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Journal of Analysis and Testing 2023年第2期7卷 128-135页

作者： Ni Zeng Xinyi Wang Yiyang Dong Yan Yang Yingai Yin lianhui zhao Xu Wang College of Life Science and Technology Beijing University of Chemical TechnologyBeijing 100029China

Okadaic acid(OA),a small molecule substance derived from shellfish,is one of the most widely distributed marine toxins with acute symptoms of vomiting and diarrhea after accidental ingestion.For this,there is an urgently need for sensitive and reliable methods to detect OA in real shellfish samples.In this study,a simple aptasensor based on screen-printed carbon electrode(SPCE)with modification of chitosan(CS)and gold nanoparticles(Au NPs)was designed for electrochemical determination of OA,and the electrode surface was modified with Au NPs by potential-sweeping electrodeposition,which greatly improved the electrochemical response.The entire detection and characterization process were carried out by cyclic voltammetry(CV)with a linear correlation in the range of 0.01-100 ng/m L and a limit of detection(LOD)of 6.7 pg/m L.Furthermore,recovery rates of 92.3-116%were obtained demonstrating excellent accuracy through the recovery trial of mussel and scallop samples.

关键词： Okadaic acid Aptasensor Screen-printed carbon electrode Chitosan Gold nanoparticles Cyclic voltammetry

Cholesterol accumulation on dendritic cells reverses chronic hepatitis B virus infection-induced dysfunction

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Cellular & Molecular Immunology 2022年第12期19卷 1347-1360页

作者： Huajun zhao Yating Yu Yucan Wang lianhui zhao Ailu Yang Yifei Hu zhaoyi Pan Zixuan Wang Jiarui Yang Qiuju Han Zhigang Tian Jian Zhang Institute of Immunopharmaceutical Sciences School of Pharmaceutical SciencesShandong UniversityJinanShandong250012China Department of Gastroenterology Shandong Provincial HospitalCheeloo College of MedicineShandong UniversityJinanShandong250012China School of Life Sciences University of Science and Technology of ChinaHefei230000China

Chronic hepatitis B(CHB)infection remains a serious public health problem worldwide;however,the relationship between cholesterol levels and CHB remains unclear.We isolated peripheral blood mononuclear cells from healthy blood donors and CHB patients to analyze free cholesterol levels,lipid raft formation,and cholesterol metabolism-related pathways.Hepatitis B virus(HBV)-carrier mice were generated and used to confirm changes in cholesterol metabolism and cell-surface lipid raft formation in dendritic cells(DCs)in the context of CHB.Additionally,HBV-carrier mice were immunized with a recombinant HBV vaccine(rHBVvac)combined with lipophilic statins and evaluated for vaccine efficacy against HBV.Serum samples were analyzed for HBsAg,anti-HBs,and alanine aminotransferase levels,and liver samples were evaluated for HBV DNA and RNA and HBcAg.CHB reduced free cholesterol levels and suppressed lipid raft formation on DCs in patients with CHB and HBV-carrier mice,whereas administration of lipophilic statins promoted free cholesterol accumulation and restored lipid rafts on DCs accompanied by an enhanced antigen-presentation ability in vitro and in vivo.Cholesterol accumulation on DCs improved the rHBVvac-mediated elimination of serum HBV DNA and intrahepatic HBV DNA,HBV RNA,and HBcAg and promoted the rHBVvac-mediated generation and polyfunctionality of HBV-specific CD11a^(hi) CD8α^(lo) cells,induction of the development of memory responses against HBV reinfection,and seroconversion from HBsAg to anti-HBs.The results demonstrated the important role of cholesterol levels in DC dysfunction during CHB,suggesting that strategies to increase cholesterol accumulation on DCs might enhance therapeutic vaccine efficacy against HBV and support development toward clinical CHB treatment.

关键词： Chronic hepatitis B Dendritic cells Cholesterol metabolism Therapeutic vaccine

三湖国家级自然保护区不同林型食腐甲虫群落结构及多样性

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东北林业大学学报 2024年第7期52卷 111-118页

作者：王佳生孟庆繁刘生冬郑宇婷赵红蕊苏连辉吉林省长白山区昆虫生物多样性与生态系统功能重点实验室(北华大学) 吉林省吉林市132013 吉林省林业勘察设计研究院

为了解松花江三湖国家级自然保护区不同林型中食腐甲虫群落结构及多样性特征,通过悬挂高、低拦截式挡板诱捕器对食腐甲虫进行连续采集,利用单因素方差分析判断不同林型甲虫群落结构及α多样性指数差异,利用皮尔森相关系数及冗余分析判... 详细信息

