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Effect of electroacupuncture in Weijing points on gastrointestinal interdigestive migrating motor complex and brain gut peptides release in dogs

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Chinese Science Bulletin 2001年第10期46卷 843-850页

作者： ZHOU Lu wang lijian YUN Tianyou wang Ling Department of Physiology Institute of Basic Medical ScienceChinese Academy of Medical Sciences and School of Basic MedicinePeking Union of Medical CollegeBeijing 100005China

Interdigestive gastrointestinal migrating motor complex (MMC) activities were recorded by strain gauge implanted on the serosa in 7 conscious dogs. We studied the effects of electroacupuncture (EAP) Weijing points Zusanli (S36), Tianshu (S25), Liangmen (S21) on MMC and release of motilin and gastrin, and compared them with that of EAP Pangguangjing points. The results indicated that EAP Weijing points could not only strengthen MMC contractions in antrum, duodenum and proximal jejunum, but also increase plasma concentration of motilin and gastrin. Anti-motilin serum, proglumide, atropine, or hexamethonium could markedly block the effect of EAP on reinforcing MMC contraction and release of motilin and gastrin. We could get the conclusions that such enhancing effect of EAP Weijing points on MMC and brain-gut peptides release is mediated by motilin and gastrin, on which both cholinergic nerve and sympathetic nerve play important roles.

关键词： interdigestive migrating motor complex (MMC) elec-troacupuncture motilin gastrin.

Arabidopsis phytochromes A and B synergistically repress SPA1 under blue light

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Journal of Integrative Plant Biology 2023年第4期65卷 888-894页

作者： Xiaolin Jia Meifang Song Shaoci wang Tong Liu lijian wang Lin Guo Liang Su Yong Shi Xu Zheng Jianping Yang State Key Laboratory of Wheat and Maize Crop Science and Center for Crop Genome EngineeringLongzi Lake CampusCollege of AgronomyHenan Agricultural UniversityZhengzhou 450046China Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Henan Police College Zhengzhou 450046China Beijing Genseed Technology Co. Ltd.Beijing 100080China

In Arabidopsis,although studies have demonstrated that phytochrome A(phyA)and phyB are involved in blue light signaling,how blue light-activated phytochromes modulate the activity of the CONSTITUTIVELY PHOTOMORPHOGENIC1(COP1)-SUPPRESSOR OF PHYA-105(SPA1)E3 complex remains largely ***,we show that phyA responds to early and weak blue light,whereas phyB responds to sustainable and strong blue *** of both phyA and phyB by blue light inhibits SPA1 ***,blue light irradiation promoted the nuclear import of both phytochromes to stimulate their binding to SPA1,abolishing SPA1’s interaction with LONG HYPOCOTYL 5(HY5)to release HY5,which promoted seedling photomorphogenesis.

关键词： Arabidopsis blue light phytochrome SPA1

Importers Drive Leaf-to-Leaf Jasmonic Acid Transmission in Wound-Induced Systemic Immunity

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Molecular Plant 2020年第10期13卷 1485-1498页

作者： Mengya Li Feifei wang Shuangzhang Li Guanghui Yu lijian wang Qingqing Li Xiangyu Zhu Zhen Li Lixing Yuan Pei Liu Department of Ecology College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193P.R.China State Key Laboratory of Plant Physiology and Biochemistry College of Biological SciencesChina Agricultural UniversityBeijing 100193P.R.China Department of Plant Nutrition College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193P.R.China

The transmission of mobile wound signals along the phloem pathway is essential to the activation of wound-induced systemic response/resistance,which requires an upsurge of jasmonic acid(JA)in the distal undamaged *** these mobile signals,the electrical signal mediated by the glutamate-dependent activation of several clade three GLUTAMATE RECEPTOR-LIKE(GLR3)proteins is involved in the stimulation of JA production in distal ***,whether JA acts as a mobile wound signal and,if so,how it is transmitted and interacts with the electrical signal remain ***,we show that JA was translocated from the local to distal leaves in Arabidopsis,and this process was predominantly regulated by two phloem-expressed and plasma membrane-localized jasmonate transporters,AtJAT3 and *** addition to the cooperation between AtJAT3/4 and GLR3.3 in the regulation of long-distance JA translocation,our findings indicate that importer-mediated cell-cell JA transport is important for driving the loading and translocation of JA in the phloem pathway in a self-propagating manner.

