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Impact of ocean acidification on physiology and microbiota in hepatopancreas of Pacific oyster Crassostrea gigas

Journal of Oceanology and Limnology 2022年第2期40卷 620-633页

作者： Lingshuai ZHANG Xiudan WANG Weiqian ZHANG Xiaoting YIN Qing LIU limei qiu CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of OceanologyCenter for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China Qingdao University of Science and Technology Qingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The hepatopancreas is an important tissue involved in various biological metabolism for mollusks,but its responses to ocean acidifi cation(OA)have not been well *** this study,the oysters were cultured in simulated conditions by continuously bubbling with ambient air(pH=8.10)or air-CO_(2)(pH=7.50)for up to two months,and the variations on the antioxidant capacity,digestive ability,and microbiota composition in hepatopancreas of Crassostrea gigas were *** results show that although superoxide dismutase and glutathione responded quickly to OA stress,the antioxidant capacity of the hepatopancreas was inhibited,as revealed by the decrease of the total antioxidant capacity,which led to an upward trend of the malondialdehyde,demonstrating that the oxidative damages were accumulated under the OA *** determination of the digestive ability manifested as the decrease of pepsin activity and the recovery of lipase and amylase activity after long-term acidifi cation,which may be helpful to improve the adaptability of *** addition,analysis on 16S rDNA amplicon revealed that the total species abundance and diversity of the hepatopancreas microbiota experienced a dynamic change,but fi nally it decreased greatly after long-term acidifi *** structure of the hepatopancreas microbiota was changed drastically with the change of the dominant species from aerobic to the anaerobic and facultative anaerobic bacteria,and the abnormal proliferation of some species,such as genus of Mycoplasma and order Clostridiales,which may aggravate the adverse eff ects of OA on the physiological functions of the *** a result,our fi ndings enrich our understanding of the accumulated oxidative damage and adaptive digestive ability in oyster hepatopancreas caused by *** the fi rst time,the changes of the hepatopancreas microbiota under long-term acidifi cation conditions are described,proving a good reference for the study of the response and adaptation

关键词： ocean acidifi cation oyster hepatopancreas antioxidant defense digestive enzymes microbiota

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Variations in nano-and pico-eukaryotic phytoplankton assemblages in the Qinhuangdao green-tide area

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Journal of Oceanology and Limnology 2022年第6期40卷 2446-2461页

作者： Weiqian ZHANG Hongbin HAN limei qiu Chao LIU Qingchun ZHANG Guizhong ZHOU Qingdao University of Science and Technology Qingdao 266071China CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of OceanologyCAS Center for Ocean Mega-ScienceChinese Academy of SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Ecology and Environmental Science Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Key Laboratory of Marine Eco-Environmental Science and Technology First Institute of OceanographyMinistry of Natural ResourcesQingdao 266061China University of Chinese Academy of Sciences Beijing 100049China Center of Ocean Mega Science Chinese Academy of SciencesQingdao 266071China

Qinhuangdao coastal waters have been frequently hitting by nano-and pico-eukaryotic phytoplankton(NPEP)blooms and green tides(macroalgal blooms)in the recent ***,understanding about the impacts of environmental factors and the green tides on the NPEP assemblages in this area is *** this study,the composition of NPEP assemblages and their variations were analyzed via amplicon sequence variants(ASVs)assay based on amplicon high-throughput sequencing data with the 18S V4 region as a targeted gene in the Qinhuangdao green-tide area during the green ***,average NPEP effective sequences and ASVs of 178000 and 200 were obtained from each sample,*** there were 25 classes,110 genera,and 97 species of NPEP were identified and annotated,the proportions of annotated ASVs at genus and species levels were only 44.7%and 17.8%,*** NPEP communities had a seasonal succession from diatom-dominated to *** the three investigations,Skeletonema,Karlodinium,and Gonyaulax were the most dominant genera in May,August,and September,*** diversity and the abundance of NPEP communities could be increased by a high content of dissolved organic nitrogen(DON)and dissolved organic phosphorus(DOP)but inhibited by low dissolved inorganic phosphorus *** outbreak of green tides could alter the composition and content of nutrients and accelerate the succession of the NPEP communities from diatom-dominated to dinoflagellate-dominated under the background of a seasonal increase in seawater *** results preliminarily revealed the impacts of the recurrent occurrences of green tides on the NPEP assemblages in the Qinhuangdao green-tide area exhibiting high DON content and dissolved inorganic nitrogen/phosphorus ratio.

