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Traceless photodegradable polymer cocoons for universal protein delivery and light-controlled gene editing

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Science China Chemistry 2024年第2期67卷 664-676页

作者： Huaibin Yu Meron Tsegay Kifle lili chen Feihong Xiong Yan Zhang Shangbang Gao Wei Hou Shuliang chen Weining Zhao Yuzhou Wu Hubei Engineering Research Center for Biomaterials and Medical Protective Materials Hubei Key Laboratory of Bioinorganic Chemistry&Materia MedicaKey Laboratory of Material Chemistry for Energy Conversion and StorageMinistry of EducationSchool of Chemistry and Chemical EngineeringHuazhong University of Science and Technology(HUST)Wuhan430074China Zhengzhou Research Institute Harbin Institute of TechnologyZhengzhou450000China College of Life Science and Technology Huazhong University of Science and Technology(HUST)Wuhan430074China Institute of Medical Virology School of Basic Medical SciencesWuhan UniversityWuhan430071China College of Pharmacy Shenzhen Technology UniversityShenzhen518118China

Polymer conjugation was found highly valuable in clinic to improve the bioavailability of protein ***,it is still a tremendous challenge to achieve a complete release of original proteins from the conjugated hybrid under external stimulus to recover active proteins in the targeted ***,we report a general light-controlled protein delivery methodology by weaving a photodegradable polymer cocoon around proteins,which could reliably protect them from degradation in the dark while efficiently releasing them under illumination without any residual *** surface charge of the polymer shell is easily tunable to facilitate efficient cell *** versatility of this strategy is demonstrated by the delivery of the Cas9/sg RNA complex that realized light-controlled gene editing both in vitro and in vivo,and such repertoire is of particular value in regard to minimizing the off-target toxicity of CRISPR-Cas9-based gene therapy.

关键词： photodegradable polymer-protein conjugate protein delivery light control gene editing

Bioceramic scaffolds with two-step internal/external modification of copper-containing polydopamine enhance antibacterial and alveolar bone regeneration capability

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2024年第1期25卷 65-82页

作者： Xiaojian JIANG Lihong LEI Weilian SUN Yingming WEI Jiayin HAN Shuaiqi ZHONG Xianyan YANG Zhongru GOU lili chen Department of Oral Medicine the Second Affiliated HospitalZhejiang University School of MedicineHangzhou310009China Bio-nanomaterials and Regenerative Medicine Research Division Zhejiang–California International Nanosystem InstituteZhejiang UniversityHangzhou310058China

Magnesium-doped calcium silicate(CS)bioceramic scaffolds have unique advantages in mandibular defect repair;however,they lack antibacterial properties to cope with the complex oral ***,for the first time,the CS scaffold was functionally modified with a novel copper-containing polydopamine(PDA(Cu^(2+)))rapid deposition method,to construct internally modified(*P),externally modified(@PDA),and dually modified(*P@PDA)*** morphology,degradation behavior,and mechanical properties of the obtained scaffolds were evaluated in *** results showed that the CS*P@PDA had a unique micro-/nano-structural surface and appreciable mechanical *** the prolonged immersion stage,the release of copper ions from the CS*P@PDA scaffolds was rapid in the early stage and exhibited long-term sustained *** in vitro evaluation revealed that the release behavior of copper ions ascribed an excellent antibacterial effect to the CS*P@PDA,while the scaffolds retained good cytocompatibility with improved osteogenesis and angiogenesis ***,the PDA(Cu^(2+))-modified scaffolds showed effective early bone regeneration in a critical-size rabbit mandibular defect ***,it was indicated that considerable antibacterial property along with the enhancement of alveolar bone regeneration can be imparted to the scaffold by the two-step PDA(Cu^(2+))modification,and the convenience and wide applicability of this technique make it a promising strategy to avoid bacterial infections on implants.

关键词： Copper-containing polydopamine Modification Antibacterial property Bone regeneration Angiogenesis Bioceramic scaffold

The osteoclastic activity in apical distal region of molar mesial roots affects orthodontic tooth movement and root resorption in rats

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International Journal of Oral Science 2024年第2期16卷 322-332页

作者： Wenhao Zheng Xiaofeng Lu Guangjin chen Yufeng Shen Xiaofei Huang Jinfeng Peng Jiajia Wang Ying Yin Wencheng Song Mengru Xie Shaoling Yu lili chen Department of Stomatology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina School of Stomatology Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration WuhanChina Department of Stomatology The First Affiliated HospitalSchool of MedicineShihezi UniversityShiheziChina

The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic ***,the sensitivity of existing detection techniques is not sufficient,and the criteria for evaluating optimal force have not been yet ***,by employing 3D finite element analysis methodology,we found that the apical distal region(A-D region)of mesial roots is particularly sensitive to orthodontic force in ***-resistant acidic phosphatase(TRAP)-positive osteoclasts began accumulating in the A-D region under the force of 40 grams(g),leading to alveolar bone resorption and tooth *** the force reached 80 g,TRAP-positive osteoclasts started appearing on the root surface in the A-D ***,micro-computed tomography revealed a significant root resorption at 80 ***,the A-D region was identified as a major contributor to whole root *** was determined that 40 g is the minimum effective force for tooth movement with minimal side effects according to the analysis of tooth movement,inclination,and *** findings suggest that the A-D region with its changes on the root surface is an important consideration and sensitive indicator when evaluating orthodontic forces for a rat ***,our investigations into this region would aid in offering valuable implications for preventing and minimizing root resorption during patients’orthodontic treatment.

