检索结果-南通市图书馆

A direct,sensitive and high-throughput genus and species-specific molecular assay for large-scale malaria screening

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Infectious Diseases of Poverty 2022年第2期11卷 11-22页

作者： Yaling Zhao Ye Zhao Yu Sun lihua fan Duoquan Wang Heng Wang Xiaodong Sun Zhi Zheng Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic MedicinePeking Union Medical CollegeBeijingChina Yunnan Institute of Parasitic Diseases Yunnan Center for Malaria ResearchInstitute of Vector and Pathogen BiologyDali UniversityPuerYunnanChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionWHO Collaborating Center for MalariaSchistosomiasisand FilariasisKey Laboratory of Parasite and Vector BiologyMinistry of HealthShanghaiChina Blood Transfusion Department Peking Union Medical College HospitalChinese Academy of Medical SciencesBeijingChina

Background:Infectious disease diagnostics often requires sensitive molecular assays that identify at both genus and species levels.For large scale screening,such as malaria screening for elimination,diagnostic assay can be a challenge,as both the throughput and cost of the assay must be considered.The requirement of nucleic acid extraction hampers the throughput of most molecular assays.Co-amplification of multiple species or multiplex identification either can result in missed diagnosis or are too costly for large-scale screening.A genus-and species-specific diagnostic assay with simplified procedure,high sensitivity and throughput is still needed.This study aimed to develop a sensitive and high-throughput approach for large-scale infectious disease screening.Methods:We developed multi-section Capture and Ligation Probe PCR(mCLIP-PCR)for the direct detection of RNA without extraction and reverse transcription.Multiple tailed sandwich hybridization probes were used to bind at genus-and species-specific sections of the target RNA to cooperatively capture the target onto a 96-well plate.After enzymatic ligation of the bound probes,a single-stranded DNA formed at each section with distinct tail sequence at the ends.They were separately PCR-amplified with primers corresponding to tail sequences for genus or species identification.We applied the method to the active screening ofPlasmodium infections of 4,580 asymptomatic dried blood spot samples collected in malaria endemic areas and compared the results with standard qPCR using linear regression.Results:With multi-section cooperative capture but separate amplification strategy,we accurately identified genusPlasmodium and speciesP.falciparum andP.vivax without RNA extraction,with favorable sensitivities among the published reports.In the active screening,our method identified all 53 positive infections including two mixed infections,and twoP.vivax infections that were missed by standard qPCR.Conclusions:mCLIP-PCR provides a sensitive and high-throughput approach to la

关键词： Infectious disease Malaria Molecular screening CLIP-PCR Genus Species High-throughput

Melatonin decreases GSDME mediated mesothelial cell pyroptosis and prevents peritoneal fibrosis and ultrafiltration failure

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第2期67卷 360-378页

作者： Hongxia Ruan Xuejuan Li Lina Zhou Zihan Zheng Rulin Hua Xu Wang Yuan Wang Yujie fan Shuwen Guo lihua Wang Shafiq ur Rahman Ziwei Wang Yuyuan Wei Shuangyan Yu Rongzhi Zhang Qian Cheng Jie Sheng Xue Li Xiaoyan Liu Ruqiang Yuan Xiaoyan Zhang Lihong Chen Guowang Xu Youfei Guan Jing Nie Hongqiang Qin Feng Zheng Advanced Institute for Medical Sciences Dalian Medical UniversityDalian 116044China Department of Nephrology Second HospitalDalian Medical UniversityDalian 116023China CAS Key Laboratory of Separation Science for Analytical Chemistry Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China Chongqing International Institute for Immunology Chongqing 401320China Department of Nephrology Shenzhen HospitalSouthern Medical UniversityShenzhen 518101China Wuhu Hospital and Health Science Center East China Normal UniversityShanghai 200241China Peking University First Hospital Peking UniversityBeijing 100034China

