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Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture

Journal of Integrative Plant Biology 2018年第4期60卷 292-309页

作者： Sangseok lee lidiya l.sergeeva Dick Vreugdenhil laboratory of Plant Physiology Wageningen University&ResearchDroevendaalsesteeg 16708 PBWageningenThe Netherlands Gyeongsangbuk-Do Agricultural Research&Extension Services Centre 136 Gil-14Chilgokjungang-daeroDaeguSouth Korea

Studies on natural variation are an important tool to unravel the genetic basis of quantitative traits in plants. Despite the significant roles of phytohormones in plant development, including root architecture, hardly any studies have been done to investigate natural variation in endogenous hormone levels in plants. Therefore, in the present study a range of hormones were quantified in root extracts of thirteen Arabidopsis thaliana accessions using a ultra performance liquid chromatography triple quadrupole mass spectrometer. Root system architecture of the set of accessions was quantified, using a new parameter （mature root unit） for complex root systems, and correlated with the phytohormone data. Significant variations in phytohormone levels among the accessions were detected, but were remarkably small, namely less than three-fold difference between extremes. For cytokinins, relatively larger variations were found for ribosides and glucosides, as compared to the free bases. For root phenotyping, length-related traits--lateral root length and total root length--showed larger variations than lateral root number-related ones. For root architecture, antagonistic interactions between hormones, for example, indole-3-acetic acid to trans-zeatin were detected in correlation analysis. These findings provide conclusive evidence for the presence of natural variation in phytohormone levels in Arabidopsis roots, suggesting that quantitative genetic analyses are feasible.

关键词： Natural variation of hormone levels in Arabidopsis roots and correlations with complex root architecture

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Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in vitro

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Journal of Integrative Plant Biology 2015年第9期57卷 734-744页

作者： Oksana O.Kolachevskaya Valeriya V.Alekseeva lidiya I.sergeeva Elena B.Rukavtsova Irina A.Getman Dick Vreugdenhil Yaroslav I.Buryanov Georgy A.Romanov Signaling Systems laboratory Timiryazev Institute of Plant Physiology Russian Academy of Sciences Plant Biotechnology laboratory Branch of Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry Russian Academy of Sciences laboratory of Plant Physiology Wageningen University Department of Molecular Basis of Ontogenesis Belozersky Institute of Physico-Chemical Biology Moscow State University

Phytohormones, auxins in particular, play an important role in plant development and productivity. Earlier data showed positive impact of exogenous auxin on potato (Solanum tuberosum l.) tuberization. The aim of this study was to generate potato plants with increased auxin level predominantly in tubers. To this end, a pBinB33-tms1 vector was constructed harboring the Agrobacterium auxin biosynthesis gene tms1 fused to tuber-specific promoter of the class I patatin gene (B33-promoter) of potato. Among numerous independently generated B33:tms1 lines, those without visible differences from control were selected for detailed studies. In the majority of transgenic lines, tms1 gene transcription was detected, mostly in tubers rather than in shoots. Indoleacetic acid (IAA) content in tubers and the auxin tuber-to-shoot ratio were increased in tms1-expressing transformants. The organ-specific increase in auxin synthesis in B33:tms1-transformants accelerated and intensified the process of tuber formation, reduced the dose of carbohydrate supply required for in vitro tuberization, and decreased the photoperiodic dependence of tuber initiation. Overall, a positive correlation was observed between tms1 expression, IAA content in tubers, and stimulation of tuber formation. The revealed properties of B33:tms1 transformants imply an important role for auxin in potato tuberization and offer prospects to magnify potato productivity by a moderate organ-specific enhancement of auxin content.

关键词： Auxin gene expression Solanum tuberosum tms1 transgenic potato tuberization tuber yield

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Effects of Agrobacterial<i>rol</i>-Genes on the Thermodynamic and Structural Features of Starches Extracted from Potato Microtubers

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Food and Nutrition Sciences 2014年第3期5卷 250-257页

作者： luybov A. Wasserman Nina P. Aksenova Tatiyana N. Konstantinova lidiya I. sergeeva Svetlana A. Golyanovskaya Alexey V. Krivandin Georgy A. Romanov Emmanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia imiryazev Institute of Plant Physi- ology Russian Academy of Sciences Moscow Russia Timiryazev Institute of Plant Physi- ology Russian Academy of Sciences Moscow Russia Timiryazev Institute of Plant Physi- ology Russian Academy of Sciences Moscow Russia laboratory of Plant Physiology Wageningen University Wageningen The Netherlands.

Wild-type potato (Solanum tuberosum l.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorimetry (DSC). It was found that in starch from tubers of rolB plants the melting temperature of crystalline lamella was lower and their thickness was less than that in wild-type potato. In tubers of rolC plants starch differed from starch in wild-type plants by a higher melting temperature, reduced melting enthalpy, and a greater thickness of crystalline lamellae. The melting of starch from tubers of rolC plants proceeded as the melting of two independent crystalline structures with melting temperatures of 338.0°K and 342.8°K. Overall data show that starches of different structure can be obtained by using transgenic approach.

关键词： Solanum tuberosum Transgenic Potato Starch rol Genes Tubers Crystalline lamellae Melting Temperature

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Therapeutic molecules for multiple human diseases identified from pigeon pea(Cajanus cajan ***.)through GC–MS and molecular docking

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Food Science and Human Wellness 2017年第4期6卷 202-216页

作者： Deepu Mathew lidiya John P. Manila T.M. Divyasree P. Sandhya Rajan V.T.K. Finishing School in Biotechnology Centre for Plant Biotechnology and Molecular BiologyKerala Agricultural UniversityThrissurKerala 680656India

Molecular mechanism behind the therapeutic potential of pigeon pea over the human diseases such as rheumatoid arthritis,breast cancer,type II diabetes,malaria,measles and sickle cell disease were revealed through docking of GC–MS identified phyto-compound ligands with candidate disease *** the 242 ligands,three dimensional structures of 47 compounds had to be drawn using ChemSketch and the remaining structures were retrieved from PubChem and docked with the active sites of candidate *** molecules identified through docking were further subjected to ADMET analysis and promising drug candidates were identified for each *** paper presents a precise account of the chemoprofile of pigeon pea leaves,stems and seeds,interaction of these molecules with target proteins and suggests 26 highly potential molecules which are drug candidates for multiple human *** pea seeds are especially proven as invaluable source for therapeutic molecules.

关键词： Breast cancer Drug discovery Herbal medicine In silico Malaria Measles Phyto-compounds Rheumatoid arthritis Sickle cell disease Type II diabetes

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