关键词： movement roots offering

The OsBZR1-OsSPX1/2 module fine-tunes the growth-immunity trade-off in adaptation to phosphate availability in rice

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Molecular Plant 2024年第2期17卷 258-276页

作者： Yuqing He Yao Zhao Jitao Hu Lanlan Wang Linying Li Xueying Zhang Zhongjing Zhou lili chen Hua Wangl Jiaoyu Wang Gaojie Hong State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Key Laboratory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Virology and BiotechnologyZhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China Key Laboratory of Traceability for Agricultural Genetically Modified Organisms Ministry of Agriculture and Rural AffairsHangzhou 310021P.R.China College of Life Sciences Zhejiang Sci-Tech UniversityHangzhou 310018P.R.China State Key Laboratory for Managing Biotic and Chemical Threats to the Qualty and Safety of Agro-products Zhejang Academy of Agricultural SciencesHangzhou 310021P.R.China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products Key Labory of Biotechnology in Plant Protection of MOA of China and Zhejiang ProvinceInstitute of Plant Protection and MicrobiologyZhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China

The growth-promoting hormones brassinosteroids(BRs)and their key signaling component BZR1 play a vital role in balancing normal growth and defense ***,we discovered that BRs and OsBZR1 up-regulated sakuranetin accumulation and conferred basal defense against Magnaporthe oryzae infection under normal *** shortages,including phosphate(Pi)deficiency,potentially disrupt this growth-defense ***1 and OsSPX2 have been reported to sense Pi concentration and interact with the Pi signal mediator OsPHR2,thus regulating Pi starvation *** this study,we discovered that OsSPX1/2 interacts with OsBZR1 in both Pi-sufficient and Pi-deficient conditions,inhibit-ing BR-responsive *** Pi is sufficient,OsSPX1/2 is captured by OsPHR2,enabling most of OsBzR1 to promote plant growth and maintain basal *** response to Pi starvation,more OsSPX1/2 is released from OsPHR2 to inhibit OsBZR1 activity,resulting in slower ***,our study reveals that the OsBZR1-SPX1/2 module balances the plant growth-immunity trade-off in *** Y.,Zhao Y.,Hu J.,Wang L.,Li L.,Zhang X.,Zhou Z.,chen L.,Wang H.,Wang J.,and Hong G.(2024).The OsBZR1-OsSPX1/2 module fine-tunes the growth-immunity trade-off in adaptation to phosphate availability in esponse to Pi availability.

关键词： immunity maintain adaptation

Royal jelly modulates serum lipid metabolism and improves brain lipid profiles in natural aging mice

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Food Science and Human Wellness 2024年

作者： lili chen Li Zhao Gaowei Zhang Zhuozhen Li Huihui Ming Liangliang Qu Fangjian Ning Liping Luo College of Food and Health， Beijing Technology and Business University Key Laboratory of Geriatric Nutrition and Health Ministry of Education， Beijing Technology and Business University School of Life Sciences， Nanchang University School of Life Science， Jiangxi Science & Technology Normal University

Royal jelly (RJ) is rich in various nutrients with multiple health-promoting properties. This study aims to evaluate whether RJ can modulate related metabolite in the natural aging mice. Male C57BL/6J mice drank RJ solution daily for 9 months. Determination of serum lipids and pathological analysis exhibited that triglycerides (TG)， serum cholesterol (TC)， and low-density lipoprotein cholesterol (LDL-C) were decreased by 31.15%， 9.96%， and 22.58%， respectively， the morphology of brain nerve cells was also effectively recovered after RJ intervention. Lipidomic analysis showed that RJ could improve the levels of different kinds of lipids in aging mice brain， especially by increasing the content of antioxidant ether ester， and that the ratio of monounsaturated fatty acid (MUFA) to polyunsaturated fatty acid (PUFA) was up by 23.46%， which could alleviate oxidative stress. Moreover， the metabolism of glycerol phospholipids， glycerols， and fatty acids in aging mice could be regulated by RJ. RJ intervention can effectively improve lipid metabolism disorders caused by aging.