为了解松花江三湖国家级自然保护区不同林型中食腐甲虫群落结构及多样性特征,通过悬挂高、低拦截式挡板诱捕器对食腐甲虫进行连续采集,利用单因素方差分析判断不同林型甲虫群落结构及α多样性指数差异,利用皮尔森相关系数及冗余分析判断不同α多样性指数间相关性及对林分因子倾向性,利用非度量多维尺度分析、置换多元方差分析、相似性百分比及杰卡德系数判断不同林型间甲虫β多样性。结果表明:共采集到食腐甲虫昆虫6027头,隶属于47科208属311种。其中,单属单种的科为15种,3种林型食腐甲虫共有科为31种,共有物种为64种。对比同一林型中不同食性及不同林型中同一食性甲虫个体数与物种数时,仅有粪食性甲虫个体数在不同林型间无显著差异。不同林型优势科差异显著,其中,鳃金龟科个体数与物种数差异极显著,叩甲科个体数与物种数差异显著,埋葬甲科个体数差异显著,物种数差异极显著;同一林型不同优势科个体数与物种数差异均为极显著。不同林型优势种个体数差异明显,其中,褐绒绢金龟、阔胫玛绢金龟和棕籽绒绢金龟差异极显著,黑斑锥胸叩甲差异显著,Dalopius puerilis无显著差异;同一林型不同优势种个体数差异均为极显著。α多样性在不同林型具体表现为个体数、物种数、香农指数、辛普森指数与皮洛指数差异极显著,玛格列夫指数、Chao1指数与ACE指数差异显著;其中,玛格列夫指数、Chao1指数、ACE指数与郁闭度和平均胸径呈较强正相关性,物种数与枯落物厚度呈极强正相关性,个体数与植被盖度呈较强正相关性,香农指数、辛普森指数与皮洛指数与植被盖度呈负相关性。β多样性显示,不同林型间群落结构差异显著,且褐绒绢金龟、棕籽绒绢金龟和阔胫码绢金龟对群落差异贡献较大,各林型间相似性均互为中等不相似。

关键词：三湖自然保护区林型食腐甲虫群落结构多样性

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Investigation on microstructures and mechanical properties of Mg-6Zn-0.5Ce-xMn(x=0 and 1)wrought magnesium alloys

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Journal of Magnesium and Alloys 2022年第4期10卷 993-1003页

作者： Caihong Hou Hongshuai Cao Fugang Qi Qing Wang lianhui Li Nie zhao Dingfei Zhang Xiaoping Ouyang School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105PR China China Railway Eryuan Engineering Group Co.Ltd. Chengdu 610000PR China College of Materials Science and Engineering Chongqing UniversityChongqing 400045PR China

The microstructure evolution and mechanical properties of Mg–6Zn–0.5Ce–xMn(x=0 and 1 wt.%)wrought magnesium alloys were researched,and the morphologies and role of Mn element in the experimental alloys were analyzed.The research shows that all of Mn elements form theα-Mn pure phases,which do not participate in the formation of other phases,such as theτ-phases.The mechanical properties of Mn-containing alloys in as-extruded and aged states are superior to Mn-free alloys.During the hot extrusion process,the dispersed fineα-Mn particle phase hinders the migration of grain boundaries and inhibits dynamic recrystallization,which mainly takes effect of grain refining and dispersion hardening.During the aging treatments,the dispersed fineα-Mn particle phase not only hinders the growth of the solution-treated grains,but also becomes the nucleation cores ofβ1 rod-like precipitate phase,which is conducive to increasing the nucleation rate of the precipitate phase.For the aged alloy,the Mn addition mainly takes effect of grain refining and promoting aging strengthening.

关键词： Mg–6Zn–0.5Ce alloy Microstructure evolution Mechanical property Mn element Aging precipitation

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弯曲乳杆菌与清酒乳杆菌的比较基因组学分析

食品科学 2024年第2期45卷 48-56页

作者：何苗赵雨晴李居行葛佳琪陈盼婷应欣张连慧王昌禄李贞景郭庆彬刘欢欢天津科技大学食品科学与工程学院天津300457 中粮营养健康研究院有限公司北京102209

本研究对19株弯曲乳杆菌(Latilactobacillus curvatus)和40株清酒乳杆菌(L.sakei)的基因组进行了比较分析。平均核苷酸同一性及全基因组共线性分析表明,L.curvatus和L.sakei基因组间的核酸序列同源性较弱,可作为区分两个物种的指标。对... 详细信息

