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Cellulose Nanofibrous Membranes Modified with Phenyl Glycidyl Ether for Efficient Adsorption of Bovine Serum Albumin

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Advanced Fiber Materials 2019年第3期1卷 188-196页

作者： lihuan wang Qiuxia Fu Jianyong Yu Lifang Liu Bin Ding State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of TextilesDonghua UniversityShanghai 201620China Innovation Center for Textile Science and Technology Donghua UniversityShanghai 200051China

Hydrophobic interaction chromatography(HIC)as an indispensable method for protein purification has attracted considerable attentions of researchers as well as biopharmaceutical ***,the low binding capacity and slow adsorption rate of the currently available HIC media lead to a little supply and high price of the highly purified ***,nanofibrous membranes with hydrophobic binding sites were developed for HIC by directly coupling phenyl glycidyl ether on the hydrolyzed cellulose acetate nanofiber membrane(cellulose-phenyl NFM).Scanning electron microscope(SEM),water contact angle(WCA),Fourier transform infrared(FTIR),thermogravimetric analysis(TGA),Brunauer-Emmett-Teller(BET)surface area analysis and capillary flow porometer(CFP)were applied to evaluate the physically and chemically structural *** obtained cellulose-phenyl NFMs showed a proper hydrophilcity(WCA=37°),a relatively high BET surface area(3.6 times the surface area of commercial fibrous membranes),and tortuous-channel structure with through-hole size in the range of 0.25-1.2μm,which led to a little non-specificity adsorption,high bovine serum albumin adsorption capacity of 118 mg g^(−1),fast adsorption process within 12 h,good long-term stability and ***,compared with traditional modification methods which always include activation and graft two steps,direct coupling method is more efficient for HIC media ***,cellulose-phenyl NFMs with outstanding protein adsorption performance could be a kind of promising candidate for HIC.

关键词： Cellulose nanofibers Phenyl glycidyl ether Protein adsorption Hydrophobic interaction chromatography

Al^(3+)-induced vesicle formation

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Chinese Science Bulletin 2007年第19期52卷 2600-2604页

作者： DONG RenHao wang lihuan YUAN ZaiWu SONG AiXin HAO JingCheng Key Laboratory of Colloid and Interface Chemistry of Ministry of Education Shandong University Jinan 250100 China

Aluminium laurate [Al(OOCC11H23)3] was synthesized as a surfactant, which can dissolve in micellar solution of a zwitterionic surfactant, tetradecycldimethylamine oxide (C14DMAO). The phase behavior of the mixtures of Al(OOCC11H23)3 and C14DMAO in water was studied and birefringent Lα-phase was observed. The birefringent Lα-phase consists of vesicles that were demonstrated by Polarizer and Transmission Electron Microscope (TEM) micrographs. Al3+-coordinated vesicles could be used as templating-precursor, providing a vesicle-route for preparation of inorganic nanoscale particles.

关键词：感应气泡铝离子二甲胺月桂酸酯

Electrospun Nanofibrous Materials for Wound Healing

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Advanced Fiber Materials 2020年第4期2卷 212-227页

作者： Yuping Dong Yuqi Zheng Keyan Zhang Yueming Yao lihuan wang Xiaoran Li Jianyong Yu Bin Ding State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of TextilesDonghua UniversityShanghai 201620China College of Materials Science and Engineering Donghua UniversityShanghai 201620China Innovation Center for Textile Science and Technology Donghua UniversityShanghai 200051China

Wound dressing materials which are capable of meeting the demands of accelerating wound closure and promoting wound healing process have being highly *** nanofibrous materials show great application potentials for wound healing owing to relatively large surface area,better mimicry of native extracellular matrix,adjustable waterproofness and breathability,and programmable drug delivery *** this review article,we begin with a discussion of wound healing process and current commercial wound dressing ***,we emphasize on electrospun nanofibrous materials for wound dressing,covering the efforts for controlling fiber alignment and morphology,constructing 3D scaffolds,developing waterproof-breathable membrane,governing drug delivery performance,and regulating stem cell ***,we finish with challenges and future prospects of electrospun nanofibrous materials for wound dressings.

