检索结果-南通市图书馆

Blood Science 2020年第4期2卷 117-128页

作者： Jiahui Zeng Wencui Sun Jing Chang Danying Yia lijiao zhu Yonggang Zhang Xu Pan Ya Zhou Mowen Lai Guohui Bian Qiongxiu Zhou Jiaxin Liu Bo Chen FengMa Institute of Blood Transfusion Chinese Academy of Medical Sciences&Peking Union Medical College(CAMS&PUMC)Chengdu 610052China State Key Laboratory of Biotherapy Sichuan UniversityChengdu 61006China State Key Laboratory of Experimental Hematology CAMS&PUMCTianjin 300020China

The hematopoietic function of HOXC4 has not been extensively investigated.Our research indicated that induction of HOXC4 in co-culture system from D10 significantly promoted productions of most hematopoietic progenitor cells.CD34−CD43+cells could be clearly classified into CD34−CD43^(low) and CD34−CD43^(high) sub-populations at D14.The former cells had greater myelogenic potential,and their production was not significantly influenced by induction of HOXC4.By contrast,the latter cells had greater potential to differentiate into megakaryocytes and erythroid cells,and thus had properties of erythroid–megakaryocyte common progenitors,which abundance was increased by∼2-fold when HOXC4 was induced from D10.For CD34−CD43^(low),CD34+CD43+,and CD34−CD43^(high) sub-populations,CD43 level served as a natural index for the tendency to undergo hematopoiesis.Induction of HOXC4 from D10 caused more CD43+cells sustain in S-phase with up-regulation of NF-κB signaling,which could be counteracted by inhibition of NF-κB signaling.These observations suggested that promotion of hematopoiesis by HOXC4 is closely related to NF-κB signaling and a change in cell-cycle status,which containing potential of clinical applications.

关键词： CD43 Erythroid–megakaryocyte common progenitor(EMkP) Hematopoiesis HOXC4 Human embryonic stem cells(hESCs) Inducible expression

Overexpression of HOXA9 upregulates NF-κB signaling to promote human hematopoiesis and alter the hematopoietic differentiation potentials

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Cell Regeneration 2021年第1期10卷 95-106页

作者： Jiahui Zeng Danying Yi Wencui Sun Yuanlin Liu Jing Chang lijiao zhu Yonggang Zhang Xu Pan Yong Dong Ya Zhou Mowen Lai Guohui Bian Qiongxiu Zhou Jiaxin Liu Bo Chen Feng Ma Chinese Academy of Medical Sciences&Peking Union Medical College(CAMS&PUMC) Institute of Blood TransfusionNo.26Huacai RoadLongtan Industry ParkChenghua DistrictChengdu 610052China State Key Laboratory of Biotherapy Sichuan UniversityChengdu 610065China State Key Laboratory of Experimental Hematology CAMS&PUMCTianjin 300020China

Background: The HOX genes are master regulators of embryogenesis that are also involved inhematopoiesis. HOXA9 belongs to a cluster of HOX genes that play extensively studied roles inhematopoiesis and leukemogenesis.Methods: We established HOXA9-inducible human embryonic stem cells (HOXA9/hESCs) with normalpluripotency and potential for hematopoiesis, which could be used to analyze gene function with highaccuracy. HOXA9/hESCs co-cultured with aorta–gonad–mesonephros-derived stromal cells (AGM-S3) wereinduced to overexpress HOXA9 with doxycycline (DOX) at various times after hematopoiesis started andthen subjected to flow cytometry.Results: Induction of HOXA9 from Day 4 (D4) or later notably promoted hematopoiesis and also increasedthe production of CD34+ cells and derived populations. The potential for myelogenesis was significantlyelevated while the potential for erythrogenesis was significantly reduced. At D14, a significant promotion ofS phase was observed in green fluorescent protein positive (GFP+) cells overexpressing HOXA9. NF-κBsignaling was also up-regulated at D14 following induction of HOXA9 on D4. All of these effects could becounteracted by addition of an NF-κB inhibitor or siRNA against NFKB1 along with DOX.Conclusions: Overexpression of HOXA9 starting at D4 or later during hematopoiesis significantly promotedhematopoiesis and the production of myeloid progenitors while reduced the production of erythroidprogenitors, indicating that HOXA9 plays a key role in hematopoiesis and differentiation of hematopoieticlineages.

