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Comparative genetic analysis of Arabidopsis purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation

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Journal of Integrative Plant Biology 2014年第3期56卷 299-314页

作者： liangsheng wang Shan Lu Ye Zhang Zheng Li Xiaoqiu Du Dong Liu MOE Key Laboratory of Bioinformatics Center for Plant Biology School of Life Sciences Tsinghua University

Induction and secretion of acid phosphatases （APases） is thought to be an adaptive mechanism that helps plants survive and grow under phosphate （Pi） deprivation, in Arabidopsis, there are 29 purple acid phosphatase （AtPAP） genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock-down T-DNA lines for all 29 AtPAP genes. Using these atpap mutants combined with in-gel and quantitative APase enzyme assays, we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in Arabidopsis while AtPAPlo is mainly a secreted APase. On Pi-deficient （P-） medium or P- medium supplemented with the organophosphates ADP and fructose-6-phosphate （Fru-6-P）, growth of atpaplo was significantly reduced whereas growth of atpap12 was only moderately reduced, and growth of atpap26 was nearly equal to that of the wild type （WT）. Overexpression of the AtPAP12 or AtPAP26 gene, however, caused plants to grow better on P- or P- medium supplemented with ADP or Fru-6-P. Interest-ingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.

关键词： Phosphate starvation responses acid phosphatase activity atpap mutants~ AtPAP overexpression~ tolerance to phosphatedeprivation

The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony

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Horticulture Research 2023年第7期10卷 69-86页

作者： Jin Zhu Yizhou wang Qianyu wang Bing Li Xiaohan wang Xian Zhou Hechen Zhang Wenzhong Xu Shanshan Li liangsheng wang Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093 China University of Chinese Academy of Sciences Beijing 100049China Horticulture Research Institute Henan Academy of Agricultural SciencesZhengzhou 450002China

Xibei tree peony is a distinctive cultivar group that features red–purple blotches in ***,the pigmentations of blotches and non-blotches are largely independent of one *** underlying molecular mechanism had attracted lots of attention from investigators,but was still *** present work demonstrates the factors that are closely related to blotch formation in Paeonia rockii‘Shu Sheng Peng Mo’.Non-blotch pigmentation is prevented by the silencing of anthocyanin structural genes,among which PrF3H,PrDFR,and PrANS are the three major *** characterized two R2R3-MYBs as the key transcription factors that control the early and late anthocyanin biosynthetic ***1,which belongs to MYB subgroup 7(SG7)was found to activate the early biosynthetic gene(EBG)PrF3H by interacting with SG5 member PrMYBa2 to form an‘MM’*** SG6 member PrMYBa3 interacts with two SG5(IIIf)bHLHs to synergistically activate the late biosynthetic genes(LBGs)PrDFR and PrANS,which is essential for anthocyanin accumulation in petal *** comparison of methylation levels of the PrANS and PrF3H promoters between blotch and non-blotch indicated a correlation between hypermethylation and gene *** methylation dynamics of PrANS promoter during flower development revealed a potential early demethylating reaction,which may have contributed to the particular expression of PrANS solely in the blotch *** suggest that the formation of petal blotch may be highly associated with the cooperation of transcriptional activation and DNA methylation of structural gene promoters.

关键词： belongs synergistic pigment

Biogenesis of flavor-related linalool is diverged and genetically conserved in tree peony (Paeonia × suffruticosa)

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Horticulture Research 2023年第2期10卷 15-27页

作者： Shanshan Li Ling Zhang Miao Sun Mengwen Lv Yong Yang Wenzhong Xu liangsheng wang Key Laboratory of Plant Resources Institute of BotanyChinese Academy of SciencesBeijing 100093China China National Botanical Garden Beijing 100093China University of Chinese Academy of Sciences Beijing 100049China School of Landscape Architecture Beijing Forestry UniversityBeijing 100083China

