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Rice science 2024年第2期31卷 129-133,I0001-I0016页

作者： ZHANG Huali CHEN Junyu LI Ruiqing WANG Huimei DAI Dongqing LIANG Minmin WU Mingyue ma liangyong State Key Laboratory of Rice Biology and Breeding/Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006China

Two-line hybrid rice with excellent quality is preferred in the Chinese ***,there is a trade-off between reducing costs for hybrid seed production and lowering the outcrossing rate of the sterile line,which is largely determined by the stigma exsertion rate(SER).In this study,we constructed mutants of male sterility lines with improved grain length(GL)and SER in three elite early-season indica rice varieties through targeted manipulation of the TMS5 and GS3 genes using CRISPR/Cas9-mediated multiplex *** obtained a series of marker-free gs3 single mutants and gs3tms5 double mutants with significantly higher SER,longer grains,and increased 1000-grain weight compared with the wild type(WT).Importantly,the typically thermo-sensitive genic male sterile(TGMS)trait with a higher SER was observed in gs3tms5 mutants,and their F1 hybrids exhibited remarkable improvements in grain shape and yield-related *** findings provided an efficient method to generate new valuable TGMS germplasm with improved SER through the mutagenesis of GS3 and TMS5 synergistically,and demonstrated that GS3 had pleiotropic effects on grain size,SER,and grain quality in early-season indica rice.

关键词： sterile elite season

A 9.5-kb deletion in the 1st intron of OsmaDS51 enhances temperature sensitivity in rice

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The Crop Journal 2024年第4期12卷 1031-1040页

作者： Lei Zhao Haifei Hu Junyu Chen Chongrong Wang Yibo Chen Hong Li Daoqiang Huang Zhidong Wang Degui Zhou Rong Gong Yangyang Pan Junliang Zhao liangyong ma Shaochuan Zhou Rice Research Institute Guangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 310006ZhejiangChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510640GuangdongChina Key Laboratory of Genetics and Breeding of High Quality Rice in Southern China(Co-construction by Ministry and Province) Ministry of Agriculture and Rural AffairsGuangzhou 510640GuangdongChina Guangdong Rice Engineering Laboratory Guangzhou 510640GuangdongChina Guangdong Key Laboratory of New Technology in Rice Breeding Guangzhou 510640GuangdongChina

Temperature is an important environmental factor affecting heading date of *** its importance,genes responsible for temperature-sensitive heading in rice have remained *** previous study identified a quantitative trait locus qHd1 which advances heading date under high temperatures.A 9.5-kb insertion was found in the first intron of OsmaDS51 in indica variety Zhenshan 97(ZS97).However,the function of this natural variant in controlling temperature sensitivity has not been *** this study,we used CRISPR/Cas9 to knock out the 9.5-kb insertion in *** conducted under cotrolled conditions in phytotrons confirmed that deletion increased temperature sensitivity and advanced heading by downregulating the expression level of ***-hybrid assays in yeast,ChIP-quantitative polymerase chain reaction,electrophoretic mobility shift,and luciferase-based transient transactivation assays collectively confirmed that OsmaDS51 affects heading date by regulation of heading date gene *** further determined that the long non-coding RNA HEATINR is generated from the first intron of OsmaDS51,offering an explanation for how the 9.5-kb insertion affects temperature *** also found that OsmaDS51 was strongly selected in early/late-season rice varieties in South China,possibly accounting for their strong temperature *** insights not only advance our understanding of the molecular mechanisms underlying the temperature-responsive regulation of heading date in rice but also provide a valuable genetic target for molecular breeding.

