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Improvement in bioconversion efficiency and reduction of ammonia emission by introduction of fruit fermentation broth in a black soldier fly larvae and kitchen waste conversion system

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Insect Science 2023年第4期30卷 975-990页

作者： Lusheng Li lifei chen Rongsheng Shang Guiying Wang Jibin Zhang School of Agricultural Science and Engineering Engineering Research Center of Shandong Province for Black Soldier Fly Breeding and Organic Waste ConversionLiaocheng UniversityLiaochengShandong ProvinceChina State Key Laboratory of Agricultural Microbiology National Engineering Research Centre of Microbial PesticidesCollege of Life Science and TechnologyCollege of Animal Science&TechnologyHuazhong Agricultural UniversityWuhanChina

The black soldier fly(BSF),Hermetia illucens(Diptera:Stratiomyidae),is an insect commonly used for the bioconversion of various organic wastes.Not only can the BSF convert organic waste into macromolecular organic substances,such as insect pro-teins,but it can also lessen the pollution associated with these waste products by reducing ammonia emissions,for example.In this study,we measured the effects of adding fruit fermentation broth(Fer)and commercial lactic acid bacteria fermentation broth(Em)to kitchen waste(KW),as deodorizing auxiliary substances,on the growth performance of black soldier fly larvae(BSFL),the intestinal flora structure of BSFL,the ammonia emis-sion from the KW substrate,and the microbial community structure of the KW substrate.We found that the addition of Fer or Em increased the body weight of BSFL after 6 d of culture,increasing the growth rate by 9.96%and 7.96%,respectively.The addition of Fer not only reduced the pH of the KW substrate but also increased the relative abundance of probiotics,such as Lactobacillus,Lysinibacillus,and Vagococcus,which inhibited the growth of ammonifiers such as Bacillus,Oligella,Paenalcaligenes,Paenibacillus,Pseu-dogracilibacillus,and Pseudomonas,resulting in the reduction of ammonia emission in the KW substrate.Moreover,the addition of Fer or Em significantly increased the rela-tive abundances of Bacteroides,Campylobacter,Dysgonomonas,Enterococcus,and Ig-natzschineria in the gut of BSFL and increased the species diversity and richness in the K W substrate.Our findings provide a novel way to improve the conversion rate of organic waste and reduce the environmental pollution caused by BSF.

关键词： ammonia emissions black soldier fly fruit fermentation broth growth per-formance kitchen waste lactic acid bacteria fermentation broth

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Metabolomics provide insights into the endogenous mechanism of strobilation in the scyphozoan jellyfi sh Rhopilema esculentum

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Journal of Oceanology and Limnology 2022年第1期40卷 226-234页

作者： Jianlong GE Xiaohui chen Changlin LIU Jie TAN Li BIAN lifei chen Siqing chen Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesLaboratory for Marine Fisheries Science and Food Production ProcessesPilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China Yancheng Huayi Aquatic Products Co.Ltd. Yancheng 224300China

Strobilation is a well-organized metamorphosis process in scyphozoan jellyfi sh,through which the benthic polyp develops into the pelagic medusa.So far,knowledge on the molecular mechanisms behind the drastic morphological and physiological changes remains limited.In the present study,the metabolic profi les in polyp and strobila stages of jellyfi sh Rhopilema esculentum were examined using ultra-performance liquid chromatography coupled to a mass spectrometer.A total of 3071 metabolites with putative annotation were detected,of which 167 were identifi ed as diff erential metabolites between the polyp and strobila.Among the metabolites,31 signifi cantly decreased and 136 signifi cantly increased in abundance in the strobila.Thyroxin,one of the previously proposed strobilation inducer of Aurelia,was not detected in this study.The indole-containing compounds are known for triggering strobilation in Discomedusae.Two indole derivatives and an indole-isomer containing metabolite were detected among the most signifi cantly increased metabolites,which may be potential endogenous molecules for strobilation initiation.The membrane-associated phospholipids and the infl ammation and oxidative stress-related eicosanoids were found to have signifi cant changes.Although the functions of these metabolites in strobilation are not yet completely known,they provide some clues to the induction of the process of strobilation and metabolic responses that take place during strobilation.

