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解淀粉芽孢杆菌wxy-b3产脂肽类物质对苹果斑点落叶病菌的抑菌活性研究

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陕西科技大学学报 2025年第1期43卷 46-51页

作者：崔皓玥田露胡乐梅龚国利陕西科技大学食品科学与工程学院陕西西安710021

苹果斑点落叶病菌是苹果生产过程中常见的病害,严重危害苹果农作物的产量.脂肽作为一种主要来源于微生物次生代谢产物的抗菌剂,具有广谱抑菌、低耐药性、安全低毒等优势.本研究发现,解淀粉芽孢杆菌wxy-b3产生的脂肽类物质能够抑制苹果... 详细信息

苹果斑点落叶病菌是苹果生产过程中常见的病害,严重危害苹果农作物的产量.脂肽作为一种主要来源于微生物次生代谢产物的抗菌剂,具有广谱抑菌、低耐药性、安全低毒等优势.本研究发现,解淀粉芽孢杆菌wxy-b3产生的脂肽类物质能够抑制苹果斑点落叶病菌的生长,对苹果斑点落叶病菌的最小抑菌浓度为16 mg/mL,此浓度可造成该病原菌细胞膜电位超极化、线粒体膜电位去极化、大分子蛋白质和核酸泄露,以及胞内活性氧和丙二醛含量分别累积增加0.33倍和5.44倍,并通过扫描电镜进一步观察到MIC处理组的细胞形态起皱、细胞膜破损.上述结果表明,脂肽可能通过破坏细胞膜系统,导致细胞形态发生变化,细胞膜破裂,改变细胞膜通透性来达到抑菌效果.该研究将为脂肽作为抑制苹果斑点落叶病菌的抗菌剂奠定理论基础.

关键词：解淀粉芽孢杆菌脂肽类物质苹果斑点落叶病菌抑菌活性

铸牢中华民族共同体意识研究回溯与展望(2017—2023)——基于CiteSpace的可视化分析

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三峡大学学报（人文社会科学版） 2025年第1期47卷 29-40,56页

作者：龚志祥谭雯心东北大学秦皇岛分校民族学学院河北秦皇岛066004

本文运用CiteSpace软件绘制可视化知识图谱,分析2017—2023年中国知网CNKI核心期刊(北大核心、CSSCI期刊)铸牢中华民族共同体意识研究领域的1 772篇高质量文献,从发文量及趋势、作者及机构、引用量及高频关键词、多学科跨学科及研究机... 详细信息

本文运用CiteSpace软件绘制可视化知识图谱,分析2017—2023年中国知网CNKI核心期刊(北大核心、CSSCI期刊)铸牢中华民族共同体意识研究领域的1 772篇高质量文献,从发文量及趋势、作者及机构、引用量及高频关键词、多学科跨学科及研究机构合作等进行分析。铸牢中华民族共同体意识研究的发文量自2017年始逐年增长,经过快速上升,达到高水平的平稳增长。铸牢中华民族共同体意识理论体系研究深化仍是热点,实践研究与政策响应研究需进一步拓展和深化。

关键词：铸牢中华民族共同体意识 CiteSpace可视化分析研究现状

Recent Advances in Chinese Medicine for Contrast-Induced Nephropathy

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Chinese Journal of Integrative Medicine 2018年第1期24卷 6-9页

作者： gong Xue-zhong department of nephrology shanghai municipal hospital of traditional chinese medicineshanghai university of traditional chinese medicine，shanghai 200071，China

Contrast-induced nephropathy （CIN）, also called contrast-induced acute kidney injury （CI-AKI）, is a leading cause of hospital-acquired AKI as a possible complication of intravenous contrast media administration.

关键词： Recent Advances in Chinese Medicine Contrast-Induced Nephropathy

Formation and Characterization of ZrO3 and HfO3 Molecules in Solid Argon

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Chinese Journal of Chemical Physics 2009年第2期22卷 113-118页

作者： Yu gong Ming-fei Zhou Department of Chemistry Shanghai Key Laboratory of Molecular Catalysts and Innovative Materials Advanced Materials Laboratory Fudan University Shanghai 200433 China

ZrO3 and HfO3 molecules were prepared via reactions of metal monoxides with dioxygen in solid argon and were characterized using matrix isolation infrared absorption spectroscopy as well as theoretical calculations. Unlike the titanium monoxide molecule, which reacted spontaneously with dioxygen to form TiO3, the ZrO and HfO molecules reacted with dioxygen to give the ZrO3 and HfO3 molecules only under visible light irradiation. Density functional calculations predicted that both the ZrO3 and HfO3 molecules possess a closed-shell singlet ground state with a non-planar C8 geometry, in which the side-on coordinated O2 falls into the peroxide category.