关键词： wound-induced systemic resistance jasmonate importers mobile signals phloem loading long-distance transmission self-propagation mode

Effects of free-air CO_2 enrichment on adventitious root development of rice under low and normal soil nitrogen levels

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The Crop Journal 2014年第4期2卷 207-212页

作者： Chengming Sun lijian wang Tao Liu Doudou Guo Yingying Chen Wei Wu Yulong wang Jianguo Zhu Key Laboratory of Crop Genetics and Physiology of Jiangsu Province College of Agriculture Yangzhou University Institute of Soil Science Chinese Academy of Sciences

Free air CO2 enrichment(FACE) and nitrogen(N) have marked effects on rice root growth,and numerical simulation can explain these effects. To further define the effects of FACE on root growth of rice, an experiment was performed, using the hybrid indica cultivar Xianyou63. The effects of increasing atmospheric CO2 concentration [CO2], 200 μmol mol-1higher than ambient, on the growth of rice adventitious roots were evaluated, with two levels of N: low(LN, 125 kg ha-1) and normal(NN, 250 kg ha-1). The results showed a significant increase in both adventitious root number(ARN) and adventitious root length(ARL) under FACE treatment. The application of nitrogen also increased ARN and ARL, but these increases were smaller than that under FACE treatment. On the basis of the FACE experiment, numerical models for rice adventitious root number and length were constructed with time as the driving factor. The models illustrated the dynamic development of rice adventitious root number and length after transplanting, regulated either by atmospheric [CO2] or by N *** simulation result was supported by statistical tests comparing experimental data from different years, and the model yields realistic predictions of root growth. These results suggest that the models have strong predictive potential under conditions of atmospheric [CO2] rises in the future.

关键词： Rice Free air CO2enrichment(FACE) Root number Root length Model

Transporter-Mediated Nuclear Entry of Jasmonoyl-lsoleucine Is Essential for Jasmonate Signaling

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Molecular Plant 2017年第5期10卷 695-708页

作者： Qingqing Li Jian Zheng Shuaizhang Li Guanrong Huang Stephen J. Skilling lijian wang Ling Li Mengya Li Lixing Yuan Pei Liu Department of Ecology Department of Plant Nutrition College of Resources and Environmental Sciences China Agricultural University Beijing 100193 P. R. China

To control gene expression by directly responding to hormone concentrations, both animal and plant cells have exploited comparable mechanisms to sense small-molecule hormones in nucleus. Whether nuclear entry of these hormones is actively transported or passively diffused, as conventionally postulated, through the nuclear pore complex, remains enigmatic. Here, we identified and characterized a jasmonate transporter in Arabidopsis thaliana, AtJAT1/AtABCG16, which exhibits an unexpected dual localization at the nuclear envelope and plasma membrane. We show that AtJAT1/AtABCG16 controls the cytoplasmic and nuclear partition of jasmonate phytohormones by mediating both cellular efflux of jasmonic acid （JA） and nuclear influx of jasmonoyl-isoleucine （JA-Ile）, and is essential for maintaining a critical nuclear JA-Ile concentration to activate JA signaling. These results illustrate that transporter-mediated nuclear entry of small hormone molecules is a new mechanism to regulate nuclear hormone signaling. Our findings provide an avenue to develop pharmaceutical agents targeting the nuclear entry of small molecules.

关键词： jasmonate transporter jasmonic acid jasmonoyl-isoleucine nuclear import AtJAT1/AtABCG16

Reduced dietary nitrogen with a high Lys:CP ratio restricted dietary N excretion without negatively affecting weaned piglets

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Animal Nutrition 2019年第2期5卷 115-123页

作者： Hongnan Liu Li Wu Hui Han Yuying Li lijian wang Jie Yin Wenjun Fan Miaomiao Bai Jiming Yao Xingguo Huang Tiejun Li Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process Key Laboratory of Agro-Ecological Processes in Subtropical RegionNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry ProductionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China Hunan Co-Innovation Center of Animal Production Safety Changsha410128China University of Chinese Academy of Sciences Beijing100039China Department of Animal Science Hunan Agriculture UniversityChangsha410128China Guangdong wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine Guangdong Wangda Group Co.Ltd.Guangzhou510663China

We hypothesized that balancing the content of exogenous amino acids, especially lysine, to reduce protein content in swine diets could reduce nitrogen(N) pollution associated with animal husbandry. Two experiments(45 d each experiment) were performed on weaned piglets(Duroc x Landrace x Yorkshire,28 d of age) to test this and to determine the optimal lysine to crude protein(Lys:CP) ratio in diet. In Exp. 1,12 piglets(6 replicates [n = 6]) were fed diets containing different levels of CP(17% and 20%) but the same level of Lys, Increased CP content resulted in significant increases(P < 0.05) of average daily gain(ADG), average daily feed intake(ADFI), and body weight(BW);but did not affect the feed to gain ratio. In Exp. 2,24 piglets(8 replicates [n = 8]) were fed 1 of 3 diets as follows: 1) 20% CP with a regular Lys:CP ratio(6.23%, control);2) 17% CP with a reduced Lys:CP ratio(6.14%, LL);or 3) 17% CP with a standard Lys:CP ratio(7.32%, SL). The ADG, final BW, serum concentrations of growth hormone and insulin-like growth factor-1, villus height in the jejunum, and villus height to crypt depth ratio were the lowest in piglets fed LL diet, whereas blood urea N concentration was the lowest and the value of lipase activity was the highest in the piglets fed SL diet. The SL diet did not affect growth performance,intestinal morphology, or serum hormone concentrations, indicating that reduced dietary N with a high Lys:CP ratio can efficiently reduce dietary N excretion without negatively affecting weaned piglets.

