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Comparative study of microbial community structure in different filter media of constructed wetland

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Journal of Environmental Sciences 2010年第1期22卷 127-133页

作者： Ming Li Qiaohong Zhou Min Tao Ying Wang lijuan jiang Zhenbin Wu State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Wuhan 430072 China Graduate University of Chinese Academy of Sciences Beijing 100039 China School of Resource & Environmental Engineering Wuhan University of Technology Wuhan 430070 China

Comparisons of microbial community structure, in eight filter media of zeolites, anthracite, shale, vermiculite, ceramic filter media, gravel, steel slag and bio-ceramic, were undertaken by analyzing the phospholipid fatty acid （PLFA） composition. A total of 20 fatty acids in the range of C18to C20 were determined but only 13 PLFAs were detected in steel slag. They consist of saturated fatty acids, branched fatty acids, monounsaturated fatty acids, and polyunsaturated fatty acids. The variation of fatty acids was revealed in the relative proportions of these fatty acids in different media. The aerobic prokaryotes were the predominant group in all media. The PLFA composition showed significant differences among the eight different media by Tukey＇s honestly test. It was found that steel slag was significantly different in the microbial community as compared to other filter media, probably due to its alkaline effluent. Steel slag alone is probably not a good choice of substratum in constructed wetlands. The principle components analysis （PCA） showed that zeolites, bio-ceramic, shale and vermiculite had a similar microbial community structure while steel slag and ceramic filter media were distinct from other media.

关键词： microbial community structure phospholipid filter media biomarkers constructed wetland steel slag

Bioinformatic analysis of embryo development related small heat shock protein Hsp26 in Artemia species

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Frontiers in Biology 2012年第4期7卷 350-358页

作者： Jiaqing WANG Lin HOU Zhenfeng HE Daizong Li lijuan jiang Science and Technology College Shenyang Agricultural University Fushun 113122 China College of Life Sciences Liaoning Normal University Dalian 116029 China State Key Laboratory of Supramolecular Structure and Materials Jilin University Changchun 130012 China College of Ocean Agriculture University of Hebei Qinhuangdao 066003 China

Artemia embryos can endure extreme temperature, long-term anoxia, desiccation and other wide variety of stressful conditions. How the embryos survive these stresses is a very interesting and unsolved subject. To solve this question we analyzed the nucleotide and deduced protein sequence for Hsp26, a molecular chaperone specific to Artemia embryo development, cDNAs of Hsp26 were sequenced from eight Artemia species and deduced Hsp26 amino acid sequences were analyzed. Computer-assisted analysis indicated that the 5＇-untranslated region and all the 3 introns contain many putative cis-acting elements for Hsp26 gene expression during development, including heat shock elements （HSEs）, Dfd, dl, CF2-II, Hb and AP-1 binding sites. Secondary structure of the Hsp26 3＇-untranslated terminator contains the basic structure basis for transcriptional termination. Hsp26 shares sequence similarity with sHSPs （small heat shock protein） from other organisms. The physico-chemical properties of the deduced protein, such as theoretical molecular weight, protein extinction coefficient, isoelectric point and antigenic sites were also obtained. One seven-peptide nuclear localization signals （NLS）＂PFRRRMM＂ was found, which suggested that the Hsp26 protein was hypothesized to be located inside the nucleus. The numbers of phosphorylation sites of serine, threonine and tyrosine and kinase specific phosphorylation sites are also located in Hsp26 protein sequence. These studies will help us achieve a better understanding of Hsp26 and broad implications for sHSPs function in crustacean embryo development.