关键词： Rhopilema esculentum scyphozoan jellyfi sh strobilation metabolomics

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3D printed lactic acid bacteria hydrogel:cell release kinetics and stability

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Food Science and Human Wellness 2023年第2期12卷 477-487页

作者： Yifei Liu Xintao Yin Xiudong Xia Zhen Liu lifei chen Mingsheng Dong College of Food Science and Technology Nanjing Agricultural UniversityNanjing 210095China Institute of Agricultural Product Processing Jiangsu Academy of Agricultural SciencesNanjing 210014China

In this study,a new type of 3 D printed living biological hydrogel was developed by integrating lactic acid bacteria(LAB)into biocompatible and non-toxic polymer materials.Interestingly,the living materials loaded with LAB can be freeze-dried and reused for more than 100 times.The bio-hydrogel can be used to co-culture different LAB and keep its fermentation performance stable in long-term use.The release kinetics model and response surface method were used to simulate and optimize the bacteria release mode in the bio-hydrogel.The results show that the release of bacteria from hydrogel is regulated by the coupling of Fickian diffusion and polymer swelling.The stability of LAB hydrogel was evaluated by reuse experiments.The images of confocal microscopy and scanning electron microscope showed that the bacteria with high cell viability were distributed in the hydrogel and intact structure of the living hydrogel was maintained after 100 times of reuse as yoghurt starter.In conclusion,the 3 D printed LAB bio-hydrogel developed in this study has the advantage of reuse and sustainability,which is expected to open up a new way for the preparation of food culture starter.

关键词： 3D printing Bioink Living hydrogel Lactic acid bacteria Culture starter

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Center-based clustering of categorical data using kernel smoothing methods

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Frontiers of Computer Science 2018年第5期12卷 1032-1034页

作者： Xuanhui YAN lifei chen Gongde GUO School of Mathematics and Informatics Fujian Normal University Fuzhou 350007 China

1 Introduction Currently, categorical data are world applications, particularly widely used in many realin the biomedical and social sciences. Unlike the numeric case, when the data are cate- gorical, choosing the appropriate similarity or dissimilarity measure between two different categorical objects became a difficult task for many applications. What＇s more, in this case, the set mean, i.e., the averaging value of a finite set of data objects, is an undefined concept. This means that the well known K-means [ 1 ] and its numerous variants cannot be directly used for center-based clustering of categorical data.

关键词： ALGORITHM

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物联网技术支持下的城市热环境时空变化分析

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生态学报 2024年第7期44卷 2849-2858页

作者：郭冠华陈丽飞曹峥吴志峰陈颖彪广州大学地理科学与遥感学院广州511006

热环境是城市生态系统最为关键的大气环境要素之一,开展城市热环境研究的前提是在时空维度上获取足量的热环境参数。利用先进的物联网技术构建了在线式热环境监测设备,收集2022年10月16日至24日广州大学校园气温、风速、太阳辐射和地面... 详细信息

热环境是城市生态系统最为关键的大气环境要素之一,开展城市热环境研究的前提是在时空维度上获取足量的热环境参数。利用先进的物联网技术构建了在线式热环境监测设备,收集2022年10月16日至24日广州大学校园气温、风速、太阳辐射和地面温度四种热环境参数,分析小尺度下城市热环境的时空变化特征。研究结果表明:1)不同测点之间的风速特征具有一定的相关性,极个别测点的风速特征与其它测点相关性仅在0.5左右,显示其小气候的独特性;2)不同测点风速和热岛强度变化时空差异明显,即使距离靠近的测点,受邻近建筑和植被特征的影响热环境特征会有所差异,各测点的日间风速大,热岛强度较为明显,夜间风速较小,热岛强度较弱;3)地面温度与气温的相关性达0.8左右,这种相关性在夜间表现更为密切,并且这种关系受风速的影响不大。研究结果反映了城市热环境参数在小尺度上的高度异质性,并揭示了物联网技术在城市热环境监测领域的可行性、便捷性和高效性。

关键词：城市热环境时空变化物联网广州校园

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Elevated atmospheric CO_(2) impact on carbon and nitrogen transformations and microbial community in replicated wetland

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Ecological Processes 2020年第1期9卷 677-688页

作者： Dawei Jiang lifei chen Nan Xia Eyram Norgbey Desmond Ato Koomson Williams Kweku Darkwah College of Horticulture Jilin Agricultural UniversityChangchun 130118China Jilin Tiandiren Landscape Architecture Design Engineering Co.Ltd. Changchun 130117China Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes Ministry of EducationEnvironmental Engi neering DepartmentCollege of EnvironmentHohai University1 Xikang RoadGulou210098 NanjingChina

