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The effect of cylindrospermopsin on the bacterioplankton community:a microcosm experiment on water from Dishui Lake,Shanghai,China

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Journal of Oceanology and Limnology 2022年第5期40卷 1829-1839页

作者： Sicheng YIN Ruohan HUANG Erik JEPPESEN lijing CHEN Liqing WANG Xin fang Wei ZHANG Engineering Research Center of Environmental DNA and Ecological Water Health Assessment Shanghai Ocean UniversityShanghai 201306China Department of Ecoscience Aarhus UniversitySilkeborg 8600Denmark Sino-Danish Centre for Education and Research(SDC) University of Chinese Academy of SciencesBeijing 100049China Limnology Laboratory and EKOSAM Department of Biological SciencesMiddle East Technical UniversityAnkara 06800Turkey Institute of Marine Sciences Middle East Technical UniversityMersin 33731Turkey School of Business Macao University of Science and TechnologyTaipaMacao 999078China

Eutrophication and climate warming have intensified the global expansion of invasive cyanobacteria such as Cylindrospermopsis spp.and Chrysosporum spp.Cylindrospermopsin(CYN)produced by species of the latter two genera may harm phytoplankton,zooplankton,and fishes.However,effects of CYN on the bacterioplankton community have not been studied.Based on high-throughput sequencing,we explored the effect of CYN on the structure and function of the bacterioplankton community by adding pure CYN to in-situ water collected from a brackish coastal shallow lake:Dishui Lake,China.We found that most bacterioplankton taxa had a certain tolerance potential to CYN,but that high concentrations of CYN(40μg/L)caused a significant decrease in microbial abundance and functional groups.Of the dominant phyla,Actinobacteria had the strongest tolerance to CYN.Network analysis indicated that CYN caused shifts in the community structure of the bacterioplankton,reducing community stability and structural complexity.High CYN concentrations also reduced the correlation between the different bacterioplankton groups,and the abundances of some bacterial taxa associated with the denitrification function and the process of carbon transfer in the microbial food web were inhibited.Our study provide s new insight into the response of the bacterioplankton communities to harmful algal toxins produced by cyanobacteria.

关键词： cylindrospermopsin bacterioplankton denitrification function carbon cycles Dishui Lake

Petrophysics characteristics of coalbed methane reservoir: a comprehensive review

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Frontiers of Earth Science 2021年第2期15卷 202-223页

作者： Qifeng JIA Dameng LIU Yidong CAI Xianglong fang lijing LI School of Energy Resources China University of GeosciencesBeijing 100083China Coal Reservoir Laboratory of National Engineering Research Center of CBM Development&Utilization China University of GeosciencesBeijing 100083China

Petrophysics of coals directly affects the development of coalbed methane(CBM).Based on the analysis of the representative academic works at home and abroad,the recent progress on petrophysics characteristics was reviewed from the aspects of the scale-span porefracture structure,permeability,reservoir heterogeneity,and its controlling factors.The results showed that the characterization of pore-fracture has gone through three stages:qualitative and semiquantitative evaluation of porefracture by various techniques,quantitatively refined characterization of pore-fracture by integrating multiple methods including nuclear magnetic resonance analysis,liquid nitrogen,and mercury intrusion,and advanced quantitative characterization methods of pore-fracture by high-precision experimental instruments(focused-ion beam-scanning electron microscopy,small-angle neutron scattering and computed tomography scanner)and testing methods(m-CT scanning and X-ray diffraction).The effects of acoustic field can promote the diffusion of CBM and generally increase the permeability of coal reservoirs by more than 10%.For the controlling factors of reservoir petrophysics,tectonic stress is the most crucial factor in determining permeability,while the heterogeneity of CBM reservoirs increases with the enhancement of the tectonic deformation and stress field.The study on lithology heterogeneity of deep and high-dip coal measures,the spatial storage-seepage characteristics with deep CBM reservoirs,and the optimizing production between coal measures should be the leading research directions.

关键词： petrophysics pore-fracture permeability heterogeneity controlling factors

Oxidation behavior of ammonium in a 3-dimensional biofilm-electrode reactor

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Journal of Environmental Sciences 2013年第12期25卷 2403-2409页

作者： Jinjing Tang Jinsong Guo fang fang Youpeng Chen lijing Lei Lin Yang Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment (Ministry of Education) Chongqing University School of Chemistry and Chemical Engineering Chongqing University

Excess nitrogenous compounds are detrimental to natural water systems and to human health. To completely realize autohy- drogenotrophic nitrogen removal, a novel 3-dimensional biofilm-electrode reactor was designed. Titanium was electroplated with ruthenium and used as the anode. Activated carbon fiber felt was used as the cathode. The reactor was separated into two chambers by a permeable membrane. The cathode chamber was filled with granular graphite and glass beads. The cathode and cathode chamber were inhabited with domesticated biofilm. In the absence of organic substances, a nitrogen removal efficiency of up to 91% was achieved at DO levels of 3.42 ± 0.37 mg/L when the applied current density was only 0.02 mA/cm^2. The oxidation of ammonium in biofilmelectrode reactors was also investigated. It was found that ammonium could be oxidized not only on the anode but also on particle electrodes in the cathode chamber of the biofilm-electrode reactor. Oxidation rates of ammonium and nitrogen removal efficiency were found to be affected by the electric current loading on the biofilm-electrode reactor. The kinetic model of ammonium at different electric currents was analyzed by a first-order reaction kinetics equation. The regression analysis implied that when the current density was less than 0.02 mA/cm^2, ammonium removal was positively correlated to the current density. However, when the current density was more than 0.02 mA/cm^2, the electric current became a limiting factor for the oxidation rate of ammonium and nitrogen removal efficiency.

