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Mycobacterium tuberculosis latency-associated antigen Rv1733c SLP improves the accuracy of differential diagnosis of active tuberculosis and latent tuberculosis infection

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Chinese Medical Journal 2022年第1期135卷 63-69页

作者： lifan zhang Huimin Ma Shijun Wan Yueqiu zhang Mengqiu Gao Xiaoqing Liu Division of Infectious Diseases Department of Internal MedicineState Key Laboratory of Complex Severe and Rare DiseasePeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Clinical Epidemiology Unit Peking Union Medical CollegeInternational Clinical Epidemiology NetworkBeijing 100730China Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Tuberculosis Beijing Chest HospitalCapital Medical University/Beijing Tuberculosis and Thoracic Tumor Research InstituteBeijing 101149China

Background: Differential diagnosis of active tuberculosis (ATB) and latent tuberculosis infection (LTBI) has been a challenge for clinicians in high TB burden countries. The purpose of this study was to improve the accuracy of differential diagnosis of ATB and LTBI by using fluorescent immunospot (FluoroSpot) assay to detect specific Th1 cell immune responses. The novelmycobacterium tuberculosis (MTB) latency-associated antigens Rv1733c and synthetic long peptides derived from Rv1733c (Rv1733c SLP) were used based on virulence factors early secreting antigen target-6 (ESAT-6) and culture filtrate protein-10 (CFP-10).Methods: Fifty-seven ATB cases, including 20 pathogen-confirmed ATB and 37 clinically diagnosed ATB, and 36 LTBI cases, were enrolled between January and December 2017. FluoroSpot assay was used to detect the interferon γ (IFN-γ) and interleukin 2 (IL-2) secreted by the specific T cells after being stimulated with MTB virulence factors ESAT-6 and CFP-10, MTB latency-associated antigens Rv1733c and Rv1733c SLP. The receiver operating characteristic (ROC) curve was used to define the best cutoff value of latency-associated antigens in the use of differentiating ATB and LTBI. The sensitivity, specificity, predictive value, and likelihood ratio of ESAT-6 and CFP-10-FluoroSpot combined with latency-associated antigen in the differential diagnosis of ATB and LTBI were also calculated.Results: Following the stimulation with Rv1733c and Rv1733c SLP, the frequency of single IL-2-secreting T cells stimulated by Rv1733c SLP had the largest area under the ROC curve, which was 0.766. With a cutoff value of 1 (spot-forming cells [SFCs]/2.5 × 105 peripheral blood mononuclear cells) for frequency, the sensitivity and specificity of distinguishing ATB from LTBI were 72.2% and 73.7%, respectively. ESAT-6 and CFP-10-FluoroSpot detected the frequency and proportion of single IFN-γ-secreting T cells;the sensitivity and specificity of distinguishing ATB from LTBI were 82.5% and 66.7%, respectively. Combined with the freque

关键词： Mycobacterium tuberculosis latency-associated antigens Active tuberculosis Latent tuberculosis infection Differential diagnosis

Cytomegalovirus antigen-specific T cell immune responses in patients with autoimmune diseases under different cytomegalovirus infection status

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Chinese Medical Journal 2023年第19期136卷 2386-2388页

作者： Yuting Tan Huimin Ma Xiaoqing Liu Xiaochun Shi Wenjie Zheng Jingtao Cui lifan zhang Yaling Dou Baotong Zhou Department of Infectious Diseases Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Center for Tuberculosis Research Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Clinical Epidemiology Unit Peking Union Medical CollegeInternational Clinical Epidemiology NetworkBeijing 100730China Peking Union Medical College Hospitals Center for the Research of Translational Medicine Beijing 100730China Department of Infectious Diseases State Key Laboratory of Complex Severe and Rare DiseasePeking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Rheumatology and Clinical Immunology Key Laboratory of Rheumatology and Clinical ImmunologyPeking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China Department of Clinical Laboratories Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