关键词： Hematopoiesis Differentiation of hematopoietic lineages HOXA9 Mesoderm Human embryonic stem cells(hESCs) Tet-on Inducible expression system

Green Multicast Grooming Based on Cross-Shared Protection in Optical WDM Networks

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

China Communications 2014年第5期11卷 105-113页

作者： YU Cunqian LIU Yejun JIANG Peipei zhu lijiao GOU Lei HAO Liyuan College of Information Science and Engineering Northeastern University Shenyang 110819 P. R. China

Currently,multicast survivability in optical network obtains more attention.This paper focuses on the field of multicast grooming shared protection in green optical network and designs a novel auxiliary graph and a shared matrix to address the survivable multicast resource consumption.This paper also proposes a new heuristic called Green Multicast Grooming algorithm based on spanning path Cross-Shared protection(GMGCSP),in which network reliability,energy efficiency and resource utilization are jointly considered.Simulation results testify that,compared to other algorithms,the proposed GMG-CSP not only has lower blocking probability and higher bandwidth utilization ratio,but also saves more energy.

关键词： WDM光网络共享保护组播美容资源利用率 CSP封装带宽利用率资源消耗

Decoding m^(6)A mRNA methylation by reader proteins in liver diseases

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2024年第2期11卷 711-726页

作者： lijiao Sun Xin Chen Sai zhu Jianan Wang Shaoxi Diao Jinyu Liu Jinjin Xu Xiaofeng Li Yingyin Sun Cheng Huang Xiaoming Meng Xiongwen Lv Jun Li Inflammation and Immune Mediated Diseases Laboratory of Anhui Province Anhui Institute of Innovative DrugsSchool of PharmacyAnhui Medical UniversityHefeiAnhui 230032China The Key Laboratory of Anti-inflammatory and Immune Medicines Anhui Medical UniversityMinistry of EducationHefeiAnhui 230032China Institute for Liver Diseases of Anhui Medical University ILD-AMUAnhui Medical UniversityHefeiAnhui 230032China Department of Nephropathy The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China Department of Oncology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230032China

N6-methyladenosine(m^(6)A)is a dynamic and reversible epigenetic regulation.As the most prevalent internal post-transcriptional modification in eukaryotic RNA,it participates in the regulation of gene expression through various mechanisms,such as mRNA splicing,nuclear export,localization,translation efficiency,mRNA stability,and structural transformation.The involvement of m^(6)A in the regulation of gene expression depends on the specific recognition of m^(6)A-modified RNA by reader proteins.In the pathogenesis and treatment of liver disease,studies have found that the expression levels of key genes that promote or inhibit the development of liver disease are regulated by m^(6)A modification,in which abnormal expression of reader proteins determines the fate of these gene transcripts.In this review,we introduce m^(6)A readers,summarize the recognition and regulatory mechanisms of m^(6)A readers on mRNA,and focus on the biological functions and mechanisms of m^(6)A readers in liver cancer,viral hepatitis,non-alcoholic fatty liver disease(NAFLD),hepatic fibrosis(HF),acute liver injury(ALI),and other liver diseases.This information is expected to be of high value to researchers deciphering the links between m^(6)A readers and human liver diseases.

