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Developmental toxicity of TCBPA on the nervous and cardiovascular systems of zebrafish (Danio rerio): A combination of transcriptomic and metabolomics

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Journal of Environmental Sciences 2023年第5期127卷 197-209页

作者： Wentao Liu Yifan Pan Lu Yang Yun Xie Xuanyue Chen Jing Chang Weiyu Hao lifei zhu Bin Wan Research Center for Eco-Environmental Sciences Chinese Academy of SciencesBeijing 100085China University of Chinese Academy of Sciences Beijing 100049China Agricultural Information Institute Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Food Safety Chinese Academy of Inspection and QuarantineBeijing 100176China Beijing Academy of Agriculture and Forestry Sciences Beijing 100097China

Tetrachlorobisphenol A(TCBPA),a widely used halogenated flame retardant,is frequently detected in environmental compartments and human samples.However,unknown developmental toxicity and mechanisms limit the entire understanding of its effects.In this study,zebrafish(Danio rerio)embryos were exposed to various concentrations of TCBPA while a combination of transcriptomics,behavioral and biochemical analyzes as well as metabolomics were applied to decipher its toxic effects and the potential mechanisms.We found that TCBPA could interfere with nervous and cardiovascular development through focal adhesion and extracellular matrix-receptor(ECM-receptor)interaction pathways through transcriptomic analysis.Behavioral and biochemical analysis results indicated abnormal swimming behavior of zebrafish larvae.Morphological observations revealed that TCBPA could cause the loss of head blood vessels.Metabolomic analysis showed that arginine-related metabolic pathways were one of the main pathways leading to TCBPA developmental toxicity.Our study demonstrated that by using omics,TCBPA was shown to have neurological and cardiovascular developmental toxicity and the underlying mechanisms were uncovered and major pathways identified.

关键词： Tetrachlorobisphenol A(TCBPA) Zebrafish Developmental toxicity Transcriptomics Metabolomics

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Self-consistent and precise measurement of time-dependent radiative albedo of gold based on specially symmetrical triple-cavity Hohlraum

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Matter and Radiation at Extremes 2024年第3期9卷 57-64页

作者： Zhiyu Zhang Yang Zhao Xiaoying Han Liling Li Bo Qing lifei Hou Yulong Li YuXue Zhang Huan Zhang Xiangming Liu Bo Deng Gang Xiong Min Lv Tuo zhu Chengwu Huang Tianming Song Yan Zhao Yingjie Li Lu Zhang Xufei Xie Jiyan Zhang Jiamin Yang Laser Fusion Research Center China Academy of Engineering PhysicsMianyang 621900China National Key Laboratory of Plasma Physcis Mianyang 621900China Institute of Applied Physics and Computational Mathematics Beijing 100088China

A self-consistent and precise method to determine the time-dependent radiative albedo,i.e.,the ratio of the reemission flux to the incident flux,for an indirect-drive inertial confinement fusion Hohlraum wall material is proposed.A specially designed symmetrical triple-cavity gold Hohlraum is used to create approximately constant and near-equilibrium uniform radiation with a peak temperature of 160 eV.The incident flux at the secondary cavity waist is obtained from flux balance analysis and from the shock velocity of a standard sample.The results agree well owing to the symmetrical radiation in the secondary cavity.A self-consistent and precise time-dependent radiative albedo is deduced from the reliable reemission flux and the incident flux,and the result from the shock velocity is found to have a smaller uncertainty than that from the multi-angle flux balance analysis,and also to agree well with the result of a simulation using the HYADES opacity.

关键词： precise cavity radiative

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Evaluation of SRAP markers efficiency in genetic diversity of Aspergillus flavus from peanut-cropped soils in China

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Oil Crop Science 2022年第3期7卷 135-141页

作者： Chushu Zhang lifei zhu Mian Wang Yueyi Tang Haixiang Zhou Qi Sun Qiang Yu Jiancheng Zhang Shandong Peanut Research Institute Key Laboratory of Peanut Biology and BreedingMinistry of Agriculture and Rural AffairsQingdao266100PR China Chongqing Normal University Chongqing400700PR China Qingdao Tianxiang Food Group Co. LtdQingdao266737PR China

In order to evaluation the efficiency of SRAP markers on genetic diversity of Aspergillus flavus,we screened out 17sets of primer pairs which could produce clear and reproducible SRAP bands from 150 SRAP primer pairs.The size of SRAP fragments ranged from 120 to 2100 bp.Primer pair Me10/Em9 produced the maximum number of polymorphic bands(12 bands),while Me8/Em13 produced the fewest number of polymorphic bands(only 1).Through analysis genetic diversity ability of different sets of primer pairs,the set of 12 primer pairs was selected for SRAP genetic marker of A.flavus.Cluster analysis was performed based on the genetic similarity coefficients,which ranged from 0.53 to 0.89.A dendrogram assembled using the unweighted pair-group method with arithmetic averages grouped A.flavus samples into 5 main clusters.The results suggested that SRAP marker is a useful molecular technology for the diversity of A.flavus from peanut soils in China.

