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Brain circuits for retching-like behavior

National Science Review 2024年第1期11卷 145-160页

作者： lifang huo Zhimin Ye Meiling Liu Ziqing He Meizhu Huang Dapeng Li Qian Wu Qian Wang Xiaoqun Wang Peng Cao Ji Dong Congping Shang School of Basic Medical Sciences Guangzhou National Laboratory The Fifth Afflliated Hospital Guangzhou Medical University Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Department of Neurobiology School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and Repair Advanced Innovation Center for Human Brain Protection Capital Medical University State Key Laboratory of Cognitive Neuroscience and Learning IDG/McGovern Institute for Brain Research Beijing Normal University Changping Life Science Laboratory National Institute of Biological Sciences

Nausea and vomiting are important defensive responses to cope with pathogens and toxins that invade the body. The nucleus of the solitary tract(NTS) is important for initiating these responses. However, the molecular heterogeneities and cellular diversities of the NTS occlude a better understanding of these defensive responses. Here, we constructed the single-nucleus transcriptomic atlas of NTS cells and found multiple populations of NTS neurons that may be involved in these defensive responses. Among these, we identified Calbindin1-positive(Calb1+) NTS neurons that are molecularly distinct from Tac1+neurons.These Calb1+neurons are critical for nausea and retching induced by cereulide; an emetic toxin secreted by Bacillus Cereus. Strikingly, we found that cereulide can directly modulate vagal sensory neurons that innervate Calb1+NTS neurons, a novel mechanism distinct from that for nausea and retching induced by Staphylococcal enterotoxin A. Together, our transcriptomic atlas of NTS neurons and the functional analyses revealed the neural mechanism for cereulide-induced retching-like behavior. These results demonstrate the molecular and cellular complexities in the brain that underlie defensive responses to the diversities of pathogens and toxins.

关键词： vomiting nausea nucleus of the solitary tract Calbindin1 neural circuits

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Potentiation of PIEZO2 mechanically-activated currents in sensory neurons mediates vincristine-induced mechanical hypersensitivity

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Acta Pharmaceutica Sinica B 2023年第8期13卷 3365-3381页

作者： Mingli Duan Yurui Jia lifang huo Yiting Gao Jia Wang Wei Zhang Zhanfeng Jia Department of Pharmacology Hebei Medical UniversityShijiazhuang 050017China Center of Innovative Drug Research and Evaluation Institute of Medical Science and HealthHebei Medical UniversityShijiazhuang 050017China The Key Laboratory of Neural and Vascular Biology Ministry of EducationShijiazhuang 050017China The Key Laboratory of New Drug Pharmacology and Toxicology Shijiazhuang 050017China The Hebei Collaboration Innovation Center for Mechanism Diagnosis and Treatment of Neurological and Psychiatric DiseaseShijiazhuang 050017China Department of Pharmacology Institute of Chinese Integrative MedicineHebei Medical UniversityShijiazhuang 050017China

Vincristine,a widely used chemotherapeutic agent for treating different cancer,often induces severe peripheral neuropathic pain.A common symptom of vincristine-induced peripheral neuropathic pain is mechanical allodynia and hyperalgesia.However,mechanisms underlying vincristine-induced mechanical allodynia and hyperalgesia are not well understood.In the present study,we show with behavioral assessment in rats that vincristine induces mechanical allodynia and hyperalgesia in a PIEZO2 channel-dependent manner since gene knockdown or pharmacological inhibition of PIEZO2 channels alleviates vincristine-induced mechanical hypersensitivity.Electrophysiological results show that vincristine potentiates PIEZO2 rapidly adapting(RA)mechanically-activated(MA)currents in rat dorsal root ganglion(DRG)neurons.We have found that vincristine-induced potentiation of PIEZO2 MA currents is due to the enhancement of static plasma membrane tension(SPMT)of these cells following vincristine treatment.Reducing SPMT of DRG neurons by cytochalasin D(CD),a disruptor of the actin filament,abolishes vincristine-induced potentiation of PIEZO2 MA currents,and suppresses vincristine-induced mechanical hypersensitivity in rats.Collectively,enhancing SPMT and subsequently potentiating PIEZO2 MA currents in primary afferent neurons may be an underlying mechanism responsible for vincristineinduced mechanical allodynia and hyperalgesia in rats.Targeting to inhibit PIEZO2 channels may be an effective analgesic method to attenuate vincristine-induced mechanical hypersensitivity.