关键词： Electrospun Wound dressings Waterproofness Breathability Drug delivery Stem cell therapy

First report of Alternaria alternata causing flower blight on Camellia sinensis in Hefei,China

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Beverage Plant Research 2022年第1期2卷 129-132页

作者： Xiang Xie Qingjie Yu Xinye Li Yongsheng Liu lihuan wang School of Horticulture State Key Laboratory of Tea Plant Biology and UtilizationAnhui Agricultural UniversityHefei 230036China Ministry of Education Key Laboratory for Bio-resource and Eco-environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China

Flowers are an essential organ for sexual reproduction of higher *** lesions along with flower blight on tea(Camellia sinensis)plants were observed in the experimental tea plantation located in Hefei(China).The pathogens isolated from diseased flowers matched the morphological peculiarity of Alternaria *** species characteristics of *** were further confirmed by both pathogenicity tests and multi-gene phylogenetic analyses by using internal transcribed spacer(ITS),glyceraldehyde-3-phosphate dehydrogenase(GAPDH)and betatubulin(TUB).The combined phylogeny analysis using sequences derived from the ITS,GAPDH and TUB showed that the isolated pathogens belong to the genus *** tests conducted on healthy tea flowers and leaves manifested typical symptoms of flower blight while weaker symptoms of leaf spot,demonstrating the *** isolates were the causal agents of flower blight disease on tea *** fungus is first reported as a pathogen causing flower blight on *** in this study.

关键词： phylogeny flowers blight

Analysis of the Protein Electrophoresis Patterns of Curvularia Lunata in Maize and Immunological Detection of the Pathogenic Culture

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High Technology Letters 2002年第3期8卷 11-15页

作者：宁红 Gao Rong QIN Zhen wang lihuan LIU Shigui National Lab. of Biocontrol Engineering of Grassland Pests of Sichuan University Chengdu 610064 P.R.China Plant Quarantine Station of Sichuan Agricultural Department No.4 Wuhouci Street Chengdu 610041 P.R.China National Lab. of Biocontrol Engineering of Grassland Pests of Sichuan University Chengdu 610064 P.R.China Plant Quarantine Station of Sichuan Agricultural Department No.4 Wuhouci Street Chengdu 610041 P.R.China

Twelve isolates of Curvularia lunata and six related species from maize or other host plants are examined for the analysis of polyacrylamide gel electrophoresis(SDS PAGE) patterns of their soluble proteins. Differences exist significantly among different species and the same species. There is a special protein band for Curvularia at relative mobility value 0.177 (Rf 0.177) and a special protein band for C. lunata at Rf 0.225. Two kinds of primary antibodies to C. lunata are used to indirect ELISA and Avidin Biotin peroxidase Complex (ABC) immunoassay to evaluate their detecting sensitivity and specificity. The sensitivity of ABC immuno assay is higher than indirect ELISA. APAbs to C. lunata is special to C. lunata.

关键词： maize Curvularia lunata SDS PAGE immunological detection

Kiwifruit Genome Database(KGD):a comprehensive resource for kiwifruit genomics

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Horticulture Research 2020年第1期7卷 1076-1083页

作者： Junyang Yue Jiacheng Liu Wei Tang Ya Qing Wu Xiaofeng Tang Wei Li Ying Yang lihuan wang Shengxiong Huang Congbing Fang Kun Zhao Zhangjun Fei Yongsheng Liu Yi Zheng School of Horticulture Anhui Agricultural UniversityHefei 230036China School of Food and Biological Engineering Hefei University of TechnologyHefei 230009China Boyce Thompson Institute Cornell UniversityIthacaNY 14853USA USDA-Agricultural Research Service Robert W.Holley Center for Agriculture and HealthIthacaNY 14853USA Ministry of Education Key Laboratory for Bio-resource and Eco-environment College of Life ScienceState Key Laboratory of Hydraulics and Mountain River EngineeringSichuan UniversityChengdu 610064China Beijing Advanced Innovation Center for Tree Breeding by Molecular Design Beijing University of AgricultureBeijing 102206China Plant Science and Technology College Beijing University of AgricultureBeijing 102206China

Kiwifruit(Actinidia spp.)plants produce economically important fruits containing abundant,balanced phytonutrients with extraordinarily high vitamin C *** the release of the first kiwifruit reference genome sequence in 2013,large volumes of genome and transcriptome data have been rapidly accumulated for a handful of kiwifruit *** efficiently store,analyze,integrate,and disseminate these large-scale datasets to the research community,we constructed the Kiwifruit Genome Database(KGD;http://***/).The database currently contains all publicly available genome and gene sequences,gene annotations,biochemical pathways,transcriptome profiles derived from public RNA-Seq datasets,and comparative genomic analysis results such as syntenic blocks and homologous gene pairs between different kiwifruit genome assemblies.A set of user-friendly query interfaces,analysis tools and visualization modules have been implemented in KGD to facilitate translational and applied research in kiwifruit,which include JBrowse,a popular genome browser,and the NCBI BLAST sequence search *** notable tools developed within KGD include a genome synteny viewer and tools for differential gene expression analysis as well as gene ontology(GO)term and pathway enrichment analysis.

