(June,August,and October)and cold seasons(December,February,and April).Rhizobiales and Limnohabitans-like pufM sequences were significantly contributors for the difference between two seasons,and specially enriched in cold *** a(Ch1 a)and DOC were the most significant variables influencing the AAPB community ***,Porphyrobacter and Rhodospirillales-like pufM sequences were positively correlated with Ch1 a,indicating potential influence of cyanobacterial blooms on these AAPB *** results suggested that diverse AAPB ecotypes coexisted in Taihu Lake,and their ecological role in carbon cycling in the lake may not be ignored.

关键词： aerobic anoxygenic phototrophic bacteria cyanobacterial blooms dissolved organic carbon Alphaproteobacteria chlorophyll a

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Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria

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Journal of Environmental Sciences 2009年第11期21卷 1581-1590页

作者： shi limei CAI Yuanfeng YANG Hualin XING Peng LI Pengfu KONG Lingdong KONG Fanxiang State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Nanjing 210093 China State Key Laboratory of Lake Science and Environment Nanfing Institute of Geography and Limnology Chinese Academy of Sciences Nanfing 210008 China

Interactions between bacteria and cyanobacteria have been suggested to have a potential to influence harmful algal bloom dynamics; however, little information on these interactions has been reported. In this study, the bacterial communities associated with five strains of Microcystis aeruginosa, three species of other Microcystis spp., and four representative species of non-Microcystis cyanobacteria were compared. Bacterial 16S rDNA fragments were amplified and separated by denaturing gradient gel electrophoresis （DGGE） followed by DNA sequence analysis. The similarities among bacterial communities associated with these cyanobacteria were compared to the digitized DGGE profiles using the cluster analyses. The bacterial community structure of all cyanobacterial cultures differed. Cluster analysis showed that the similarity values among M. aeruginosa cultures were higher than those of other cyanobacterial cultures. Sequence analysis of DGGE fragments indicated the presence of bacteria including, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes and Actinobacteria in the cyanobacterial cultures. Members of the Sphingomonadales were the prevalent group among the Microcystis-associated bacteria. The results provided further evidence for species-specific associations between cyanoabcteria and heterotrophic bacteria, which are useful for understanding interactions between Microcystis and their associated bacteria.

关键词： bacterial diversity specificity Microcystis denaturing gradient gel electrophoresis

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Stocks and dynamics of particulate and dissolved organic matter in a large, shallow eutrophic lake(Taihu, China) with dense cyanobacterial blooms

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Journal of Oceanology and Limnology 2018年第3期36卷 738-749页

作者： shi limei HUANG Yaxin LU Yaping CHEN Feizhou ZHANG Min YU Yang KONG Fanxiang I State Key Laboratory of Lake Science and Environment Nanjing Institute of Geography and LimnologyChinese Academy of SciencesNanjing 210008China Biological Experiment Teaching Center College of Life SciencesNanjing Agricultural UniversityNanjing 210095China

Cyanobacterial blooms occur in eutrophic lakes worldwide, and greatly impair these ecosystems. To explore influences of cyanobacterial blooms on dynamics of both particulate organic matter(POM) and dissolved organic matter(DOM), which are at the base of the food chain, an investigation was conducted from December 2014 to November 2015 that included various stages of the seasonal cyanobacterial blooms(dominated by M icrocystis) in a large-shallow eutrophic Chinese lake(Taihu Lake). Data from eight sites of the lake are compiled into a representative seasonal cycle to assess general patterns of POM and DOM dynamics. Compared to December, 5-fold and 3.5-fold increases were observed in July for particulate organic carbon(POC, 3.05–15.37 mg/L) and dissolved organic carbon(DOC, 5.48–19.25 mg/L), respectively, with chlorophyll a(Chl a) concentrations varying from 8.2 to 97.7 μg/L. Approximately 40% to 76% of total organic carbon was partitioned into DOC. All C, N, and P in POM and DOC were significantly correlated with Chl a. POC:Chl a ratios were low, whereas proportions of the estimated phytoplankton-derived organic matter in total POM were high during bloom seasons. These results suggested that contributions of cyanobacterial blooms to POM and DOC varied seasonally. Seasonal average C:P ratios in POM and DOM varied from 79 to 187 and 299 to 2 175, respectively. Both peaked in July and then sharply decreased. Redundancy analysis revealed that Chl a explained most of the variations of C:N:P ratios in POM, whereas temperature was the most explanatory factor for DOM. These findings suggest that dense cyanobacterial blooms caused both C-rich POM and DOM, thereby providing clues for understanding their influence on ecosystems.

