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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期12卷 549-557页

作者： Yan Zheng Ei Ei Khine Khin Mar Thi Ei Ei Nyein likun huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Fujian Provincial Key Laboratory of Crop Breeding by Design Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Key Laboratory of Genetics Breeding and Multiple Utilization of CropsMinistry of EducationFujian Agriculture and Forestry UniversityFuzhou 350002FujianChina Department of Botany Mawlamyine UniversityMon StateMawlamyineMyanmar Department of Botany Dagon UniversityNorth DagonYangonMyanmar

Bulked-segregant analysis by deep sequencing(BSA-seq) is a widely used method for mapping QTL(quantitative trait loci) due to its simplicity, speed, cost-effectiveness, and efficiency. However, the ability of BSA-seq to detect QTL is often limited by inappropriate experimental designs, as evidenced by numerous practical studies. Most BSA-seq studies have utilized small to medium-sized populations, with F2populations being the most common choice. Nevertheless, theoretical studies have shown that using a large population with an appropriate pool size can significantly enhance the power and resolution of QTL detection in BSA-seq, with F_(3)populations offering notable advantages over F2populations. To provide an experimental demonstration, we tested the power of BSA-seq to identify QTL controlling days from sowing to heading(DTH) in a 7200-plant rice F_(3)population in two environments, with a pool size of approximately 500. Each experiment identified 34 QTL, an order of magnitude greater than reported in most BSA-seq experiments, of which 23 were detected in both experiments, with 17 of these located near41 previously reported QTL and eight cloned genes known to control DTH in rice. These results indicate that QTL mapping by BSA-seq in large F_(3)populations and multi-environment experiments can achieve high power, resolution, and reliability.

关键词： BSA-seq QTL mapping Large F3 population Multi-environment experiment Cross-validation

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可供性视角下的文化生产平台化:动因、机制与未来

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湖南大学学报（社会科学版） 2024年第1期38卷 146-151页

作者：王溥黄丽坤华中科技大学新闻与信息传播学院湖北武汉430074

作为整个社会平台化进程的一部分,文化生产平台化重新配置了文化内容的生产、传播与商品化,为文化实践与社会互动带来了新的机会与可能性。研究发现,技术可供性和想象可供性共同驱动了平台文化生产,影响了文化生产者的内容形态与创意表... 详细信息

作为整个社会平台化进程的一部分,文化生产平台化重新配置了文化内容的生产、传播与商品化,为文化实践与社会互动带来了新的机会与可能性。研究发现,技术可供性和想象可供性共同驱动了平台文化生产,影响了文化生产者的内容形态与创意表达。平台通过可见性机制设计和多面市场商业模式运作,得以支配文化生产和文化实践。值得注意的是,中国语境下的文化生产平台化嵌入特定历史阶段、政治经济制度与社会价值规范之中,也面临着不稳定性风险。在此过程中,机遇与挑战并存,共同塑造着当下文化生产者复杂的劳动体验与平台社会多元的文化景观。

关键词：平台化文化生产平台可供性

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Chemical characteristics and source apportionment of atmospheric particles during heating period in Harbin, China

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Journal of Environmental Sciences 2014年第12期26卷 2475-2483页

作者： likun huang Guangzhi Wang School of Food Engineering Harbin University of Commerce State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology

Atmospheric particles（total suspended particles（TSPs）; particulate matter（PM） with particle size below 10 μm, PM10; particulate matter with particle size below 2.5 μm, PM2.5）were collected and analyzed during heating and non-heating periods in Harbin. The sources of PM10 and PM2.5were identified by the chemical mass balance（CMB） receptor *** indicated that PM2.5/TSP was the most prevalent and PM2.5was the main component of PM210, while the presence of PM10–100was relatively weak. SO-4and NO-3concentrations were more significant than other ions during the heating period. As compared with the non-heating period, Mn, Ni, Pb, S, Si, Ti, Zn, As, Ba, Cd, Cr, Fe and K were relatively higher during the heating period. In particular, Mn, Ni, S, Si, Ti, Zn and As in PM2.5were obviously higher during the heating period. Organic carbon（OC） in the heating period was 2–5 times higher than in the non-heating period. Elemental carbon（EC） did not change much. OC/EC ratios were 8–11 during the heating period, which was much higher than in other Chinese cities（OC/EC： 4–6）. Results from the CMB indicated that 11 pollution sources were identified, of which traffic, coal combustion, secondary sulfate, secondary nitrate, and secondary organic carbon made the greatest contribution. Before the heating period, dust and petrochemical industry made a larger contribution. In the heating period, coal combustion and secondary sulfate were higher. After the heating period, dust and petrochemical industry were higher. Some hazardous components in PM2.5were higher than in PM10, because PM2.5has a higher ability to absorb toxic substances. Thus PM2.5pollution is more significant regarding human health effects in the heating period.

