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Slr0151 in Synechocystis sp. PCC 6803 is required for efficient repair of photosystem Ⅱ under high-light condition

Journal of Integrative Plant Biology 2014年第12期56卷 1136-1150页

作者： Haomeng Yang libing liao Tingting Bo Lei Zhao Xuwu Sun Xuefeng Lu Birgitta Norling Fang Huang Key Laboratory of Photobiology Institute of Botany the Chinese Academy of Sciences University of the Chinese Academy of Sciences Qingdao Institute of Bioenergy and Bioprocess Technology the Chinese Academy of Sciences School of Biological Sciences Nanyang Technological University

Cyanobacteria are ancient photosynthetic prokareyotes that have adapted successfully to adverse environments including high-light irradiation. Although it is known that the repair of photodamaged photosystem Ⅱ（PSⅡ） in the organisms is a highly regulated process, our knowledge of the molecular components that regulate each step of the process is *** have previously identified a hypothetical protein Slr0151 in the membrane fractions of cyanobacterium Synechocystis *** 6803. Here, we report that Slr0151 is involved in PSⅡ repair of the organism. We generated a mutant strain（Dslr0151）lacking the protein Slr0151 and analyzed its characteristics under normal and high-light conditions. Targeted deletion of slr0151 resulted in decreased PSⅡ activity in Synechocystis. Moreover,the mutant exhibited increased photoinhibition due to impairment of PSⅡ repair under high-light condition. Further analysis using in vivo radioactive labeling and 2-D blue native/sodium dodecylsulfate polyacrylamide gel electrophoresis indicated that the PSⅡ repair cycle was hindered at the levels of D1 synthesis and disassembly and/or assembly of PSⅡ in the mutant. Protein interaction assays demonstrated that Slr0151 interacts with D1 and CP43 proteins. Taken together,these results indicate that Slr0151 plays an important role in regulating PSⅡ repair in the organism under high-light stress condition.

关键词： Cyanobacteria membrane protein photosystem Ⅱ repair Slr0151 Synechocystis

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Chevrel phase: A review of its crystal structure and electrochemical properties

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Progress in Natural Science:Materials International 2023年第1期33卷 8-15页

作者： Meng Liu Guocheng Lv Tianming Liu Hao Liu Lingchang Kong Lintao Tian Wenxiu Rao Yuxin Li libing liao Juchen Guo Engineering Research Center of Ministry of Education for Geological Carbon Storage and Low Carbon Utilization of Resources Beijing Key Laboratory of Materials Utilization of Nonmetallic Minerals and Solid Wastes National Laboratory of Mineral Materials School of Materials Science and Technology China University of Geosciences(Beijing) School of Science China University of Geosciences Materials Science and Engineering Program University of California-Riverside Department of Chemical and Environmental Engineering University of California – Riverside

Chevrel phase compounds have attracted increasing attention as electrochemical energy storage materials and electro-catalysts. Benefiting from their unique crystal structure, Chevrel phase compounds can not only function as the host structures for reversible intercalation of a broad range of cations, but also exhibit high catalytic activity in electrochemical reduction reactions. Here we provide an overview of recent progress in the development of Chevrel phase materials including new understanding of structural features, synthetic methods, and electrochemical properties. A brief conclusion and perspectives on the future development of Chevrel phase materials are also provided.

关键词： Chevrel phase Electrochemical properties Energy storage Electro-catalysis

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HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley

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The Crop Journal 2023年第1期11卷 99-107页

作者： Deshui Yu Renchun Fan Ling Zhang Pengya Xue libing liao Meizhen Hu Yanjun Cheng Jine Li Ting Qi Shaojuan Jing Qiuyun Wang Arvind Bhatt Qian-Hua Shen Lushan Botanical Garden Chinese Academy of ScienceJiujiang 332900JiangxiChina State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesInnovation Academy for Seed DesignBeijing 100101China CAS Center for Excellence in Biotic Interactions University of Chinese Academy of SciencesBeijing 100049China

Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled *** barley,Hv WRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus,Blumeria graminis ***(Bgh).However,the molecular features of Hv WRKY2 in its DNA-binding and repressor functions,as well as its target genes,are *** show that the W-box binding of Hv WRKY2 requires an intact WRKY domain and an upstream sequence of~75 amino acids,and the Hv WRKY2 W-box binding activity is linked to its repressor function in disease *** immunoprecipitation(ChIP)-seq analysis identified HvCEBiP,a putative chitin receptor gene,as a target gene of Hv WRKY2 in overexpressing transgenic barley ***-qPCR and Electrophoretic Mobility Shift Assay(EMSA)verified the direct binding of Hv WRKY2 to a W-boxcontaining sequence in the HvCEBiP *** CEBiP positively regulates resistance against Bgh in *** findings suggest that Hv WRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern(PAMP)recognition receptor genes,suggesting that Hv CEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection.

