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Polymorphisms of potential drug resistant molecular markers in Plasmodium vivax from China-Myanmar border during 2008-2017

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Infectious Diseases of Poverty 2022年第2期11卷 93-94页

作者： Zhensheng Wang Chunyan Wei Yunchun Pan Zhihua Wang Xin Ji Qianqian Chen lianhui zhang Zenglei Wang Heng Wang Department of Microbiology and Parasitology Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical College5#Dong Dan San TiaoBeijing 100005People’s Republic of China NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical College9#Dong Dan San TiaoBeijingPeople’s Republic of China 不详

Background:Plasmodium vivax remains the predominant species at the China–Myanmar border,imposing a major challenge to the recent gains in regional malaria *** closely supervise the emerging of drug resistance in this area,we surveyed the variations in genes potentially correlated with drug resistance in *** parasite and the possible drug selection with ***:A total of 235 *** samples were collected from patients sufering uncomplicated malaria at Yingjiang,Tengchong,and Longling counties,and Nabang port in China,Yunnan province,and Laiza sub-township in Myanmar,from 2008 to *** potential drug resistance genes were amplifed utilizing nested-PCR and analyzed,including pvdhfr,pvdhps,pvmdr1,pvcrt-o,and *** Pearson’s Chi-squared test or Fisher’s exact test were applied to determine the statistical frequency diferences of mutations between categorical ***:The pvdhfr F57I/L,S58R,T61M and S117T/N presented in 40.6%,56.7%,40.1%,and 56.0% of the sequenced *** isolates,and these mutations signifcantly decreased with *** haplotype formed by these quadruple mutations predominated in Yingjiang,Tengchong,Longling and *** a mutation H99S/R(56.6%)dominated in Laiza and increased with *** pvdhps,the A383G prevailed in 69.2% of the samples,which remained the most prevalent ***,a signifcant decrease of its occurrence was also noticed over the *** S382A/C and A553G existed in 8.4% and 30.8% of the isolates,*** pvmdr1,the mutation Y976F occurred at a low frequency in 5/232(2.2%),while T958M was fxed and F1076L was approaching fxed(72.4%).The K10 insertion was detected at an occurrence of 33.2% in pvcrt-o,whereas there was no signifcant diference among the sites or over the *** mutation was identifed in ***:Mutations related with resistance to antifolate drugs are prevalent in this area,while their frequencies decrease signifcantly with time,suggestive of increased suscep

关键词： Plasmodium vivax Antimalarial drug Drug resistance Molecular marker China-Myanmar border

A heme-activatable probe and its application in the high-throughput screening of Plasmodium falciparum ring-stage inhibitors

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Signal Transduction and Targeted Therapy 2022年第6期7卷 1882-1884页

作者： Sheng Liu Chunyan Wei Tian Liu Shuang-Gang Ma Chen Chen Hao Lin lianhui zhang Heng Wang Chong-Jing zhang Shi-Shan Yu State Key Laboratory of Bioactive Substance and Function of Natural Medicines and Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Department of Microbiology and Parasitology Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical College5#Dong Dan San TiaoBeijing 100005China

Dear Editor,The resistance of Plasmodium falciparum to artemisinin derivatives(ARTs)in Asian,African and South American countries is a pressing global *** is an important biomarker of malaria parasites and is closely related to the mode of action of ARTs,but no heme-activatable fluorescent probe has been developed to study the biology of heme in malaria parasites in a light-up ***,we rationally developed probes from the unique reaction between heme and Yingzhaosu A(YZSA),then we unprecedentedly applied the probe to identify ring-stage inhibitors of ***.

关键词： falciparum heme Plasmodium

Cell division factor ZapE regulates Pseudomonas aeruginosa biofilm formation by impacting the pqs quorum sensing system

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mLife 2023年第1期2卷 28-42页

作者： Xi Liu Minlu Jia Jing Wang Hang Cheng Zhao Cai Zhaoxiao Yu Yang Liu Luyan ZMa lianhui zhang Yingdan zhang Liang Yang State Key Laboratory of Microbial Resources Institute of MicrobiologyChinese Academy of SciencesBeijingChina Key University Laboratory of Metabolism and Health of Guangdong School of MedicineSouthern University of Science and TechnologyShenzhenChina Guangdong Province Key Laboratory of Microbial Signals and Disease Control Integrative Microbiology Research CenterSouth China Agricultural UniversityGuangzhouChina Medical Research Center Southern University of Science and Technology HospitalShenzhenChina Shenzhen Third People's Hospital National Clinical Research Center for Infectious DiseaseThe Second Affiliated Hospital of Southern University of Science and TechnologyShenzhenChina