关键词： Vincristine Peripheral neuropathic pain Mechanical hypersensitivity Dorsal root ganglion neurons PIEZO2mechanically-activated currents

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油酸锌/脲嘧啶复合热稳定剂对PVC性能的影响

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塑料 2024年第1期53卷 42-47页

作者：宋莉芳李开元黄卓辰霍慧文夏慧芸牛艳辉长安大学材料科学与工程学院陕西西安710061

为了改善软质聚氯乙烯(PVC)的初始白度和长期热稳定性,制备由具有不饱和链的油酸锌和含氨基的脲嘧啶(6-氨基-1,3-二甲基脲嘧啶,DAU)组成的复合热稳定剂,添加合适的辅助稳定剂和增塑剂,进行聚氯乙烯热加工。采用刚果红试验法和干燥箱热... 详细信息

为了改善软质聚氯乙烯(PVC)的初始白度和长期热稳定性,制备由具有不饱和链的油酸锌和含氨基的脲嘧啶(6-氨基-1,3-二甲基脲嘧啶,DAU)组成的复合热稳定剂,添加合适的辅助稳定剂和增塑剂,进行聚氯乙烯热加工。采用刚果红试验法和干燥箱热老化试验法分析复合稳定剂对PVC静态热稳定性的影响;采用转矩流变测试评价其对PVC动态热稳定性的影响;利用红外光谱法研究复合稳定剂的热稳定机理。结果表明,油酸锌与脲嘧啶复合后,稳定剂与PVC的相容性提高,初始白度得到改善;复合稳定剂中DAU与油酸锌(DAU/Zn)的最佳比例为4∶1,长期热稳定性可达100 min;另外,加入辅助稳定剂和增塑剂使PVC的热稳定效果得到进一步改善。

关键词：聚氯乙烯油酸锌脲嘧啶热稳定性热稳定机理

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A 1G-cell floating-gate NOR flash memory in 65 nm technology with 100 ns random access time

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Science China(Information Sciences) 2015年第4期58卷 158-165页

作者： LIU lifang WU Dong LIU XueMei huo ZongLiang LIU Ming PAN LiYang Institute of Microelectronics University of Tsinghua Tsinghua National Laboratory for Information Science and Technology Laboratory of Nano-Fabrication and Novel Devices Integrated Technology Institute of MicroelectronicsChinese Academy of Sciences

A 1G-cell NOR flash memory chip has been designed and fabricated successfully with 65 nm technology. To compromise the array efficiency and chip speed, the paper establishes an array model including parasitics of the whole array, and optimizes the sector structure as 512 wordlines(WLs) and 4096 bitlines(BLs).Furthermore, by adding other models of long and thin metal lines, we have analyzed the speed of critical circuit nodes. As a result, the agreement of WL delay between simulation and measurement verifies the accuracy of the array model and lines models. The test results indicate that the chip achieves random access time of 100 ns and page read time of 25 ns under 3.3 V voltage supply.

关键词： NOR flash memory giga-level 65 nm technology array efficiency high speed

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Exploring the Mechanism of CircRNA-vgll3 in Osteogenically Differentiated Human Bone Marrow Mesenchymal Stem Cells

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Journal of Clinical and Nursing Research 2023年第4期7卷 151-158页

作者： Yajie huo Yu Mao Fang Luo Fengjiao Zhang lifang Xie Xiaoke Zhang Kai Liu Ling Sun Hongmei Liu Lige Song Huanhuan Wang Zhiqiang Kang Department of Endocrinology Zhengzhou Central Hospital Affiliated to Zhengzhou UniversityZhengzhou 450007Henan ProvinceChina

Objective:To explore the mechanism of circRNA-vgll3 in osteogenic differentiation of human bone marrow mesenchymal stem cells.Methods:BMSCs cells were transfected with circRNA-vgll3,and divided into circRNA-vgll3 high-level group,circRNA-vgll3 low-level group,and negative control group(circRNA-vgll3 not transfected)according to the amount of transfection.The proliferation and apoptosis of BMSCs osteoblasts in each group were analyzed,and the alkaline phosphatase(ALP)activity,type I collagen gray value,bone morphogenetic protein 2(BMP-2),Runx2 protein,and mRNA expression levels were detected.Results:The circRNA-vgll3 low-level group had a significant inhibitory effect on the proliferation of BMSCs osteoblasts,and the apoptosis rate of the circRNA-vgll3 low-level group was significantly higher than that of the circRNA-vgll3 high-level group(P<0.05);ALP activity,type I collagen gray value,BMP-2,Runx2 protein,and mRNA expression levels in the high-level circRNA-vgll3 group were significantly higher than those in the low-level circRNA-vgll3 group,and the difference was statistically significant(P<0.05).Conclusion:Overexpression of circRNA-vgll3 can promote the osteogenic differentiation ability of BMSCs,while low expression of circRNA-vgll3 can inhibit the osteogenic differentiation ability of BMSCs.The main mechanism of action is that circRNA-vgll3 can affect osteogenic differentiation by regulating the Runx2 protein.