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中国高标准农田建设政策对粮食生产能力的影响及其机制

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资源科学 2024年第1期46卷 145-159页

作者：陈莉莉彭继权江西财经大学经济学院南昌330013

【目的】高标准基本农田建设是落实国家粮食安全战略的重要举措,而如何提升粮食生产能力则是高标准农田建设政策实现“藏粮于地”的关键所在。【方法】本文基于2005—2020年的中国31个省份面板数据,运用连续型双重差分法分析高标准农田... 详细信息

【目的】高标准基本农田建设是落实国家粮食安全战略的重要举措,而如何提升粮食生产能力则是高标准农田建设政策实现“藏粮于地”的关键所在。【方法】本文基于2005—2020年的中国31个省份面板数据,运用连续型双重差分法分析高标准农田建设政策对粮食生产能力的影响及其作用机制。【结果】(1)高标准农田建设政策能够显著提升粮食生产能力,对粮食单位面积产量有正向影响,稳健性检验也证实了研究结果的可靠性。(2)异质性分析表明,政策实施效果在粮食主产区更明显,在南方地区也表现较强,而在经济水平较高的东部地区却较弱。此外,相比玉米而言,政策对水稻和小麦生产能力提升作用更好。(3)作用机制检验表明,高标准农田建设政策通过促进农业机械化、土地流转以及耕地复种来提升粮食生产能力。【结论】建议加大粮食主产区的高标准农田建设力度,重视农田水利灌排等配套设施的建设,强化高标准农田的建后管护工作,不断增强粮食综合生产能力,强化粮食安全保障。

关键词：高标准农田粮食安全粮食生产能力粮食单产连续型DID 中国

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比较视域下的“虚实”范畴及其多向呈现

中国文学批评 2024年第3期 105-113,191页

作者：陈丽丽河南大学文学院开封475001

“虚实”在中西方美学史上皆有深远影响。中国传统文化中,“虚实”的审美内涵极具衍展性,渗透在内容、技法、风格意境等方面,形成了重实、尚虚、虚实相生三种审美指向;西方美学中,“虚实”与摹仿说、现象学、存在主义、虚构性等思想密... 详细信息

“虚实”在中西方美学史上皆有深远影响。中国传统文化中,“虚实”的审美内涵极具衍展性,渗透在内容、技法、风格意境等方面,形成了重实、尚虚、虚实相生三种审美指向;西方美学中,“虚实”与摹仿说、现象学、存在主义、虚构性等思想密切相关。“虚实”在中西方文艺创作中各具特色,与各自的哲学根源有关,中国哲学把“道”作为宇宙之本,“虚实”与“阴阳”“有无”等元范畴相通;西方哲学以“存在”为宇宙本体,更加追求主体与存在。

关键词：虚实中西美学多向性根源

Catalytic conversion of mixed polyolefins under mild atmospheric pressure

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The Innovation 2024年第2期5卷 98-106页

作者： Binzhi Zhao Hui Tan Jie Yang Xiaohui Zhang Zidi Yu Hanli Sun Jialiang Wei Xinyi Zhao Yufeng Zhang lili chen Dali Yang Jin Deng Yao Fu Zheng Huang Ning Jiao State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Beijing National Laboratory for Molecular Sciences College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China The Institute for Advanced Studies Wuhan UniversityWuhan 430072China Hefei National Laboratory for Physical Sciences at the Microscale CAS Key Laboratory of Urban Pollutant ConversionAnhui Province Key Laboratory of Biomass Clean EnergyiChEMUniversity of Science and Technology of ChinaHefei 230026China State Key Laboratory of Organometallic Chemistry Chinese Academy of SciencesShanghai 200032China

The chemical recycling of polyolefin presents a considerable challenge,especially as upcycling methods struggle with the reality that plastic wastes typically consist of mixtures of polyethylene(PE),polystyrene(PS),and polypropylene(PP).We report a catalytic aerobic oxidative approach for polyolefins upcycling with the corresponding carboxylic acids as the *** method encompasses three key ***,it operates under atmospheric pressure and mild conditions,using O_(2) or air as the ***,it is compatible with high-density polyethylene,low-density polyethylene,PS,PP,and their ***,it uses an economical and recoverable metal *** has been demonstrated that this approach can efficiently degrade mixed wastes of plastic bags,bottles,masks,and foam boxes.

关键词： mixed Catalytic oxidative

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联合循环发电站电力输出预测

发电技术 2024年第1期45卷 99-105页

作者：陈代俊陈里里李阳涛重庆交通大学机电与车辆工程学院重庆市南岸区400074

为了使联合循环发电站利润最大化,准确预测其满负载电力输出非常重要。联合循环发电站运行时,前一级产生的废气被用来驱动下一级热机,以此来推动发电机,其满负载电力输出受到环境温度、大气压强、相对湿度和废气气压的影响。为此,首先,... 详细信息

为了使联合循环发电站利润最大化,准确预测其满负载电力输出非常重要。联合循环发电站运行时,前一级产生的废气被用来驱动下一级热机,以此来推动发电机,其满负载电力输出受到环境温度、大气压强、相对湿度和废气气压的影响。为此,首先,采用核主成分分析(kernel principle component analysis,KPCA)对电站发电相关的特征进行特征组合降维完成特征提取;然后,采用极端梯度提升(extreme gradient boosting,XGBoost)算法进行特征重要性评分,并结合序列前向选择法(forward selection,FS)获取最优特征子集;最后,构建了KPCA-XGB-FS模型用于联合循环发电站满负载下小时电力输出预测。通过对某联合发电站的真实数据进行实验,并与使用相同数据的已有研究方法进行对比,结果表明,所提出方法能够有效对电力输出进行预测,预测结果优于已有的研究方法。

关键词：联合循环发电站电力输出特征提取核主成分分析(KPCA) 前向选择