Peritoneal fibrosis together with increased capillaries is the primary cause of peritoneal dialysis failure.Mesothelial cell loss is an initiating event for peritoneal fibrosis.We find that the elevated glucose concentrations in peritoneal dialysate drive mesothelial cell pyroptosis in a manner dependent on caspase-3 and Gasdermin E,driving downstream inflammatory responses,including the activation of macrophages.Moreover,pyroptosis is associated with elevated vascular endothelial growth factor A and C,two key factors in vascular angiogenesis and lymphatic vessel formation.GSDME deficiency mice are protected from high glucose induced peritoneal fibrosis and ultrafiltration failure.Application of melatonin abrogates mesothelial cell pyroptosis through a MT1R-mediated action,and successfully reduces peritoneal fibrosis and angiogenesis in an animal model while preserving dialysis efficacy.Mechanistically,melatonin treatment maintains mitochondrial integrity in mesothelial cells,meanwhile activating m TOR signaling through an increase in the glycolysis product dihydroxyacetone phosphate.These effects together with quenching free radicals by melatonin help mesothelial cells maintain a relatively stable internal environment in the face of high-glucose stress.Thus,Melatonin treatment holds some promise in preserving mesothelium integrity and in decreasing angiogenesis to protect peritoneum function in patients undergoing peritoneal dialysis.

关键词： melatonin mitochondria pyroptosis GSDME peritoneal fibrosis mTOR

Verification of Bacteroidales 16S rRNA markers as a complementary tool for detecting swine fecal pollution in the Yangtze Delta

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2020年第4期32卷 59-66页

作者： lihua fan Xiaofeng Zhang Ruoxue Zeng Suhua Wang Chenchen Jin Yongqiang He Jiangbing Shuai Department of Food Science and Nutrition Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310016China Zhejiang Academy of Science and Technology for Inspection and Quarantine Hangzhou 310058China

To correctly assess and properly manage the public health risks associated with exposure to contaminated water,it is necessary to identify the source of fecal pollution in a watershed.In this study,we evaluated the efficacy of our two previously developed real time-quantitative PCR(qPCR)assays for the detection of swine-associated Bacteroidales genetic markers(gene 1-38,gene 3-53)in the Yangtze Delta watershed of southeastern China.The results indicated that the gene 1-38 and 3-53 markers exhibited high accuracy(92.5%,91.7%conditional probability,respectively)in detecting Bacteroidales spp.in water samples.According to binary logistic regression(BLR),these two swine-associated markers were well correlated(P<0.05)with fecal indicators(Escherichia coli and Enterococci spp.)and zoonotic pathogens(E.coli O157:H7,Salmonella spp.and Campylobacter spp.)in water samples.In contrast,concentrations of conventional fecal indicator bacteria(FIB)were not correlated with zoonotic pathogens,suggesting that they are noneffective at detecting fecal pollution events.Collectively,the results obtained in this study demonstrated that a swinetargeted qPCR assay based on two Bacteroidales genes markers(gene 1-38,gene 3-53)could be a useful tool in determining the swine-associated impacts of fecal contamination in a watershed.

关键词： Bacteroidales genes markers Water pollution Real-time quantitative PCR Fecal indicators Zoonotic pathogens

Traumatic extracranial internal carotid-jugular fistula leading to serious injury: a case report in forensic assessment

在线全文

学校读者我要写书评

暂无评论

Forensic Sciences Research 2020年第2期5卷 170-173页

作者： Xia Liu lihua fan Chongliang Ying Yibin Cheng Maowen Wang Department of Clinical Forensic Medicine Shanghai Key Laboratory of Forensic MedicineShanghai Forensic PlatformAcademy of Forensic ScienceShanghaiChina

Extracranial carotid artery injuries may produce severe haemorrhage,cerebral damage or arteriovenous fistula.Examples of traumatic extracranial carotid-jugular fistula are not frequently reported,especially in forensic medicine.We report a controversial case of an extracranial internal carotid-jugular fistula resulting from a stab wound to the neck.The degree of the injury was classified under“The Standard of Human Body Injury Assessment(2014)”(SIA)in China by forensic examiners.We believe this case report will provide information for the forensic assessment of similar cases.

