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Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation

Nano-Micro Letters 2020年第12期12卷 404-420页

作者： Laurens Raes Stephan Stremersch Juan CFraire Toon Brans Glenn Goetgeluk Stijn De Munter lien van hoecke Rein Verbeke Jelter van Hoeck Ranhua Xiong Xavier Saelens Bart vandekerckhove Stefaan De Smedt Koen Raemdonck Kevin Braeckmans Laboratory of General Biochemistry&Physical Pharmacy Ghent University9000 GhentBelgium Cancer Research Institute Ghent(CRIG) 9000 GhentBelgium Department of Diagnostic Sciences Ghent University9000 GhentBelgium VIB-UGent Center for Medical Biotechnology 9052 GhentBelgium Department of Biomedical Molecular Biology Ghent University9000 GhentBelgium Department of Biochemistry and Microbiology Ghent University9000 GhentBelgium

Efficient and safe cell engineering by transfection of nucleic acids remains one of the long-standing hurdles for fundamental biomedical research and many new therapeutic applications,such as CAR T cell-based *** has recently gained increasing attention as a more safe and versatile alternative tool over viral-or DNA transposon-based approaches for the generation of adoptive T ***,limitations associated with existing nonviral mRNA delivery approaches hamper progress on genetic engineering of these hard-to-transfect immune *** this study,we demonstrate that gold nanoparticle-mediated vapor nanobubble(VNB)photoporation is a promising upcoming physical transfection method capable of delivering mRNA in both adherent and suspension *** transfection experiments on HeLa cells showed the importance of transfection buffer and cargo concentration,while the technology was furthermore shown to be effective for mRNA delivery in Jurkat T cells with transfection efficiencies up to 45%.Importantly,compared to electroporation,which is the reference technology for nonviral transfection of T cells,a fivefold increase in the number of transfected viable Jurkat T cells was ***,our results point toward the use of VNB photoporation as a more gentle and efficient technology for intracellular mRNA delivery in adherent and suspension cells,with promising potential for the future engineering of cells in therapeutic and fundamental research applications.

关键词： Transfection mRNA Photoporation Optoporation Gold nanoparticles Vapor nanobubbles

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Globalisation,Europeanisation & Legal Education

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中国法学教育研究 2007年第1期 29-38,258页

作者： Mark van hoecke the European Academy of Legal Theory

Legal education in its current shape mainly developed in the 19th and 20th centuries,within the context of the centralised Nation *** this period law in Europe was almost exclusively domestic law and further mainly private *** the map is quite *** law became clearly more important in different shapes:new areas such as environmental law came into being,more traditional domains such as rights considerably enlarged in scope,and large parts of private law have been intruded by public law.

关键词： Europeanisation Legal education law

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Crack identification in functionally graded material framed structures using stationary wavelet transform and neural network

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2021年第8期22卷 657-671页

作者： Nguyen Tien KHIEM Tran van lien Ngo Trong DUC Institute of Mechanics Vietnam Academy of Science and TechnologyHanoi 10072Vietnam Faculty of Building and Industrial Construction National University of Civil EngineeringHanoi 11600Vietnam Fujita Corporation Hanoi 11000Vietnam

In this paper,an integrated procedure is proposed to identify cracks in a portal framed structure made of functionally graded material(FGM)using stationary wavelet transform(SWT)and neural network(NN).Material properties of the structure vary along the thickness of beam elements by the power law of volumn *** are assumed to be open and are modeled by double massless springs with stiffness calculated from their *** dynamic stiffness method(DSM)is developed to calculate the mode shapes of a cracked frame structure based on shape functions obtained as a general solution of vibration in multiple cracked FGM Timoshenko *** SWT of mode shapes is examined for localization of potential cracks in the frame structure and utilized as the input data of NN for crack depth *** integrated procedure proposed is shown to be very effective for accurately assessing crack locations and depths in FGM structures,even with noisy measured mode shapes and a limited amount of measured data.