关键词： OsmaDS51 Insertion Long non-coding RNA Oryza sativa

mapping QTLs for heading synchrony in a doubled haploid population of rice in two environments

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Journal of Genetics and Genomics 2009年第5期36卷 297-304页

作者： liangyong ma Changdeng Yang Dali Zeng Jing Cai Ximing Li Zhijuan Ji Yingwu Xia Qian Qian Jinsong Bao State Key Lab of Rice Biology China National Rice Research Institute Hangzhou 310006 China Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences Institute of Nuclear Agricultural Sciences Zhejiang University Hangzhou 310029 China

Simultaneous heading of plants within the same rice variety, also refer to heading synchrony, is an important factor that affects simultaneous ripening of the variety. Understanding of the genetic basis of heading synchrony may contribute to molecular breeding of rice with simultaneous heading and ripening. In the present study, a doubled haploid （DH） population, derived from a cross between Chunjiang 06 and TN1 was used to analyze quantitative trait locus （QTL） for heading synchrony related traits, i.e., early heading date （EHD）, late heading date （LHD）, heading asynchrony （HAS）, and tiller number （PN）. A total of 19 QTLs for four traits distributed on nine chromosomes were detected in two environments. One QTL, qHAS-8 for HAS, explained 27.7% of the phenotypic variation, co-located with the QTLs for EHD and LHD, but it was only significant under long-day conditions in Hangzhou, China. The other three QTLs, qHAS-6, qHAS-9, and qHAS-10, were identified under short-day conditions in Hainan, China, each of which explained about 11% of the phenotypic variation. Two of them, qHAS-6 and qHAS-9, were co-located with the QTLs for EHD and LHD. Two QTLs, qPN-4 and qPN-5 for PN, were detected in Hangzhou, and qPN-5 was also detected in Hainan. However, none of them was co-located with QTLs for EHD, LHD, and HAS, suggesting that PN and HAS were controlled by different genetic factors. The results of this study can be useful in marker assisted breeding for improvement of heading synchrony.

关键词： rice doubled haploid population heading synchrony quantitative trait loci (QTL)

Quantitative Trait Loci for Panicle Layer Uniformity Identified in Doubled Haploid Lines of Rice in Two Environments

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Journal of Integrative Plant Biology 2009年第9期51卷 818-824页

作者： liangyong ma Jinsong Bao Longbiao Guo Dali Zeng Ximing Li Zhijuan Ji Yingwu Xia Changdeng Yang Qian Qian State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China Key Laboratory of Zhejiang Province and Chinese Ministry of Agriculture for Nuclear-Agricultural Sciences Institute of Nuclear Agricultural Sciences Zhejiang University Hua Jiachi Campus Hangzhou 310029 China

Uniformity of stem height in rice directly affects crop yield potential and appearance, and has become a vital index for rice improvement. In the present study, a doubled haploid （DH） population, derived from a cross between japonica rice Chunjiang 06 and indica rice TN1 was used to analyze the quantitative trait locus （QTL） for three related traits of paniclelayer-uniformity; that is, the tallest panicle height, the lowest panicle height and panicle layer disuniforrnity in two locations：Hangzhou （HZ） and Hainan （HN）. A total of 16 QTLs for three traits distributed on eight chromosomes were detected in two different environments. Two QTLs, qTPH-4 and qTPH-8 were co-located with the QTLs for qLPH-4 and qLPH-8, which were only significant in the HZ environment, whereas the qTPH-6 and qLPH-6 located at the same interval were only significant in the HN environment. Two QTLs, qPLD-10.1 and qPLD-10.2, were closely linked to qTPH-10, and they might have been at the same locus. One QTL, qPLD-3, was detected in both environments, explaining more than 23% of the phenotypic variations. The CJ06 allele of qPLD-3 could increase the panicle layer disuniformity by 9.23 and 4.74 cm in the HZ and HN environments. Except for qPLD-3, almost all other QTLs for the same trait were detected only in one environment, indicating that these three traits were dramatically affected by environmental factors. The results may be useful for elucidation of the molecular mechanism of panicle-layer-uniformity and marker assisted breeding for super-rice.

关键词： double haploid population panicle layer uniformity plant height quantitative trait loci rice.