关键词： HvWRKY2 Basal immunity Chromatin immunoprecipitation(ChIP) HvCEBiP

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Preparation and Characterization of Hydroxyiron-Montmorillonite Complexes

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Journal of Earth Science 2000年第3期19卷 178-180页

作者： liao libing Jia Fuli Faculty of Materials Science and Engineering, China University of Geossciences, Beijing 100083, China

Hydroxy Fe ions react slowly with montmorillonite by intercalation into their interlayer space and adsorption on their surface to form various hydroxyiron montmorillonite complexes. Hydroxyiron montmorillonite complexes are assumed to have the ability to adsorb anions such as H 2PO - 4, HPO 2- 4, CrO 2- 4, CrO - 2, H 2AsO - 4, HAsO 2- 4 , AsO 3- 4 and F -, which may come from weathered rocks or waste industrial water, and result in concentration of P, Cr, As and F in soil. In this paper, hydroxy Fe ions including Fe(OH) 2+ , Fe(OH) + 2 and Fe 2(OH) 2+ 2 are prepared through hydrolysis of iron in acid solutions with different pH values. Corresponding hydroxyiron montmorillonite complexes are obtained by intercalation and adsorption of these hydroxy Fe ions into the interlayer space and on the surface of montmorillonite. The obtained hydroxyiron montmorillonite complexes are characterized with XRD and DTA, showing that they have similar basal space and thermal stability with Fe montmorillonite.

关键词： hydroxy Fe ions hydroxyiron montmorillonite complex characterization.

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干燥方法对钛柱撑蒙脱石结构的影响

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硅酸盐学报 2005年第2期33卷 215-219,224页

作者：王丽娟廖立兵中国地质大学材料科学与工程学院北京100083

采用TiCl4水解法制备钛柱化剂,与蒙脱石离子交换后得到钛柱撑蒙脱石(Ti pillared montmorillonite,Ti PILM)。对制备的 TiPILM采用室温风干(air drying,AD)、40 ℃烘干(common drying,CD)、乙醇清洗后风干(ethanol extraction drying,EAD)、室温条件下真空干燥(vacuum drying,VD)等方法干燥,并在不同温度下热处理。用XRD,IR,DTA TG,N2等温吸附脱附等技术对 Ti PILM进行结构表征。结果表明:干燥方法对Ti PILM的结构及热稳定性有较大的影响。采用AD和EAD干燥方法可以得到2.7 nm左右大晶面间距Ti PILM,且AD干燥的样品在500 ℃下有很好的稳定性,比表面达285.7 m2/g,具有介孔孔径且孔径分布窄。而经CD和VD方法干燥焙烧后,蒙脱石层间钛柱坍塌,导致Ti PILM层状结构破坏,影响材料的热稳定性。

关键词：钛柱撑蒙脱石干燥热稳定

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环氧树脂/蛭石纳米复合材料的制备与表征

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硅酸盐学报 2006年第2期34卷 199-203页

作者：廖立兵黄振宇中国地质大学材料科学与工程学院北京100083

以环氧树脂为基体，二甲基苄胺为固化剂，经酸浸、加热和钠离子交换的结构修饰和有机改性的蛭石为增强剂，制备了环氧树脂／蛭石纳米复合材料。测试了环氧树脂／蛭石纳米复合材料的结构、形貌、力学和电学性能。结果表明：环氧树脂、二... 详细信息

以环氧树脂为基体，二甲基苄胺为固化剂，经酸浸、加热和钠离子交换的结构修饰和有机改性的蛭石为增强剂，制备了环氧树脂／蛭石纳米复合材料。测试了环氧树脂／蛭石纳米复合材料的结构、形貌、力学和电学性能。结果表明：环氧树脂、二甲基苄胺和蛭石的混合顺序，二甲基苄胺和蛭石的用量影响蛭石的剥离，进而影响环氧树脂／蛭石纳米复合材料的性能。在蛭石加入量为环氧树脂质量的1％～5％范围内，3％时环氧树脂／蛭石纳米复合材料的抗拉强度、弹性模量和弯曲强度最大，表面电阻最低，体电阻最高。

关键词：环氧树脂/蛭石纳米复合材料层剥离力学性能电学性能

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Preparation of Rubber Filler by Spent Activated Carbon

Preparation of Rubber Filler by Spent Activated Carbon

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中国材料大会2012

作者： Limei Wu Xiaoyu Wang libing liao Guocheng LV School of Material Sciences and Technology China University of Geosciences

ISBN: (纸本)9783037856062

In this paper, the spent Activated Carbon (AC) which came from caramel plant was regenerated by the method of microorganism and chemistry. The optimal regeneration method was the combination of microorganism regeneration and NaOH regeneration. The optimal regeneration condition was treated by microorganism in 60 min at temperature of 35 ℃ and in NaOH (dosage was 6%). The optimal ultrafine grinding time was 5 h at 1200 rpm by adding 5 ‰ Triethanolamine, the effect of ultrafine grinding was better and the minimal size distribution was D90 3.82μm, D50 1.62 μm. the study ofAC surface modification was explored while combining the similar characteristics of AC and Carbon Black in its structure and composition. The possibility of reusing the spent AC was studied and the mechanical performance of rubber with modified AC was 434% higher than that of unmodified AC. The modified AC was characterized by Scanning electron microscope (SEM).

