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Dynamic expression of P-glycoprotein in the CA1,CA3 and dentate gyrus of the rat hippocampus following lithium-pilocarpine-induced status epilepticus

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Neural Regeneration Research 2010年第24期5卷 1851-1857页

作者： Shuda Chen Jueqian Zhou Xiuling Liang Ziyi Chen libai yang Liemin Zhou Department of Neurology First Affiliated Hospital Sun Yat-sen University Guangzhou 510080 Guangdong Province China

Epileptic seizures induce overexpression of P-glycoprotein in the blood-brain barrier. However, it is unclear whether hippocampal neurons also overexpress P-glycoprotein following seizure. This study confirmed that the clinical manifestation, pathological characteristics and electroencephalography in the rat model of lithium-pilocarpine-induced mesial temporal lobe epilepsy were consistent with clinical reports of mesial temporal lobe epilepsy in *** staining demonstrated that P-glycoprotein positive staining was found in neurons in the pyramidal layer of the hippocampus. Westem blot assay and real-time polymerase chain reaction revealed that P-glycoprotein overexpression was exhibited in the CA1, CA3, and dentate gyrus of the hippocampus at 24 and 60 days following model induction, but no significant dffierence was detected in the same region at various time points. These results indicate that seizures led to overexpression of P-glycoprotein in neurons of the hippocampus, but no evidence was found for a positive association between P-glycoprotein expression and seizure frequency.

关键词： p-glycoprotein overexpression lithTum-pilocarpine status epilepticus pharmacoresistant epilepsy seizure frequency hippocampus neural regeneration

Anomalous expression of chloride transporters in the sclerosed hippocampus of mesial temporal lobe epilepsy patients

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Neural Regeneration Research 2013年第6期8卷 561-568页

作者： Xiaodong Cai libai yang Jueqian Zhou Dan Zhu Qiang Guo Ziyi Chen Shuda Chen Liemin Zhou Department of Neurology First Affiliated Hospital of Sun Yat-sen University Department of Neurosurgery Guangdong 999 Brain Hospital

The Na＋-K＋-CI- cotransporter 1 and K＋-CI- cotransporter 2 regulate the levels of intracellular chloride in hippocampal cells. Impaired chloride transport by these proteins is thought to be involved in the pathophysiological mechanisms of mesial temporal lobe epilepsy. Imbalance in the relative expression of these two proteins can lead to a collapse of CI- homeostasis, resulting in a loss of gamma-aminobutyric acid-ergic inhibition and even epileptiform discharges. In this study, we investigated the expression of Na＋-K＋-CI- cotransporter 1 and K＋-CI- cotransporter 2 in the sclerosed hippocampus of patients with mesial temporal lobe epilepsy, using western blot analysis and immunohistochemistry. Compared with the histologically normal hippocampus, the sclerosed hippocampus showed increased Na＋-K＋-Cl- cotransporter 1 expression and decreased K＋-CI- cotransporter 2 expression, especially in CA2 and the dentate gyrus. The change was more prominent for the Na＋-K＋-CI- cotransporter 1 than for the K＋-CI- cotransporter 2. These experimental findings indicate that the balance between intracellular and extracellular chloride may be disturbed in hippocampal sclerosis, contributing to the hyperexcitability underlying epileptic seizures. Changes in Na＋-K＋-CI-cotransporter 1 expression seems to be the main contributor. Our study may shed new light on possible therapies for patients with mesial temporal lobe epilepsy with hippocampal sclerosis.

关键词： neural regeneration brain injury mesial temporal lobe epilepsy hippocampal sclerosis sodium-potassium chloride cotransporter 1 potassium chloride cotransporter 2 gamma-aminobutyric acid chloride ion dentate gyrus CA2 region human grants-supported paperphotographs-containing paper neuroregeneration

Supramolecular Vesicles Based on Water-Soluble 2,6-Helic[6]arene:High Affinity Binding,Stimuli Responsiveness and Delivery of Doxorubicin to Cancer Cells

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Chinese Journal of Chemistry 2019年第6期37卷 575-580页

作者： Xuan yang Weipeng Mao Yamin Liu libai Li Da Ma Department of Chemistry Fudan University220 Handan RoadShanghai 200433China

Water-soluble 2,6-helic[6]arene was used to construct supramolecular vesicles via host-guest interaction. Water-soluble 2,6-helic[6]arene was discovered to be high affinity host for suitable biomarkers. Supramolecular vesicles were responsive to multiple stimuli types, including temperature, pH, Ca^2+, CO2 bubbling and biomarker displacement. Supramolecular vesicles were used to load and deliver anti-tumor drug doxorubicin to HeLa cells in vitro.