关键词： Crack identification Functionally graded material(FGM) Neural network(NN) Stationary wavelet transform(SWT) Dynamic stiffness method

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A comparative analysis of weight-based machine learning methods for landslide susceptibility mapping in Ha Giang area

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Big Earth Data 2023年第4期7卷 1005-1034页

作者： Thanh Trinh Binh Thanh Luu Trang Ha Thi Le Duong Huy Nguyen Trong van Tran Thi Hai van Nguyen Khanh Quoc Nguyen lien Thi Nguyen Faculty of Computer Science Phenikaa UniversityYen NghiaHa DongHanoi 12116Vietnam Phenikaa Research and Technology Institute(PRATI) A&A Green Phoenix Group JSCNo.167 Hoang NganTrung HoaCau GiayHanoi 11313Vietnam Northwestern Geological Division General Department of Geology and Minerals of VietnamHanoiVietnam Youth union office Thuyloi University175 Tay SonDong DaHanoiVietnam Center of Remote Sensing and Geohazards Vietnam Institute of Geosciences and Mineral ResourcesHanoiVietnam

Landslide susceptibility maps (LSMs) are very crucial for planningpolicies in hazardous areas. However, the accuracy and reliability ofLSMs depend on available data and the selection of suitable *** study is conducted to produce LSMs by combinations of machinelearning methods and weighting techniques for Ha Giang province,Vietnam, where has limited data. In study area, we gather 11 landslideconditioning factors and establish a landslide inventory *** the weights of classes (or factors) is very important toprepare data for machine learning methods to generate LSMs. Wefrst use frequency ratio (FR) and analytic hierarchy process (AHP)techniques to generate the weights. Then, random forest (RF), supportvector machine (SVM), logistic regression (LR), and AHP methods arecombined with FR and AHP weights to yield accurate and reliableLSMs. Finally, the performance of these methods is evaluated by fivestatistical metrics, ROC and R-index. The empirical results have shownthat RF is the best method in terms of R-index and the five metrics, *** rate (0.9661), FP rate (0.0), ACC (0.9835), MAE (0.0046), and RMSE(0.0350) for this study area. This study opens the perspective of weightbasedmachine learning methods for landslide susceptibility mapping.

关键词： Landslide logistic regression random forest SvM Vietnam

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A Study on the Selection of the Logistics Centre Location—Vietnam-Based Logistics Sector

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Management Studies 2020年第2期8卷 121-127页

作者： JSC Sai Gon Hiep Phuoc Port Ho Chi Minh City Viet Nam Ho Thi Thu Hoa Nguyen van Khoang Bui Thi Bich lien Tran Quang Dao To Thi Hang Le van Thanh To Thi Hang Ho Chi Minh City University of Transport Ho Chi Minh CityViet Nam JSC Sai Gon Hiep Phuoc Port Ho Chi Minh CityViet Nam

Logistics can be considered as the major drive of the *** centres play a significant role in the entire logistics networks from the point of materials sourcing to the point of last mile *** efficiency of a logistics network depends on the existence of logistics centres to connect and integrate entire logistics *** article discusses the importance of selection of the logistics centre location and recommends the procedure to specify the appropriate logistics centre location as a case of Vietnam-based logistics sector.

关键词： logistics centre Vietnam Ho Chi Minh City location

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Tungsten-based nanocatalysts with different structures for visible light responsive photocatalytic degradation of bisphenol A

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Journal of Environmental Sciences 2024年第5期139卷 569-588页

作者： Hai Bang Truong Thi Thu Loan Doan Nguyen Tien Hoang Nguyen van Tam Minh Kim Nguyen Le Gia Trung Jin Seog Gwag Nguyen Tien Tran Optical Materials Research Group Science and Technology Advanced InstituteVan Lang UniversityHo Chi Minh CityViet Nam Faculty of Applied Technology School of TechnologyVan Lang UniversityHo Chi Minh CityViet Nam The University of Da Nang University of Science and Technology54 Nguyen Luong BangDa NangViet Nam The University of Da Nang University of Science and Education459 Ton Duc Thang St.Lien ChieuDa Nang 550000Viet Nam Institute of Veterinary Science and Technology 31ha zoneTrau QuyGia LamHa Noi 12400Viet Nam Department of Physics Yeungnam UniversityGyeongsanGyeongbuk 38541Republic of Korea Center for Advanced Chemistry Institute of Research and DevelopmentDuy Tan University03 Quang TrungDa Nang 550000Viet Nam Faculty of Natural Sciences Duy Tan University03 Quang TrungDa Nang 550000Viet Nam

