检索结果-南通市图书馆

Clinical significance of microRNA-130b in osteosarcoma and its role in cell growth and invasion

Asian Pacific Journal of Tropical Medicine 2015年第9期8卷 737-741页

作者： lie-dao yu Ri-Long Jin Peng-Cheng Gu Zhi-Heng Ling Xiang-Jin Lin Jing-yu Du Department of Orthopedics the First Affiliated Hospital of Zhejiang University

Objective: To investigate clinical signii cance of microRNA-130b(mi R-130b) in osteosarcoma and its role in cell growth and invasion. Methods: miR-130b expression was detected in 68 samples of surgically resected osteosarcoma and matched normal tumor-adjacent tissues by q RT-PCR. The expression of miR-130b was altered by corresponding vectors in osteosarcoma cells, and then Western blot was used to detect the expression of PPAR毭. Brd U cell proliferation and Transwell assays were performed to determine cell proliferation and invasion. Results: The expression of miR-130b in osteosarcoma tissues was signii cantly higher than that in normal tumor-adjacent tissues. Its expression in patients with metastasis was signii cantly higher than that in those without metastases. miR-130b expression in tumor tissues was signii cantly associated with tumor size, clinical stage and distant metastasis. And its expression was signii cantly correlated with overall survival and disease free survival. miR-130b overexpression obviously repressed the expression of PPAR毭, and resulted in signii cant increase of Saos-2 cell proliferation and invasion. On the contrast, repressing miR-130b expression with its inhibitor signii cantly increased PPAR毭 expression, and inhibited MG-63 cell proliferation and invasion. Conclusions: The high-expression of miR-130b is correlated with the adverse clinicopathological features and poor prognosis in osteosarcoma. miR-130b may regulate proliferation and invasion of osteosarcoma cells by targeting PPAR毭, suggesting miR-130b may play a key role in the progression of osteosarcoma.

关键词： microRNA-130b Osteosarcoma Tumor growth Invasion Clinical significance

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fungal spondylodiscitis in a patient recovered from H7N9 virus infection:a case study and a literature review of the differences between Candida and Aspergillus spondylodiscitis

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2016年第11期17卷 874-881页

作者： lie-dao yu Zhi-yun FENG Xuan-wei WANG Zhi-heng LING Xiang-jin LIN Department of Orthopedic Surgery the First Affiliated Hospital School of Medicine Zhejiang University

To report a rare case of fungal spondylodiscitis in a patient recovered from H7N9 virus infection and perform a literature review of the different characteristics of Candida and Aspergillus spondylodiscitis, we reviewed cases of spondylodiscitis caused by Candida and Aspergillus species. Data, including patients＇ information, patho- genic species, treatment strategy, outcomes, and relapses, were collected and summarized. The characteristics of Canclida and Aspergillus spondylodiscitis were compared to see if any differences in clinical features, management, or consequences could be detected. The subject of the case study was first misdiagnosed as having a vertebral tumor, and then, following open biopsy, was diagnosed as having fungal spondylodiscitis. The patient made a good recovery following radical debridement. Seventy-seven additional cases of Candida spondylodiscitis and 94 cases of Asper- gillus spondylodiscitis were identified in the literature. Patients with Candida spondylodiscitis tended to have a better outcome than patients with Aspergillus spondylodiscitis （cure rate 92.3% vs. 70.2%）. Candida was found more fre- quently （47.8%） than Aspergillus （26.7%） in blood cultures, while neurological deficits were observed more often in patients with Aspergillus spondylodiscitis （43.6% vs. 25.6%）. Candida spinal infections were more often treated by radical debridement （60.5% vs. 39.6%）. Patients with Candida spondylodiscitis have better outcomes, which may be associated with prompt recognition, radical surgical debridement, and azoles therapy. A good outcome can be ex- pected in fungal spondylodiscitis with appropriate operations and anti-fungal drugs.