关键词： Sequence-related amplified polymorphism Genetic diversity Aspergillus flavus Peanut Primer screening

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Inhalable oridonin-loaded poly(lactic-co-glycolic)acid large porous microparticles for in situ treatment of primary non-small cell lung cancer

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Acta Pharmaceutica Sinica B 2017年第1期7卷 80-90页

作者： lifei zhu Miao Li Xiaoyan Liu Lina Du Yiguang Jin Department of Pharmaceutical Sciences Beijing Institute of Radiation Medicine Anhui Medical University Department of Pharmacy Yangpu HospitalTongji University School of Medicine

Non-small cell lung cancer(NSCLC) accounts for about 85% of all lung cancers. Traditional chemotherapy for this disease leads to serious side effects. Here we prepared an inhalable oridonin-loaded poly(lactic-co-glycolic)acid(PLGA) large porous microparticle(LPMP) for in situ treatment of NSCLC with the emulsion/solvent evaporation/freeze-drying method. The LPMPs were smooth spheres with many internal pores. Despite a geometric diameter of 10 mm, the aerodynamic diameter of the spheres was only 2.72 mm, leading to highly efficient lung deposition. In vitro studies showed that most of oridonin was released after 1 h, whereas the alveolar macrophage uptake of LPMPs occurred after 8 h, so that most of oridonin would enter the surroundings without undergoing phagocytosis. Rat primary NSCLC models were built and administered with saline, oridonin powder, gemcitabine, and oridonin-loaded LPMPs via airway, respectively. The LPMPs showed strong anticancer effects. Oridonin showed strong angiogenesis inhibition and apoptosis. Relevant mechanisms are thought to include oridonin-induced mitochondrial dysfunction accompanied by low mitochondrial membrane potentials, downregulation of BCL-2 expressions, upregulation of expressions of BAX, caspase-3 and caspase-9. The oridonin-loaded PLGALPMPs showed high anti-NSCLC effects after pulmonary delivery. In conclusion, LPMPs are promising dry powder inhalations for in situ treatment of lung cancer.

关键词： Large porous microparticle Non-small cell lung cancer Oridonin Poly(lactic-co-glycolic) acid Pulmonary delivery

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Fungal community structure analysis of peanut pod rot in soil in Hebei Province,China

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Oil Crop Science 2023年第2期8卷 97-103页

作者： lifei zhu Chushu Zhang Yueyi Tang Haixiang Zhou Wenting Wang Mian Wang Qiang Yu Furong Song Jiancheng Zhang Shandong Peanut Research Institute Key Laboratory of Peanut Biology and Breeding Qingdao266100China Liaoning Technical University Fuxin123000China Qingdao Tianxiang Food Group Co.Ltd Qingdao266737China

In recent years,peanut yield and quality are more seriously affected by pod rot disease in China.However,managing this disease has proven challenging due to the wide host range of its pathogens.In this study,four soil samples were collected from fields with pod rot disease in Hebei Province,and 454 pyrosequencing was used to analyze the fungal communities structure within them.All 38490 ITS high-quality sequences were grouped into 1203 operational taxonomic units,the fungal community diversity of four soil samples was evaluated and compared using Shannon index and Simpson index.The results showed that members of Ascomycota were dominant,followed by Basidiomycota.According to the BLAST results at the species level,Guehomyces had the highest abundance,accounting for about 7.27%,followed by Alternaria,Fusarium,and Davidiella.The relative abundance of Fusarium oxysporum isolated from rotting peanuts in soil with peanut rot was higher than that in the control,indicating that Fusarium oxysporum might be one of the main pathogenic fungus of peanut rot in this area.This study delved into the broader fungal community associated with peanut pod rot,providing a theoretical foundation for preventing and treating this disease in agriculture.