本研究对19株弯曲乳杆菌(Latilactobacillus curvatus)和40株清酒乳杆菌(L.sakei)的基因组进行了比较分析。平均核苷酸同一性及全基因组共线性分析表明,L.curvatus和L.sakei基因组间的核酸序列同源性较弱,可作为区分两个物种的指标。对两个物种分别构建泛基因组,并对泛基因组中的核心基因功能进行注释。结果显示,L.curvatus和L.sakei各自的核心基因组主要涉及菌株的基础代谢。对菌株个体基因组的比对分析发现:1)L.curvatus与L.sakei均含有广泛的糖苷水解酶类编码基因,在分解和代谢膳食纤维类多糖、乳糖利用以及木质纤维素中具有丰富的基因资源;2)3个菌株含抗生素耐药性基因,且来源于基因横向转移;3)L.sakei特有的精氨酸脱亚胺酶途径、L.curvatus中的丝氨酸脱水酶途径及鸟嘌呤脱氨酶途径,以及个别菌株中谷氨酸脱羧酶的发掘,揭示了这两类菌不同的抗酸机制;4)冷应激蛋白编码基因的发现也赋予了这两类菌良好的冷加工特性。此外,部分L.sakei基因组中含有编码lactocin S及凝结素相关的基因簇。总之,本研究通过对L.curvatus及L.sakei进行基因组水平的比较和分析,提供了两个物种之间的分类标准以及菌株个体上的差异信息,为这两种乳酸菌的生理生化分子遗传机制研究以及工业应用奠定了基础。

关键词：弯曲乳杆菌清酒乳杆菌基因组学泛基因组碳水化合物活性酶抗生素耐药性

从国内专家共识推荐意见更新探讨经颈静脉肝内门体分流术的临床应用进展

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中华消化病与影像杂志（电子版） 2024年第3期14卷 193-196页

作者：戚泽雪赵连晖王广川张春清山东大学山东省立医院济南250021 山东第一医科大学附属省立医院消化内科济南250021 山东大学齐鲁医院感染性疾病科济南250021

经颈静脉肝内门体分流术是治疗肝硬化门静脉高压及其并发症的关键治疗策略之一。本文主要结合国内四版相关指南和专家共识的推荐意见更新趋势,比较并总结经颈静脉肝内门体分流术在国内的临床应用进展。

关键词：门静脉高压经颈静脉肝内门体分流术肝硬化临床应用进展

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虚拟仿真技术在医学实验教学中的应用

电子技术（上海） 2024年第2期53卷 200-201页

作者：周明君颜南赵莲辉林洪竹刘洪沈阳开放大学辽宁110003 沈阳医学院辽宁110034

阐述虚拟仿真技术实验教学中的沉浸性、探索性和交互性特点。分析实验教学中的计算机虚拟仿真技术应用,展现突发传染病的真实情景,促进各种教学模式融合。

关键词：虚拟仿真实验教学交互性

Cell division factor ZapE regulates Pseudomonas aeruginosa biofilm formation by impacting the pqs quorum sensing system

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mLife 2023年第1期2卷 28-42页

作者： Xi Liu Minlu Jia Jing Wang Hang Cheng zhao Cai zhaoxiao Yu Yang Liu Luyan ZMa lianhui Zhang Yingdan Zhang Liang Yang State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijingChina Key University Laboratory of Metabolism and Health of Guangdong School of MedicineSouthern University of Science and TechnologyShenzhenChina Guangdong Province Key Laboratory of Microbial Signals and Disease Control Integrative Microbiology Research CenterSouth China Agricultural UniversityGuangzhouChina Medical Research Center Southern University of Science and Technology HospitalShenzhenChina Shenzhen Third People's Hospital National Clinical Research Center for Infectious DiseaseThe Second Affiliated Hospital of Southern University of Science and TechnologyShenzhenChina

Pseudomonas aeruginosa is one of the leading nosocomial pathogens that causes both severe acute and chronic infections.The strong capacity of P.aeruginosa to form biofilms can dramatically increase its antibiotic resistance and lead to treatment failure.The biofilm resident bacterial cells display distinct gene expression profiles and phenotypes compared to their free-living counterparts.Elucidating the genetic determinants of biofilm formation is crucial for the development of antibiofilm drugs.In this study,a highthroughput transposon-insertion site sequencing(Tn-seq)approach was employed to identify novel P.aeruginosa biofilm genetic determinants.When analyzing the novel biofilm regulatory genes,we found that the cell division factor ZapE(PA4438)controls the P.aeruginosa pqs quorum sensing system.The ΔzapE mutant lost fitness against the wild-type PAO1 strain in biofilms and its production of 2-heptyl-3-hydroxy-4(1H)-quinolone(PQS)had been reduced.Further biochemical analysis showed that ZapE interacts with PqsH,which encodes the synthase that converts 2-heptyl-4-quinolone(HHQ)to PQS.In addition,site-directed mutagenesis of the ATPase active site of ZapE(K72A)abolished the positive regulation of ZapE on PQS signaling.As ZapE is highly conserved among the Pseudomonas group,our study suggests that it is a potential drug target for the control of Pseudomonas infections.