To the Editor:Patients with autoimmune diseases have increased risk of active cytomegalovirus(CMV)infection due to immune dysfunction or the application of glucocorticoid and immunosuppressants.[1]Cui et al[2]found that compared with human immunodeficiency virus(HIV)/acquired immune deficiency syndrome(27%),posttransplant patients(14.8%),and hematological malignancies(5.1%),patients with autoimmune disease had the highest incidence of CMV antigenemia(35.1%).Monitoring CMV antigen-specific T cell immunity is helpful for understanding the protective immunity against CMV infection and identifing the risk of CMV-related complications.Currently,the CMV antigen-specific T cell immune responses in patients with autoimmune diseases remains unknown.

关键词： immunity patients autoimmune

Biliary spontaneous dislodgement spiral stent for short-term biliary drainage in patients undergoing complete stone removal: A randomized controlled study

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中华医学杂志(英文版) 2024年第11期137卷 2024 Apr 24页

作者： Ye Liansong zhang lifan Mou Yi Yuan Xianglei Liu Wei Wu Chuncheng Luo Yonghong Du Jiang Bai Shuai Xie Jia zhang Qiongying Tan Qinghua Liu Ling Hu Bing Department of Gastroenterology and Hepatology West China Hospital Med-X Center for Materials Sichuan University Chengdu Sichuan China Digestive Endoscopy Medical Engineering Research Laboratory West China Hospital Sichuan University Chengdu Sichuan China West China School of Nursing Sichuan University Chengdu Sichuan China

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基于情绪反弹的网络情绪爆发机制研究

软件工程 2024年第7期27卷 33-41页

作者：杨凯翔张立凡苏畅南京邮电大学管理学院江苏南京210003 昆士兰大学农业与食品可持续发展学院昆士兰州布里斯班4072

网络情绪失调易滋生网络暴力,研究网络情绪的演化规律并识别出关键的影响因素,有利于科学有效地防止网络暴力事件的发生。基于此,文章提出一种基于情绪反弹的网络情绪爆发机制。首先,提出亚稳情绪状态,引入心理敏感系数、官方媒体和意... 详细信息

网络情绪失调易滋生网络暴力,研究网络情绪的演化规律并识别出关键的影响因素,有利于科学有效地防止网络暴力事件的发生。基于此,文章提出一种基于情绪反弹的网络情绪爆发机制。首先,提出亚稳情绪状态,引入心理敏感系数、官方媒体和意见领袖影响力用于诠释情绪转换的概率,建立基于情绪反弹的网络情绪传播模型;其次,求解系统平衡点以证明其稳定性,通过MATLAB仿真对影响指标进行对比分析;最后,选取“张小泉事件”进行案例分析,以验证模型的有效性。实验挖掘出了网络情绪传播的“三个阶段,两个触发点”,为防止情绪反弹和舆情二次爆发提供了参考依据。

关键词：传染病模型情绪反弹亚稳情绪情绪爆发网络暴力

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综合护理在小儿腹部增强CT检查中的应用效果研究

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中国医药指南 2024年第8期22卷 42-44页

作者：吴燕平张丽凡陈艳琛福建省妇幼保健院影像科福州350001 福建省妇幼保健院门诊福州350001

目的探讨综合护理对小儿腹部增强CT检查中的护理价值。方法在福建省妇幼保健院2020年1月至2021年1月入院的患儿中,对小儿腹部增强CT检查病例资料进行回顾性分析,收集不同护理方法下的相关信息,观察组属于综合护理,而对照组为常规护理... 详细信息

目的探讨综合护理对小儿腹部增强CT检查中的护理价值。方法在福建省妇幼保健院2020年1月至2021年1月入院的患儿中,对小儿腹部增强CT检查病例资料进行回顾性分析,收集不同护理方法下的相关信息,观察组属于综合护理,而对照组为常规护理。对两组患者不良反应发生率、护理质量评分、CT检查图像差发生率、护理总满意度指标进行比较。结果观察组患儿护理后发生不良反应发生率、CT检查图像差发生率均低于对照组(均P <0.05);观察组护理人员护理质量评价总分、护理质量满意度均高于对照组(均P <0.05)。结论小儿腹部增强CT检查可在综合护理辅助下,提升护理质量,建立和谐的医患关系,增强患儿检查中的舒适度,提高护理满意度。

