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Journal of Integrative Plant Biology 2021年第10期63卷 1724-1739页

作者： Xinyong Liu Xichun Zhang Ruijie Cao Guiai Jiao Shikai Hu Gaoneng Shao Zhonghua Sheng lihong Xie Shaoqing Tang Xiangjin Wei Peisong Hu State Key Laboratory of Rice Biology China National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 310006China Guizhou Rice Research Institute Guizhou Academy of Agricultural SciencesGuiyang 550006China

Pentatricopeptide repeat(PPR)proteins play important roles in the post-transcriptional modification of organellar RNAs in ***,the function of most PPR proteins remains ***,we characterized the rice(Oryza sativa L.)chlorophyll deficient 4(cde4)mutant which exhibits an albino phenotype during early leaf development,with decreased chlorophyll contents and abnormal chloroplasts at low-temperature(20℃).Positional cloning revealed that CDE4 encodes a P-type PPR protein localized in *** the cde4 mutant,plastid-encoded polymerase(PEP)-dependent transcript levels were significantly reduced,but transcript levels of nuclear-encoded genes were increased compared to wild-type plants at 20℃.CDE4 directly binds to the transcripts of the chloroplast genes rpl2,ndhA,and *** splicing of these transcripts was defective in the cde4 mutant at 20℃,but was normal at 32℃.Moreover,CDE4 interacts with the guanylate kinase vIRESCENT 2(v2);overexpression of v2 enhanced CDE4 protein stability,thereby rescuing the cde4 phenotype at 20℃.Our results suggest that CDE4 participates in plastid RNA splicing and plays an important role in rice chloroplast development under lowtemperature conditions.

关键词： CDE4 chloroplast development pentatricopeptide repeat(PPR)protein RNA splicing low-temperature Oryza sativa L.

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采矿权安全性、政府规制与企业绿色技术创新

中国人口·资源与环境 2021年第4期31卷 96-107页

作者：王艳于立宏华东理工大学商学院上海200237

采矿权安全性是影响采矿企业投资行为的关键因素。文章聚焦采矿权安全性对企业绿色技术创新行为的影响,通过演化博弈模型和L-v模型,分析了采矿权安全性影响下,政府规制行为选择与采矿企业绿色技术创新行为的动态演化过程,进而采用2019... 详细信息

采矿权安全性是影响采矿企业投资行为的关键因素。文章聚焦采矿权安全性对企业绿色技术创新行为的影响,通过演化博弈模型和L-v模型,分析了采矿权安全性影响下,政府规制行为选择与采矿企业绿色技术创新行为的动态演化过程,进而采用2019年中国采矿权交易数据进行了仿真模拟。结果发现:①当采矿权安全性较低时,其对采矿企业的绿色技术创新具有倒逼作用,但随着研发投入的增加和研发周期的增长,企业的绿色技术创新动力不断降低。②当采矿权安全性较高时,研发投入过高或过低均难以激励采矿企业开展绿色技术创新,但是,研发投入越低,政府越倾向于采用规制策略。③当采矿权安全性处于中等状态时,企业演化稳定策略与研发投入有关,而政府的演化稳定策略为规制。④绿色技术创新增长率具有促进采矿企业绿色技术创新的作用,增长率越高,采矿企业对绿色研发投入的预期收益越高,绿色创新意愿也越强。⑤采矿权期限限制了技术影响因子的作用。受私有企业绿色技术创新的影响,只有在中型矿权中,国有企业才具备更强的创新能力和创新积极性,而在大型矿权和小型矿权中,国有企业不具备绿色技术创新优势。基于以上结论,研究认为,政府在以政策手段引导采矿企业的绿色技术创新时,应该强化绿色技术水平在矿业权竞争性出让中的作用,并结合不同规模与类型的采矿权特征深化矿业权制度改革;通过营造采矿业绿色创新环境,加强政府配套政策的协调与配合,提高多种政策的协同有效性,推动采矿业绿色技术水平的提高和生态环境保护。

关键词：采矿权安全性政府规制绿色技术创新

植物乳杆菌LP21绿色合成纳米硒及对溶藻弧菌的抑菌活性

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食品科学 2022年第2期43卷 217-223页

作者：王丽红杨辉苏文孔阳陕西科技大学食品与生物工程学院陕西西安710021

采用益生菌植物乳杆菌LP21合成纳米硒,为抑制水产养殖中溶藻弧菌感染提供一种新的方法。通过植物乳杆菌LP21绿色合成纳米硒,对所得纳米硒进行透射电镜形貌及能谱分析,粒径仪测定粒径,红外光谱分析纳米硒表面生物分子的分布,并研究纳米... 详细信息