关键词： biofilm Pseudomonas aeruginosa Pseudomonas quinolone signal transposon‐insertion site sequencing(Tn‐seq) ZapE

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盘形滚刀三维刀间距数值模拟与分析

矿山机械 2024年第6期52卷 9-16页

作者：张照煌兰越韩佳霖许成发苏翠霞贾连辉赵宏华北电力大学能源动力与机械工程学院北京102206 中国铁建重工集团股份有限公司湖南长沙410100 北方重工集团有限公司辽宁沈阳110141 中铁工程装备集团有限公司河南郑州450016

刀间距是全断面岩石掘进机(TBM)盘形滚刀布置的重要参数,对TBM破岩作业效能影响较大,因此,一直是TBM刀盘上盘形滚刀布置研究的难点。但对刀间距的研究,国内外通常采用盘形滚刀线性滚压破碎岩石的试验方法,这与盘形滚刀实际圆形滚压破碎... 详细信息

刀间距是全断面岩石掘进机(TBM)盘形滚刀布置的重要参数,对TBM破岩作业效能影响较大,因此,一直是TBM刀盘上盘形滚刀布置研究的难点。但对刀间距的研究,国内外通常采用盘形滚刀线性滚压破碎岩石的试验方法,这与盘形滚刀实际圆形滚压破碎岩石的作业机制相比,有较大局限性。因此采用ABAQUS软件,建立了盘形滚刀圆形滚压破碎岩石的数字模型,并进行了数字模拟仿真,深入研究了盘形滚刀安装半径、贯入度与最优刀间距之间的关系,建立了盘形滚刀在刀盘上安装布置的三维刀间距理论。工程模拟应用发现,采用三维刀间距理论布置盘形滚刀的TBM,其刀盘推力、转矩和破岩比能分别降低7.4%、9.5%和10.2%。

关键词： TBM 盘形滚刀最优刀间距

Comparative characterization of antibody responses induced by Ad5-vectored spike proteins of emerging SARS-CoV-2 VOCs

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Signal Transduction and Targeted Therapy 2022年第8期7卷 3126-3138页

作者： Busen Wang Jinghan Xu Shipo Wu Zhe Zhang Zhenghao zhao Jun Zhang Ling Fu Xiaodong Zai Yudong Wang Guanying Zhang ZhengShan Chen Yi Chen Hancong Sun Xiaohong Song Jinlong Zhang lianhui Zhu Lihua Hou Wei Chen Beijing Institute of Biotechnology No.20 Dongdajie StreetFengtai District100071 BeijingChina

Highly divergent SARS-CoV-2 variants have continuously emerged and spread around the world,and updated vaccines and innovative vaccination strategies are urgently needed to address the global SARS-COV2 pandemic.Here,we established a series of Ad5-vectored SARS-CoV-2 variant vaccines encoding multiple spike proteins derived from the Alpha,Beta,Gamma,Epsilon,Kappa,Delta and Omicron lineages and analyzed the antibody immune responses induced by single-dose and prime-boost vaccination strategies against emerging SARS-CoV-2 variants of concern(VOCs).Single-dose vaccination with SARS-CoV-2 variant vaccines tended to elicit the optimal self-matched neutralizing effects,and Ad5-B.1.351 produced more broad-spectrum cross-neutralizing antibodies against diverse variants.In contrast,prime-boost vaccination further strengthened and broadened the neutralizing antibody responses against highly divergent SARS-CoV-2 variants.The heterologous administration of Ad5-B.1.617.2 and Ad5-B.1.429 to Ad5-WT-primed mice resulted in superior antibody responses against most VOCs.In particular,the Omicron spike could only stimulate self-matched neutralizing antibodies with infrequent cross-reactivities to other variants used in single-dose vaccination strategies;moreover,with prime-boost regimens,this vaccine elicited an optimal specific neutralizing antibody response to Omicron,and prompted cross-antibody responses against other VOCs that were very similar to those obtained with Ad5-WT booster.Overall,this study delineated the unique characteristics of antibody responses to the SARS-CoV-2 VOC spikes with the single-dose or prime-boost vaccination strategies and provided insight into the vaccine development of next SARS-CoV-2 VOCs.

关键词： neutral optimal matched