关键词： IGF2BPs Liver diseases m^(6)A modification m^(6)A reader mRNA metabolism YTH domain protein

局部涂抹复方利多卡因乳膏在预防患儿静脉穿刺疼痛中的价值研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中外医学研究 2022年第2期20卷 91-94页

作者：祝丽娇厦门大学附属妇女儿童医院厦门市妇幼保健院福建厦门361000

目的:研究局部涂抹复方利多卡因乳膏在预防患儿静脉穿刺疼痛中的价值。方法:选取2019年1月-2020年12月厦门大学附属妇女儿童医院小儿外科收治的86例需行静脉留置针穿刺患儿,随机分为常规组与研究组,每组43例。常规组患儿皮肤常规消毒后... 详细信息

目的:研究局部涂抹复方利多卡因乳膏在预防患儿静脉穿刺疼痛中的价值。方法:选取2019年1月-2020年12月厦门大学附属妇女儿童医院小儿外科收治的86例需行静脉留置针穿刺患儿,随机分为常规组与研究组,每组43例。常规组患儿皮肤常规消毒后随即穿刺,研究组患儿皮肤局部涂抹复方利多卡因乳膏后再行穿刺,对比两组患儿穿刺前后的心率、平均动脉压、穿刺后的疼痛程度、不良反应发生率及家属满意程度。结果:常规组穿刺后30 min儿童疼痛评估量表(FLACC)评分与穿刺后5 min比较差异无统计学意义(P>0.05);研究组穿刺后30 min FLACC评分低于穿刺后5 min,且研究组穿刺后5、30 min FLACC评分均低于常规组(P0.05);研究组穿刺后5 min心率、平均动脉压均小于常规组(P<0.05)。研究组不良反应发生率为4.65%,低于常规组的18.60%(P<0.05)。研究组家属满意度为97.67%,高于常规组的79.07%(P<0.05)。结论:在静脉留置针患儿静脉穿刺前局部涂抹复方利多卡因乳膏,有利于预防或减轻静脉穿刺疼痛,降低患儿应激反应,减少其他不良反应的发生,患儿家属满意度高。

关键词：复方利多卡因乳膏患儿静脉穿刺静脉留置针

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

麻醉复苏室的安全隐患与防范管理措施

中国卫生产业 2022年第15期19卷 98-101页

作者：祝丽娇林冬红林鹏厦门大学附属妇女儿童医院(厦门市妇幼保健院)麻醉科福建厦门361000

目的分析麻醉复苏室安全管理中采用针对性管理措施对安全隐患的影响。方法选取2020年7月—2021年6月在厦门大学附属妇女儿童医院工作的30名麻醉复苏室医务人员为研究对象,2020年7—12月麻醉复苏室采取常规护理管理模式,纳入对照组;2021... 详细信息

目的分析麻醉复苏室安全管理中采用针对性管理措施对安全隐患的影响。方法选取2020年7月—2021年6月在厦门大学附属妇女儿童医院工作的30名麻醉复苏室医务人员为研究对象,2020年7—12月麻醉复苏室采取常规护理管理模式,纳入对照组;2021年1—6月麻醉复苏室总结上一阶段工作开展中存在的安全隐患后采取针对性防范管理措施,纳入研究组,另选择120例本院麻醉复苏室接收的患者为调查对象。分析两组管理效果。结果研究组文书记录(23.24±0.55)分、护理态度(23.17±0.87)分、护理技能(23.28±0.43)分、安全管理(23.44±0.87)分、总分(95.49±1.27)分均高于对照组,差异有统计学意义(t=5.260、4.563、5.025、4.938、4.517,P<0.05);其增强专业技能(96.27±1.87)分、合理使用药物(96.42±1.55)分、提升沟通水平(96.72±1.43)分均高于对照组,差异有统计学意义(t=4.157、4.524、5.285,P<0.05);其患者投诉率1.67%低于对照组,差异有统计学意义(χ2=4.821,P<0.05);其护理总满意度96.67%高于对照组,差异有统计学意义(χ2=6.987,P<0.05)。结论目前麻醉复苏室仍有一定的安全隐患,根据实际情况展开针对性的管理措施,能显著提升管理效果,提高护理满意度。