关键词： bioinformatic analysis embryo development small heat shock protein Artemia species

Quantitative determination of multi-class bioactive constituents for quality control of Yiqi jiangzhi Granules

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Chinese Herbal Medicines 2022年第2期14卷 324-331页

作者： Shaobo Guo Shaowei Hu lijuan jiang Xiaohe Chen Wei Zhang Yanyan jiang Bin Liu School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing 102488China

Objective:To establish a reliable and sensitive method for evaluating quality of Yiqi jiangzhi Granules(YQJZG).Methods:Ultra performance liquid chromatography electrospray ionization tandem mass spectrometry(UPLC-ESI-MS/MS)was employed for simultaneous determination of eight marker *** was performed on an AQUITY UPLC;HSS T3 column,the mobile phase consisted of acetonitrile as the organic phase and 0.1%(volume percentage)formic acid as the *** marker components,ginsenoside Rg1(GRg1),ginsenoside Re(GRe),ginsenoside Rb1(Gb1),typhaneoside(TEO),isorhamnetin-3-O-neohespeidoside(IN),hesperidin(HPD),aurantio-obtusin-6-O-β-D-glucoside(AG)and curcumin(CCM),were detected by multiple reaction monitoring(MRM)*** Chinese Pharmacopoeia(2020 edition)was regarded as the guidance document for this method ***:The method showed good linearity(R^(2)≥0.9990).The relative standard deviation(RSD)values for the instrument precision,intermediate precision and repeatability were less than 2.91%,2.88%,and 3.54%,*** average recovery varied from 91.08%to 103.89%,with RSD below 3.81%.Sample solutions were found to be stable within 24 h at 4℃(RSD<2.85%).Eight marker components were successfully determined from three batches of ***:The proposed UPLC-ESI-MS/MS method was found to be simple,fast and sensitive,and can be used for the routine quality assessment of ***,this method may provide a new and powerful tool of quality control for other traditional Chinese medicine analogous formulae.

关键词： multiple reaction monitoring quality control traditional Chinese medicine UPLC-ESI-MS/MS Yiqi jiangzhi Granules

SABIC Strengthens Its Presence in China

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China Chemical Reporter 2008年第13期19卷 24-24页

作者： jiang lijuan

Saudi Basic Industries Corporation(SABIC)is further strengthening its leadership posi-tion in China,the world’s most importantand fastest growing polymers *** is the largest exhibitor

关键词： exhibitor fastest leadership today strengthening s

Effect of pH,fulvic acid and temperature on the sorption of uranyl on ZrP_2O_7

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Science China Chemistry 2010年第6期53卷 1429-1437页

作者： QIAN lijuan,HU PeiZhuo,jiang Zhenjiang,GENG YanXia & WU WangSuo Radiochemistry Laboratory,School of Nuclear Science and Technology,Lanzhou University,Lanzhou 73000,China 1. Radiochemistry Laboratory School of Nuclear Science and Technology Lanzhou University Lanzhou 73000 China

The sorption of UO22+ onto ZrP2O7 was studied using the batch technique and the point of zero charge of ZrP2O7 was obtained through mass *** results indicated that sorption of UO22+ onto ZrP2O7 was strongly affected by pH,solid-to-liquid ratio (m/V),the species of electrolyte in solution and fulvic acid (FA),but was insensitive to ionic *** sorption of UO22+ increased with increasing pH and m/*** presence of FA enhanced UO22+ sorption onto ZrP2O7 at low *** presence of phosphate or sulfate caused opposite effects on the sorption of UO22+ onto *** of citrate also significantly affected UO22+ *** sorption of UO22+ increased as the temperature of the system *** Langmuir and Freundlich models were used to simulate the sorption isotherms of UO22+ onto ZrP2O7 at different *** results indicated that the Freundlich model described UO22+ sorption better than the Langmuir *** parameters for the sorption process were calculated from the temperature dependent sorption *** results suggested that the sorption process of UO22+ onto ZrP2O7 is spontaneous and *** desorption process of UO22+ from ZrP2O7 was also investigated and it was found that sorption onto ZrP2O7 was irreversible.