关键词： CircRNA-vgll3 Osteogenic differentiation Human bone marrow mesenchymal stem cells Mechanism of action

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偶特征正交空间上非奇异子空间的Critical问题

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数学进展 2014年第6期43卷 824-834页

作者：霍丽芳赵燕冰霍元极河北建筑工程学院数理系张家口河北075000 张家口职业技术学院基础部张家口河北075051 海南软件职业技术学院基础部琼海海南571000

利用偶特征有限正交空间的性质及计数定理在偶特征有限正交空间上研究了非奇异子空间的critical问题,得到了相应的计数公式和critical指数.

关键词：偶特征有限正交空间 critical指数格 matroid Mobius函数

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软质PVC加工用油酸-辛酸钙/锌液体热稳定剂的制备与性能

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塑料工业 2022年第12期50卷 140-146,169页

作者：宋莉芳李开元霍慧文夏慧芸牛艳辉长安大学交通铺面材料教育部工程研究中心陕西西安710061 长安大学材料科学与工程学院陕西西安710061

以油酸、辛酸两种长链脂肪酸为原料,在变压器油中一步合成了油酸-辛酸混合酸钙/锌液体稳定剂,并将其用于聚氯乙烯(PVC)热加工过程。采用刚果红实验法和烘箱热老化法考察了液体稳定剂对PVC静态热稳定性的影响,并研究了辅助热稳定剂的协... 详细信息

以油酸、辛酸两种长链脂肪酸为原料,在变压器油中一步合成了油酸-辛酸混合酸钙/锌液体稳定剂,并将其用于聚氯乙烯(PVC)热加工过程。采用刚果红实验法和烘箱热老化法考察了液体稳定剂对PVC静态热稳定性的影响,并研究了辅助热稳定剂的协同稳定作用;通过转矩流变测试评价了其对PVC动态热稳定性的影响;通过扫描电镜观察了稳定剂在PVC中的分散性。结果表明,当液体稳定剂中钙锌比(物质的量比)为2.8∶1时,PVC的静态热稳定性最佳;保持液体稳定剂钙锌总物质的量一定,研究了单独添加有机酮、季戊四醇、三亚磷酸苯酯时PVC体系的静态热稳定性,结果表明,季戊四醇对刚果红变色时间的提高最明显,三亚磷酸苯酯对初始白度影响最大;复合稳定剂热降解时,由于产生HCl而使PVC表面留有均匀分布的微孔,表明复合稳定剂在PVC中分散较为均匀。

关键词：聚氯乙烯油酸辛酸液体稳定剂

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白鹤灵芝总萘醌对高脂饮食诱导的非酒精性脂肪肝小鼠的作用研究

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食品工业科技 2023年第1期44卷 389-397页

作者：龚志强夏雨弘韩沙黄艳红霍宇陈晓兰蒙田秀杨立芳广西民族大学化学化工学院广西林产化学与工程重点实验室广西多糖材料与改性重点实验室广西南宁530008 广西中医药大学赛恩斯新医药学院广西南宁530222

为评价白鹤灵芝总萘醌(Rhinacanthins-rich extract from Rhinacanthus nasutus,RRE)的降血脂及保护肝脏作用,采用高脂饲料诱导建立的非酒精性脂肪肝(Nonalcoholic Fatty Liver Disease,NAFLD)小鼠模型,通过测定小鼠血清中总胆固醇(Total Cholesterol,TC)、甘油三酯(Triglyceride,TG)、高密度脂蛋白(High Density Lipoprotein,HDL-C)、低密度脂蛋白(Low Density Lipoprotein,LDL-C)及肝脏及谷草转氨酶(Aspartate transaminase,AST)、谷丙转氨酶(Alanine transaminase,ALT)、碱性磷酸酶(Alkaline phosphatase,AKP)的水平,以及肝脏组织进行病理学观察。动物实验结果表明,RRE干预后有效改善了NAFLD小鼠肝脏病理病变进程,缓解炎性损伤,减少肝脏脂肪颗粒堆积;高、中剂量均能显著降低NAFLD小鼠血清中TC、TG、LDL-C水平(P0.05);通过网络药理学对白鹤灵芝与NAFLD疾病相关作用靶点进行交集,结合分子对接技术对RRE的有效活性物质及作用机制进行预测,结果表明白鹤灵芝素-C(rhinacanthin-C)与交集靶点具有良好的结合潜力。研究表明RRE有显著的降血脂、改善脂质沉积和炎性损伤、保护肝脏的作用,其作用机制可能是rhinacanthin-C经由AMPK-SREBP1c-SIRT1信号调节脂质代谢紊乱发挥作用,为壮药白鹤灵芝开发为降血脂、改善脂肪肝的民族药物提供实验依据。