A backbone parent contributes core genomic architecture to pedigree breeding of early-season indica rice

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Journal of Genetics and Genomics 2021年第11期48卷 1040-1043页

作者： Junyu Chen Huali Zhang Shuhan Deng Huilong Du Zhuo Chen Yuhui Zhao Dongqing Dai Chengzhen Liang Ximing Li Chengzhi Liang Rui Zhang liangyong ma State Key Laboratory of Rice Biology and Chinese National Center for Rice Improvement China National Rice Research InstituteHangzhou 311400China Novogene Bioinformatics Institute Beijing 100083China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Biotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

Due to the capacity to deliver favorable target traits to offspring via breeder selection,backbone parents carrying accumulated favorable agronomic traits have been used widely in breeding programs in crop species,such as rice,wheat,cotton,and maize(Zhou et al.,2016;Fradgley et al.,2019;Li et al.,2019;ma et al.,2019;Han et al.,2020).It is estimated that 3656(~70%)of the major Chinese rice varieties released from the year 1950e2008 were found to be derived from as few as 35 backbone parents(Tang et al.,2012).Large-scale genome sequencing of diverse rice accessions has been emerged as a promising technology for the identification of key genomic regions or loci under selection during rice domestication(Huang et al.,2012;Wang et al.,2018)and genetic improvement(Xie et al.,2015).On the other hand,sequence information derived from breeding pedigrees could also help to unravel how favorable genomic regions were transmitted from parents to their offspring,as demonstrated by the researches on the pedigrees of the wellknown rice varieties Minghui 63 and Huanghuazhan(Zhou et al.,2016;Chen et al.,2017;Huang et al.,2018).These researches enhanced the understanding of the cumulative effects of beneficial alleles at limited loci in selected offspring varieties.A few examples include IPA1,Gn1a,GW5,GS3,and GS5 for high grain yield,Waxy for good grain eating quality,and Xa21 for high adversity adaptability.

关键词： breeding backbone season

基于增强CT影像组学预测浸润性肺腺癌EGFR突变状态的价值

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医学影像学杂志 2024年第5期34卷 61-66页

作者：王杰王振羽马良勇张金华林玉芬林吉征张濬韬山东省日照市岚山区人民医院放射科山东日照276807 华南理工大学微电子学院广东广州511442 青岛大学医学院附属医院放射科山东青岛266071 通用电气医疗精准医学研究院上海210000

目的探讨建立并验证影像组学诺模图预测浸润性肺腺癌表皮生长因子受体(EGFR)突变的能力。方法选取231例经病理证实为浸润性肺腺癌患者,并有EGFR突变资料,按照7∶3的比例分为训练集(n=162)和测试集(n=69)。利用最大相关最小冗余(mRMR)及... 详细信息

目的探讨建立并验证影像组学诺模图预测浸润性肺腺癌表皮生长因子受体(EGFR)突变的能力。方法选取231例经病理证实为浸润性肺腺癌患者,并有EGFR突变资料,按照7∶3的比例分为训练集(n=162)和测试集(n=69)。利用最大相关最小冗余(mRMR)及最小绝对收缩与选择子算法(LASSO)筛选最佳影像组学特征,构建预测肺腺癌EGFR突变状态的模型;以单因素及多因素logistic回归筛选浸润性肺腺癌EGFR突变相关的临床、病理及CT特征,构建临床模型;联合影像组学特征及临床、病理、CT特征构建影像组学诺模图,ROC曲线及AUC值评估模型的预测效能,并采用DeLong检验比较模型间AUC值差异是否有统计学意义,校正曲线及DCA曲线评估影像组学诺模图的临床价值。结果最终基于增强扫描动脉期及静脉期筛选出13项影像组学特征建立影像组学模型,该模型在训练集及测试集中的AUC值为0.79、0.76;临床模型由性别、病理分期及血管集束征构成,该模型在训练集及测试集中的AUC值分别为0.75、0.75;影像组学诺模图在训练集及测试集中的AUC值分别为0.82、0.80;DeLong检验显示,训练集中影像组学诺模图AUC值大于临床模型,差异有统计学意义(P0.05);测试集中3种模型AUC值差异均无统计学意义(P>0.05);影像组学诺模图在训练集及测试集中的拟合优度良好,DCA曲线显示影像组学诺模图临床效用优于临床模型。结论基于增强CT影像组学诺模图对浸润性肺腺癌EGFR突变状态有良好的预测能力。

关键词：影像组学浸润性肺腺癌表皮生长因子受体体层摄影术 X线计算机

High-yielding rice variety Zhong 83-49

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Chinese Rice Research Newsletter 1991年第2期 1-2页

