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细菌介导重金属转化过程及金属组学研究方法

中国无机分析化学 2024年第1期14卷 26-34页

作者：唐寅寅刘丽红何滨胡立刚中国科学院生态环境研究中心环境化学与生态毒理学国家重点实验室北京100085 中国科学院大学北京100049 国科大杭州高等研究院环境学院杭州310024

重金属污染依然是我国严重的环境问题之一。在重金属胁迫下,微生物可通过复杂的过程,对重金属进行转化,降低重金属的毒性,其中转化涉及的分子机制广受关注。目前,在上述机制相关研究中,对转化过程中重金属的衡量多局限于总量的测定,而... 详细信息

重金属污染依然是我国严重的环境问题之一。在重金属胁迫下,微生物可通过复杂的过程,对重金属进行转化,降低重金属的毒性,其中转化涉及的分子机制广受关注。目前,在上述机制相关研究中,对转化过程中重金属的衡量多局限于总量的测定,而对其赋存形态的研究不足,导致难以取得有效进展。对细菌介导重金属的转化过程及金属在其中的赋存形态进行综述,探讨了金属组学研究方法在其中的应用,重点针对重金属的颗粒态与蛋白质结合态进行分析、表征和鉴定,为微生物介导重金属的转化研究提供新视角。

关键词：重金属污染金属组学转化细菌介导

Amorphous FeCoNi-S as efficient bifunctional electrocatalysts for overall water splitting reaction

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Chinese Chemical Letters 2023年第2期34卷 576-581页

作者： Runze he Chunyan Wang ligang Feng School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China

Developing bifunctional electrocatalysts for overall water splitting reaction is still highly desired but with large challenges. herein, an amorphous Fe Co Ni-S electrocatalyst was developed using thioacetamide for the sulfuration of Fe Co Ni hydroxide during the hydrothermal process. The obtained catalyst exhibited an amorphous structure with hybrid bonds of metal-S bond and metal-O bonds in the catalyst system. The optimized catalyst showed a largely improved bifunctional catalytic ability to drive water splitting reaction in the alkaline electrolyte compared to the Fe Co Ni hydroxide. It required an overpotential of 280 m V and 80 m V(No-IR correction) to offer 10 m A/cm^(2)for water oxidation and reduction respectively;a low cell voltage of 1.55 V was required to reach 10 m A/cm^(2)for the water electrolysis with good stability for12 h. Moreover, this catalyst system showed high catalytic stability, catalytic kinetics, and Faraday efficiency for water splitting reactions. Considering the very low intrinsic activity of Fe Co Ni hydroxide, the efficient bifunctional catalytic ability should result from the newly formed hybrid active sites of metallic metal-S species and the high valence state of metal oxide species. This work is effective in the bifunctional catalytic ability boosting for the transition metal materials by facile sulfuration in the hydrothermal approach.

关键词： Bifunctional electrocatalyst Amorphous Overall water splitting FeCoNi hydroxide Sulfuration

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抗寒柑橘新品种‘龟井2501’

园艺学报 2021年第S2期48卷 2813-2814页

作者：何利刚王志静宋放吴黎明蒋迎春彭家清黄咏明刘涛湖北省农业科学院果树茶叶研究所湖北省农业科技创新中心果茶分中心武汉430064 十堰市经济作物研究所湖北十堰442714

柑橘新品种‘龟井2501’是由温州蜜柑‘龟井25’的抗寒芽变选育而成。树势中庸,树冠自然圆头形。果实中等大小,平均单果质量110 g,果形端正,扁圆形,果形指数0.75。果面光滑,果皮薄,橙黄色,易剥离。可溶性固形物含量13.0%,可滴定酸含量0.... 详细信息

柑橘新品种‘龟井2501’是由温州蜜柑‘龟井25’的抗寒芽变选育而成。树势中庸,树冠自然圆头形。果实中等大小,平均单果质量110 g,果形端正,扁圆形,果形指数0.75。果面光滑,果皮薄,橙黄色,易剥离。可溶性固形物含量13.0%,可滴定酸含量0.48%,无核,风味甜酸适度,化渣性好,品质优良。果实10月中旬成熟。抗寒性强,适应性广,优质,丰产性强,适宜在湖北柑橘产区及相似区域种植。