关键词： Forensic sciences carotid artery trauma extracranial internal carotid-jugular fistula injury degree assessment

Cr/Mo/W元素对镍基高温合金γ/γ′共格组织热稳定性的影响

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

金属学报 2024年第4期60卷 453-463页

作者：凡莉花李金临孙九栋吕梦甜王清董闯大连理工大学材料科学与工程学院三束材料改性教育部重点实验室大连116024

近年来,随着航空航天发动机的持续发展,对其关键热端部件的承温能力要求越来越高,而高温合金共格组织热稳定性是影响其力学性能的关键因素,本工作在团簇成分式[Al-Ni_(12)](Al_(1)(Ti,Nb,Ta)_(0.5)(Cr,Mo,W)_(1.5))基础上,保持其他元素... 详细信息

近年来,随着航空航天发动机的持续发展,对其关键热端部件的承温能力要求越来越高,而高温合金共格组织热稳定性是影响其力学性能的关键因素,本工作在团簇成分式[Al-Ni_(12)](Al_(1)(Ti,Nb,Ta)_(0.5)(Cr,Mo,W)_(1.5))基础上,保持其他元素含量不变,改变Cr、Mo、W的含量设计了系列镍基高温合金(含Cr_(1.0)Mo_(0.5)的S1-CM、Cr_(1.0)W_(0.5)的S2-CW和含Cr_(0.7)Mo_(0.4)W_(0.4)的S3-CMW),研究Cr、Mo、W含量变化对合金γ/γ′共格组织热稳定性的影响。采用非自耗真空电弧炉制备合金铸锭,并对其在1300℃固溶15 h后在900℃下进行长期时效处理,并对时效态样品进行微观组织表征和力学性能测试。结果表明,这3个合金的微观组织均表现为高体积分数(f>70%)的γ′粒子均匀地分布在fcc-γ基体中。其中,S1-CM和S2-CW合金在900℃、50 h时效后γ′粒子形貌均呈椭球形,而S3-CMW合金则表现为方形,这是由于后者具有更负的γ/γ′点阵错配度(后者δ=-0.47%,前者δ=-0.25%~-0.33%)。500 h长期时效后,各合金中的γ′粒子形貌没有发生变化,且都具有较慢的粗化速率(K=10~18 nm^(3)/s);尤其S3-CMW合金表现出最高的γ/γ′共格组织稳定性(γ′粒子的粗化速率K=10.02 nm^(3)/s),且晶界处的第二相粒子析出明显低于其他合金。系列合金的硬度基本不随时效时间发生变化,也进一步表明了共格组织的稳定性;其中S3-CMW合金的显微硬度为397~418 HV,时效200 h后的室温压缩屈服强度为818 MPa。

关键词：镍基高温合金共格组织 γ′粒子粗化第二相析出

Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023 edition)

在线全文

学校读者我要写书评

暂无评论

Chinese Herbal Medicines 2024年第1期16卷 3-12页

作者： Qing Zhao Tong Wang Hongbin Wang Peng Cao Chengyu Jiang Hongzhi Qiao lihua Peng Xingdong Lin Yunyao Jiang Honglei Jin Huantian Zhang Shengpeng Wang Yang Wang Ying Wang Xi Chen Junbing fan Bo Li Geng Li Bifeng Liu Zhiyang Li Suhua Qi Mingzhen Zhang Jianjian Zheng Jiuyao Zhou Lei Zheng Kewei Zhao Guangdong Engineering Research Center of Chinese Herbal Vesicles The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis The Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Institute of Life and Health Engineering the First Affiliated HospitalJinan UniversityGuangzhou 510630China School of Pharmaceutical Sciences Guangzhou University of Chinese MedicineGuangzhou 510006China College of Pharmacy Nanjing University of Chinese MedicineNanjing 210046China Institute of Basic Medicine Chinese Academy of Medical SciencesBeijing 100005China College of Pharmaceutical Sciences Zhejiang UniversityHangzhou 310030China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China Department of Bone and Joint Surgery The First Affiliated Hospital of Jinan UniversityGuangzhou 510630China State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoAomen 999078China Shanghai Jiao Tong University Affiliated Sixth People’s Hospital Shanghai 200233China School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515China School of Life Sciences Nanjing UniversityNanjing 210008China Nanfang Hospital Southern Medical UniversityGuangzhou 510515China Laboratory Animal Center Guangzhou University of Chinese MedicineGuangzhou 510405China College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China Nanjing University Medical School Affiliated Nanjing Drum Tower Hospital Nanjing 210008China School of Medical and Technology Xuzhou Medical UniversityXuzhou 221004China School of Basic Medical Sciences Xi’an Jiaotong UniversityXi’an 710065China The First Affiliated Hospital of Wenzhou Medical University WenZhou 325035China