Environmental pollution,such as water contamination,is a critical issue that must be absolutely ***,three different morphologies of tungsten-based photocatalysts(WO_(3)nanorods,WO_(3)/WS_(2)nanobricks,WO_(3)/WS_(2)nanorods)are made using a simple hydrothermal method by changing the solvents(H_(2)O,DMF,aqueous HCl solution).The as-prepared nanocatalysts have excellent thermal stability,large porosity,and high *** results show all materials have good photocatalytic activity in aqueous media,with WO_(3)/WS_(2)nanorods(NRs)having the best activity in the photodegradation of bisphenol A(BPA)under visible-light *** may originate from increased migration of charge carriers and effective prevention of electron–hole recombination in WO_(3)/WS_(2)NRs,whereby this photocatalyst is able to generate more reactive·OH and·O_(2)^(–)species,leading to greater photocatalytic *** 99.6% of BPA is photodegraded within 60 min when using 1.5 g/L WO_(3)/WS_(2)NRs and 5.0 mg/L BPA at pH ***,the optimal conditions(pH,catalyst dosage,initial BPA concentration)for WO_(3)/WS_(2)NRs are also elaborately *** rod-like heterostructures are expressed as potential catalysts with excellent photostability,efficient reusability,and highly active effectivity in different types of *** particular,the removal efficiency of BPA by WO_(3)/WS_(2)NRs reduces by only 1.5% after five recycling runs and even reaches 89.1%in contaminated lake *** study provides promising insights for the nearly complete removal of BPA from wastewater or different water resources,which is advantageous to various applications in environmental remediation.

关键词： Photodegradation Bisphenol A Tungsten-based nanocatalyst Structure Nanorod

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SOME PRELIMINARY RESULTS FROM STUDY OF CRUSTAL DEFORMATION IN VIETNAM

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大地测量与地球动力学 2003年第B12期23卷 114-118页

作者： Cao Dinh Trieu Le van Dung Nguyen Huu Tuyen Pham Nam Hung Institute of Geophysics Institute of GeophysicsInstitute of GeophysicsInstitute of Geophysics 18 Hoang Quoc Viet Road Cau GiayHanoi18 Hoang Quoc Viet Road Cau GiayHanoi18 Hoang Quoc Viet Road Cau GiayHanoi18 Hoang Quoc Viet Road Cau GiayHanoi

In this work, the authors present some results of strain monitoring within the territory of Vietnam from 1984 to 2001. Data analysis shows that (1) the Phu lien area is tectonically stable with low strain rate, 1×10\+\{-6\} /a; (2) the Hoa Binh area has indication of tectonic instability with relatively high strain rate of the earth crust: absolute value of the strain reaching 100×10\+\{-6\}, usual average strain amplitude of 60×10\+\{-6\}, whereas the monthly average strain intensity varies in a range from 30×10\+\{-6\} to 50×10\+\{-6\}; (3) there are clear indications of the strain state of the earth crust before occurrence of earthquakes of medium or higher magnitude. This is a very important indication in the research and short term prediction of earthquakes, especially for the areas with low seismic stability like the NW of Vietnam.