关键词：安全隐患麻醉复苏室防范管理措施满意度

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

分析轮流组长制在麻醉护士管理中的应用效果

中国卫生产业 2022年第16期19卷 65-68页

作者：祝丽娇林冬红林鹏厦门大学附属妇女儿童医院(厦门市妇幼保健院)麻醉科福建厦门361000

目的分析在麻醉护士管理中应用轮流组长制的实施效果。方法选取2020年1月—2021年12月入厦门大学附属妇女儿童医院麻醉护士8名为研究对象,在2020年1—12月,采取常规管理制度,即由1名工作经验丰富的麻醉护士担任组长,有关数据分入对照组;... 详细信息

目的分析在麻醉护士管理中应用轮流组长制的实施效果。方法选取2020年1月—2021年12月入厦门大学附属妇女儿童医院麻醉护士8名为研究对象,在2020年1—12月,采取常规管理制度,即由1名工作经验丰富的麻醉护士担任组长,有关数据分入对照组;在2021年1—12月采取轮流组长管理制度,即每6个月由1名麻醉护士轮流担任组长,有关数据分入改进组。对二者数据统计结果实施对照分析。结果改进组基础服务(23.27±0.54)分、科室环境(23.18±0.86)分、职业能力(23.25±0.42)分、专科服务(23.48±0.81)分、总分(96.44±1.26)分均高于对照组,差异有统计学意义(t=2.653、3.411、4.348、3.736、2.788,P<0.05);其投诉率0.50%、不良事件发生率1.00%低于对照组,且满意度98.50%高于对照组,差异有统计学意义(χ2=4.092、4.580、10.315,P<0.05);其大科考核理论知识(98.52±1.05)分、实践技能(98.35±1.14)分及护理部考核理论知识(98.47±1.08)分、实践技能(98.26±1.33)分均高于对照组,差异有统计学意义(t=3.309、3.037、4.847、3.905,P<0.05)。结论通过实施轮流组长制,能够在麻醉护士管理中发挥积极价值,对提高管理质量、优化管理效果具有重要作用。

关键词：麻醉护士轮流组长制管理质量

不同浓度罗哌卡因腰硬联合麻醉对妊娠期糖尿病产妇剖宫产术后VAS评分及MAP指标的影响

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

糖尿病新世界 2023年第7期26卷 14-17,22页

作者：吴灵肖夏静祝丽娇厦门大学附属妇女儿童医院(厦门市妇幼保健院)麻醉科福建厦门361000

目的分析妊娠期糖尿病(gestational diabetes mellitus,GDM)产妇采取不同浓度的罗哌卡因腰硬联合麻醉实施剖宫产术对其MAP指标和VAS评分的影响。方法选取2021年2月—2022年2月厦门大学附属妇女儿童医院(厦门市妇幼保健院)收治的采取罗... 详细信息

目的分析妊娠期糖尿病(gestational diabetes mellitus,GDM)产妇采取不同浓度的罗哌卡因腰硬联合麻醉实施剖宫产术对其MAP指标和VAS评分的影响。方法选取2021年2月—2022年2月厦门大学附属妇女儿童医院(厦门市妇幼保健院)收治的采取罗哌卡因麻醉的剖宫产手术的GDM产妇90例为研究对象,随机分为A组、B组、C组,各30例,3组分别采用0.25%、0.375%、0.5%的罗哌卡因,对比3组麻醉相关指标、生命体征、术后VAS评分、微循环指标及不良反应情况。结果与A组和B组相比,C组感觉阻滞平面、Bromage评分更高,感觉阻滞时间更长,剖宫产术后2、4 h的静态VAS评分、动态VAS评分更低,术后24、48 h甲襞微循环指标更高,差异有统计学意义(P<0.05)。与B组和C组相比,A组麻醉15 min时,MAP更高、HR更低、不良反应更少,差异有统计学意义(P<0.05)。结论对于GDM产妇剖宫产来讲,浓度不同的罗哌卡因麻醉效果均良好,其中浓度0.5%有更好的镇痛效果,且对微循环影响更小;0.25%对血流动力学影响更小,不良反应更少.

关键词：剖宫产 MAP指标罗哌卡因 VAS评分妊娠期糖尿病不良反应腰硬联合麻醉