关键词： Protein Lysine Feeding Digestion Nutrition Genetic mapping

Maize cryptochromes 1a1 and 1a2 promote seedling photomorphogenesis and shade resistance in Zea mays and Arabidopsis

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The Crop Journal 2023年第4期11卷 1192-1203页

作者： Xiaocong Fan Shizhan Chen Wenjing Wu Meifang Song Guanghua Sun Shuaitao Yao Weimin Zhan Lei Yan Hongdan Li Yanpei Zhang lijian wang Kang Zhang Liangliang Jiang Jianping Yang Qinghua Yang College of Agronomy State Key Laboratory of Wheat and Maize Crop Scienceand Center for Crop Genome EngineeringLongzi Lake CampusHenan Agricultural UniversityZhengzhou 450046HenanChina Institute of Radiation Technology Beijing Academy of Science and TechnologyBeijing 100875China Institute of Crops Jiangxi Academy of Agricultural SciencesNanchang 330200JiangxiChina Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Genovo Biotechnology Co. Ltd.Tianjin 301700China Henan Institute of Science and Technology Xinxiang 453003HenanChina

Maize growth and development are regulated by light quality,intensity and *** are blue/ultraviolet-A light receptors involved in stem elongation,shade avoidance,and photoperiodic *** investigate the function of cryptochrome 1(CRY1) in maize,where it is encoded by Zm CRY1,we obtained two Zm CRY1a genes(Zm CRY1a1 and Zm CRY1a2),both of which share the highest similarity with other gramineous plants,in particular rice CRY1a by phylogenetic *** Arabidopsis,overexpression of Zm CRY1a genes promoted seedling de-etiolation under blue and white light,resulting in dwarfing of mature *** seedlings of the maize inbred line Zong 31(Zm CRY1aOE),overexpression of Zm CRY1a genes caused a reduction in the mesocotyl and first leaf sheath lengths due to down-regulation of genes influencing cell *** mature transgenic maize plants,plant height,ear height,and internode length decreased in response to overexpression of Zm CRY1a *** of Zm CRY1a were insensitive to low blue light(LBL)-induced shade avoidance syndrome(SAS) in Arabidopsis and *** prompted us to investigate the regulatory role of the gibberellin and auxin metabolic pathways in the response of Zm CRY1a genes to LBL *** confirmed a link between Zm CRY1a expression and hormonal influence on the growth and development of maize under LBL-induced *** results reveal that Zm CRY1a has a relatively conservative function in regulating maize photomorphogenesis and may guide new strategies for breeding high density-tolerant maize cultivars.

关键词： Zea mays L.Cryptochrome Photomorphogenesis Shade avoidance syndrome Hormone

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高位水箱雨水收集系统工程应用要点

给水排水 2022年第S01期48卷 355-357页

作者：王利建中国联合工程有限公司杭州310052

目前给排水设计中的雨水收集系统越来越被广泛的应用,但传统的雨水收集系统虽然比较成熟,但也存在一些设计缺陷。比如,水质容易受污染,收集、回收管网较多等。通过实际案例详细介绍了高位水箱雨水收集系统的原理和优点,并提出了实际工... 详细信息

目前给排水设计中的雨水收集系统越来越被广泛的应用,但传统的雨水收集系统虽然比较成熟,但也存在一些设计缺陷。比如,水质容易受污染,收集、回收管网较多等。通过实际案例详细介绍了高位水箱雨水收集系统的原理和优点,并提出了实际工程中实施该系统应注意的事项。

关键词：雨水收集高位水箱初期雨水溢流

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走向“他者”的比较文学——评苏源熙《“制造”野蛮人:中国文学和多语亚洲》

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中国比较文学 2024年第3期 280-284页

作者：王礼健上海师范大学人文学院安庆师范大学

自上世纪60年代以来,关于比较文学学科建制的“危机说”“死亡说”不绝于耳。在此背景下,斯皮瓦克(Gayatri Spivak)在《一门学科之死》(Death of a Discipline, 2003)中呼吁一种“走向他者”的、新的比较文学,即与区域研究携手,关注世... 详细信息

自上世纪60年代以来,关于比较文学学科建制的“危机说”“死亡说”不绝于耳。在此背景下,斯皮瓦克(Gayatri Spivak)在《一门学科之死》(Death of a Discipline, 2003)中呼吁一种“走向他者”的、新的比较文学,即与区域研究携手,关注世界无数本土语言和习语、连接人文学科和社会学科,同时属于南半球的、新的比较文学。

关键词：本土语言社会学科斯皮瓦克比较文学人文学科习语他者