To promote the development of extracellular vesicles of herbal medicine especially the establishment of standardization,led by the National Expert Committee on Research and Application of Chinese Herbal Vesicles,research experts in the field of herbal medicine and extracellular vesicles were invited nationwide with the support of the Expert Committee on Research and Application of Chinese Herbal Vesicles,Professional Committee on Extracellular Vesicle Research and Application,Chinese Society of Research Hospitals and the Guangdong Engineering Research Center of Chinese Herbal Vesicles.Based on the collation of relevant literature,we have adopted the Delphi method,the consensus meeting method combined with the nominal group method to form a discussion draft of“Consensus statement on research and application of Chinese herbal medicine derived extracellular vesicles-like particles(2023)”.The first draft was discussed in online and offline meetings on October 12,14,November 2,2022 and April and May 2023 on the current status of research,nomenclature,isolation methods,quality standards and research applications of extracellular vesicles of Chinese herbal medicines,and 13 consensus opinions were finally formed.At the Third Academic Conference on Research and Application of Chinese Herbal Vesicles,held on May 26,2023,Kewei Zhao,convenor of the consensus,presented and read the consensus to the experts of the Expert Committee on Research and Application of Chinese Herbal Vesicles.The consensus highlights the characteristics and advantages of Chinese medicine,inherits the essence,and keeps the righteousness and innovation,aiming to provide a reference for colleagues engaged in research and application of Chinese herbal vesicles at home and abroad,decode the mystery behind Chinese herbal vesicles together,establish a safe,effective and controllable accurate Chinese herbal vesicle prevention and treatment system,and build a bridge for Chinese medicine to the world.

关键词： Chinese herbal medicine extracellular vesicles research application consensus

在线全文

学校读者我要写书评

暂无评论

无线射频压裂滑套系统模拟试验研究

石油机械 2024年第4期52卷 27-32页

作者：张利巍孙露凯范立华东北石油大学物理与电子工程学院大庆油田井下作业分公司

将无线射频识别(RFID)技术应用到油水井压裂中,能够很好地解决现有压裂滑套存在的压裂级数受限、操作非智能等诸多问题。因井下工况复杂,理论分析与实际应用差别较大,为进一步系统地探究井下无线射频系统的开发应用,使用三维软件设计无... 详细信息

将无线射频识别(RFID)技术应用到油水井压裂中,能够很好地解决现有压裂滑套存在的压裂级数受限、操作非智能等诸多问题。因井下工况复杂,理论分析与实际应用差别较大,为进一步系统地探究井下无线射频系统的开发应用,使用三维软件设计无线射频滑套模拟试验台,对标签的运动状态进行理论分析,并建立室内模拟试验平台,系统地进行井下相关试验。试验结果表明:标签选择有铁氧体磁芯,且天线绕制形式选用单层螺线管线圈,可以提高整个系统的性能;在系统电感值相同的情况下,单层螺线管天线轴向长度对阅读器模块能识别的标签最大通过速度有积极的影响;标签天线线圈与阅读器天线线圈平面之间的角度也会影响标签的识别率。研究结果对井下无线射频系统的开发应用具有一定的指导作用。