关键词：越南表层变形应变测量系统操作原则应变数据因变与地震相对关系地震活动特性

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软木塞基碳量子点的水热合成及其生物相融荧光成像应用

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新型炭材料 2022年第3期37卷 595-602页

作者： Quang Ngo Khoa Hieu Nguyen Ngoc Bao Vo van Quoc Phuoc Vo Thi Ngoc Le Xuan Diem Doc Luong Quang Tri Nguyen Minh Son Le Vu Truong Son Le van Thanh Ha Che Thi Cam University of Sciences Hue University77 Nguyen HueHueVietnam Faculty of Environmental and Natural Sciences Duy Tan UniversityDa Nang550000Vietnam Institute for Research and Training in Medicine Biology and PharmacyDuy Tan UniversityDa Nang550000Vietnam University of Agriculture and Forestry Hue University102 Phung HungHueVietnam University of Science and Education The University of Da Nang459 Ton Duc ThangLien ChieuDa NangVietnam

以废弃酒瓶软木塞为原料,采用低成本、简单的水热方法合成碳量子点。通过TEM、FTIR、Raman、UV-Vis、PL光谱对碳量子点的结构和光学性能进行分析表征。结果表明,碳量子点的平均直径为6.2±2.7 nm,PL激发谱和碳量子点表面的官能团有关。... 详细信息

以废弃酒瓶软木塞为原料,采用低成本、简单的水热方法合成碳量子点。通过TEM、FTIR、Raman、UV-Vis、PL光谱对碳量子点的结构和光学性能进行分析表征。结果表明,碳量子点的平均直径为6.2±2.7 nm,PL激发谱和碳量子点表面的官能团有关。用硫酸奎宁作为参考,碳量子点的量子效率为1.54%。将获得的碳量子点应用在骨髓间充质干细胞的细胞生物成像上,发现用碳量子点处理后,骨髓间充质干细胞分别在320~380 nm、450~490 nm和450~490 nm范围显示绿色、黄色和红色荧光,表明了碳量子点在荧光成像领域具有潜在的应用价值。

关键词：碳点水热合成荧光荧光图像废酒瓶塞

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我国古代自动装置的原理分析及其成就的探讨

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自动化学报 1965年第2期 57-65页

作者：万百五

本文论证了约在两千年以前我们祖先所发明和使用的指南车,是一个按扰动调节原理工作的开环自动调节系统。北宋时代(九百年以前)的苏颂和韩公廉,在他们创造发明的水运仪象台里,使用了一个天衡装置,它是一个按被调量的偏差进行调节的自动... 详细信息

本文论证了约在两千年以前我们祖先所发明和使用的指南车,是一个按扰动调节原理工作的开环自动调节系统。北宋时代(九百年以前)的苏颂和韩公廉,在他们创造发明的水运仪象台里,使用了一个天衡装置,它是一个按被调量的偏差进行调节的自动调节器。文中探讨了这两个系统的结构图和传递函数。此外,本文还介绍了铜壶滴漏装置和水运仪象台枢轮转速恒定系统,它们都是参数恒定系统,能够自动补偿其些外界扰动作用对于被调量的影响。本大最后分析研究了我国古代各种类型的自动装置,认为可以将它们划分为自动检测与自动传送、开环自动控制、开环或闭环自动调节、开环程序控制、参数恒定系统和模拟研究等六类,从而论断:与其他国家相比,我国古代在自动学方面的成就是非常突出的。

关键词：水运仪象台装置自动控制原理分析天衡我国古代方框图开环

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AB_2和AB_3型分子键角变化的规律

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厦门大学学报（自然科学版） 1961年第1期25卷 68-73页

作者：张乾二林连堂王南钦王银桂厦门大学化学系

本文对AB_2和AB_3型分子的几何模型变化的规律进行探讨。依关于非(?)合原子间的相互作用是决定键角偏离“正常值”的主要因素这观点,得出一个联系键长、键角和非键合原子的范德华半径的关系的经验公式,这经验公式表明: (1)非键合原子间... 详细信息

本文对AB_2和AB_3型分子的几何模型变化的规律进行探讨。依关于非(?)合原子间的相互作用是决定键角偏离“正常值”的主要因素这观点,得出一个联系键长、键角和非键合原子的范德华半径的关系的经验公式,这经验公式表明: (1)非键合原子间的相互作用是决定分子空间构型的一个重要因素。 (2)非键合原子的电子云相互重叠程度决定于“中心原子”的性质。

关键词：中心原子分子电子云密度 AB3 键角化学键角变化

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