关键词： nano-and pico-eukaryotic phytoplankton high-throughput sequencing green tide eutrophication Qinhuangdao

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Spectral and Morphological Analysis of Graphene-Based Two- Dimensional Carbon Materials

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China Petroleum Processing & Petrochemical Technology 2023年第3期25卷 12-21页

作者： Diao Yuxia Xiang Yanjuan Xin Mudi He Wenhui Xu Guangtong qiu limei Sinopec Research Institute of Petroleum Processing Co. Ltd.Beijing 100083China

This paper describes the spectral and morphological analysis of graphene, N-doped graphene, and graphenemodified with functional groups. The similarities and differences in the surface and microstructure are characterizedby infrared spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, scanning electron microscopy, andtransmission electron microscopy. Compared with high-purity graphene, the introduction of functional groups leads to moredefects in the two-dimensional structure. The quality of graphene, reflected by the intensity ratio of peak D and G modesin the Raman spectroscopy, is consistent with that observed by scanning electron microscopy and transmission electronmicroscopy. The infrared spectra of graphene-based two-dimensional carbon materials are different from that of high-puritygraphene, and the absorption peaks of the functional groups are obvious. The X-ray photoelectron spectroscopy resultsillustrate the diverse chemical states of carbon, and the atomic ratio of carbon to oxygen directly reflects the quality ofthe graphene-based materials. The results of electron microscopy and spectroscopic characterization of graphene samplesprovide an excellent basis for a wide range of applications in graphene production and quality control.

关键词： graphene carbon material Raman FTIR XPS TEM

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Identification of Ni_xS_y on Industrial Spent S Zorb Sorbents by Using XPS and TPR-MS

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China Petroleum Processing & Petrochemical Technology 2018年第3期20卷 42-47页

作者： Yuan Hui qiu limei Xu Guangtong Xin Mudi Shi Yanqiang Chen Shuai Zou Kang SINOPEC Research Institute of Petroleum Processing State Key Laboratory of Catalytic Materials and Reaction Engineering

Three industrial spent S Zorb sorbents extracted from production line were studied with XRD, TPR-MS and XPS. The characterization results of XPS and TPR-MS identified the existence of amorphous Ni_xS_y on industrial spent S Zorb sorbents, while the existing XRD quantitative analysis methods can only provide the long-range order in phase information and the grain size of Ni metal. XPS is a powerful tool to investigate the chemical states of nickel atom and the depthwise distribution of nickel species on S Zorb sorbent. Ni_xS_y and Ni metal species coexist on the industrial spent sorbents, and their percentages to total nickel slightly change with the operating conditions in the surface layer. It proves that Ni_xS_y is a stable intermediate product rather than a transition state. The information can contribute to the better elucidation of S Zorb desulfurization mechanism and offer a new direction for selectivity optimization of industrial S Zorb sorbents.

关键词： S Zorb sorbent nickel sulfide reaction mechanism X-ray photoelectron spectroscopy

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关于第二轮省志交通志篇目设置有关问题的探讨

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中国地方志 2007年第2期 12-14页

作者：邱丽梅安徽省公路管理局

篇目是志书的蓝图,在某种意义上决定着志书编修的成败。本文着重对第二轮省志交通志篇目设置涉及到的“大交通”与“小交通”、继承与创新、层次与结构、分与合、详与略等有关问题进行探讨,以期在第二轮志书编修中取得事半功倍的效果。

关键词：篇目问题探讨

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基于构效关系研究的石油炼制与化工催化剂表征技术进展

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石油学报（石油加工） 2024年第4期40卷 841-861页

作者：徐广通刘超文邱丽美中石化石油化工科学研究院有限公司石油化工分子转化与反应工程全国重点实验室北京100083

新型催化剂的研发模式正从过去以实验设计、试错为主向理论设计指导转变,基于催化剂组成结构信息与反应性能建立的构效关系是催化剂设计的重要基础。多种超高分辨电子显微技术、表面分析技术、核磁共振技术、光谱表征技术、基于同步辐... 详细信息

新型催化剂的研发模式正从过去以实验设计、试错为主向理论设计指导转变,基于催化剂组成结构信息与反应性能建立的构效关系是催化剂设计的重要基础。多种超高分辨电子显微技术、表面分析技术、核磁共振技术、光谱表征技术、基于同步辐射光源的高分辨谱学等技术为研究催化剂活性中心本质提供了重要手段。概述了近20年来催化剂表征技术的发展概况及构效关系的研究方法,重点介绍了原子尺度与原位表征技术的进展,以及表征技术在分子筛、加氢催化剂、重整和丙烷脱氢催化剂、脱硫吸附剂等典型催化剂研究中的应用情况,以期为研究人员更好地研究催化剂构效关系提供导引和启发。