关键词：柑橘抗寒品种

柑橘菌根信号受体蛋白基因LYK2的克隆及功能分析

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园艺学报 2022年第2期49卷 281-292页

作者：宋放李子璇王策王志静何利刚蒋迎春吴黎明白福玺湖北省农业科学院果树茶叶研究所湖北省农业科技创新中心果树茶叶研究分中心武汉430064

从甜橙基因组中鉴定到12个菌根信号受体蛋白基因CsLYK,分布于chr1、chr2、chr6、chr7、chr8和chr9等6条染色体上。结构域分析表明CsLYK家族基因的3′端含有7个保守motif,且其组合也高度保守;而5′端仅有3个保守motif,且其组合变异较大... 详细信息

从甜橙基因组中鉴定到12个菌根信号受体蛋白基因CsLYK,分布于chr1、chr2、chr6、chr7、chr8和chr9等6条染色体上。结构域分析表明CsLYK家族基因的3′端含有7个保守motif,且其组合也高度保守;而5′端仅有3个保守motif,且其组合变异较大。另外,CsLYK2、CsLYK3、CsLYK6、CsLYK10和CsLYK12没有内含子,其余7个成员含有1~19个内含子。系统进化分析将CsLYK基因分为3组,CsLYK2与已经报道的菌根共生信号受体基因PaNFP和SlLYK10处于同一小分支。qRT-PCR分析表明,枳实生苗中12个PtrLYK在根、茎、叶中均有表达,其中PtrLYK10在根系中高量表达,PtrLYK3、PtrLYK7、PtrLYK8、PtrLYK9和PtrLYK12在叶片中高量表达;另外,PtrLYK2在枳根系中的表达受菌根侵染的诱导,且在菌根侵染的早期表达量最高。在拟南芥原生质体中瞬时表达亚细胞定位载体35S-PtrLYK2-CFP,共聚焦荧光显微镜观察发现PtrLYK2定位于细胞膜上。通过苜蓿毛状根瞬时表达启动子表达载体pro PtrLYK2::GUS,利用Ds-Red红色荧光报告基因筛选阳性根并接种AM真菌,通过GUS组织化学染色和WGA-488菌根染色发现pro PtrLYK2主要在丛枝周围的细胞和根尖细胞表达。这些结果表明PtrLYK2可能在柑橘菌根共生早期的信号识别中发挥重要功能。

关键词：柑橘丛枝菌根真菌 LysM受体类似蛋白激酶亚细胞定位启动子

国外橡胶树优良品种RRIM712生产适应性系比试验

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热带农业科技 2017年第1期40卷 1-5页

作者：刘忠亮邱彦芬何树明李芹和丽岗云南省热带作物科学研究所云南景洪666100 云南农业大学热带作物学院云南普洱665000 云南省红河热带农业科学研究所云南河口661300

在云南植胶区对橡胶树优良品种RRIM712的27年生产适应性试验表明:RRIM712生势中等,产量中等,抗辐射型寒害能力差,与对照RRIM600相当;割胶刺激反应中等,死皮病情、刺激类型与RRIM600相近,属一类刺激品种。

关键词：橡胶树 RRIM712 品种适应性

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硬实时任务在多处理机上的动态调度研究

华中科技大学学报（自然科学版） 2001年第5期29卷 68-70页

作者：何黎刚韩宗芬金海高兰华中科技大学计算机科学与技术学院

在硬实时任务集能够在多处理机上进行静态调度的基础上 ,从理论上研究了相同硬实时任务集在多处理机上能够进行动态调度的条件 ,并给出了动态调度规则 ,为硬实时任务的动态调度问题提供了一种新的解决途径 .

关键词：静态调度动态调度多处理机调度规则多机实时任务调度可抢占调度

Nanotwinned and hierarchical nanotwinned metals:a review of experimental,computational and theoretical efforts

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npj Computational Materials 2018年第1期4卷 613-630页

作者： ligang Sun Xiaoqiao he Jian Lu Department of Mechanical and Biomedical Engineering City University of Hong KongTat Chee AvenueKowloonHong Kong Hong Kong Branch of National Precious Metals Material Engineering Research Center City University of Hong KongTat Chee AvenueKowloonHong Kong Department of Architecture and Civil Engineering City University of Hong KongTat Chee AvenueKowloonHong Kong Centre for Advanced Structural Materials City University of Hong KongShenzhen Research Institute8 Yuexing 1st RoadShenzhen Hi-Tech Industrial ParkNanshan DistrictShenzhenChina

