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In situ assembly of fibrinogen/hyaluronic acid hydrogel via knob-hole interaction for 3D cellular engineering

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Bioactive materials 2017年第4期2卷 253-259页

作者： Shengjie Huang Chunfen Wang Jingwei Xu lie ma Changyou Gao MOE Key Laboratory of macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027China

Hyaluronic acid(HA)-based hydrogels have applied widely for biomedical applications due to its biocompatibility and ***,the use of initiators or crosslinkers during the hydrogel formation may cause cytotoxicity and thereby impair the *** by the crosslinking mechanism of fibrin gel,a novel HA-based hydrogel was developed via the in situ supramolecular assembly based on knob-hole interactions between fibrinogen and knob-grafted HA(knob-g-HA)in this *** knob-grafted HA was synthesized by coupling knob peptides(GPRPAAC,a mimic peptide of fibrin knob A)to HA via Michael *** the translucent fibrinogen/knob-g-HA hydrogels were prepared by simply mixing the solutions of knob-g-HA and fibrinogen at the knob/hole ratio of *** rheological behaviors of the fibrinogen/knob-g-HA hydrogels with the fibrinogen concentrations of 50,100 and 200 mg/mL were evaluated,and it was found that the dynamic storage moduli(G0)were higher than the loss moduli(G00)over the whole frequency range for all the *** SEM results showed that fibrinogen/knob-g-HA hydrogels presented the heterogeneous mesh-like structures which were different from the honeycomb-like structures of fibrinogen/ma-HA ***,a higher swelling ratio was obtained in the groups of fibrinogen/knob-g-HA ***,the cytocompatibility of fibrinogen/knob-g-HA hydrogels was proved by live/dead stainings and MTT assays in the 293T cells encapsulation *** these results highlight the biological potential of the fibrinogen/knob-g-HA hydrogels for 3D cellular engineering.

关键词： Fibrinogen Knob-hole interactions Hyaluronic acid In situ assembly Cell encapsulation

Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes

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Bioactive materials 2021年第11期6卷 4065-4072页

作者： Yilun Luo Xiaowen Zheng Peiqi Yuan Xingyao Ye lie ma MOE Key Laboratory of macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027China

Due to the critical roles of macrophage in immune response and tissue repair,harnessing macrophage phenotypes dynamically to match the tissue healing process on demand attracted many *** there have developed many advanced platforms with dynamic features for cell manipulation,few studies have designed a dynamic chemical pattern to sequentially polarize macrophage phenotypes and meet the immune requirements at various tissue repair ***,we propose a novel strategy for spatiotemporal manipulation of macrophage phenotypes by a UV-induced dynamic Arg-Gly-Asp(RGD)*** employing a photo-patterning technique and the specific interaction between cyclodextrin(CD)and azobenzene-RGD(Azo-RGD),we prepared a polyethylene glycol-dithiol/polyethylene glycol-norbornene(PEG-SH/PEG-Nor)hydrogel with dynamic RGD-patterned *** irradiation with 365-nm UV light,the homogeneous RGD surface was transformed to the RGD-patterned surface which induced morphological transformation of macrophages from round to elongated and subsequent phenotypic transition from pro-inflammation to *** mechanism of phenotypic polarization induced by RGD pattern was proved to be related to Rho-associated protein kinase 2(ROCK2).Sequential modulation of macrophage phenotypes by the dynamic RGD-patterned surface provides a remote and non-invasive strategy to manipulate immune reactions and achieve optimized healing outcomes.

关键词： Light-responsive Dynamic RGD pattern macrophage phenotype Immune response Tissue repair

Gene-activated dermal equivalents to accelerate healing of diabetic chronic wounds by regulating inflammation and promoting angiogenesis

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Bioactive materials 2020年第3期5卷 667-679页

作者： Dong Lou Yu Luo Qian Pang Wei-Qiang Tan lie ma MOE Key Laboratory of macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027PR China Department of Plastic Surgery Sir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou310016PR China

Diabetic chronic wound,characterized by prolonged inflammation and impaired angiogenesis,has become one of the most serious challenges in clinic and pose a significant healthcare burden *** a great variety of wound dressings have been developed,few of encouraged achievements were obtained so *** this study,the gene-activated strategy was applied to enhance sustained expression of vascular endothelial growth factor(VEGF)and achieve better healing outcomes by regulating inflammation and promoting *** gene-activated bilayer dermal equivalents(Ga-BDEs),which has good biocompatibility,were fabricated by loading the nano-sized complexes of Lipofectamine 2000/plasmid DNA-encoding VEGF into a collagen-chitosan scaffold/silicone membrane bilayer dermal *** DNA complexes were released in a sustained manner and showed the effective transfection capacities to up-regulate the expression of VEGF in *** overcome cutaneous contraction of rodents and mimic the wound healing mechanisms of the human,a reformative rat model of full-thickness diabetic chronic wound was *** the treatment of Ga-BDEs,speeding wound healing was observed,which is accompanied by the accelerated infiltration and phenotype shift of macrophages and enhanced angiogenesis in early and late healing phases,*** proved that Ga-BDEs possess the functions of immunomodulation and pro-angiogenesis ***,the better regeneration outcomes,including deposition of oriented collagen and fast reepithelialization,were *** these results indicated that,being different from traditional pro-angiogenic concept,the up-regulated expression of VEGF by Ga-BDEs in a sustained manner shows versatile potentials for promoting the healing of diabetic chronic wounds.