关键词： Spent AC Rubber filler Surface modification.

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盱眙、明光、临泽坡缕石的八面体阳离子占位及对其性能的影响

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地质学报 2024年第3期98卷 876-888页

作者：廖立兵张培均刘昊中国地质大学(北京)材料科学与工程学院北京100083 中国地质大学(北京)数理学院北京100083

坡缕石是一种2:1型链层状结构含水富镁铝硅酸盐矿物,具有重要的资源和材料属性。不同成因、产状坡缕石八面体中类质同象替代有很大差别,而八面体类质同象替代对坡缕石性能的影响未见研究报道。本文以中国最具代表性的安徽明光、江苏盱... 详细信息

坡缕石是一种2:1型链层状结构含水富镁铝硅酸盐矿物,具有重要的资源和材料属性。不同成因、产状坡缕石八面体中类质同象替代有很大差别,而八面体类质同象替代对坡缕石性能的影响未见研究报道。本文以中国最具代表性的安徽明光、江苏盱眙、甘肃临泽坡缕石为研究对象,研究了三种坡缕石的Al、Mg、Fe含量及在八面体中的占位,并探讨了八面体的Al、Mg、Fe占位对坡缕石中水的种类和含量、表面电位和酸位以及对Pb^(2+)、Cd^(2+)吸附性能的影响。结果表明:明光坡缕石相对富镁,八面体主要被Mg^(2+)占据,具有三八面体特征;盱眙、临泽坡缕石相对富铝、铁,八面体主要被Al^(3+)占据,表现出更多的二八面体特征。盱眙坡缕石由于M1位置空位较多,结构水含量较低;坡缕石类质同象替代对Zeta电位影响不大;具有二八面体特征的盱眙、临泽坡缕石由于八面体中Al含量较高,酸位较多,催化性能较好。在重金属吸附实验中,明光坡缕石对Cd^(2+)的吸附效果最好,盱眙坡缕石对Pb^(2+)的吸附效果最好,临泽坡缕石吸附性能较差。本文可为坡缕石的改性和应用提供指导和参考。

关键词：坡缕石类质同象替代八面体占位吸附性能

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Mineralogical Study on A Graphite Tailing from Luobei, China

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矿物学报 2013年第S1期33卷 30-30页

作者： HAI Yun liao libing DU Gaoxiang L Guocheng School of Material Sciences and Technology China University of Geosciences

China produces the largest amount of graphite raw materials in the world and the largest amount of graphite tailings at the same time. At present, graphite tailings in China are up to 200 million tones, which not only occupy large area of land, cause environmental pollutions, the collapes of the tailings dam also threaten the life of local residents.

关键词： graphite tailings graphite mica process mineralogy

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New Organic Montmorillonite Preparation: Anion-Nonionic Surfactants Modified Montmorillonites

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矿物学报 2013年第S1期33卷 140-140页

作者： ZHANG Zepeng WAN Fang ZHANG Chaoqin liao libing School of Materials Sciences and Technology China University of Geosciences KaiPuQi Science and Technology Development Co. LTD

Montmorillonites (MMT) was modified with nonionic surfactant Trion X-100 (OP-10) and anionic surfactants sodium stearate (SSTA) and sodium dodecyl benzene sulfonate (SDBS) respectively. In this study, the effects of anionic surfactant types and MMT types on modification montmorillonite were investigated. XRD analysis results showed that SSTA/OP-10/MMT and SDBS/OP-10/MMT were successfully obtained. The basal spacing of SSTA/OP-10/MMT reached 5.07nm and the SSTA intercalation reaction was relatively stable. Different types of MMT (Xinjiang natural sodium montmorillonite(MMT-1), Jianping sodium montmorillonite (MMT-2) and artificial sodium montmorillonite(MMT-3)) were modified with SSTA/OP-10. XRD test results showed that MMT-1, MMT-2 and MMT-3 can be modified by SSTA/OP-10. Anion-nonionic organic montmorillonite (SSTA/OP-10/MMT-1, SSTA/OP-10/MMT-2 and SSTA/OP-10/MMT-3 were successfully obtained respectively. The higher expansion capacity was found to be beneficial to organic modification.

关键词： intercalation reaction sodium stearate sodium dodecyl benzene sulfonate Trion x-100

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