关键词：小儿腹部增强CT检查综合护理护理质量

A genome-wide landscape of mRNAs,lncRNAs,and circRNAs during subcutaneous adipogenesis in pigs

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Journal of Animal Science and Biotechnology 2019年第1期10卷 43-55页

作者： Xin Liu Kaiqing Liu Baosen Shan Shengjuan Wei Dongfeng Li Haiyin Han Wei Wei Jie Chen Honglin Liu lifan zhang College of Animal Science and Technology Nanjing Agricultural University College of Life Sciences and Food Engineering Hebei University of Engineering

Background: Preadipocyte differentiation plays a critical role in subcutaneous fat deposition in pigs.However,the roles of different RNAs,such as messenger RNAs(mRNAs),long non-coding RNAs(lncRNAs),and circular RNAs(circRNAs) in the differentiation process of subcutaneous preadipocytes,are still largely unclear.In the present study,a transcriptome analysis,including the analysis of mRNAs,lncRNAs,and circRNAs,during different differentiation stages,namely,day 0(D0),day 2(D2),day 4(D4),and day 8(D8),of subcutaneous preadipocytes from Chinese Erhualian pigs was performed.Results: A total of 1554,470,1344,1777,and 676 differentially expressed(DE) mRNAs,112,58,95,136,and 93 DE lncRNAs,and 902,787,710,932,and 850 DE circRNAs were identified between D2 and D0,between D4 and D2,between D8 and D4,between D4 and D0,and between D8 and D0,respectively.Furthermore,functional enrichment analysis showed that the common DE mRNAs during the entire differentiation process were mainly involved in lipid metabolic and cell differentiation processes.Additionally,co-expression network analysis identified the potential lncRNAs related to adipogenesis,e.g.,MSTRG.131380 and MSTRG.62128.Conclusions: Our study provides new insights of the expression changes of RNAs during adipogenic differentiation,which might contribute to the phenotype of subcutaneous adipogenesis.These results greatly improve our understanding of the molecular mechanisms regulating subcutaneous fat deposition in pigs.

关键词： CircRNA LncRNA mRNA Pig Preadipocyte differentiation RNA-Seq

油菜抗根肿病品种在云南省西南发病区的田间评价

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农学学报 2024年第2期14卷 16-22页

作者：赵凯琴张立帆张云云刘珏罗佳杰杨兆春罗延青云南省农业科学院经济作物研究所昆明650051 德宏州农业技术推广中心云南德宏679200 临翔区农业技术推广站云南临翔677000 腾冲市农业技术推广中心云南腾冲679100

通过明确‘华双5R’和‘华油杂62R’2个抗根肿病油菜品种的田间抗性、农艺性状和产量情况,为油菜根肿病重发区筛选出优良的抗病品种。本研究在云南省西南地区的腾冲市、梁河县和临翔县3个油菜根肿病病区进行田间试验,采用本课题组研发... 详细信息