关键词： Gene-activated dermal equivalent Vascular endothelial growth factor Inflammation Angiogenesis Diabetic chronic wounds

In situ delivery of apoptotic bodies derived from mesenchymal stem cells via a hyaluronic acid hydrogel:A therapy for intrauterine adhesions

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Bioactive materials 2022年第6期7卷 107-119页

作者： Liaobing Xin Cheng Wei Xiaomei Tong Yangyang Dai Dong Huang Jianmin Chen lie ma Songying Zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalSchool of MedicineZhejiang UniversityHangzhou310016China MOE Key Laboratory of macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027China Key Laboratory of Reproductive Dysfunction management of Zhejiang Province.No.3 Qingchun East Road Jianggan DistrictHangzhou310016China

Stem cell-based and stem cell-derived exosome-based therapies have shown promising potential for endometrial regeneration and the clinical treatment of intrauterine adhesions(IUAs).Evidence shows that apoptosis occurs in a majority of grafted stem cells,and apoptotic bodies(ABs)play a critical role in compensatory tissue ***,the therapeutic potential of AB-based therapy and its mechanism have not been explored in ***,a cell-free therapeutic strategy was developed by incorporating mesenchymal stem cell-derived ABs into a hyaluronic acid(HA)hydrogel to achieve endometrial regeneration and fertility ***,we found that the ABs could induce macrophage immunomodulation,cell proliferation,and angiogenesis in *** HA hydrogel promoted the retention of ABs and facilitated their continuous *** a murine model of acute endometrial damage and a rat model of IUAs,in situ injection of the AB-laden HA hydrogel could efficiently reduce fibrosis and promote endometrial regeneration,resulting in the fertility ***,ABs show good potential as therapeutic vesicles,and the AB-laden HA hydrogel appears to be a clinically feasible and cell-free alternative for endometrial regeneration and IUA treatment.

关键词： Apoptotic bodies Mesenchymal stem cells Endometrial regeneration Intrauterine adhesions Tissue engineering

Analysis of macular microvasculature and thickness after ICL implantation in patients with myopia using optical coherence tomography

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1948-1954页

作者： Qiu-Jian Zhu Meng-Yu Wang Peng Yu Xiao-Suo Liang lie ma Hai-Xiang Xiao You Yuan Department of Ophthalmology Lixiang Eye Hospital of Soochow UniversitySuzhou 215021Jiangsu ProvinceChina

AIM:To investigate changes in macular vessels and thickness in myopic eyes after intraocular collamer lens(ICL)implantation using quantitative optical coherence tomography angiography(OCTA).METHODS:This retrospective included 73 myopic eyes of 73 patients(average age,27.53±6.16 y)who underwent ICL implantation(28 eyes were Toric ICL).Axial length(AL),uncorrected visual acuity(UCVA),refractive dioptre(RD),intraocular pressure(IOP)and OCTA were measured and compared with before and 1 wk,1,and 3 mo after *** was used to image vessel density(VD)and skeleton density(SD)in both the superficial(SCP)and deep capillary plexus(DCP).Central retinal thickness(CRT)and ganglion cellinner plexiform layer thickness(GCT)were also *** between pre-and postoperative measurements were analysed by repeated measures analysis of ***:Compared with preoperative data,postoperative data on UCVA revealed significant improvements in all patients(Pmacular area changes that occur after surgery need attention.

关键词： intraocular collamer lens implantation vessel density macular thickness optical coherence tomography angiography

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三维成像载荷共孔径光学系统设计

光学精密工程 2018年第9期26卷 2326-2333页

作者：马烈陈波中国科学院长春光学精密机械与物理研究所吉林长春130033 中国科学院大学北京100049

基于激光雷达高程数据与平面影像数据融合的三维成像技术是三维遥感探测技术的重要发展方向之一。本文设计了基于此体制的共孔径三维成像载荷的光学系统。从受限的空间尺寸出发,以此作为设计输入条件,得到光学系统的初始设计参数,设计... 详细信息

基于激光雷达高程数据与平面影像数据融合的三维成像技术是三维遥感探测技术的重要发展方向之一。本文设计了基于此体制的共孔径三维成像载荷的光学系统。从受限的空间尺寸出发,以此作为设计输入条件,得到光学系统的初始设计参数,设计了焦距为2 400mm,F数为5.33的偏视场同轴三反系统。采用三镜前置的方式,大大缩短了光学系统的轴向长度,使光学系统的轴向长度仅为焦距的1/4.36,在有限的空间内,实现了长焦距、高分辨率的光学系统排布。采用偏视场设计,避免了系统内的二次遮挡。整个系统的成像质量良好,无色差,畸变小,光学调制传递函数接近衍射极限,同时其相对孔径较大,有效通光孔径较大,能量集中度高,在保证高地面分辨率的同时,满足了激光接收端对能量的需求。