关键词： Atmospheric particles Chemical characteristics Heating period Chemical mass balance

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LC-MS/MS法测定鲤鱼中苦参碱和氧化苦参碱

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环境化学 2024年第6期43卷 2149-2152页

作者：高磊黄丽坤姜冰吴岩周春卫王妍哈尔滨商业大学食品工程学院哈尔滨150028 哈尔滨海关技术中心哈尔滨150028 岛津企业管理(中国)有限公司沈阳110000

建立基于前处理技术结合液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对鲤鱼基质中苦参碱和氧化苦参碱残留量测定的分析方法.样品采用乙腈均质提取的萃取方式,用硫酸钠作为除水剂,样品经过C18色谱柱... 详细信息

建立基于前处理技术结合液相色谱-串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)对鲤鱼基质中苦参碱和氧化苦参碱残留量测定的分析方法.样品采用乙腈均质提取的萃取方式,用硫酸钠作为除水剂,样品经过C18色谱柱分离,以水-乙腈为流动相,基质匹配标准曲线外标法定量.苦参碱和氧化苦参碱在质量浓度范围1—50 ng·mL^(−1)内,具有良好的线性关系,相关系数R^(2)均大于0.99.苦参碱方法检出限为0.21 ng·mL^(−1),定量限为0.69 ng·mL^(−1);氧化苦参碱方法检出限为0.17 ng·mL^(−1),定量限为0.52 ng·mL^(−1).添加浓度水平1.0、2.0、5.0 ng·mL^(−1),平均回收率为72.5%—108.7%,相对标准偏差(relative standard deviation,RSD,n=6)为4.2%—8.2%.苦参碱和氧化苦参碱的基质效应均表现为抑制.该方法前处理方法简单,灵敏度高,稳定性好,适合鲤鱼基质苦参碱和氧化苦参碱残留量的测定,同时为后续同类样品检测提供参考,也为今后相关标准制定提供数据积累.

关键词：苦参碱氧化苦参碱液相色谱-串联质谱法(LC-MS/MS) 鲤鱼基质效应

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Chemical characteristics and source apportionment of PM_(10) during a brown haze episode in Harbin, China

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Particuology 2011年第1期9卷 32-38页

作者： likun huang Chung-Shin Yuan Guangzhi Wang Kun Wang School of Food Engineering Harbin University of Commerce Harbin 150076 China School of Municipal and Environmental Engineering Harbin Institute of Technology Harbin 150090 China State Key Laboratory of Urban Water Resources and Environments Harbin Institute of Technolngy Harbin 150090 China Institute of Environmental Engineering National Sun Yat-sen University Taiwan 80424 China

This study investigates the correlation between PM10 and meteorological factors such as wind speed, atmospheric visibility, dew point, relative humidity, and ambient temperature during a brown haze episode. In order to identify the potential sources of PMlo during brown haze episode, respirable par- ticulate matter （PM10） was collected during both non-haze days and haze days and further analyzed for metallic elements, ionic species, and carbonaceous contents. Among them, ionic species contributed 45-64% to PM10, while metallic elements contributed 7-21% to PM10 which was smaller than the other chemical constituents. The average OC/EC ratio （42） in haze days was about three times of the average OC/EC ratio （14） in non-haze days. By using chemical mass balance （CMB） receptor model, the major sources were apportioned, including traffics, incinerators, coal combustion, steel industry, petrochemical industry, and secondary aerosols, etc. The contribution to PM10 concentration of each source was calcu- lated for all the samples collected. The results showed that coal combustion was the major source of PM10 in non-haze days and secondary aerosols were the major source in haze days, followed by petrochemical industry, incinerators, and traffics, while other sources had negligible effect.

关键词： PM 10 Chemical analysis Meteorological factors CMB receptor model Source apportionment

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村域土地资源适宜性评价及其格局优化——以福建南靖金山镇十村为例

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住区 2024年第4期 131-138页

作者：廖红春罗涛刘江黄丽坤福州大学建筑与城乡规划学院福建省国土空间分析与模拟数字技术重点实验室

土地资源作为乡村发展的基础条件,在乡村振兴中扮演着重要角色。本文以福建省南靖县金山镇十村为例,运用MCR模型获得土地适宜性分区,并将其与现状土地利用性质对比,识别得到各类用地的冲突斑块。研究遵循生态功能保护优先、基本农田不... 详细信息

土地资源作为乡村发展的基础条件,在乡村振兴中扮演着重要角色。本文以福建省南靖县金山镇十村为例,运用MCR模型获得土地适宜性分区,并将其与现状土地利用性质对比,识别得到各类用地的冲突斑块。研究遵循生态功能保护优先、基本农田不减少、耕地总量保持平衡、集约节约以及高效利用的原则优化冲突斑块,并通过景观格局指数的计算验证了优化方案的科学性与合理性。研究结果表明:优化调整后,生态保护区用地面积同比增加112.3ha,生产用地和生活用地同比减少103.19ha和7.76ha,实现了生态保护、农业生产用地及建设用地更加集约节约等规划目标。这一方法将有助于促进土地资源的协调发展,实现村域土地资源的高效利用,助力乡村振兴。

关键词： MCR模型适宜性评价景观格局土地资源村域

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微机原理及应用课程实验思政融合

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高师理科学刊 2024年第2期44卷 83-87,91页