关键词： Spondylodiscitis Spinal infection H7N9 virus Avian influenza virus Candida Aspergillus Fungi Review

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Probabilistic Durability Assessment of Concrete Structures in Marine Environments:Reliability and Sensitivity Analysis

引用

China Ocean Engineering 2017年第1期31卷 63-73页

作者： yu Bo NING Chao-lie LI Bing Key Laboratory of Disaster Prevention and Structural Safety of the Ministry of Education ChinaSchool of Civil Engineering&ArchitectureGuangxi UniversityNanning 530004China Shanghai Institute of Disaster Prevention and Relief Tongji UniversityShanghai 200092China Natural Hazard Research Centre(NHRC) Nanyang Technological UniversitySingapore 639798

A probabilistic framework for durability assessment of concrete structures in marine environments was proposed in terms of reliability and sensitivity analysis, which takes into account the uncertainties under the environmental,material, structural and executional conditions. A time-dependent probabilistic model of chloride ingress was established first to consider the variations in various governing parameters, such as the chloride concentration,chloride diffusion coefficient, and age factor. Then the Nataf transformation was adopted to transform the nonnormal random variables from the original physical space into the independent standard Normal space. After that the durability limit state function and its gradient vector with respect to the original physical parameters were derived analytically, based on which the first-order reliability method was adopted to analyze the time-dependent reliability and parametric sensitivity of concrete structures in marine environments. The accuracy of the proposed method was verified by comparing with the second-order reliability method and the Monte Carlo simulation. Finally, the influences of environmental conditions, material properties, structural parameters and execution conditions on the time-dependent reliability of concrete structures in marine environments were also investigated. The proposed probabilistic framework can be implemented in the decision-making algorithm for the maintenance and repair of deteriorating concrete structures in marine environments.

关键词： probabilistic environments durability execution verified maintenance parametric apparent repair Sensitivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A generalized solution of elasto-aerodynamic lubrication for aerodynamic compliant foil bearings

引用

Science China(Technological Sciences) 2005年第4期48卷 414-429页

作者： yu lie QI Shemiao GENG Haipeng Institute of Mechatronics and Information Systems Key Laboratory of Education Ministry for Modern Design and Rotor-Bearing SystemsXi'an Jiaotong UniversityXi'an 710049China

Although aerodynamic compliant foil bearings are successfully applied in a number of turbo-machineries,theoretical researches on the modeling,performance prediction of compliant foil bearings and the dynamic analysis of the related rotor system seem still far behind the experimental investigation because of structural complexity of the foil bearings.A generalized solution of the elasto-aerodynamic lubrication is presented in this paper by introducing both static and dynamic deformations of foils and solving gas-lubricated Reynolds equations with deformation equations *** solution can be used for the calculation of dynamic stiffness and damping,as well as the prediction of static performances of foil *** theories and methods are also presented for the purpose of the prediction of dynamic behavior of a rotor system equipped with foil bearings.

关键词： compliant foil bearing elasto-aerodynamic lubrication dynamic deformation dynamic stiffness and damping

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Stability Analysis on Rotor Systems Supported by Self-acting Tilting-pad Gas Bearings with Frequency Effects

引用

Chinese Journal of Mechanical Engineering 2011年第3期24卷 380-385页

作者： YANG Lihua QI Shemiao GENG Haipeng yu lie Key Laboratory of Ministry of Education for Strength and Vibration Xi ＇an Jiaotong University Xi ＇an 710049 China Dongfang Steam Turbine Corporation Deyang 618000 China School of Aerospace Xi ＇an Jiaotong University Xi ＇an 710049 China Institute of Mechatronics and Information Systems Xi ＇an Jiaotong Universit） Xi ＇an 710049 China