关键词：三维成像同轴三反偏视场共孔径三镜前置光学系统设计

An Organic Solvent-free Approach towards PDI/Carbon Cloth Composites as Flexible Lithium Ion Battery Cathodes

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Chinese Journal of Polymer Science 2020年第5期38卷 540-549,I0008页

作者： Dong-Qing Wu Deng Lu Peng Yang lie ma Biao Jiang Xin Xi Fan-Cheng Meng Wen-Bei Zhang Fan Zhang Qian-Qian Zhong Rui-Li Liu School of Chemistry and Chemical Engineering Shanghai Jiao Tong UniversityShanghai200240China School of Electronic Information and Electrical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

An acidic solution based method towards flexible lithium ion battery(LIB)cathodes is developed in this work with perylene diimide(PDI)as the electroactive component and carbon cloth(CC)as the current *** this approach,PDI is firstly dispersed in concentrated sulfuric acid(H2S04)and then deposited on CC substrate after the dilution of H2S04,which provides an organic solvent-free strategy to construct integrated LIB *** acdic solution based fabrication process also allows the facile adjusting of loading amounts of PDI in the cathodes,which can effectively influence the battery performances of the PDI/CC *** the result,the acidic solution processed PDI/CC cathode can deliver a high specific capacity of-36 mah·g^-1 at the current density of 50 ma·g^-1 in both half cell with lithium foil as anode and full cell with pre-lithiated CC as *** both types of the batteries,the PDI/CC cathodes show good cycling stabilities by retaining^84%of the initial capacities after 300 charge-discharge cycles at 500 ma·g^-***,the excellent mechanical stability of the PDI/CC cathode enables the LIBs in pouch cell to maintain the electrochemical performances under various bending states,demonstrating their potentials for flexible LIBs.

关键词： Acidic solution processing Perylene diimide Carbon clothes Organic cathodes Flexible lithium ion batteries

Investigation of Performance of Turret Assembly in Inboard Turret Mooring System

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China Ocean Engineering 1991年第4期6卷 409-418页

作者： ma Peisun , ma lie and Lu Shunshou Associate Professor, Shanghai Jiaotong University, Shanghai Graduate, Shanghai Jiaotong University, Shanghai Engineer, Shanghai Jiaotong University, Shanghai

To fulfil the need of development of offshore oil floating production system, the performance of turret assembly in an inboard turret mooring system has been investigated in the paper. By means of systematic evaluation, the optimal structure of the turret for single-point mooring systems has been determined. Through model tests of a turret mooring system, several groups of data such as forces, moments and displacements under different environmental conditions have been obtained. Stresses and strains of the turret structure have been calculated by means of the finite element method corresponding to the situation in the model experiment. The study shows that the selected turret structure and the designed turret assembly are technically feasible.

关键词： turret mooring system structure calculations finite element method turret assembly

A construct of adipose-derived mesenchymal stem cells—laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury

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Regenerative Biomaterials 2023年第1期10卷 1481-1495页

作者： Yangyang Dai Liaobing Xin Sentao Hu Shiqian Xu Dong Huang Xiaoying Jin Jianmin Chen Rachel Wah Shan Chan Ernest Hung Yu Ng William Shu Biu Yeung lie ma Songying Zhang Assisted Reproduction Unit Department of Obstetrics and GynecologySir Run Run Shaw HospitalZhejiang University School of MedicineHangzhou 310016China Key Laboratory of Reproductive Dysfunction management of Zhejiang Province Hangzhou 310016China MOE Key Laboratory of macromolecular Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China Department of Obstetrics and Gynaecology School of Clinical MedicineLKS Faculty of MedicineThe University of Hong KongHong Kong SAR 999077China Shenzhen Key Laboratory of Fertility Regulation The University of Hong Kong Shenzhen HospitalShenzhen 518000China

Severe endometrium damage causes pathological conditions such as thin endometrium and intrauterine adhesion,resulting in uterine factor *** stem cell(MSC)therapy is a promising strategy in endometrial repair;yet,exogenous MSCs still raise concerns for safety and ethical *** adipose-derived mesenchymal stem cells(ADMSCs)residing in adipose tissue have high translational potentials due to their autologous *** harness the high translation potentials of ADMSC in clinical endometrium regeneration,here we constructed an ADMSCs composited porous scaffold(CS/ADMSC)and evaluated its effectiveness on endometrial regeneration in a rat endometrium-injury *** found that CS/ADMSC intrauterine implantation(i)promoted endometrial thickness and gland number,(ii)enhanced tissue angiogenesis,(iii)reduced fibrosis and(iv)restored *** ascertained the pro-proliferation,pro-angiogenesis,immunomodulating and anti-fibrotic effects of CS/ADMSC in vitro and revealed that the CS/ADMSC influenced extracellular matrix composition and organization by a transcriptomic *** results demonstrated the effectiveness of CS/ADMSC for endometrial regeneration and provided solid proof for our future clinical study.

关键词： adipose-derived mesenchymal stem cells collagen scaffold endometrium fibrosis regeneration