关键词： Matrix isolation Transition metal oxide Dioxygen complex Infrared spectrum

Profiling of N6-methyladenosine methylation in porcine longissimus dorsi muscle and unravelling the hub gene ADIPOQ promotes adipogenesis in an m^(6)A-YTHDF1–dependent manner

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Journal of Animal Science and Biotechnology 2023年第3期14卷 950-965页

作者： Huanfa gong Tao gong Youhua Liu Yizhen Wang Xinxia Wang Key Laboratory of Molecular Animal Nutrition Ministry of EducationCollege of Animal SciencesZhejiang UniversityHangzhou 310058People’s Republic of China Key Laboratory of Animal Nutrition and Feed Science in Eastern China Ministry of AgricultureCollege of Animal SciencesZhejiang UniversityHangzhou 310058People’s Republic of China

Background Intramuscular fat(IMF)content is a critical indicator of pork quality,and abnormal IMF is also relevant to human disease as well as *** N6-methyladenosine(m^(6)A)RNA modification was recently found to regulate adipogenesis in porcine intramuscular fat,however,the underlying molecular mechanisms was still *** In this work,we collected 20 longissimus dorsi muscle samples with high(average 3.95%)or low IMF content(average 1.22%)from a unique heterogenous swine population for m^(6)A sequencing(m^(6)A-seq).We discovered 70genes show both differential RNA expression and m^(6)A modification from high and low IMF group,including ADIPOQ and SFRP1,two hub genes inferred through gene co-expression ***,we observed ADIPOQ,which contains three m^(6)A modification sites within 3’untranslated and protein coding region,could promote porcine intramuscular preadipocyte differentiation in an m^(6)A-dependent ***,we found the YT521-B homology domain family protein 1(YTHDF1)could target and promote ADIPOQ mRNA *** Our study provided a comprehensive profiling of m^(6)A methylation in porcine longissimus dorsi muscle and characterized the involvement of m^(6)A epigenetic modification in the regulation of ADIPOQ mRNA on IMF deposition through an m^(6)A-YTHDF1-dependent manner.

关键词： ADIPOQ Intramuscular fat N6-methyladenosine Pig YTHDF1

Numerical investigation of granular mixing in an intensive mixer:Effect of process and structural parameters on mixing performance and power consumption

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Chinese Journal of Chemical Engineering 2021年第4期34卷 241-252页

作者： Zhijian Zuo Shuguang gong Guilan Xie School of Mechanical Engineering Xiangtan UniversityXiangtan 411105China

Discrete element method(DEM)simulations of particle mixing process in an intensive mixer were conducted to study the influence of structural and process parameters on the mixing performance and power *** DEM model was verified by comparing the impeller torque obtained from simulation with that from *** and vessel torque,coordination number(CN)and mixing index(Relative standard deviation)were adopted to qualify the particle dynamics and mixing performance with different parameters.A method based on cubic polynomial fitting was proposed to determine the critical mixing time and critical specific input work during the mixing *** is found that the mixing performance and energy efficiency increases with the decrease of impeller *** mixing performance is improved slightly with the increase of blade number and the impeller with 3 blades has the highest energy efficiency due to its low input *** indicate that the energy efficiency and the mixing performance increase with the decrease of filling level when the height of granular bed is higher than that of blade.