关键词： autotrophic biofilm-electrode nitrogen removal ammonium oxidation behavior

Cell-penetrating riboflavin conjugate for antitumor photodynamic therapy

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Chinese Chemical Letters 2022年第9期33卷 4339-4344页

作者： Chunlei Wu Yanyan Li Zhehong Cheng Pengxin Wang Zhilong Ma Ke Liu Yulian Cheng Yimin Zhou Xian Lin Ximing Shao Yong Yang Hongchang Li Lijing Fang Guangdong Key Laboratory of Nanomedicine Institute of Biomedicine and BiotechnologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China University of Chinese Academy of Sciences Beijing 100049China Department of Biomedical Engineering Southern University of Science and Technology(SUSTech)Shenzhen 518055China

Riboflavin(RF,vitamin B2)is an essential vitamin and has been considered as a promising natural photosensitizer for photodynamic therapy(PDT).However,further exploration of RF in antitumor application was limited by its poor cellular uptake.In this study,using cell-penetrating peptides Arg8,(Cha-Arg)3 and small molecule triphenylphosphine(TPP)as delivery compounds,three RF conjugates were prepared to increase the accumulation of RF in cells,termed as Arg8-RF,(Cha-Arg)3-RF and TPP-RF,respectively.Compared with TPP-RF and Arg8-RF,(Cha-Arg)3-RF exhibited better cell internalization and stronger cytotoxicity against HeLa cells upon exposure to blue light.Further researches proved that(Cha-Arg)3-RF generated reactive oxygen species(ROS)under irradiation,which could indiscriminately destroy endogenous proteins and mitochondria,ultimately inducing cell death.This work provides a new approach to explore RF as a natural photosensitizer for antitumor photodynamic therapy.

关键词： Riboflavin Antitumor Photodynamic therapy Cell-penetrating peptides

Effect of N-doping-derived solvent adsorption on electrochemical double layer structure and performance of porous carbon

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Journal of Energy Chemistry 2023年第5期80卷 120-127,I0004页

作者： Zhe-Fan Wang Cheng Tang Qian Sun Ya-Lu Han Zhi-Jian Wang lijing Xie Shou-Chun Zhang fang-Yuan Su Cheng-Meng Chen CAS Key Laboratory of Carbon Materials Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001ShanxiChina University of Chinese Academy of Sciences Bejing 100049China Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBejing 100084Chind

N-doped porous carbon has been extensively investigated for broad electrochemical applications.The performance is significantly impacted by the electrochemical double layer(EDL),which is material dependent and hard to characterize.Limited understanding of doping-derived EDL structure hinders insight into the structure-performance relations and the rational design of high-performance materials.Thus,we analyzed the mass and chemical composition variation of EDL within electrochemical operation by electrochemical quartz crystal microbalance,in-situ X-ray photoelectron spectroscopy,and time-offlight secondary ion mass spectrometry.We found that N-doping triggers specifically adsorbed propylene carbonate solvent in the inner Helmholtz plane(IHP),which prevents ion rearrangement and enhances the migration of cations.However,this specific adsorption accelerated solvent decomposition,rendering rapid performance degradation in practical devices.This work reveals that the surface chemistry of electrodes can cause specific adsorption of solvents and change the EDL structure,which complements the classical EDL theory and provide guidance for practical applications.

关键词： Carbon materials Electrochemical double layer Electrochemical quartz crystal microbalance In-situ X-ray photoelectron spectroscopy N-doping

Characterization of NPR1 and NPR4 genes from mulberry（Morus multicaulis） and their roles in development and stress resistances

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Characterization of NPR1 and NPR4 genes from mulberry（Morus...

金刚石涂层刀具钻削高比分SiC_p/Al复合材料试验研究

中国蚕学会2018年学术年会

作者： Yuqi Xu Lijing Fang Rongli Qin Zhuangliu Shuoshuo Yuan Yingping Gai Xianling Ji College of Forestry Shandong Agricultural University State Key Laboratory of Crop Biology Shandong Agricultural University