关键词：白鹤灵芝总萘醌非酒精性脂肪肝降血脂保护肝脏

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^(18)F-FDG PET/MR Imaging for Evaluating of Obstructive Jaundice:Chinese Expert Consensus

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Clinical Cancer Bulletin 2022年第2期1卷 56-65页

作者： lifang PANG Yushen GU Zhi YANG Zhifang WU Wei FAN Xiaohua ZHU Jun ZHAO Changjiu ZHAO Xuemei WANG Yongju GAO Li huo Yi MO Meiyun WANG Jie LU Lingzhi HU Zheng ZHANG Feng WANG Xuemei DU Yue CHEN Jianjun LIU Zhaoping CHENG Yunlong LOU Shuzhan YAO Rong XU Sijin LI Hongcheng SHI Department of Nuclear Medicine Zhongshan HospitalFudan UniversityShanghai Institute of Medical ImagingInstitute of Nuclear MedicineFudan UniversityCancer Prevention and Treatment CenterZhongshan HospitalFudan UniversityNo.180 Fenglin RoadShanghai 200032China Department of Nuclear Medicine Peking University Cancer Hospital&InstituteNo.52 Fucheng RoadBeijing 100142China Department of Nuclear Medicine First Hospital of Shanxi Medical UniversityTaiyuan 030001China Department of Nuclear Medicine Sun Yat-sen University Cancer CenterNo.651 Dongfendong RoadGuangzhou 510060China Department of Nuclear Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and Technology1095 Jiefang AveWuhan 430030China Department of Nuclear Medicine Shanghai East HospitalSchool of MedicineTongji UniversityNo.150 Jimo RoadShanghai 200120China Department of Nuclear Medicine First Affiliated Hospital of Harbin Medical UniversityNo.23Youzheng StreetHarbin 150001China Department of Nuclear Medicine Inner Mongolia Medical University Affilated HospitalNo.1 Tongdao North StreetHohhot 010050China Department of Nuclear Medicine Henan Provincial People’s HospitalZhengzhou 450003China Department of Nuclear Medicine Peking Union medical college HospitalNo.1 ShuaifuyuanBeijing 100730China Department of PET-CT center Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of MedicineCentral South UniversityNo.283Tongzipo RoadChangsha 410013China Department of Medical Imaging Henan Provincial People's HospitalNo.7 Weiwu RoadZhengzhouHenan 450003China Department of Radiology and Nuclear Medicine Xuanwu Hospital Capital Medical UniversityNo.45 Changchun StreetBeijing 100053China Shanghai United Imaging Healthcare Co. Ltd.Shanghai 201807China Department of Nuclear Medicine Nanjing First HospitalNanjing Medical University68 Changle RoadNanjing 210006China Department of Nuclear Medicine the First Affiliated Hospital of Dalian Medical UniversityNo.222 Zhongshan Road

The cause of obstructive jaundice is usually complex which renders its differential diagnosis and lesion localization challenging in clinical practice.Integrated Positron Emission tomography/Magnetic Resonance(PET/MR)offers complementary information from PET and MR in the diagnosis of obstructive jaundice and is becoming widely adopted in clinical setting.While preserving its diagnostic accuracy,it is important to standardize and streamline the clinical scan protocol of PET/MR in evaluating obstructive jaundice.Based on literature review and experience of large number of clinical cases from the author group,this article reports an expert consensus on imaging protocol optimization and case interpretation template standardization.

关键词： hepatobiliary system obstructive jaundice positron emission tomography/magnetic resonance imaging(PET/MR) consensus

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酉空间上全迷向子空间的Critical问题

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河北师范大学学报（自然科学版） 2011年第5期35卷 438-441页

作者：霍丽芳赵燕冰河北建筑工程学院数理系河北张家口075000 张家口职业技术学院基础部河北张家口075051

利用酉空间上全迷向子空间的性质及计数定理在酉空间上研究了全迷向子空间的Critical问题,得到了相应的计数公式和Critical指数.

关键词：酉空间 Critical指数面格 Mbius函数

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