采用益生菌植物乳杆菌LP21合成纳米硒,为抑制水产养殖中溶藻弧菌感染提供一种新的方法。通过植物乳杆菌LP21绿色合成纳米硒,对所得纳米硒进行透射电镜形貌及能谱分析,粒径仪测定粒径,红外光谱分析纳米硒表面生物分子的分布,并研究纳米硒对溶藻弧菌的抑菌活性。结果表明,植物乳杆菌LP21还原的纳米硒呈球形,分散性良好;纯化的纳米硒平均尺寸为184.6 nm,其表面包裹蛋白、多糖等生物分子,呈无定形态;纳米硒对溶藻弧菌、大肠杆菌的抑菌活性较强,对溶藻弧菌的最小抑菌浓度为286μg/mL,在亚抑制剂量下对溶藻弧菌的生长、运动性、生物膜有显著抑制,并引起菌体细胞形态发生变化,此抑菌研究为纳米硒在水产养殖业感染菌防治中的应用提供依据。

关键词：植物乳杆菌纳米硒溶藻弧菌结构表征抑菌活性

Coulomb effects in the Ge/Si Single Quantum Dot

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Coulomb effects in the Ge/Si Single Quantum Dot

Succinate dehydrogenase SDH1–1 positively regulates cotton resistance to verticillium dahliae through a salicylic acid pathway

2012 China Functional Materials Technology and Industry Forum

作者： lihong Zhang Chong wang Jie Yang Jintao Yao Yu Yang Institute of Optoelectronic Information Materials Yunnan University

Using scanning probe microscopy(SPM) technique,the electronic properties of Ge/Si quantum dots(QDs) have been *** results demonstrate that a layer of a disordered structure is formed between the Ge/Si QDs and the surface of Si substrate due to the defects in QDs during the bias voltage *** is,a double tunneling system in which the Coulomb blocking effect can be observed is constructed during the electronic measurement for the single quantum dot (SQD).

关键词： Ge/Si single QD I-v curve Coulomb characteristics

来源： cnki会议评论

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Journal of Cotton Research 2020年第2期3卷 103-114页

作者： ZHANG Xiangyue FENG Zili ZHAO lihong LIU Shichao WEI Feng SHI Yongqiang FENG Hongjie ZHU Heqin State Key Laboratory of Cotton Biology Institute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000HenanChina Zhengzhou Research Base State Key Laboratory of Cotton BiologySchool of Agricultural SciencesZhengzhou UniversityZhengzhou 450001China.

Background:verticillium wilt,caused by the soil-borne fungus of verticillium dahliae Kleb.,is one of the most devastating diseases of *** complex mechanism underlying cotton resistance to verticillium wilt remains *** an endogenous resistance gene may be helpful to control this *** studies revealed that succinate dehydrogenase(SDH)is involved in reactive oxygen species(ROS)-induced stress signaling pathway that is likely to be triggered by salicylic acid(SA).Here,through the metabolomics and differential expression analyses in wilt-inoculated cotton(Gossypium hirsutum),we noticed that Gh SDH1–1 gene in cotton may play an important role in the resistance to *** we reported Gh SDH1–1 gene and its functional analysis in relation to the resistance of cotton to ***:The Gh SDH1–1 gene in cotton root was significantly up-regulated after *** inoculation,and its expression level peaked at 12 and 24 h *** can also induce the up-regulation of Gh SDH1–***,the functional analysis showed that Gh SDH1–1-silenced cotton was more susceptible to *** than the control because of the significant decrease in abundance of immune-related molecules and severe damage to the SA-signaling *** Arabidopsis thaliana,high expression of Gh SDH1–1 conferred high resistance to *** that overexpressed Gh SDH1–1 had higher resistance to *** infection compared with the ***:Our findings provide new insights into the role of Gh SDH1–1;it positively regulates cotton resistance to verticillium *** regulatory mechanism of Gh SDH1–1 is closely related to SA-related signaling pathway.