Pseudomonas aeruginosa is one of the leading nosocomial pathogens that causes both severe acute and chronic *** strong capacity of *** to form biofilms can dramatically increase its antibiotic resistance and lead to treatment *** biofilm resident bacterial cells display distinct gene expression profiles and phenotypes compared to their free-living *** the genetic determinants of biofilm formation is crucial for the development of antibiofilm *** this study,a highthroughput transposon-insertion site sequencing(Tn-seq)approach was employed to identify novel *** biofilm genetic *** analyzing the novel biofilm regulatory genes,we found that the cell division factor ZapE(PA4438)controls the *** pqs quorum sensing *** ΔzapE mutant lost fitness against the wild-type PAO1 strain in biofilms and its production of 2-heptyl-3-hydroxy-4(1H)-quinolone(PQS)had been *** biochemical analysis showed that ZapE interacts with PqsH,which encodes the synthase that converts 2-heptyl-4-quinolone(HHQ)to *** addition,site-directed mutagenesis of the ATPase active site of ZapE(K72A)abolished the positive regulation of ZapE on PQS *** ZapE is highly conserved among the Pseudomonas group,our study suggests that it is a potential drug target for the control of Pseudomonas infections.

关键词： biofilm Pseudomonas aeruginosa Pseudomonas quinolone signal transposon‐insertion site sequencing(Tn‐seq) ZapE

A convenient gene deletion toolbox for genetic analysis in Ralstonia solanacearum

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A convenient gene deletion toolbox for genetic analysis in R...

Field Control Effect of Biocontrol Bacteria Pseudomonas guariconensis Strain ST4 on Sugarcane Smut

中国植物病理学会2019年学术年会

作者： Yan Jinli zhang lianhui Guangdong Province Key Laboratory of MicrobialSignals and Disease control Integrative Microbiology Research CentreSouth China Agricultural University

Ralstonia solanacearum,a soilborne bacterial pathogen,is the causal agent of bacterial wilt and infects over 200 plant species in 50 *** pathogen is lethal to many solanaceous species,including eggplant,tobacco and *** pathogen is notorious for its genetic variations,and the regulatory mechanism that governs the pathogenicity of *** is not well—*** this study,we set to develop a convenient and reliable delivery method for stable and directed deletion of gene or DNA sequence in the genome of *** method is modified from the suicide vector pRC,which was developed previously for gene knock—in at a permissive site downstream of glmS in the genome of *** strain GMI1000 through homologous *** modified gene deletion system consists of a fusion DNA fragment containing the upstream and downstream region of a target gene and a gentamycin resistance gene as a selection marker,as well as a growth medium which primes *** to a state prone to receiving DNA fragments for *** feasibility of this gene deletion system was tested by targeting a regulatory gene in *** EP1,which is a virulent strain isolated from the diseased eggplant in Guangdong Province of *** results showed that the resultant mutants displayed much reduced EPS *** of such a convenient gene deletion system would facilitate further investigations of the pathogenic mechanisms of this important bacterial pathogen.

关键词： Ralstonia solanacearum gene deletion toolbox genetic analysis

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Field Control Effect of Biocontrol Bacteria Pseudomonas guar...

中国植物病理学会2019年学术年会

作者： Lin Nuoqiao zhang lianhui Guangdong Province Key Lab oratory of Microbial Signals and Disease Control Integrative Microbiology Research CentreSouth China Agricultural University

Sugarcane is an important cash crop in China,widely planted in Guangxi,Guangdong and Hainan *** smut,a fungal disease caused by Sporisorium scitamineum,is one of the vital diseases affecting quality and yield of *** of *** depends on the mating of bipolar sporidia to form a dikaryon and develops hyphae to penetrate the meristematic tissue of *** *** ST4,isolated from rhizosphere in Shantou city of Guangdong Province,was known to produce bioactive compounds to inhibit the mating process of *** and Ustilago maydis.A bioactive inhibitor produced by strain ST4 has been isolated and identified as an indole *** trial in greenhouse showed that control efficiency of this inhibitor on maize smut is up to 94%.In order to verify the field control effect of strain ST4 on sugarcane smut,sugarcane buds was soaked in strain ST4 solution prior to plantation in the field where the disease was serious the previous *** microbial agent was applied at the sowing stage and the tillering stage in order to compare the control effect of primary infection and re-infection of *** results showed that strain ST4 had better biocontrol effect on the re-infection of *** biennial root of sugarcane was also treated with strain *** results showed that the control effect of strain ST4 on sugarcane root was better than that of newly planted sugarcane,with biocontrol efficiency up to 76%.Optimization of treatment procedures might further improve the biocontrol efficiency.