关键词： Peanut pod rot Soil pathogens Fungal community Fusarium oxysporum

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面向高速铁路货运站选址的深度学习模型研究

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铁道学报 2024年第6期46卷 36-45页

作者：郑倩甘蜜姚竹魏力飞西南交通大学交通运输与物流学院四川成都610031 综合交通大数据应用技术国家工程实验室四川成都611756 综合交通运输智能化国家地方联合工程实验室四川成都611756

近年来急剧增长的快递量催生了高速铁路快运模式,高速铁路建设的持续推进为高速铁路快运体系的完善创造了新的发展契机。在规划过程中,高速铁路货运站的选址与特征之间存在复杂的异质性和非线性关系,因此本研究开发了一种基于深度学习... 详细信息

近年来急剧增长的快递量催生了高速铁路快运模式,高速铁路建设的持续推进为高速铁路快运体系的完善创造了新的发展契机。在规划过程中,高速铁路货运站的选址与特征之间存在复杂的异质性和非线性关系,因此本研究开发了一种基于深度学习的高速铁路货运站选址方法,该方法旨在根据现有的高速铁路快运物流基地建设项目挖掘出高速铁路货运站潜在的选址规律。研究初步构造市场需求、区位交通、发展环境3个维度的选址条件及其下属的10个指标,基于基础特征与深度学习算法构建选址模型,利用遗传算法优化超参数,同时根据各特征的重要度依次对模型进行测试以此确定出最佳的特征体系和模型学习层。实验结果表明,车站节点覆盖率、动车所预留情况、城市快递业务量、公路交通可达性和周边机场数目构成的特征子集在以LSTM和Dense堆叠的深度学习模型中表现出了最佳的选址效果,其F1分数达到0.9444,相比优化前有了较大幅度的提高。进一步以浙江省为例进行实证分析,选址结果也证实了研究所建立的深模型较好地学习了实际高速铁路货运站选址规律,可以帮助更加科学化、结构化的高速铁路货运站选址决策。

关键词：高速铁路货运站多源数据深度学习超参数优化长短期记忆网络

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Metabolic engineering of Escherichia coli for efficient ectoine production

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Systems Microbiology and Biomanufacturing 2021年第4期1卷 444-458页

作者： Shuyan Zhang Yu Fang lifei zhu Hedan Li Zhen Wang Ying Li Xiaoyuan Wang State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxi 214122China Key Laboratory of Industrial Biotechnology Ministry of EducationJiangnan UniversityWuxi 214122China International Joint Laboratory on Food Safety Jiangnan UniversityWuxi 214122China

Ectoine is a high-value stabilizer and protective agent with various applications in enzyme industry,cosmetics,and biomedi-cine.In this study,rational engineering strategies have been implemented in Escherichia coli to efficiently produce ectoine.First,the synthetic pathway of ectoine was constructed in E.coli MG1655 by introducing an artificial thermal switch system harboring the ectABC cluster from Halomonas elongate,and the resulting strain produced 1.95 g/L ectoine.Second,crr encoding the glucose-specific enzyme II domain A of phosphotransferase system and iclR encoding the glyoxylate shunt transcriptional repressor were deleted in E.coli for enhancing the oxaloacetate supply,leading to the increasement of the ectoine titer to 9.09 g/L.Third,thrA encoding the bifunctional aspartokinase/homoserine dehydrogenase was removed from the genome to weaken the competitive pathway;simultaneously,an endogenous feedback-resistant lysC was overexpressed to complement the enzymatic activity deficiency of the aspartate kinase,leading to 30.36%increase of ectoine titer.Next,the expression of phosphoenolpyruvate carboxylase was modulated with varying gradient strength promoters to accelerate the biosynthesis efficiency of ectoine.Finally,aspDH encoding aspartate dehydrogenase from Pseudomonas aeruginosa PAO1 was overexpressed to further improve the biosynthesis of ectoine.The final strain MWZ003/pFT28-ectABC-EclysC^(*)-aspDH-ppc3 produced 30.37 g/L ectoine after 36-h fed-batch fermentation with a yield of 0.132 g/g glucose and a productivity of 0.844 g/(L h).

关键词： Escherichia coli Rational engineering Ectoine production Precursor accumulation PaeAspDH Temperature-controlled fermentation

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The Brassicaceae-specific secreted peptides,STMPs,function in plant growth and pathogen defense

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Journal of Integrative Plant Biology 2020年第4期62卷 403-420页

作者： Zipeng Yu Yang Xu lifei zhu Lei Zhang Lin Liu Di Zhang Dandan Li Changai Wu Jinguang Huang Guodong Yang Kang Yan Shizhong Zhang Chengchao Zheng State Key Laboratory of Crop Biology Shandong Agricultural UniversityTai’an 271018China Shandong Peanut Research Institute Shandong Academy of Agricultural SciencesQingdao 266100China