关键词： comprehensive fruit

DNA与非离子表面活性剂复配体系水溶液聚集行为

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科学通报 2017年第6期62卷 606-615页

作者：王丽焕张琪郝京诚山东大学胶体与界面化学教育部重点实验室济南250100

自组装构建DNA的纳米复合物以提高其基因治疗效果,是目前化学、生物和医药学交叉领域的一个研究热点.本文研究了非离子表面活性剂十二烷基聚四氧乙烯醚(C_(12)EO_4)与DNA之间弱相互作用构筑的复配体系溶液聚集体,探讨了复配溶液聚集体... 详细信息

自组装构建DNA的纳米复合物以提高其基因治疗效果,是目前化学、生物和医药学交叉领域的一个研究热点.本文研究了非离子表面活性剂十二烷基聚四氧乙烯醚(C_(12)EO_4)与DNA之间弱相互作用构筑的复配体系溶液聚集体,探讨了复配溶液聚集体的组装结构、相结构转变与性能.通过温度改变控制DNA与C_(12)EO_4相互作用过程,确定了复配体系溶液聚集体结构的可控转变,建立了一种可控的组装方法.研究结果对DNA与表面活性剂复配溶液聚集体的性质与结构的组装规律具有重要意义.

关键词： DNA 非离子表面活性剂弱相互作用层状相 Cryo-TEM

CLM5在中国西南地区植被生长模拟的适用性分析

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高原气象 2024年第6期43卷 1586-1599页

作者：王黎欢吕雅琼王梓奕中国科学院、水利部成都山地灾害与环境研究所四川成都610299 中国科学院大学北京100049 成都信息工程大学大气科学学院/高原大气与环境四川省重点实验室四川成都610225

在全球变暖的背景下,西南地区整体上气温升高降水减少,作为中国重要的碳汇地区,西南地区的植被动态监测与模拟对深入了解其碳循环机制和促进经济可持续发展具有重要意义。本研究利用陆面过程模式(Community Land Model version5, CLM5)... 详细信息

在全球变暖的背景下,西南地区整体上气温升高降水减少,作为中国重要的碳汇地区,西南地区的植被动态监测与模拟对深入了解其碳循环机制和促进经济可持续发展具有重要意义。本研究利用陆面过程模式(Community Land Model version5, CLM5),模拟和分析西南地区2000-2016年叶面积指数LAI(Leaf Area Index, LAI)和总初级生产力GPP(Gross Primary Productivity, GPP)的时空变化特征,并与多套遥感数据进行对比,评估CLM5在西南地区对LAI和GPP模拟的适用性。研究结果表明,CLM5能较好地模拟西南地区LAI和GPP的季节变化规律,但模拟存在对LAI生长季的高估,以及对GPP全年低估,对温带落叶阔叶灌木的LAI、高寒C3草甸的LAI、 GPP和C3草甸的GPP模拟效果较好。CLM5能够较好地刻画西南地区LAI和GPP空间分布格局,表现为由东南向西北递减,但整体上CLM5对西南地区LAI模拟偏高,特别是对贵州喀斯特地貌地区LAI的模拟偏高。与模型对LAI模拟高估相反,CLM5对西南地区GPP的模拟整体偏低,特别是云南地区。此外,CLM5对西南地区LAI和GPP的变化趋势模拟效果较差,特别是在云南大部分地区,遥感数据主要呈现上升趋势,而CLM5模拟呈现下降趋势。整体上,CLM5能模拟出西南地区LAI和GPP的季节变化规律和空间分布,但对云南和贵州部分地区的变化趋势模拟较差,仍需要针对四川盆地农田、云南森林、和贵州喀斯特地区植被发展更深入的参数化方案来提升模拟效果。

关键词： CLM5 叶面积指数植被总初级生产力西南地区

后配额时代提升我国针织服装对外贸易竞争力的对策

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针织工业 2007年第9期 64-66页

作者：王丽焕徐先林天津工业大学天津300160

后配额时代在给我国针织服装行业带来新的发展机遇的同时,也使其面临巨大的挑战。针对后配额时代世界针织服装贸易格局发生的重大变化,从国家政府、针织服装行业和针织服装企业3个不同的角度进行对策分析,提出在后配额时代,我国应发挥... 详细信息

后配额时代在给我国针织服装行业带来新的发展机遇的同时,也使其面临巨大的挑战。针对后配额时代世界针织服装贸易格局发生的重大变化,从国家政府、针织服装行业和针织服装企业3个不同的角度进行对策分析,提出在后配额时代,我国应发挥自我优势,采取正确竞争战略,全面提升我国针织服装对外贸易的竞争力。

关键词：后配额时代针织服装对外贸易竞争力对策分析