关键词：催化剂原位表征构效关系石油炼制与化工

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X射线光电子能谱法研究加氢脱硫催化剂中活性元素的化学态

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石油学报（石油加工） 2011年第4期27卷 638-642页

作者：邱丽美齐和日玛刘清河徐广通中国石化石油化工科学研究院北京100083 北京理工大学化学物理研究所北京100081

利用S2p、Mo3d、W4f、Co2p_(3.2)和Ni2p_(3.2)X射线光电子能谱(XPS)和谱图解析方法分析了硫化态Co-Mo/Al_2O_3(SiO_2)和Ni-W/Al_2O_3加氢脱硫催化剂中各负载元素的常见化学态,简要阐述了XPS强度比法推测活性金属在载体表面分散状态的方... 详细信息

利用S2p、Mo3d、W4f、Co2p_(3.2)和Ni2p_(3.2)X射线光电子能谱(XPS)和谱图解析方法分析了硫化态Co-Mo/Al_2O_3(SiO_2)和Ni-W/Al_2O_3加氢脱硫催化剂中各负载元素的常见化学态,简要阐述了XPS强度比法推测活性金属在载体表面分散状态的方法。这些信息可为加氢脱硫催化剂工艺条件的选择、催化机理和失活原因的研究提供技术支撑。

关键词： XPS 硫化态加氢脱硫催化剂化学态分散度

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略论公路交通史编纂中的几个问题

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黄山学院学报 2007年第4期9卷 115-119页

作者：邱丽梅安徽省公路管理局史志编辑室安徽合肥230022

盛世编史,伴随着我国改革开放,中国大地上掀起了规模空前的公路交通史的编纂。如何正确认识并解决编纂过程中出现的一系列理论与实际问题,将直接影响史书编写的进度与质量。故着重对公路交通史的性质、研究对象、任务、研究范围进行分... 详细信息

盛世编史,伴随着我国改革开放,中国大地上掀起了规模空前的公路交通史的编纂。如何正确认识并解决编纂过程中出现的一系列理论与实际问题,将直接影响史书编写的进度与质量。故着重对公路交通史的性质、研究对象、任务、研究范围进行分析论述,以期编写出经得起时间考验,能切实起到为现实服务,推动时代前进的上乘之作。

关键词：公路交通史编纂问题

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高喷防渗墙技术在官棠泵站工程中的应用

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河南水利与南水北调 2023年第6期52卷 55-56页

作者：邱利梅佛山市禅城区骐升市政投资建设有限公司广东佛山528000

泵站工程是抽(排)水工程核心工程和水动力来源,发挥着合理分配和调控水资源的重要作用,泵站工程建设质量直接影响整个抽(排)水工程安全和经济效益。泵站工程建设基坑周边渗漏问题是水利泵站工程最大质量安全隐患,若泵站基坑围护渗漏问... 详细信息

泵站工程是抽(排)水工程核心工程和水动力来源,发挥着合理分配和调控水资源的重要作用,泵站工程建设质量直接影响整个抽(排)水工程安全和经济效益。泵站工程建设基坑周边渗漏问题是水利泵站工程最大质量安全隐患,若泵站基坑围护渗漏问题不及时解决,将会对整个泵站工程建设质量安全造成严重后果,不仅影响泵站工程整体建设质量,还可能威胁到周边居民生活安全。在泵站工程建设中使用高喷防渗墙技术,可有效强化泵站防渗效果,提升整体施工质量。文章立足高喷防渗墙技术施工要点,结合工程案例,对高喷防渗墙技术具体应用展开探讨,提出有效应用策略,以供参考。

关键词：水利工程泵站高喷防渗墙技术钻孔

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固废材料对混凝土性能的影响探讨

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江西建材 2021年第7期 21-22页

作者：丘丽妹上杭县建设工程质量安全监督站福建上杭364200

文中基于基准配合比,采用单因素方法,探讨粉煤灰、再生骨料及石粉等因素对水泥混凝土坍落度及抗压强度的影响,为固废材料在混凝土中的应用提供参考。

关键词：粉煤灰再生骨料石粉混凝土坍落度抗压强度

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