作者： WU Menglan ZHAO Meiling ZHUANG Jieyun ma liangyong LI Ximing CNRRI,Hangzhou 310006,China

The early season indica rice variety Zhong83-49 was developed in 1981 as one of theFbreeding lines by selections from IR24/Yuanfengzao(F)//Zhuke *** various tri-als and tests,Zhong 83-49 has been proved to be a

关键词： High-yielding rice variety Zhong 83-49

High-yielding rice variety Zhong 83-49

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Rice Science 1991年第3期 1-2页

作者： WU Menglan ZHAO Meiling ZHUANG Jieyun ma liangyong LI Ximing CNRRI,Hangzhou 310006,China

关键词： High-yielding rice variety Zhong 83-49

mapping of Wbph6(t)-a new gene resistant to white-backed planthopper

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Chinese Rice Research Newsletter 2001年第4期9卷 2-3页

作者： ma liangyong ZHUANG Jieyun LIU Grannie MIN Shaokai LI Ximing CNRRI Hangzhou 310006,China CNRRI Hangzhou 310006China

Whitebacked planthopper (WBPH,Sogatella furcifera Horvath) is one ofthe most destructive insects for *** utilization of WBPH resistancegenes is always an efficient solution tothis problem. Besides five WBPHresistance ... 详细信息

关键词： TNI mapping of Wbph6 a new gene resistant to white-backed planthopper gene

From Green Super Rice to green agriculture:Reaping the promise of functional genomics research

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Molecular Plant 2022年第1期15卷 9-26页

作者： Sibin Yu Jauhar Ali Shaochuan Zhou Guangjun Ren Huaan Xie Jianlong Xu Xinqiao Yu Fasong Zhou Shaobing Peng liangyong ma Dingyang Yuan Zefu Li Dazhou Chen Ruifeng Zheng Zhigang Zhao Chengcai Chu Aiqing You Yu Wei Susong Zhu Qiongyao Gu Guangcun He Shigui Li Guifu Liu Changhua Liu Chaopu Zhang Jinghua Xiao Lijun Luo Zhikang Li Qifa Zhang National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China International Rice Research Institute DAPO Box 7777Metro ManilaPhilippines Guangdong Academy of Agricultural Sciences GuangzhouChina Sichuan Academy of Agricultural Sciences ChengduChina Fujian Academy of Agricultural Sciences FuzhouChina Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijingChina Shanghai Agrobiological Gene Center ShanghaiChina China National Seed Group Co. LtdBeijingChina China National Rice Research Institute HangzhouChina Hunan National Hybrid Rice Center ChangshaChina Anhui Academy of Agricultural Sciences HefeiChina Jiangxi Academy of Agricultural Sciences NanchangChina Nanjing Agricultural University NanjingChina State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina Hubei Academy of Agricultural Sciences WuhanChina Guangxi Academy of Agricultural Sciences NanningChina Guizhou Academy of Agricultural Sciences GuiyangChina Yunnan Academy of Agricultural Sciences KunmingChina Wuhan University WuhanChina Sichuan Agricultural University ChengduChina Heilongjiang Academy of Agricultural Sciences HarbinChina College of Agronomy Anhui Agricultural UniversityHefeiChina Wuhan Greenfafa Institute of Novel Genechip R&D China

Producing sufficient food with finite resources to feed the growing global population while having a smaller impact on the environment has always been a great ***,we review the concept and practices of Green Super Rice(GSR)that have led to a paradigm shift in goals for crop genetic improvement and models of food production for promoting sustainable *** momentous achievements and global deliveries of GSR have been fueled by the integration of abundant genetic resources,functional gene discoveries,and innovative breeding techniques with precise gene and whole-genome selection and efficient agronomic management to promote resource-saving,environmentally friendly crop production *** also provide perspectives on new horizons in genomic breeding technologies geared toward delivering green and nutritious crop varieties to further enhance the development of green agricul-ture and better nourish the world population.

关键词： rice functional genomics Green Super Rice genomic breeding nutritious crops sustainable agriculture