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Photochemical Degradation of the Ciprofloxacin Antibiotic and Its Microbiological Validation

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Journal of Environmental Science and Engineering(A) 2012年第4期1卷 448-453页

作者： Fernando Hernaindez Antonio Rivera Angeles Ojeda Teresa Zayas lilia.cedillo Environmental Chemistry Labs Chemical Center Sciences Institute BenJmerita Universidad Aut6noma de Puebla Puebla 72570Mexico Center for Mierobioloeical Sciences Research. Bendmerita Universidad Autonoma de Puebla. Puebla 72570. Mexico Chemical Engineering Faculty Benemerita Universidad A utonoma de Puebla Puebla 72570 Mexico

In this research, it used advanced oxidation processes for the recovery, detoxification and mineralization of wastewater mainly contaminated by antibiotics （ciprofloxacin）. These processes can be used alone or in combination with each other or by complementing traditional methods, even allowing the disinfection of bacterial and viral inactivation. With the use of experimental systems UV/H202/O3, UV/H202, it can achieve total mineralization of the compound. Ciprofloxacin solutions used at 50, 100, 200, 300, 400 and 500 ppm, degraded to 100% of antibiotics and by a microbiological method show that the loss of biological activity is inversely proportional to the time of irradiation.

关键词： Emerging contaminants advanced oxidation processes antibiotics photodegradation.

Adherence of a Clinical Strain of Mycoplasma fermentans to Human Cultured Epithelial Cells

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Advances in Microbiology 2014年第11期4卷 726-735页

作者： Mónica Rosales-Pérez Silvia Giono-Cerezo Jorge Girón Antonio Yánez lilia.cedillo Facultad de Agrobiología Universidad Autónoma de TlaxcalaMéxico Escuela Nacional de Ciencias Biológicas I.P.NMéxicoD.F.México Centro de Detección Biomolecular VI.E.P.B.U.A.P.PueblaPueMéxico Facultad de Estomatología B.U.A.P.PueblaMéxico Centro de Investigaciones en Ciencias Microbiológicas Instituto de CienciasB.U.A.P.PueblaPueMéxico

This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells;this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.

关键词： Mycoplasma fermentans Mycoplasmas Adherence Pathogenesis

Detection of Mycoplasmas in Patients with Amyotrophic Lateral Sclerosis

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Advances in Microbiology 2014年第11期4卷 712-719页

作者： Constantino Gil Alma Aurora Sánchez González Isidro Lecona León Antonio Rivera Rayo Santellán Olea lilia.cedillo Centro de Investigaciones en Ciencias Microbiológicas Instituto de Ciencias Benemérita Universidad Autónoma de Puebla Edificio 103J Ciudad Universitaria Col. San Manuel Puebla Pue México Escuela de Biología Benemérita Universidad Autónoma de Puebla Edificio 112A Ciudad Universitaria Col. San Manuel Puebla Pue México Servicio de Neurología Centro Médico Nacional Manuel ávila Camacho IMSS Puebla México

Amyotrophic Lateral Sclerosis (ALS) is a progressive degenerative disease of the motor neurons and the cause is unknown. Diverse factors such as genetic defects, nutritional deficiencies, head trauma, environmental toxin, autoimmune responses and viral and bacterial infections are involved. Mycoplasmas have been implicated as causal agents of different illnesses in human. The purpose of this study was to investigate the presence of mycoplasmas in the bloodstream of patients with ALS. Patients with ALS and healthy individuals were included in the study. A blood sample was taken in tubes with or without anticoagulant. Mycoplasmas were detected by culture or direct PCR, and the presence of antibodies IgM and IgG against LAMPs of these microorganisms by Western blot. Cultures for aerobic facultative bacteria were also done. Blood samples from 13 patients and 44 healthy individuals were screened. All blood cultures for non-fermentative mycoplasmas and aerobic facultative bacteria were negative. Cultures for fermentative mycoplasmas were considered positive after identification of mycoplasmal DNA by PCR. Mycoplasma sp. was detected by culture or direct PCR in 6/13 (46%) patients and in 4/44 (9%) of healthy individuals. M. fermentans was detected by PCR using specific primers in six patients and in two healthy individuals. IgM against LAMPs of M. fermentans were detected in 6/13 (46%) blood samples from patients and in 13/44 (30%) from healthy individuals, while. IgG was detected in 4/13 (31%) patients and in 3/44 (7%) healthy individuals. The results of this study show that mycoplasmas cause a systemic infection and could play a role in the origin or progression of the ALS.

关键词： Amyotrophic Lateral Sclerosis Mycoplasmas Immune Response

Presence of Mycoplasma *** Patients with Asthma or Allergic Rhinitis

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Advances in Microbiology 2014年第11期4卷 720-725页

作者： Munoz-Zurita Guillermo Paz Martínez David Yánez Santos Jorge Antonio Gil Juárez Constantino cedillo Ramírez María lilia Alumno de Doctorado del Posgrado en Ciencias Ambientales Benemérita Universidad Autónoma de PueblaPueblaMexico Hospital Universitario de Puebla Benemérita Universidad Autónoma de PueblaPueblaMexico Facultad de Estomatología Benemérita Universidad Autónoma de PueblaPueblaMexico Centro de Investigaciones en Ciencias Microbiológicas Benemérita Universidad Autónoma de PueblaPueblaMexico Centro de Detección Biomolecular VIEPBenemérita Universidad Autónoma de PueblaPueblaMexico

Objective: To compare the presence of Mycoplasma spp. in pharyngeal swabs of patients with asthma, allergic rhinitis and controls, also to compare the reactivity to allergens in the three groups. Material and Methods: Throat swabs of 120 patients with asthma, 211 allergic rhinitis patients and 171 healthy persons were cultured in E media. Positive cultures were identified by PCR. Susceptibility of patients positive for isolation of Mycoplasma spp. to indoor, forest and undergrowth allergens were determined. Results: Mycoplasma spp. was isolated in 26.6% of samples from asthmatic patients, 36% in patients with allergic rhinitis and 2.9% in the group of healthy persons. Positive patients for Mycoplasma with asthma and allergic rhinitis showed susceptibility tomite, grass and thunder mainly. Conclusion: There is a higher incidence of Mycoplasma spp. in asthmatic and allergic rhinitis patients than healthy persons. Only patients with asthma or allergic rhinitis showed reactivity to environmental allergens.

关键词： Asthma Allergic Rhinitis Allergens Mycoplasma Spp.