Background:Elevated atmospheric CO_(2) has direct and indirect influences on ecosystem processes.The impact of elevated atmospheric CO_(2) concentration on carbon and nitrogen transformations,together with the microbial community,was evaluated with water hyacinth(Eichhornia crassipes)in an open-top chamber replicated wetland.The responses of nitrogen and carbon pools in water and wetland soil,and microbial community abundance were studied under ambient CO_(2) and elevated CO_(2)(ambient+200μL L^(−1)).Results:Total biomass for the whole plant under elevated CO_(2) increased by an average of 8%(p=0.022).Wetlands,with water hyacinth,showed a significant increase in total carbon and total organic carbon in water by 7%(p=0.001)and 21%(p=0.001),respectively,under elevated CO_(2) compared to that of ambient CO_(2).Increase in dissolved carbon in water correlates with the presence of wetland plants since the water hyacinth can directly exchange CO_(2) from the atmosphere to water by the upper epidermis of leaves.Also,the enrichment CO_(2) showed an increase in total carbon and total organic carbon concentration in wetland soil by 3%(p=0.344)and 6%(p=0.008),respectively.The total nitrogen content in water increased by 26%(p=0.0001),while total nitrogen in wetland soil pool under CO_(2) enrichment decreased by 9%(p=0.011)due to increased soil microbial community abundance,extracted by phospholipid fatty acids,which was 25%larger in amount than that of the ambient treatment.Conclusion:The study revealed that the elevated CO_(2) would affect the carbon and nitrogen transformations in wetland plant,water,and soil pool and increase soil microbial community abundance.

关键词： Elevated CO_(2) Carbon transformation Nitrogen transformation Microbial community abundance Phospholipid fatty acids Open-top chamber

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科技创新与乡村建设的时空耦合及驱动因素研究--以宁夏回族自治区为例

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天津商业大学学报 2024年第3期44卷 36-43页

作者：李苏邵明珠郭远通陈莉菲北方民族大学经济学院银川750021 国家民委人文社会科学重点研究基地民族地区相对贫困治理研究中心银川750021

科技乡村融合发展是实现农业农村现代化的必然要求。本文采用2013—2021年宁夏13个县(市)的面板数据,运用耦合协调模型分析科技创新与乡村建设的耦合协调发展时空格局,继而构建空间杜宾模型探究二者耦合协调的空间驱动机制。研究表明:... 详细信息

科技乡村融合发展是实现农业农村现代化的必然要求。本文采用2013—2021年宁夏13个县(市)的面板数据,运用耦合协调模型分析科技创新与乡村建设的耦合协调发展时空格局,继而构建空间杜宾模型探究二者耦合协调的空间驱动机制。研究表明:观测期内二者的耦合协调度总体呈上升状态;在空间上,二者的耦合协调度呈现出显著的“北高南低”层级递减特征。各县(市)耦合协调具有显著的空间集聚特征,主要表现为“高—高”和“低—低”两种集聚模式。进一步研究发现,城乡消费均衡、研发经费支出、技术合同项数等因素对耦合协调呈显著正向影响。因此,在宁夏科技乡村融合过程中需要明晰地区间各因素的发展状况,完善科技乡村耦合机制,着力解决好区域间发展不平衡的问题。

关键词：科技创新乡村建设耦合协调模型空间杜宾模型

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A probabilistic framework for optimizing projected clusters with categorical attributes

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Science China(Information Sciences) 2015年第7期58卷 144-158页

作者： chen lifei School of Mathematics and Computer Science Fujian Normal University

The ability to discover projected clusters in high-dimensional data is essential for many machine learning applications. Projective clustering of categorical data is currently a challenge due to the difficulties in learning adaptive weights for categorical attributes coordinating with clusters optimization. In this paper,a probability-based learning framework is proposed, which allows both the attribute weights and the centerbased clusters to be optimized by kernel density estimation on categorical attributes. A novel algorithm is then derived for projective clustering on categorical data, based on the new learning approach for the kernel bandwidth selection problem. We show that the attribute weight substantially connects to the kernel bandwidth, while the optimized cluster center corresponds to the normalized frequency estimator of the categorical attributes.Experimental results on synthesis and real-world data show outstanding performance of the proposed method,which significantly outperforms state-of-the-art algorithms.