关键词： stoichiometry phytoplankton blooms eutrophic lake

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论刑事诉讼的多元治理范式

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政治与法律 2022年第12期 95-112页

作者：史立梅北京师范大学刑事法律科学研究院北京100875

随着我国刑法积极参与社会治理功能的日益强化以及犯罪圈的不断扩张,我国传统的以国家与被追诉人关系为主线、以惩罚犯罪为目的的刑事诉讼二元惩罚范式已经难以应对实体法变化带来的多重挑战。为了有效抑制刑法扩张,实现刑事司法的社会... 详细信息

随着我国刑法积极参与社会治理功能的日益强化以及犯罪圈的不断扩张,我国传统的以国家与被追诉人关系为主线、以惩罚犯罪为目的的刑事诉讼二元惩罚范式已经难以应对实体法变化带来的多重挑战。为了有效抑制刑法扩张,实现刑事司法的社会治理功能,刑事诉讼应从依赖国家专门机关自上而下对犯罪的打击和管控走向多元化的治理方式。刑事诉讼多元治理范式从治理理念出发,主张发挥国家专门机关对刑事诉讼的主导作用,尊重和保障当事人的正当程序权利,在查明案件事实真相的基础上,依靠被追诉人、被害人、社会力量的多方参与,化解矛盾、解决纠纷、实现对犯罪的修复与矫正。刑事诉讼多元治理范式在我国已经有相应的实践基础,但欲使其替代二元惩罚范式并充分发挥其犯罪治理作用,尚需进行一系列程序、制度和机制的调整。这具体包括拓宽程序出罪渠道、建立司法转处制度、打破诉讼期限的桎梏、完善权力约束机制以及确立重、轻罪案件的双轨制绩效考核机制等等。

关键词：刑事诉讼二元惩罚范式多元治理范式程序出罪司法转处

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论醉驾案件的程序出罪

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中国法学 2022年第4期 250-269页

作者：史立梅北京师范大学刑事法律科学研究院

醉驾入刑带来了我国犯罪结构和刑罚分布的明显变化,同时也带来了较为严重的刑罚溢出效应问题。针对醉驾入罪入刑过度问题,程序出罪较之其他出罪路径更能获得普遍认同。目前我国轻微刑事案件的程序出罪体现于公安机关的立案环节和检察机... 详细信息

醉驾入刑带来了我国犯罪结构和刑罚分布的明显变化,同时也带来了较为严重的刑罚溢出效应问题。针对醉驾入罪入刑过度问题,程序出罪较之其他出罪路径更能获得普遍认同。目前我国轻微刑事案件的程序出罪体现于公安机关的立案环节和检察机关的不起诉环节,但存在较多问题。欲充分发挥程序出罪对醉驾治理的功能,需要从程序法理论上予以反思。针对以醉驾为主的轻微犯罪,我国有必要强化司法程序的出罪功能,使刑事诉讼从原有的圆柱状结构向漏斗型结构转化。需罚性理论可以为醉驾案件程序出罪提供相应的依据并框定程序出罪的基本逻辑。未来我国醉驾案件的程序出罪路径可以从区分醉驾案件的实体出罪和程序出罪、完善现有的程序出罪路径、挖掘潜在的程序出罪路径等方面加以完善。

关键词：醉驾案件程序出罪诉讼结构需罚性特殊预防

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图书馆之城的魅力——以深圳市罗湖区公共图书馆建设为例

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图书馆建设 2009年第7期 41-46页

作者：师丽梅深圳市罗湖区图书馆广东深圳518021

公共图书馆以向社会提供信息资源和信息服务的方式成为政府为公民实现文化权利的保障机构之一。在政府的高度重视下,深圳图书馆之城建设取得了显著成就,在深圳公共文化服务体系中发挥了巨大的作用。从罗湖区公共图书馆5年建设、积累、... 详细信息

公共图书馆以向社会提供信息资源和信息服务的方式成为政府为公民实现文化权利的保障机构之一。在政府的高度重视下,深圳图书馆之城建设取得了显著成就,在深圳公共文化服务体系中发挥了巨大的作用。从罗湖区公共图书馆5年建设、积累、成长的发展及硕果可以窥见深圳图书馆之城的魅力及值得深思与借鉴的内容。

关键词：公共图书馆总分馆制图书馆服务阅读推广

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逮捕必要性条件的法释义学分析

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法学杂志 2019年第3期40卷 67-75页

作者：史立梅北京师范大学刑事法律科学研究院北京100875

作为彰显审查逮捕职能独立品格的逮捕必要性条件,是区分审查逮捕与侦查、审查起诉职能的关键因素。我国刑事诉讼法有关逮捕必要性条件的规定,内在地包含着社会危险性条件和逮捕之不可替代性条件的双层逻辑结构,前者决定逮捕理由的正当性... 详细信息

作为彰显审查逮捕职能独立品格的逮捕必要性条件,是区分审查逮捕与侦查、审查起诉职能的关键因素。我国刑事诉讼法有关逮捕必要性条件的规定,内在地包含着社会危险性条件和逮捕之不可替代性条件的双层逻辑结构,前者决定逮捕理由的正当性,后者决定逮捕手段的正当性。对逮捕社会危险性条件的审查判断,需运用证明和综合评估两种方法。对逮捕之不可替代性条件的判断并非社会危险性评估的附带结果,由专门的组织或者人员在社会危险性评估的基础上制定个别化的监管方案并向审查主体提出逮捕或者释放的建议,有助于解决审查主体时间、精力不足的问题,并能有效避免判断结果的主观随意性。

关键词：逮捕必要性社会危险性逮捕之不可替代性风险评估风险防控

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