The recent studies on nanotwinned(NT)and hierarchical nanotwinned(HNT)face-centered cubic(FCC)metals are presented in this *** HNT structures have been supposed as a kind of novel structure to bring about higher strength/ductility than NT counterparts in crystalline *** primarily focus on the recent developments of the experimental,atomistic and theoretical studies on the NT and HNT structures in the metallic *** advanced bottom-up and top-down techniques for the fabrication of NT and HNT structures are *** deformation induced HNT structures are available by virtue of severe plastic deformation(SPD)based techniques while the synthesis of growth HNT structures is so far almost *** addition,some representative molecular dynamics(MD)studies on the NT and HNT FCC metals unveil that the nanoscale effects such as twin spacing,grain size and plastic anisotropy greatly alter the performance of NT and HNT *** HNT structures may initiate unique phenomena in comparison with the NT ***,based on the phenomena and mechanisms revealed by experimental and MD simulation observations,a series of theoretical models have been *** are effective to describe the mechanical behaviors of NT and HNT metals within the applicable *** far the development of manufacturing technologies of HNT structures,as well as the studies on the effects of HNT structures on the properties of metals are still in its *** exploration is required to promote the design of advanced materials.

关键词： deformation twin ductility

Al含量对FeNiCoCrVAl高熵合金组织和力学性能的影响

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矿冶工程 2024年第3期44卷 170-175页

作者：尹荣章立钢中南大学材料科学与工程学院湖南长沙410083

通过XRD、SEM、EBSD和单轴拉伸测试,对不同Al含量(原子分数,%)Fe_(46-x)Ni_(20)Co_(20)Cr_(10)V_(4)Al_(x)(x=5,8,12,15)高熵合金的相结构、显微组织和力学性能进行了系统研究。结果表明,随着Al含量增加,Fe_(46-x)Ni_(20)Co_(20)Cr_(10)V_(4)Al_(x)高熵合金相结构由FCC单相转变为FCC+BCC双相结构,且强度随之增加,而塑性降低。其中,Fe_(34)Ni_(20)Co_(20)Cr_(10)V_(4)Al_(12)高熵合金在900℃退火处理后,具有极好的强塑性匹配,其屈服强度、抗拉强度和断裂延伸率分别为593 MPa、1009 MPa和32.13%。Al含量5%时,铸态和退火态高熵合金均为单一FCC相;Al含量8%,12%和15%时,高熵合金为FCC+BCC双相结构,退火处理促进了BCC相的析出,随着退火温度升高,BCC相占比增加。

关键词：高熵合金 FeNiCoCrV Al含量显微组织力学性能强塑性匹配

Super square carbon nanotube network: a new promising water desalination membrane

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npj Computational Materials 2016年第1期2卷 173-179页

作者： ligang Sun Xiaoqiao he Jian Lu Department of Architecture and Civil Engineering City University of Hong KongKowloonHong KongChina Department of Mechanical and Biomedical Engineering City University of Hong KongKowloonHong KongChina Centre for Advanced Structural Materials City University of Hong KongShenzhen Research InstituteShenzhen Hi-Tech Industrial ParkShenzhenChina

Super square(SS)carbon nanotube(CNT)networks,acting as a new kind of nanoporous membrane,manifest excellent water desalination *** in SS CNT network can efficiently filter NaCl from *** water desalination ability of such nanoporous membranes critically depends on the pore diameter,permitting water molecule permeatration while salt ion *** the basis of the systematical analysis on the interaction among water permeability,salt concentration limit and pressure on the membranes,an empirical formula is developed to describe the relationship between pressure and concentration *** the meantime,the nonlinear relationship between pressure and water permeability is ***,by controlling pressure,optimal plan can be easily made to efficiently filter the ***,steered molecular dynamics(MD)method uncovers bending and local buckling of SS CNT network that leads to salt ions passing through *** important mechanical behaviours are neglected in most MD simulations,which may overestimate the filtration ***,water permeability of such material is several orders of magnitude higher than the conventional reverse osmosis membranes and several times higher than nanoporous graphene *** CNT networks may act as a new kind of membrane developed for water desalination with excellent filtration ability.

关键词： network carbon networks