关键词： host-guest systems nanostructures molecular recognitions self-assembly supramolecular vesicles

SPAK参与药物抗性癫痫小鼠海马神经元GABA信号的功能重塑

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中西医结合心脑血管病杂志 2017年第23期15卷 2980-2986页

作者：杨丽白蔡晓冬周列民牛争平李新毅山西大医院/山西医学科学院太原030012 中山大学附属六院中山大学附属第一医院山西医科大学第一医院

目的研究氯化锂-匹罗卡品诱导的癫痫(PISE)小鼠海马神经元生理生化的变化,探讨药物抗性癫痫的γ-氨基丁酸(GABA)信号功能的重塑机制。方法将4周~6周健康雄性Balb/c小鼠制作成PISE癫痫模型,动物随机分为3组:癫痫模型组、假模型组和空白组... 详细信息

目的研究氯化锂-匹罗卡品诱导的癫痫(PISE)小鼠海马神经元生理生化的变化,探讨药物抗性癫痫的γ-氨基丁酸(GABA)信号功能的重塑机制。方法将4周~6周健康雄性Balb/c小鼠制作成PISE癫痫模型,动物随机分为3组:癫痫模型组、假模型组和空白组,造模后观察1 d(静息期)、14 d(亚急性期)、45 d(慢性自发癫痫发作期)三个时间点,每组每个时间点各6只动物。采用免疫双标观察201S1相关的氨酸/丙氨酸激酶/Na+-K+-2Cl-共转运体1(NKCC1)、SPAK/K+–2Cl-共转运体2(KCC2)在海马组织共表达情况。Western Blot检测各组小鼠海马组织SPAK蛋白表达的差异。用Cl-荧光探针MQAE检测各组神经元Cl-分布情况。免疫共沉淀法检测SPAK与NKCC1、KCC2是否存在相互作用。结果 SPAK/NKCC1、SPAK/KCC2在小鼠海马组织共表达。与空白对照组及假模型组比较,模型组在PISE后1 d、14 d、45 d海马组织SPAK表达均增高,差异有统计学意义(P<0.05);模型组3个时间点比较,14 d高于1 d、45 d,与后者比较差异有统计学意义(P<0.05)。模型组小鼠海马[Cl-]i下降(P<0.05)。慢性自发性癫痫小鼠SPAK与NKCC1、KCC2分别存在相互作用。结论小鼠海马神经元SPAK通过与NKCC1、KCC2相互作用调控[Cl-],参与药物抗性癫痫GABA信号功能的重塑。

关键词：药物抗性癫痫 γ氨基丁酸 SPAK

A New Pseudospectral Method for Calculations of Hydrogen Atom in Arbitrary External Fields

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Communications in Theoretical Physics 2002年第1期37卷 15-20页

作者： QIAOHao－Xue libai－Wen WuhanInstituteofPhysicsandMathematics TheChineseAcademyofSciencesWuhan430071China

A new pseudospectral method was introduced to calculate wavefunctions and energy levels of hydrogen atom in arbitrary potential. Some results of hydrogen atom in uniform magnetic fields were presented, high accuracy of results was obtained with simple calculations, and our calculations show very fast convergence. It suggests a new methodfor calculations of hydrogen atom in external fields.