关键词： ZrP2O7 uranyl sorption desorption thermodynamic data

Cryoablation techniques in bladder cancer: A review

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Frigid Zone Medicine 2024年第2期4卷 72-77页

作者： Binglei Ma Wilhem Teixeira lijuan jiang State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060China Department of Urology Sun Yat-sen University Cancer CenterGuangzhou 510060China

Bladder cancer(BC)ranks as the tenth most common cancer ***,BC is broadly categorized into urothelial and non-urothelial *** carcinoma represents over 90%of BC in most regions *** standard treatment procedure for diagnosing and treating non-muscle-invasive bladder cancer(NMIBC)is transurethral resection of bladder tumors(TURBT).Currently,the standard of care for muscle-invasive bladder cancer(MIBC)is neoadjuvant chemotherapy followed by radical *** therapy is a medical technique that uses extremely low temperatures to destroy diseased *** treatment serves as a therapeutic tool for both benign and malignant diseases in organs such as the kidney,prostate gland,lung,liver,and breast,and is particularly effective for unresectable tumors,offering less trauma,quick recovery,good tolerability,and symptom ***,cryoablation has its *** the past few years,cryoablation therapy has emerged as a new method for treating early *** treatment is minimally invasive,precise,and offers quick recovery,providing patients with a new treatment *** randomized studies are still limited,increasing evidence suggests its potential application in bladder cancer combined with transurethral resection(TURBT)or *** is not standard therapy for bladder *** decisions should be discussed by a multidisciplinary team of urologists,oncologists,and interventional physicians and require more randomized controlled trials to define patient selection criteria and treatment approaches.

关键词： bladder tumor transurethral resection of bladder tumors muscle-invasive bladder cancer non-muscle-invasive bladder cancer cryoablation

Efficacy and safety of repetitive transcranial magnetic stimulation for generalised anxiety disorder: A metaanalysis

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General Psychiatry 2019年第5期32卷 237-248页

作者： Huiru Cui lijuan jiang Yanyan Wei Wei Li Hui Li Junjuan Zhu Jiaoyan Pang Jijun Wang Chunbo Li Shanghai Key Laboratory of Psychotic Disorders Shanghai Mental Health CenterShanghai Jiao Tong University School of MedicineShanghaiChina School of government Shanghai University of Political Science and LawShanghaiChina Institute of Psychology and Behavioral Science Shanghai Jiao Tong UniversityShanghaiChina Center for Excellence in Brain Science and Intelligence Technology(CEBSIT) Chinese Academy of ScienceShanghaiChina Brain Science and Technology Research Center Shanghai Jiao Tong UniversityShanghaiChina

Background Pharmacological and conventional nonpharmacological treatments are only moderately effective in treatingeneralised anxiety disorder(GAD).Recently,repetitive transcranial magnetic stimulation(rTMS)has attracted interest because of its potential therapeutic *** To investigate the efficacy and safety of rTMS treatment for *** Literature studies published in English or Chinese were screened in 10 electronic databases up to 5 December *** included studies'bias risk was assessed using Cochrane risk of bias assessment ***-analysis was performed to compute the standardised mean difference(SMD)and risk ratio(RR)along with its 95%Cis through using RevMan *** was inspected by I2 and the χ2 *** performed subgroup analysis and meta-regression to investigate *** used funnel plot to assess publication *** used the GRADE approach to assess the whole quality of *** Twenty-one studies,with a total sample size of 1481,were *** risk of bias in most studies included is moderate,the majority of which are lacking of blinding methods of treatment *** treatment had beneficial effects in the rTMS group compared with the control group in mean anxiety score(SMD=-0.68;95%Cl-0.89 to-0.46).None of the 21 studies included here reported severe adverse *** for dropout rates,there are no statistically significant differences between the two groups(RR 1.14,95%Cl 0.72 to 1.82)or adverse events(RR 0.95,95%Cl 0.77 to 1.18).No particular influence on the heterogeneity of any variable was *** risk of publication bias was *** to the GRADE approach,the evidence levels of primary outcome(treatment effects)and secondary outcomes(acceptability and safety)were rated as‘medium’.Conclusion The use of rTMS combined with medication treatment may have a significant positive anti-anxiety effect on patients with ***,we should interpret the results cautiously due to the relatively hi

关键词： stimulation treatment magnetic

INCloud:integrated neuroimaging cloud for data collection,management,analysis and clinical translations

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General Psychiatry 2021年第6期34卷 58-65页