关键词：油水井压裂滑套无线射频识别标签试验台天线

维普期刊数据库博看期刊

针刺水通、水金穴对全身麻醉诱导期舒芬太尼诱发呛咳的影响:随机对照试验

在线全文

学校读者我要写书评

暂无评论

中国针灸 2024年第1期44卷 62-66页

作者：许际平季加富胡丽华厉朋苏帆日照市中心医院麻醉科山东日照276800 山东中医药大学附属医院麻醉科济南250014

目的:观察针刺水通、水金穴预防全身麻醉诱导期舒芬太尼诱发呛咳反应的疗效及对血流动力学的影响。方法:将80例在全身麻醉下行择期手术患者,随机分为观察组(40例)和对照组(40例,剔除1例)。对照组给予常规麻醉诱导,静脉注射1%枸橼酸舒芬... 详细信息

目的:观察针刺水通、水金穴预防全身麻醉诱导期舒芬太尼诱发呛咳反应的疗效及对血流动力学的影响。方法:将80例在全身麻醉下行择期手术患者,随机分为观察组(40例)和对照组(40例,剔除1例)。对照组给予常规麻醉诱导,静脉注射1%枸橼酸舒芬太尼注射液0.5μg/kg、1%丙泊酚注射液(总剂量按照2 mg/kg计算)、苯磺顺阿曲库铵注射液0.2 mg/kg;观察组在常规麻醉诱导前针刺右侧水通、水金穴,留针30 min。观察麻醉诱导期间两组患者呛咳、胸壁僵硬并发症情况,记录患者进入手术室平躺5 min后(T_(0))、静脉注射枸橼酸舒芬太尼注射液即刻(T_(1))及注射后2 min(T_(2))、插管前1 min(T_(3))、插管1 min后(T_(4))的收缩压(SBP)、心率(HR)、脉搏血氧饱和度(SpO_(2))。结果:麻醉诱导期,观察组患者轻、中、重度呛咳情况优于对照组(P0.05)。两组患者T_(0)、T_(1)、T_(2)、T_(3)、T_(4)时SBP、HR、SpO_(2)比较,差异无统计学意义(P>0.05)。与T_(1)时比较,对照组T_(2)时SBP、HR升高(P0.05),观察组T_(2)时SBP、HR、SpO_(2)差异无统计学意义(P>0.05)。结论:针刺水通、水金穴可以有效预防舒芬太尼引起的呛咳反应,降低呛咳严重程度,使血流动力学更加平稳。

关键词：全身麻醉麻醉诱导咳嗽胸壁僵硬针刺舒芬太尼水通穴水金穴

间充质干细胞治疗新型冠状病毒肺炎的机制及研究进展

在线全文

学校读者我要写书评

暂无评论

中国免疫学杂志 2024年第1期40卷 220-224页

作者：凌小穗何海萍李凡张丽华昆明理工大学医学院昆明650500 华东师范大学附属芜湖医院芜湖241060

新型冠状病毒肺炎(COVID-19)感染性强,且重型及危重型患者病死率高,缺乏特异性治疗方法,急需寻找安全有效的治疗方法。间充质干细胞(MSCs)具有免疫调节、组织修复、再生、迁移和归巢、抗病毒和抗炎等生物学特性。目前应用MSCs治疗COVID... 详细信息

新型冠状病毒肺炎(COVID-19)感染性强,且重型及危重型患者病死率高,缺乏特异性治疗方法,急需寻找安全有效的治疗方法。间充质干细胞(MSCs)具有免疫调节、组织修复、再生、迁移和归巢、抗病毒和抗炎等生物学特性。目前应用MSCs治疗COVID-19的研究已逐步开展。本文就2019新型冠状病毒(2019-nCoV)的致病机制、MSCs治疗COVID-19的潜在机制及面临的挑战进行综述。

关键词：间充质干细胞新型冠状病毒肺炎临床试验