关键词： pseudospectral magnetic fields hydrogen

Spectral Properties of Endohedrally Confined Hydrogen Atom and Hydrogen—Like Ions Obtained by Using B—Spline Basis Set

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Communications in Theoretical Physics 2002年第4期37卷 475-478页

作者： QIAOHao－Xue libai－Wen WuhanInstituteofPhysicsandMethematics theChineseAcademyofSciencesWuhan430071China

A B-spline method has been used to calculate the electron structure of cndohedrally confined hydrogenlike *** boundary conditions were conveniently satisfied with such the *** evolution of the energy spectrum,as function of the depth of the confining well,exhibits a "mirror collapse".Ions with higher ionicity have more "collapse lines",the energies change more sharply at "collapse points",and the oscillator strengths change more violently with the depth of the confining well.

关键词：氢原子禁闭氢原子谱类氢离子 B-Spline

“伏九”穴位敷贴治疗常年性变应性鼻炎41例总结

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湖南中医杂志 2012年第3期28卷 2-4页

作者：黄香红李金香章薇娄必丹李晓屏刘兴平李武杨礼白杨淑荃湖南中医药大学 2004级7年制湖南长沙410007 湖南中医药大学第一附属医院湖南长沙410007

目的:观察"伏九"穴位敷贴治疗常年性变应性鼻炎的临床疗效。方法:将125例患者随机分为"伏九"组41例、三伏组44例和三九组40例,"伏九"组在三伏、三九进行穴位敷贴,三伏组在三伏进行穴位敷贴,三九组在三九进行穴位敷贴。结果:"伏九"组总... 详细信息

目的:观察"伏九"穴位敷贴治疗常年性变应性鼻炎的临床疗效。方法:将125例患者随机分为"伏九"组41例、三伏组44例和三九组40例,"伏九"组在三伏、三九进行穴位敷贴,三伏组在三伏进行穴位敷贴,三九组在三九进行穴位敷贴。结果:"伏九"组总有效率为97.5%,三伏组为84.09%,三九组为72.50%;"伏九"穴位敷贴优于单独三伏和三九穴位敷贴,三伏穴位敷贴疗效优于三九穴位敷贴(P<0.05);且皮肤出现发泡的患者疗效优于皮肤未发泡的患者(P<0.05)。结论:"伏九"敷贴治疗变应性鼻炎有较好疗效。

关键词：变应性鼻炎外治法 “伏九”穴位敷贴

APPLICATION OF THREE-DIMENSIONAL VISULIZATION IN SIMULATION OF GEOGRAPHICAL LANDSCAPE EVOLVEMENT

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Chinese Geographical Science 2005年第2期15卷 168-172页

作者： PANJun XINGLi-xin libai-shou MENGTao CollegeofGeo-explorationandInformationTechnology JilinUniversityChangchun130026P.R.China

The value of application of three-dimensional visualization and animation technique in dynamic simulation of geographical landscape formation is analyzed and, in particular, how to simulate the formation and evolvement of geographical landscape in temporal dimension is discussed thoroughly. Based on various modeling tools in 3DS MAX and original DEM data of the study area acquired from topographic map, real three-dimensional terrain model is generated by using the method of three-dimensional mesh approximation through DEM interpolating and surface modeling, which leads to the realization of the dynamic visualization and simulation of volcanic landscape, formation and evolvement. Furthermore, the dynamic three-dimensional visual virtual scenery of the formation and evolvement of the volcano in the Changbai Mountains of Jilin, China, is constructed. The applicability, potential, and corresponding technique of using 3DS MAX to dynamically simulate the formation of geographical landscape are expatiated.

关键词： three-dimensional visualization geographical landscape volcano ChangbaiMountains

Calculations of Electron Structure of Endohedrally Confined Helium Atom with B—Spline Type Functions

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Communications in Theoretical Physics 2002年第2期37卷 221-224页

作者： QIAOHao－Xue libai－Wen 等 LaboratoryofMagneticResonanceandAtomicandMolecularPhysics WuhanInstituteofPhysicsandMathematicsTheChineseAcademyofSciencesWuhan430071China CCAST（WoldLaboratory） P.O.Box8730

The B-spline basis set method is used to study the properties of helium confined endohedrally at thegeometrical centre of a fullerene. The boundary conditions of the wavefunctions can be simply satisfied with thismethod. From our results, the phenomenon of 'mirror collapse' is found in the case of confining helium. The interestingbehaviors of confining helium are also discussed.

关键词： B-spline fullerene mirror collapse