作者： Qingfeng Li lijuan jiang Kaini Qiao Yang Hu Bing Chen Xiaochen Zhang Yue Ding Zhi Yang Chunbo Li Shanghai Mental Health Center Shanghai Jiao Tong University School of MedicineShanghaiChina Jing Hengyi School of Education Hangzhou Normal UniversityHangzhouZhejiangChina Institute of Psychological and Behavioral Sciences Shanghai Jiao Tong UniversityShanghaiChina Brain Science and Technology Research Center Shanghai Jiao Tong UniversityShanghaiChina Center for Excellence in Brain Science and Intelligence Technology(CEBSIT) Chinese Academy of SciencesShanghaiChina

Background Neuroimaging techniques provide rich and accurate measures of brain structure and function,and have become one of the most popular methods in mental health and neuroscience *** growing neuroimaging research generates massive amounts of data,bringing new challenges in data collection,large-scale data management,efficient computing requirements and data mining and *** To tackle the challenges and promote the application of neuroimaging technology in clinical practice,we developed an integrated neuroimaging cloud(INCloud).INCloud provides a full-stack solution for the entire process of large-scale neuroimaging data collection,management,analysis and clinical *** INCloud consists of data acquisition systems,a data warehouse,automatic multimodal image quality check and processing systems,a brain feature library,a high-performance computing cluster and computer-aided diagnosis systems(CADS)for mental disorders.A unique design of INCloud is the brain feature library that converts the unit of data management from image to image features such as hippocampal *** the CADS to the scientific database,INCloud allows the accumulation of scientific data to continuously improve the accuracy of objective diagnosis of mental *** Users can manage and analyze neuroimaging data on INCloud,without the need to download them to the local *** users can query,manage,analyze and share image features based on customized *** examples of'mega-analyses'based on the brain feature library are *** Compared with traditional neuroimaging acquisition and analysis workflow,INCloud features safe and convenient data management and sharing,reduced technical requirements for researchers,high-efficiency computing and data mining,and straightforward translations to clinical *** design and implementation of the system are also applicable to imaging research platforms in other fields.

关键词： image diagnosis translation

Microbial diversity in lake sediments detected by PCR-DGGE

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Frontiers in Biology 2008年第3期3卷 293-299页

作者： Xinqing ZHAO Liuyan YANG Can CHEN Lin XIAO lijuan jiang Zhe MA Haowei ZHU Zhenyang YU Daqiang YIN State Key Laboratory of Pollution Control and Resource Reuse School of the EnvironmentNanjing UniversityNanjing 210093China

In this study,PCR-denaturing gradient gel electrophoresis(DGGE)was applied to analyze the microbial communities in lake sediments from Lake Xuanwu,Lake Mochou in Nanjing and Lake Taihu in *** samples from seven locations in three lakes were collected and their genomic DNAs were *** DNA yields of the sediments of Lake Xuanwu and Lake Mochou were high(10 mg/g),while that of sediments in Lake Taihu was relatively *** DNA purification,the 16S rDNA genes(V3 to V5 region)were amplified and the amplified DNA fragments were separated by parallel *** DGGE profiles showed that there were five common bands in all the lake sediment samples indicating that there were similarities among the populations of microorganisms in all the lake *** DGGE profiles of Lake Xuanwu and Lake Mochou were similar and about 20 types of micro-organisms were identified in the sediment samples of both *** results suggest that the sediment samples of these two city lakes(Xuanwu,Mochou)have similar microbial ***,the DGGE profiles of sediment samples in Lake Taihu were significantly different from these two ***,the DGGE profiles of sediment samples in different locations in Lake Taihu were also different,suggesting that the microbial communities in Lake Taihu are more diversified than those in Lake Xuanwu and Lake *** differences in microbial diversity may be caused by the different environmental conditions,such as redox potential,pH,and the concentrations of organic *** major bands of 16S rDNA genes fragments from the DGGE profiles of sediment samples were further reamplified and *** results of sequencing analysis indicate that five sequences shared 99%-100%homology with known sequences(Bacillus and Brevibacillus,uncultured bacteria),while the other two sequences shared 93%-96%homology with known sequences(Acinetobacter,and Bacillus).The study shows that the PCR-DGGE technique combined with sequenc

关键词： Sediment microbial diversity denaturing gradient gel electrophoresis(DGGE) 16S ribosome DNA(16S rDNA) sequence