作者：黄自鑫黄丽坤卢俊杰徐丽武汉工程大学电气信息学院湖北武汉430205

思政课程和课程思政同向同行是高校专业课程的必要改革发展路径,是培养新工科人才的重要举措.以微机原理及应用课程实验为例,针对该课程实验在教学内容、教学平台和教学模式等方面存在的问题,构建“思政融入,学赛共轭”的教学体系.通过... 详细信息

思政课程和课程思政同向同行是高校专业课程的必要改革发展路径,是培养新工科人才的重要举措.以微机原理及应用课程实验为例,针对该课程实验在教学内容、教学平台和教学模式等方面存在的问题,构建“思政融入,学赛共轭”的教学体系.通过思政挖掘、校企合作和赛教融合三路径,对课程实验教学进行改革,旨在提升学生专业综合能力与思想政治素养.实践表明,该教学体系能够有效提高学生综合能力,达到课程思政目标和新工科人才培养需求.

关键词：微机原理及应用课程思政实验改革学赛共轭新工科

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土地开发强度与住宅能耗关联性的连续尺度研究——以浙江长兴县为例

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住区 2024年第2期 78-84页

作者：邱瑶罗涛张雪葳黄丽坤福州大学建筑与城乡规划学院福建省国土空间分析与模拟数字技术重点实验室

土地开发强度会影响住宅能耗已被广泛认知,而两者之间的关系是否受空间尺度制约尚不明确。本研究以浙江长兴县为例,以16栋住宅为核心划定200m~1800m不同缓冲半径,探究不同尺度下土地开发强度指标(如容积率、建筑密度、道路面积率和不透... 详细信息

土地开发强度会影响住宅能耗已被广泛认知,而两者之间的关系是否受空间尺度制约尚不明确。本研究以浙江长兴县为例,以16栋住宅为核心划定200m~1800m不同缓冲半径,探究不同尺度下土地开发强度指标(如容积率、建筑密度、道路面积率和不透水面率等)与住宅能耗的关联特征。结果表明:1.土地开发强度指标对住宅能耗往往具有正贡献,且反映在不同尺度。容积率在200m尺度,道路面积率在800m和1000m尺度,不透水面率在400m、800m~1400m和1800m尺度对住宅能耗有影响。三维空间紧凑度和土地开发强度综合评价指数在600m~1600m尺度对住宅能耗有影响。2.不同土地开发强度指标对于住宅能耗的敏感性存在差异,敏感性由大到小依次为:不透水面率>道路面积率>容积率>建筑密度;土地开发强度复合指标中,土地开发强度综合评价指数的敏感性与三维空间紧凑度相似,比二维无序开发度强。

关键词：低碳土地开发强度空间尺度住宅能耗

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E3 ubiquitin ligase PbrATL18 is a positive factor in pear resistance to drought and Colletotrichum fructicola infection

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Horticultural Plant Journal 2024年第3期10卷 698-712页

作者： likun Lin Qiming Chen Kaili Yuan Caihua Xing Qinghai Qiao Xiaosan huang Shaoling Zhang State Key Laboratory of Crop Genetics and Germplasm Enhancement/College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu210095China

The Arabidopsis Toxicos en Levadura(ATL)protein is a subfamily of the E3 ubiquitin ligases,which exists widely in plants and is extensively involved in plant growth and *** the ATL family has been identified in other species,such as Arabidopsis,Oryza sativa,and grapevine,few reports on pear ATL gene families have been *** this study,92 PbrATL genes were identified and analyzed from the Pyrus breschneideri *** analysis and phylogenetic tree generation divided them into nine subgroups,and chromosome localization analysis showed that the 92 PbrATL genes were distributed in 16 of 17 pear *** data and quantitative real-time polymerase chain reaction(qRT-PCR)experiments demonstrated that PbrATL18,PbrATL41,and PbrATL88 were involved in both pear drought resistance and Colletotrichum fructicola *** addition,Arabidopsis thaliana overexpressing PbrATL18 showed greater resistance to drought stress than the wild type(WT),and PbrATL18-silenced pear seedlings showed greater sensitivity to drought and *** infection than the ***18 regulated plant resistance by regulating chitinase(CHI),phenylalanine ammonia-lyase(PAL),polyphenol oxidase(PPO),catalase(CAT),peroxidase(POD),and superoxide dismutase(SOD)*** study provided a reference for further exploring the functions of the PbrATL gene in drought resistance and *** infection.

关键词： Pear Colletotrichum fructicola Arabidopsis Toxicos en Levadura(ATL) Drought stress

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Multi-environment BSA-seq using large F3 populations is able to achieve reliable QTL mapping with high power and resolution: An experimental demonstration in rice

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The Crop Journal 2024年第2期 549页

作者： Yan Zheng Ei Ei Khine Khin Mar Thi Ei Ei Nyein likun huang Lihui Lin Xiaofang Xie Min Htay Wai Lin Khin Than Oo Myat Myat Moe San San Aye Weiren Wu

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