The recent research on stability of gas bearing-rotor systems still mostly adopts the same method as in oil-lubricated bearing-rotor *** dynamic coefficients of gas bearings in the case that the perturbation frequencies are same as the rotating speed are used to carry out the stability analysis of rotor *** method does not contact the frequency characteristics of dynamic stiffness and damping coefficients of gas bearings with the dynamical behaviors of rotor ***,the effects of perturbation frequencies on the stability of systems are not taken into *** this paper,the dynamic stiffness and damping coefficients of tilting-pad gas bearings are calculated by the partial derivative *** the base of solution of dynamic coefficients,two computational models are produced for stability analysis on rotor systems supported by tilting-pad gas bearings according to whether the degrees of the freedom of pads tilting motions are included in the equations of motion or *** the condition of considering the frequency effects of dynamic coefficients of tilting-pad gas bearings,the corresponding eigenvalues of the rigid and first five vibration modes of the system with the working speeds of 8-30 kr/min are computed through iteratively solving the equations of motion of rotor-system by using two computational models,*** to the obtained eigenvalues,the stability of rotor system is *** results indicate that the eigenvalues and the stability of rotor system obtained by these two computational models are well agreement each *** all can more accurately analyze the stability of rotor systems supported by tilting-pad gas *** research has important meaning for perfecting the stability analysis method of rotor systems supported by gas bearings.

关键词： bearing-rotor system tilting-pad bearing logarithm attenuation rate stability analysis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

UNBALANCE RESPONSE AND TOUCH-RUBBING THRESHOLD SPEED OF ROTOR SUBJECTED TO NONLINEAR MAGNETIC FORCES

引用

Chinese Journal of Mechanical Engineering 2008年第2期21卷 1-4页

作者： JING Minqing LI Zixin LUO Min yu lie College of Mechanical Engineering Xi＇an Jiaotong University Xi＇an 710049 China

Because of the effect of unbalance excitation and nonlinear magnetic force, the large vibration of the rotor supported by active magnetic bearing（AMB） will go beyond the radial gap of the bearing, even causing mechanical touch-rubbing when the system works at an operational speed closer to the critical speed. In order to investigate this problem, the linear model and nonlinear model of the single mass symmetric rigid rotor system supported by AMB are established respectively and the corresponding transfer functions of close-loop system are given. To pass through the numerical calculation by using MATLAB/Simulink, the effect of both the unbalance response and threshold speed of touch-rubbing of the system subjected to nonlinear magnetic forces and nonlinear output current of power amplifier are studied. Furthermore, threshold speed of touch-rubbing of the rotor-bearing system is defined and the results of numerical simulation are presented. Finally, based on above studies, two methods of increasing the touch-rubbing threshold speed are discussed.

关键词： Active magnetic bearing (AMB) Vibration Non-linearity Touch-rubbing speed

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Pressure dynamics of asymmetrically fractured wells in an arbitrarily shaped reservoir

引用

Journal of Hydrodynamics 2019年第4期31卷 767-777页

作者： yu-long Zhao Bao-chao Shan lie-hui Zhang State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum University Chengdu 610500 China State Key Laboratory^ of Coal Combustion Huazhong University^ of Science and Technology Wuhan 430074 China

A numerical model is established and with the model, the boundary element method and the coupled boundary/finite element method are used to analyze the pressure dynamics of an asymmetrically fractured well in an arbitrarily shaped reservoir. The equation is solved in the Laplace domain, with different flow regimes with or without considerations of the wellbore storage and skin effects. The parameter sensitivity analyses, including the asymmetry factor, the fracture conductivity, the outer boundary shape, the boundary size and the well location, are conducted. The model is validated by comparing the results with those of analytical solutions of two simplified cases and a field case study. The study can be used for the well testing interpretation and the hydraulic fracturing design.

关键词： Asymmetrically fractured well finite conductivity fracture pressure dynamics numerical method arbitrary drainage area

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Experimental study on the effects of sediment size and porosity on contaminant adsorption/desorption and interfacial diffusion characteristics

引用

Journal of Hydrodynamics 2013年第1期25卷 20-26页

作者： FAN Jing-yu HE Xiao-yan WANG dao-zeng Shanghai Institute of Applied Mathematics and Mechanics Shanghai University