关键词： Sugarcane smut Sporisorium scitamineum Pseudomonas guariconensis Strain ST4 Field trial

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微生物群体感应系统的研究进展

华南农业大学学报 2019年第5期40卷 50-58页

作者：张炼辉华南农业大学群体微生物研究中心

群体感应系统是一种微生物细胞细胞之间的通讯机制,是近年来微生物学领域的重大发现之一。从观察到个别微生物种群与细胞密度相关的自我诱导现象开始,到明确群体感应是微生物界广泛存在的重要的细胞细胞之间的通讯调控系统和相关系列应... 详细信息

群体感应系统是一种微生物细胞细胞之间的通讯机制,是近年来微生物学领域的重大发现之一。从观察到个别微生物种群与细胞密度相关的自我诱导现象开始,到明确群体感应是微生物界广泛存在的重要的细胞细胞之间的通讯调控系统和相关系列应用研究,其研究经历了偶然发现、范围扩大、理论升华和应用转化的过程。群体感应系统在微生物界普遍存在的证明,不仅为明确病原菌致病调控机理、阐明微生物生态竞争和动态平衡机制提供了一个全新的切入点,同时也为调控微生物生态体系和发展新的病害防控策略等奠定了坚实的基础。本文综述群体感应研究领域一些重要的和有代表性的发现,并讨论微生物群体感应系统研究的未来发展前景。

关键词：自我诱导群体感应细胞细胞通讯群体淬灭病害防控

Isolation and characterization of a azoxystrobin-degrading bacterial strain Ochrobactrum anthropi SH14

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Isolation and characterization of a azoxystrobin-degrading b...

政绩、工分与农药:20世纪50—70年代中国的农药施用

中国植物病理学会第十二届青年学术研讨会

作者： Shaohua Chen Yinyue Deng Fei He lianhui zhang Guangdong Province Key Laboratory of Microbial Signals and Disease Control South China Agricultural University Institute of Molecular and Cell Biology Agency for ScienceTechnology and Research(A*STAR) Department of Biological Sciences National University of Singapore

Continuous use of the strobilurin fungicide azoxystrobin(AZX) has resulted in serious environmental contamination *** report here that a novel bacterial strain SH14,which was isolated from wastewater treatment system using an enrichment culture technique,was able to degrade and utilize AZX as the carbon and nitrogen source in minimal *** on the morphology,physio-biochemical characteristics,16 S rDNA gene analysis,and API 20 NE systems,strain SH14 was identified as Ochrobactrum *** SH14 degraded 86.3%of 50 mgL

关键词： azoxystrobin Ochrobactrum anthropi biodegradation kinetics

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中国经济史研究 2023年第4期 133-146页

作者：张连辉中南财经政法大学经济学院、经济史学研究中心武汉430073

通过专业化组织施用农药,有利于提高农药施用效率和保证用药安全,有助于实现经济效益和环境效益的统一。20世纪50—70年代,中国政府将建立稳定的基层专业化施药组织作为保证施药安全与效率的重要措施进行推广。农业集体化生产理应更有... 详细信息

通过专业化组织施用农药,有利于提高农药施用效率和保证用药安全,有助于实现经济效益和环境效益的统一。20世纪50—70年代,中国政府将建立稳定的基层专业化施药组织作为保证施药安全与效率的重要措施进行推广。农业集体化生产理应更有利于推进施药专业化,但在20世纪60年代至70年代前期,很多生产队的施药组织却长期不稳定。农村基本核算单位的频繁变动、平均主义收入分配方式、运动式防治方式、重产出的政绩考核体系和偏低的施药工分等,均在不同程度上影响了施药组织的稳定性。其中,重产出的政绩考核体系和偏低的施药工分应是关键原因。本研究表明,将技术性和危险性纳入分析后,劳动项目报酬设定或预设的合理性,会影响到生产队劳动力资源的配置效率及其生态环境表现。从农药污染防治角度来看,建立在评工记分基础上的收入分配和生产管理制度,总体上具有非环境友好特征。群众性施药组织的稳定性何以会在20世纪70年代后期得到增强,尚待进一步研究。

关键词：生产队农药专业化防治工分政绩

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20世纪五六十年代中国的农药污染防治

中国经济史研究 2017年第2期 181-192页

作者：张连辉中南财经政法大学经济学院

20世纪50年代,中国在大规模使用化学农药之初,即开始积极探索农药污染的防治。因受到各种历史条件的制约,20世纪五六十年代的中国农药污染防治工作是在生产安全和劳动卫生理念下,围绕防治农药急性和亚急性中毒展开的,从而表现出明显的... 详细信息

20世纪50年代,中国在大规模使用化学农药之初,即开始积极探索农药污染的防治。因受到各种历史条件的制约,20世纪五六十年代的中国农药污染防治工作是在生产安全和劳动卫生理念下,围绕防治农药急性和亚急性中毒展开的,从而表现出明显的前现代性特征。这一时期的探索,为此后中国现代农药污染防治工作的起步,奠定了重要知识、技术和制度基础。这些探索的内容、特征与绩效,主要是由农业植保方针、农业发展政策、经济发展战略、经济发展水平等经济因素决定的。

关键词：中国植物保护农药污染 20世纪五六十年代