关键词： Cotton verticillium wilt Resistance gene GhSDH1–1 Salicylic acid

Electrostatic gating of solid-ion-conductor on InSe flakes and InSe/h-BN heterostructures

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Chinese Physics B 2020年第11期29卷 543-547页

作者： Zhang Zhou Liangmei Wu Jiancui Chen Jiajun Ma Yuan Huang Chengmin Shen lihong Bao Hong-Jun Gao Institute of Physics Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences&CAS Center for Excellence in Topological Quantum Computation Chinese Academy of SciencesBeijing 100190China Songshan Lake Materials Laboratory Dongguan 523808China

We report the electrical transport properties of InSe flakes electrostatically gated by a solid ion *** large tuning capability of the solid ion conductor as gating dielectric is confirmed by the saturation gate voltage as low as^1 v and steep subthreshold swing(83 mv/dec).The p-type conduction behavior of InSe is obtained when negative gate voltages are *** doping of the solid ion conductor is suppressed by inserting a buffer layer of hexagonal boron nitride(h-BN)between InSe and the solid-ion-conductor *** comparing the performance of devices with and without h-BN,the capacitance of solid ion conductors is extracted to be the same as that of^2 nm h-BN,and the mobility of InSe on solid ion conductors is comparable to that on the SiO2 *** results show that solid ion conductors provide a facile and powerful method for electrostatic doping.

关键词： solid ion conductors electrostatic gating InSe van der Waals heterostructure

A review of the pathogenicity mechanism of verticillium dahliae in cotton

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Journal of Cotton Research 2022年第1期5卷 59-71页

作者： ZHANG Yalin ZHOU Jinglong ZHAO lihong FENG Zili WEI Feng BAI Hongyan FENG Hongjie ZHU Heqin State Key Laboratory of Cotton Biology Institute of Cotton Research of Chi-nese Academy of Agricultural SciencesAnyang 455000HenanChina West-ern Agricultural Research Center of Chinese Academy of Agricultural Sciences Changji 831100XinjiangChina Engineering Research Centre of Cotton Ministry of Education/College of AgricultureXinjiang Agricultural UniversityUrumqi 830052XinjiangChina

verticillium wilt,caused by the notorious fungal pathogen verticillium dahliae,is one of the main limiting factors for cotton *** to the stable dormant structure microsclerotia,long-term variability and co-evolution with host plant,its pathogenicity mechanism is very complicated,and the interaction mechanism between pathogen and host plant is also *** identification and functional analysis of the genes involved in the pathogenicity or virulence of this fungus will benefit to uncover the molecular pathogenic mechanism of *** this review,many multifunction genes covering microsclerotia development,pathogen infection,effector proteins,transcription factors,horizontal gene transfer and trans-kingdom RNA silencing have been summarized to provide a theoretical basis to deep understand the molecular pathogenicity mechanism of *** and promote to effectively control verticillium ***,these pathogenicity-related genes may be considered as targets for effective control of verticillium wilt in cotton.

关键词： verticillium dahliae Cotton Pathogenicity-related genes Molecular pathogenic mechanism

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脱绒棉种介电分选参数的优化

农业工程学报 2010年第9期26卷 114-119页

作者：坎杂谷趁趁王丽红付威石河子大学机械电气工程学院石河子832003 新疆兵团农业机械重点实验室石河子832003

为优化脱绒棉种介电分选参数,采用二次回归通用旋转组合设计方法,建立介电分选后获选棉种的介电常数、棉种电导率和棉种活力指数分别与分选电压、滚筒转速和棉种含水率3个主要的介电分选影响因素之间关系的数学模型,分析各影响因素对各... 详细信息

为优化脱绒棉种介电分选参数,采用二次回归通用旋转组合设计方法,建立介电分选后获选棉种的介电常数、棉种电导率和棉种活力指数分别与分选电压、滚筒转速和棉种含水率3个主要的介电分选影响因素之间关系的数学模型,分析各影响因素对各试验指标的影响,并进行参数优化,确定各影响因素的参数组合为:分选电压6000～6574.43v,滚筒转速30.08～34.38r/min,棉种含水率为4%。