The joint effects of the sediment size and porosity on the contaminant adsorption/desorption and interfacial diffusion characteristics were experimentally investigated. The adsorption of Phosphorus （P） on the natural and artificial sediment suspensions was measured with respect to the P adsorption isotherms and kinetics in the experiment. The obtained adsorption isotherms for different grain-sized sediment suspensions fit well with the Langmuir equation, dependent on the initial aqueous concentration and sediment content. The P kinetic adsorption behaviors for cohesive fine-grained and non-cohesive coarse-grained sediment suspensions clearly show the size-dependent feature. On the other hand, the P kinetic release feature of a porous sediment layer is affected by not only the direct desorption of the uppermost sediments, but also the diffusivity in the pore-water within the underlying sediment layer, characterized by the sediment size and porosity, respectively. Furthermore, the temporal contaminant release from the permeable sediment layer into the overlying water column increases with the increasing flow velocity, while this enhancement in mediating the interfacial diffusion flux is somewhat insignificant in an immediate release stage, largely due to the resistance of the diffusive boundary layer on the hydrodynamic disturbance.

关键词： sediment contaminant adsorption desorption interfacial diffusion

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Low frequency vibration control of railway vehicles based on ahigh static low dynamic stiffness dynamic vibration absorber

引用

Science China(Technological Sciences) 2019年第1期62卷 60-69页

作者： SUN yu GONG dao ZHOU JinSong SUN WenJing XIA ZhangHui Institute of Rail Transit Tongji University

In order to control the low frequency vibration of railway vehicles, a vertical two degrees of freedom(2DOF) low frequency dynamic vibration absorber(DVA) based on acceleration is proposed. Parameters of the dynamic vibration absorber are put forth to control the low frequency vibration of car body bouncing and pitching. Next, the acceleration power spectrum density(PSD)and ride quality of the car body are calculated based on the pseudo excitation method(PEM) and covariance algorithm,respectively. According to the requirement of 2DOF low frequency DVA, the isolators with high static low dynamic stiffness(HSLDS) are designed. A high-speed train dynamic model containing HSLDS isolators is established to validate the effects on the car body vibration. The results reveal that the 2D low frequency DVA can significantly reduce the vibration of the car body bouncing and pitching. Thus, the ride quality of the vehicle is increased, and passenger comfort is improved.

关键词： railway vehicle dynamics ride quality low frequency dynamic vibration absorber high static low dynamic stiffness

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Histone deacetylase HDA710 controls salt tolerance by regulating ABA signaling in rice

引用

Journal of Integrative Plant Biology 2021年第3期63卷 451-467页

作者： Farhan Ullah Qiutao Xu yu Zhao dao-Xiu Zhou National Key Laboratory of Crop Genetic Improvement Huazhong Agriculture UniversityWuhan 430030China Institute of Plant Science of Paris-Saclay(IPS2) University Paris-SaclayCNRSINRAEOrsay 91405France

Plants have evolved numerous mechanisms that assist them in withstanding environmental *** deacetylases(HDACs)play crucial roles in plant stress responses;however,their regulatory mechanisms remain poorly ***,we explored the function of HDA710/OsHDAC2,a member of the HDAC RPD3/HDA1 family,in stress tolerance in rice(Oryza sativa).We established that HDA710 localizes to both the nucleus and cytoplasm and is involved in regulating the acetylation of histone H3 and H4,specifically targeting H4 K5 and H4 K16 under normal ***710 transcript accumulation levels were strongly induced by abiotic stresses including drought and salinity,as well as by the phytohormones jasmonic acid(JA)and abscisic acid(ABA).hda710 knockout mutant plants showed enhanced salinity tolerance and reduced ABA sensitivity,whereas transgenic plants overexpressing HDA710 displayed the opposite ***,ABAand salt-stress-responsive genes,such as OsLEA3,OsABI5,OsbZIP72,and OsNHX1,were upregulated in hda710 compared with wild-type *** expression differences corresponded with higher levels of histone H4 acetylation in gene promoter regions in hda710 compared with the wild type under ABA and salt-stress ***,these results suggest that HDA710 is involved in regulating ABA-and salt-stress-responsive genes by altering H4 acetylation levels in their promoters.

关键词： abscisic acid HDA710 histone acetylation rice salinity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：