关键词： Intensive mixer Particle mixing DEM Mixing performance Power consumption

Layout graph model for semantic façade reconstruction using laser point clouds

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Geo-Spatial Information Science 2021年第3期24卷 403-421页

作者： Hongchao Fan Yuefeng Wang Jianya gong Department of Civil and Environmental Engineering Norwegian University of Science and TechnologyTrondheimNorway State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhanChina

Building façades can feature different patterns depending on the architectural style,function-ality,and size of the buildings;therefore,reconstructing these façades can be *** particular,when semantic façades are reconstructed from point cloud data,uneven point density and noise make it difficult to accurately determine the façade *** inves-tigating façade layouts,Gestalt principles can be applied to cluster visually similar floors and façade elements,allowing for a more intuitive interpretation of façade *** propose a novel model for describing façade structures,namely the layout graph model,which involves a compound graph with two structure *** the proposed model,similar façade elements such as windows are first grouped into clusters.A down-layout graph is then formed using this cluster as a node and by combining intra-and inter-cluster spacings as the ***,a top-layout graph is formed by clustering similar *** extracting relevant parameters from this model,we transform semantic façade reconstruction to an optimization strategy using simulated annealing coupled with Gibbs *** façade point cloud data with different features were selected from three datasets to verify the effectiveness of this *** experimental results show that the proposed method achieves an average accuracy of 86.35%.Owing to its flexibility,the proposed layout graph model can deal with different types of façades and qualities of point cloud data,enabling a more robust and accurate reconstruc-tion of façade models.

关键词： Building façade semantic reconstruction point cloud compound graph model stochastic process

DSSylation, a novel guide for protein degradation？

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Protein & Cell 2014年第2期5卷 91-93页

作者： Cheng-Xin gong Department of Neurochemistry Inge Grundke-lqbal Research Floor New York State Institute for Basic Research in Developmental Disabilities Staten Island NY 10314 USA

The natural design for the generation and the fate of proteins in an organism could be as complicated as life itself. A protein is biosynthesized strictly according to the message carried by the mRNA transcribed from its gene. However, when, where, what and how much a gene is expressed are regulated precisely and dynamically at multiple levels, including at transcription, mRNA maturation, alternative splicing, and translation levels. A newly synthesized protein often needs to be further modified and sorted posttransla- tionally for its optimal confirmation and function. When a protein reaches its life span, the senescent protein is degraded by protein degradation machinery involving the ubiquitin-proteosome system and lysosomes. Protein homeostasis is essential for maintaining the normal mor- phology and function of the cell.

关键词： protein degradation natural design biosynthesized strictly

Coking of Pt/γ-Al_(2)O_(3) catalyst in landfill gas deoxygen and its effects on catalytic performance

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Chinese Journal of Chemical Engineering 2023年第5期57卷 224-232页

作者： Xinyu Yang Zezhi Chen Huijuan gong State Key Laboratory of Pollution Control and Resource Reuse School of EnvironmentNanjing UniversityNanjing 210023China Center of Materials Analysis Nanjing UniversityNanjing 210093China

Catalytic oxidation of CH_(4) has been proved to be an attractive option for landfill gas(LFG) ***, coking of catalysts in catalytic LFG deoxygen has been clearly observed in industrial applications. In this regard, it is necessary to investigate whether coke deposition originates from CH_(4) or volatile organic compounds present in LFG, and the influence of coke deposition on catalytic performance. Herein,we evaluate the LFG deoxygen on Pt/γ-Al_(2)O_(3) catalyst in simulated LFG(CH_(4), CO_(2), O_(2), N_(2)) and its co-feed with representative volatile organic compounds, ethylbenzene, toluene, benzene and cyclohexane. The results show that the coking of the catalyst is originated from volatile organic compounds rather than CH_(4). The Pt/γ-Al_(2)O_(3) catalyst does not deactivate during LFG deoxygen process, even significant amount of coke deposited, up to 18.15%(mass). Characterization analyses reveal that although coke deposition overall covers the catalyst surface, resulting in mesopores blockage and a reduced number of accessible Pt sites, however, the coke formed, H-rich carbonaceous components, behaves as counterpart for O_(2) elimination. Besides, the coke deposited is mainly filamentous. Thus, coke formation has little negative effect on the overall catalytic performance of Pt/γ-Al_(2)O_(3) catalyst ultimately. The results obtained in this work are helpful for the rational design of robust Pt based catalysts for LFG deoxygen without undue attention to their coking properties, and also favor the innovation of more attractive purification scheme configurations.

关键词： Landfill gas Deoxygen Pt/γ-Al_(2)O_(3) Volatile organic compounds Coke deposition