The quality and quantity of mulberry leaves are often affected by various environmental factors. The NPR1 and its homolog genes play pivotal roles in systemic acquired resistance in plants. This study, the full-length c DNAs encoding NPR1 and NPR4 genes were isolated from Husang 32（Morus multicaulis） and designated as MuN PR1 and Mu NPR4, respectively. Sequence analysis of amino acids and protein modelling of the Mu NPR1 and Mu NPR4 proteins showed that MuN PR1 share some conserved characteristics with its homologue MuN PR4. Expression analysis demonstrated that Mu NPR1 and MuN PR4 showed different expression patterns in various tissues of mulberry. Interestingly, ectopic expression of either Mu NPR1 or Mu NPR4 in Arabidopsis produced early flowering phenotype relative to wild-type plants. Further analysis indicated that the Mu NPR1 promoted the expression of pathogenesis-related 1 a gene and enhanced transgenic Arabidopsis resistance to Pseudomonas syringae pv. tomato DC3000（Pst. DC3000）. Moreover, ectopic expression of MuN PR1 might lead to the enhanced scavenging ability and suppress the collase accumulation. On the contrary, ectopic expression of MuN PR4 confers the transgenic Arabidopsis hypersensitivity to Pst. DC3000 infection. However, ectopic expression of either Mu NPR1 or Mu NPR4 gene confers the transgenic Arabidopsis hypersensitivity to drought and salt stresses. Our data suggest that both the MuN PR1 and MuN PR4 genes play a role in regulating cross-talk between signaling pathways, and the information provided here enable in-depth functional analysis of Mu NPR1 and Mu NPR4 genes and perhaps facilitates mulberry genetic improvement in the future.

关键词： Mulberry NPR1 NPR4 tissue expression pattern abiotic stress plant disease resistance

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航空制造技术 2018年第5期61卷 66-69,83页

作者：胡芳解丽静王西彬高飞农付纳新史兴宽北京理工大学机械与车辆学院北京100081 北京无线电测量研究所北京100854

SiC_p/Al复合材料与传统的金属材料相比,具有优异的物理和热学性能。但在孔加工中,棱边加工缺陷成为影响其在高端产品中使用的主要问题之一。开展金刚石涂层刀具钻削SiC_p/Al复合材料的试验研究,对试验力学信号进行分析,对出入口棱边缺... 详细信息

SiC_p/Al复合材料与传统的金属材料相比,具有优异的物理和热学性能。但在孔加工中,棱边加工缺陷成为影响其在高端产品中使用的主要问题之一。开展金刚石涂层刀具钻削SiC_p/Al复合材料的试验研究,对试验力学信号进行分析,对出入口棱边缺陷形成机理及形貌特征做出了解释。结果表明:使用金刚石涂层刀具钻削SiC_p/Al复合材料时,每步钻削深度、进给速度和主轴转速对轴向力的作用依次减弱;出口处质量明显优于入口处,入口缺陷形成机理主要是Al基体的断裂和SiC颗粒脱落;金刚石涂层钻头适合用于加工SiC_p/Al复合材料。对于SiC_p/Al复合材料的实际加工应用有一定的参考价值。

关键词： SiCp/Al 复合材料金刚石涂层钻头轴向力棱边缺陷啄钻

Advances in Researches about Immunoregulatory Functions of Dendrobium Sw.

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Medicinal Plant 2018年第2期9卷 1-4页

作者： Donghui WANG Bei FAN fang fang Yan WANG Cencan XING lijing ZHANG Fengzhong WANG Institute of Food Science and Technology Chinese Academy of Agricultural Sciences Laboratory of Quality & Safety Risk Assessment for Agricultural Products ( Beijing) Ministry of Agriculture

Based on the researches on immunoregulatory functions of Dendrobium. at home and abroad in recent years,from immune organs,immune cells,and immune molecules,this paper summarized immunoregulatory functions of polysaccharides,glycosides,and alkaloids extracted from Dendrobium. on the body,including reducing the inflammation,inhibiting tumor,and slowing down aging,to provide theoretical references for in-depth researches and development of modern biotechnology for Dendrobium.

关键词： Dendrobium Immunoregulation Anti-inflammation Anti-aging

Synthesis,Structure Characterization and Biological Activity of Adenine Salt of 12-Phosphotungstic Acid

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Chemical Research in Chinese Universities 2004年第3期20卷 258-261页

作者： LIJuan QIYan-fei HANZheng-bo WANGEn-bo lijing WUXin-yu WANGHong-fang InstituteofPolyoxometalateChemistry FacultyofChemistryNortheastNormalUniversityChangchun130024P.R.China CollegeofPublicHealth JilinUniversityChangchun130021P.R.China

A novel adenine(Ade) salt of 12-phosphotungstic acid was synthesized and characterized by elemental analyses,IR, 1H NMR,CV and single crystal X-ray diffraction. The compound crystallized in a triclinic system with a space group P 1 and a =1.3108(3) nm, b =1.3515(3) nm,c =1.3870(3) nm, V =2.0217(7) nm 3,Z =1,R _1=0.0391,wR _2=0.0959. The structure unit of the complex was constructed from [HPW_ 12 ·O_ 40 ] -,(C_5H_6N_5) + and C_3H_7NO,which further extends to form a novel three-dimensional host-guest network via hydrogen-bonding interactions. The anti-tumor activity of the compound was examined in two human tumor cell lines in vitro .

关键词： Polyoxometalate,12-Phosphotungstic acid,Adenine,Crystal structure,Anti-tumor activity