关键词：介电特性优化数学模型脱绒棉种

一款0.16 mm^(2)基于180 nm CMOS采用全局去偏斜的半速率8×2.5 Gb/s时钟转发架构接收机

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集成电路与嵌入式系统 2024年第4期24卷 1-9页

作者：杨力宏李世新韩晨曦云越恒刘术彬赵潇腾朱樟明西安电子科技大学西安710071

在时钟转发架构的高速有线通信接收机中,需要去偏斜电路实现时钟与数据之间的最佳采样关系,并保证多路数据的同步。本文提出了一种全局去偏斜方案,仅采用一路数据与时钟进行对齐,并通过时钟延时匹配与分布技术实现多路数据同步,减小了... 详细信息

在时钟转发架构的高速有线通信接收机中,需要去偏斜电路实现时钟与数据之间的最佳采样关系,并保证多路数据的同步。本文提出了一种全局去偏斜方案,仅采用一路数据与时钟进行对齐,并通过时钟延时匹配与分布技术实现多路数据同步,减小了各通道独立去偏斜方案带来的功耗与面积开销。所提出的接收机由8路数据通道、1路半速率转发时钟通道与基于延迟锁定环路的全局去偏斜电路构成。基于180 nm CMOS工艺,在2.5 Gb/s数据率下,可去除输入时钟与数据任意偏斜,得到位于数据中心的采样相位,同时具有时钟占空比校准能力。在1.8 v电源电压下,所提出的接收机总功耗为187 mW,总面积为0.16 mm^(2),对比各通道独立去偏斜方案,功耗和面积开销分别节约了45.2%与62.8%。

关键词：时钟转发多路接收机全局去偏斜延迟锁定环路时钟分布数据同步半速率

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新生儿嗜肺军团菌肺炎1例并文献复习

中华新生儿科杂志（中英文） 2024年第5期39卷 289-293页

作者：付芬芬华敏敏张丽平霍婉莹雷冰尚利宏郑州大学第三附属医院新生儿科郑州450052

目的探讨新生儿嗜肺军团菌肺炎的临床特点。方法对郑州大学第三附属医院新生儿科收治的1例嗜肺军团菌肺炎新生儿临床资料进行回顾性分析,以“嗜肺军团菌”、“新生儿”、“早产儿”、“Legionella pneumophila”、“Legionnaires'diseas... 详细信息

目的探讨新生儿嗜肺军团菌肺炎的临床特点。方法对郑州大学第三附属医院新生儿科收治的1例嗜肺军团菌肺炎新生儿临床资料进行回顾性分析,以“嗜肺军团菌”、“新生儿”、“早产儿”、“Legionella pneumophila”、“Legionnaires'disease”、“neonate”、“infant”、“newborn”为关键词,对中国知网、万方、维普、中华医学期刊全文数据库、PubMed、Web of science、Embase数据库自建库至2023年6月收录的文献进行检索,总结新生儿嗜肺军团菌肺炎的临床特点。结果本例患儿为足月男婴,表现为呛咳、发热、呼吸窘迫、感染性休克,CT示肺实变、空洞,常规抗感染治疗无效,经纤维支气管镜诊断先天性食管闭锁(Ⅴ型),送检肺泡灌洗液宏基因二代测序确诊嗜肺军团菌肺炎,予阿奇霉素联合左氧氟沙星抗感染、手术修补气管食管瘘等治疗后好转出院,随访至10月龄,未遗留后遗症。经文献检索共纳入19篇新生儿嗜肺军团菌肺炎文献,加上本例共31例,起病时表现为发热24例,呼吸窘迫22例,纳差、嗜睡11例,咳嗽5例;主要并发症为呼吸衰竭20例,脓毒症19例,感染性休克16例,多脏器功能衰竭13例,持续性低钠血症6例,抽搐2例,颅内出血、深静脉血栓各1例。30例描述了肺部影像学检查结果,24例肺炎伴实变,6例囊性变、空洞形成,3例坏死性肺炎,2例合并先天性食管闭锁Ⅴ型,1例肺纤维化。经呼吸道分泌物聚合酶链反应确诊24例、培养阳性5例;血清嗜肺军团菌抗体、尿嗜肺军团菌抗原阳性各12例,宏基因二代测序检出嗜肺军团菌3例;尸检发现肺组织嗜肺军团菌阳性3例。死亡7例,24例好转出院,预后良好。结论新生儿嗜肺军团菌肺炎临床表现无特异性,病情常进展迅速,救治不及时病死率高,新生儿肺炎常规抗生素治疗无效时需考虑嗜肺军团菌感染可能。

关键词：嗜肺军团菌肺炎新生儿早产儿