Low molecular weight secreted peptides have recently been shown to affect multiple aspects of plant growth,development,and defense responses.Here,we performed stepwise BLAST filtering to identify unannotated peptides from the Arabidopsis thaliana protein database and uncovered a novel secreted peptide family,secreted transmembrane peptides(STMPs).These low molecular weight peptides,which consist of an N-terminal signal peptide and a transmembrane domain,were primarily localized to extracellular compartments but were also detected in the endomembrane system of the secretory pathway,including the endoplasmic reticulum and Golgi.Comprehensive bioinformatics analysis identified 10 STMP family members that are specific to the Brassicaceae family.Brassicaceae plants showed dramatically inhibited root growth uponexposure to chemically synthesized STMP1 and STMP2.Arabidopsis overexpressing STMP1,2,4,6,or 10 exhibited severely arrested growth,suggesting that STMPs are involved in regulating plant growth and development.In addition,in vitro bioassays demonstrated that STMP1,STMP2,and STMP10 have antibacterial effects against Pseudomonas syringae pv.tomato DC3000,Ralstonia solanacearum,Bacillus subtilis,and Agrobacterium tumefaciens,demonstrating that STMPs are antimicrobial peptides.These findings suggest that STMP family members play important roles in various developmental events and pathogen defense responses in Brassicaceae plants.

关键词： plant STM Arabidopsis

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3个美国油梨品种在广东湛江引种表现评价

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中国南方果树 2024年第1期53卷 83-90页

作者：朱利飞刘丽琴郑雪文胡小文杨转英广东海洋大学滨海农业学院广东湛江524000 中国热带农业科学院南亚热带作物研究所/国家热带果树种质资源圃/农业农村部热带果树生物学重点实验室广东湛江524000

为了解引入国家热带果树种质资源圃(广东省湛江市)的Hass、Tonnage和Wilson 3个美国油梨品种的生育期、果实品质性状和适应性等情况,对3个品种花形态、开花特性、开花授粉受精生理特征和主要果实性状等进行观测和比较分析。结果表明,3... 详细信息

为了解引入国家热带果树种质资源圃(广东省湛江市)的Hass、Tonnage和Wilson 3个美国油梨品种的生育期、果实品质性状和适应性等情况,对3个品种花形态、开花特性、开花授粉受精生理特征和主要果实性状等进行观测和比较分析。结果表明,3个油梨品种在广东省湛江市均能正常开花结果,花期均为2月下旬至4月中旬,花期比原产地短10~20 d,Hass和Tonnage为A型花,Wilson为B型花;Hass、Tonnage和Wilson花粉萌发率分别为87%、75%和82%,开花后3 h为最佳授粉期。Hass果实生理成熟期11月初,Tonnage为8月中旬,Wilson为9月初;果实风味、口感、颜色等性状与引种地基本一致,Wilson平均单果质量达到引种地的2倍多,而Tonnage单果质量只达到引种地的69.5%,Hass单果质量与原产地相近。

关键词：油梨 Hass 引种花期花粉萌发率单果质量

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生物柴油-柴油-DEC混合燃料发动机的喷雾、燃烧与排放性能分析

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内燃机与动力装置 2024年第1期41卷 8-16,29页

作者：赵礼飞祝先标王云鹏安徽江淮汽车集团股份有限公司安徽合肥230601

应用纹影法观察高压共轨系统下燃料的宏观喷雾特性,在高压共轨柴油机上开展外特性下燃烧和排放试验,分析生物柴油-柴油-碳酸二乙酯(diethyl carbonate,DEC)混合燃料的喷雾、燃烧与排放特性,生物柴油和柴油按体积比为3:7混合后的燃料记为... 详细信息

应用纹影法观察高压共轨系统下燃料的宏观喷雾特性,在高压共轨柴油机上开展外特性下燃烧和排放试验,分析生物柴油-柴油-碳酸二乙酯(diethyl carbonate,DEC)混合燃料的喷雾、燃烧与排放特性,生物柴油和柴油按体积比为3:7混合后的燃料记为B30,DEC和B30按体积比2:8掺混后的燃料记为B30DEC20。试验结果表明:混合性燃料B30DEC20的雾化效果明显提升,喷雾形态匀称、饱满,喷雾锥角、喷雾浓度等接近柴油;外特性运行时,燃用B30DEC20的发动机转矩比燃用柴油低27.4 N·m,但其热效率比燃用柴油高,转速为2400 r/min时,燃用B30DEC20的发动机热效率比燃用柴油高1.5%;B30DEC20的含氧属性使其具有较低的颗粒物(particulate matter,PM)和CO排放,燃用该燃料的发动机PM排放比燃用柴油降低65%~85%,THC排放低于燃用柴油;虽然燃用B30DEC20发动机的NO_(x)比排放大于燃用其他燃料,但当发动机转速大于2400 r/min后,燃用B30DEC20发动机的NO_(x)排放低于燃用柴油。

关键词：生物柴油柴油 DEC 喷雾燃烧排放

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