通过明确‘华双5R’和‘华油杂62R’2个抗根肿病油菜品种的田间抗性、农艺性状和产量情况,为油菜根肿病重发区筛选出优良的抗病品种。本研究在云南省西南地区的腾冲市、梁河县和临翔县3个油菜根肿病病区进行田间试验,采用本课题组研发品种和当地主推品种作为对照。田间试验结果显示,‘华双5R’和‘华油杂62R’在3个试验点均能正常成熟安全越冬,生育期176~199d,各生育期表现整齐一致;‘华双5R’和‘华油杂62R’在腾冲和梁河点对油菜根肿病表现为高度抗病,在临翔点表现为抗病;‘华双5R’和‘华油杂62R’在3个试验点的农艺性状良好;‘华双5R’的产量在3个试验点与7个对照品种相比,比梁河点的‘云油杂12号’减产2.71%,与腾冲点的‘云油杂12号’和‘杂1256’、梁河点的‘云油杂15’和‘花油8号’、临翔点的‘云油杂12号’和‘德油1号’相比增产幅度为7.49%~56.83%;‘华油杂62R’的产量在3个试验点与7个对照品种相比,比梁河点的‘云油杂12号’减产5.76%,比临翔点的‘德油1号’减产4.61%,与腾冲点的‘云油杂12号’和‘杂1256’、梁河点的‘云油杂15’和‘花油8号’、临翔点的‘云油杂12号’相比增产幅度为4.12%~51.90%。‘华双5R’和‘华油杂62R’在3个试验点均能正常成熟,生育期176~199d,对根肿病表现出较强抗性,同时农艺性状良好、产量较高,可在云南省西南地区油菜根肿病病区推广种植。

关键词：油菜根肿病抗性鉴定抗性品种田间栽培

An Exploration of Translation Strategies of Global Cosmetics Brands Based on Reception Aesthetics

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Sino-US English Teaching 2023年第10期20卷 417-422页

作者： zhang lifan ZENG Xianghong University of Shanghai for Science and Technology ShanghaiChina

With the advancement of economic globalization,the flow of commodities from one country to another has become closer and closer.Especially in recent years,the logistics industry has been developing rapidly,overseas purchasing agents and cross-border online shopping have been enormously prevailing,and accordingly more and more British and American cosmetics expect to occupy a place in the huge market of China.Therefore,it is particularly important to explore the Chinese translation strategies for British and American cosmetic brands.Based on the theory of reception aesthetics,this thesis takes the corpus Estee Lauder,La mer,and other famous cosmetics brands.The thesis proposes several methods for translating cosmetic brands:transliteration for proper names like person or geography names;free translation to recreate brand aesthetics;creative translation for new brands;and adapted translation for divergence in language habits.Therefore,this thesis is of theoretical and practical significance as far as the field of cosmetics is concerned.

关键词： cosmetic brands reception aesthetics expectation horizon creative translation

成人择期手术患者全身麻醉健康教育的最佳证据总结

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中华现代护理杂志 2024年第10期30卷 1329-1335页

作者：刘幸马涛洪王亚莉冯可欣张利帆李佩佩山西医科大学护理学院太原030001 山西医科大学第一医院手术供应室太原030001 西安交通大学第一附属医院感染科西安710061

目的检索、评价并整合成人择期手术患者全身麻醉健康教育的最佳证据,为临床健康教育指导提供依据。方法根据PIPOST模式确立循证问题,检索Cochrane Library、BMJ Best Practice、UpToDate、美国国立指南库、国际指南协作网、英国国家卫... 详细信息

目的检索、评价并整合成人择期手术患者全身麻醉健康教育的最佳证据,为临床健康教育指导提供依据。方法根据PIPOST模式确立循证问题,检索Cochrane Library、BMJ Best Practice、UpToDate、美国国立指南库、国际指南协作网、英国国家卫生与临床优化研究所、临床指南库、PubMed、中国知网、万方数据库、医脉通、美国麻醉医师协会、中华医学会麻醉学分会等数据库或专业学会中有关成人择期手术患者全身麻醉健康教育的所有证据。检索时限为建库至2023年6月18日。由2名研究者对纳入的文献进行筛选、质量评价,并对证据进行提取、整合,形成成人择期手术患者全身麻醉健康教育最佳证据。结果共纳入文献10篇,包括4篇指南、3篇临床决策、3篇专家共识。提取出成人择期手术患者全身麻醉健康教育的证据41条,包括麻醉概述、麻醉前评估、麻醉前准备、麻醉过程及配合、麻醉恢复期管理、术后管理6个方面。结论本研究总结了成人择期手术患者全身麻醉健康教育的最佳证据,能够为麻醉健康教育者进行全面、系统的教育提供依据。

关键词：成年人全身麻醉健康教育证据总结循证护理