关键词： projective clustering projected cluster categorical data probabilistic framework kernel density estimation attribute weighting

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表面负载金属纳米粒子的氮化物陶瓷导热填料的制备与表征

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上海第二工业大学学报 2024年第1期41卷 9-18页

作者：陈燕杰董华蕊马清怡赵雪伶陈立飞上海第二工业大学能源与材料学院上海201209 上海第二工业大学资源与环境工程学院上海201209 上海第二工业大学上海先进热功能材料工程技术研究中心上海201209

采用直接混合加热法制备氮化物陶瓷复合导热填料,这是一种可以批量生产,并且不使用溶剂、对环境友好的制备方法。通过扫描电镜、X射线衍射仪、TCI导热仪研究不同金属纳米粒子(镍、钴和银)在氮化物(氮化铝、氮化硼)上的负载情况以及负载... 详细信息

采用直接混合加热法制备氮化物陶瓷复合导热填料,这是一种可以批量生产,并且不使用溶剂、对环境友好的制备方法。通过扫描电镜、X射线衍射仪、TCI导热仪研究不同金属纳米粒子(镍、钴和银)在氮化物(氮化铝、氮化硼)上的负载情况以及负载量,并考察了复合陶瓷导热填料对聚乙烯醇基复合导热膜导热性能的影响。结果表明,金属纳米粒子成功负载到氮化物上而不改变氮化物的晶体结构。且负载金属粒子的尺寸随乙酸盐含量的增加而增大。另外,负载金属粒子的陶瓷导热填料能有效提高聚乙烯醇基复合导热膜导热能力,Ag/AlN-0.05导热膜的导热率能达到0.752 W/(m·K)。

关键词：氮化铝氮化硼乙酸盐金属粒子导热填料

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全身麻醉对婴幼儿大脑发育影响的临床研究

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右江医学 2024年第4期52卷 313-318页

作者：杨影陈丽斐覃银莹李春来谢玉波广西医科大学第一附属医院麻醉科广西南宁530021 广西消化道肿瘤加速康复外科基础研究重点实验室广西南宁530021

目的探讨全身麻醉对婴幼儿术后大脑发育的影响。方法对2012—2015年期间年龄为0~3岁时在广西医科大学第一附属医院接受全身麻醉下的手术治疗儿童(研究组)共429例在其3~6岁时进行回顾性分析。按照研究组儿童生活的城市地区、年龄、性别... 详细信息

目的探讨全身麻醉对婴幼儿术后大脑发育的影响。方法对2012—2015年期间年龄为0~3岁时在广西医科大学第一附属医院接受全身麻醉下的手术治疗儿童(研究组)共429例在其3~6岁时进行回顾性分析。按照研究组儿童生活的城市地区、年龄、性别、民族等特征采取1∶1配对设计,选取0~3岁期间未接受过全身麻醉的3~6岁儿童共429例作为对照。查阅和记录研究组儿童的麻醉记录单及基本信息,将研究组儿童按麻醉时长分为三组:2 h组(A3组),按手术麻醉次数分为两组:单次组(B1组)、2次组(B2组),按苏醒时间分为三组:60 min组(C3组)。采用韦氏幼儿智力量表第四版(WPPSI-IV)综合评估上述儿童的总体认知能力及智力活动水平,Achenbach儿童行为量表(CBCL)总得分评估儿童行为、情绪和社会能力,盖塞尔发展量表(Gesell)评价儿童的中枢神经系统功能及发育水平。结果适用2岁6个月~4岁前韦氏智力量表的儿童中:与对照组比较,研究组儿童的工作记忆指数得分降低;适用4岁~6岁11个月韦氏智力量表的儿童中:与对照组比较,研究组儿童的言语理解指数、流体推理指数、加工速度指数、工作记忆指数得分和盖塞尔量表的发育商数降低(P0.05)。结论婴幼儿在大脑发育期接受全身麻醉可能对其神经系统发育产生影响,接受2次或时长大于2 h全身麻醉的婴幼儿在智力发育的过程中的短时记忆和存储、加工以及输出信息的能力更易受影响。

关键词：全身麻醉婴幼儿大脑发育临床研究

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