Floral scent is an important and genetically complex trait in horticultural *** peony(Paeonia×suffruticosa)originates in the Pan-Himalaya and has nine wild species divided into two subsections,Delavayanae and *** f lowers are beloved worldwide for their sweet f loral fragrance,yet the f lavor-related volatiles and underlying biosynthetic pathways remain ***,we characterized the volatile blends of all wild tree peony species and found that the flavor-related volatiles were highly divergent,but linalool was a unique monoterpene in *** detection of volatiles in 97 cultivars with various genetic backgrounds showed that linalool was also the characteristic aroma component in Paeonia delavayi hybrid progenies,suggesting that linalool was conserved and dominant within *** and its hybrids,instead of species and cultivars from *** transcriptome analysis of all wild tree peony species and 60 cultivars revealed five candidate genes that may be involved in key steps of linalool biosynthesis;especially the expressions of three TPS genes,PdTPS1,PdTPS2,and PdTPS4,were significantly positively correlated with linalool emissions across tree peony *** biochemical evidence demonstrated that PdTPS1 and PdTPS4were the pivotal genes determining the species-specific and cultivar-specific emission of *** study revealed a new insight into floral scent divergence in tree peony and would greatly facilitate our understanding of the phylogeny and evolution of Paeonia.

关键词： conserved tree steps

Chemical Mechanism of Flower Color Microvariation in Paeonia with Yellow Flowers

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Horticultural Plant Journal 2020年第3期6卷 179-190页

作者： Yong Yang Bing Li Chengyong Feng Qian Wu Qianyu wang Shanshan Li Xiaonan Yu liangsheng wang Key Laboratory of Plant Resources/Beijing Botanical Garden Institute of BotanyChinese Academy of SciencesBeijing 100093China Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding National Engineering Research Center for FloricultureCollege of Landscape ArchitectureBeijing Forestry UniversityBeijing 100083China University of Chinese Academy of Sciences Beijing 100049China

Paeonia species are important ornamental plants,including three types:tree peony,herbaceous peony,and Itoh hybrid *** with yellow color is desired and becoming ***,the chemical mechanism of the formation of yellow flowers in Paeonia is still *** this study,14 representative samples were selected:four samples of three species of Paeonia,four herbaceous peony cultivars,three lutea hybrid tree peony cultivars,and three Itoh hybrid peony *** petal extracts of the samples were determined by HPLC-DAD and HPLCQ-TOF-MS/*** total,29 flavonoids were isolated and identified,including 28 flavonol glycosides and one chalcone *** were significant differences in the composition and content of flavonoids in petals of different cultivars(species).The total content of flavonoids was between 19.430 and 143.043 mg·g^−1 *** glycosides were detected in all *** 2′-O-glucoside was detected from one herbaceous peony cultivar‘Golden Wheel’lutea hybrid tree peony cultivars,and Itoh peony *** content of chalconaringenin 2′-O-glucoside in the samples was more than 43% of total *** pigment type and content of lutea hybrid tree peonies and Itoh hybrid peonies are *** flavonoids,especially quercetin 3-O-galloylglucoside and chalconaringenin 2′-O-glucoside contribute greatly to the formation of yellow flowers of *** results of this study provide a comprehensive understanding of the chemical mechanism for yellow flower coloration of Paeonia,and lay the foundation for yellow flower breeding of Paeonia.

关键词： Paeonia Yellow flower HPLC Flavonoid composition Breeding

Quercetin-derivatives painting the yellow petals of American lotus(Nelumbo lutea)and enzymatic basis for their accumulation

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Horticultural Plant Journal 2023年第1期9卷 169-182页

作者： Qingqing Liu Dasheng Zhang Fengluan Liu Zhuoxing Liu Xiaohan wang Yong Yang Shanshan Li Hanchun Li Daike Tian liangsheng wang Key Laboratory of Plant Resources/Beijing Botanical Garden Institute of BotanyChinese Academy of SciencesBeijing 100093China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical GardenShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China

American lotus(Nelumbo lutea)is one of the two species in Nelumbo and has only yellow *** of total flavonoids showed wild American lotus contained almost only flavonols with quercetin 3-O-glucuronide to be the dominant *** variation tendency of the total flavonol content was coincident with yellow color variation of petals during flower *** understand the mechanism of accumulation and constituent of pigments in petals,three pivotal genes,NlFLS1,NlFLS2 and NlFLS3,which were predicted to encode flavonol synthases were isolated and characterized by analyses of basic bioinformatics,temporal and spatial expression patterns and enzymatic *** temporal expression levels showed the same variation tendency,which was also consistent with the development-dependent variation of total flavonol *** expression patterns indicated the three genes should function in *** the three proteins were demonstrated to be bifunctional dioxygenase,possessing both flavonol synthase activity and flavanone 3-hydroxylase ***,other flavonol biosynthesis related genes were also investigated on their expression levels to give more clues on the *** preferences of the three FLSs,substrate competitions between the FLSs and other flavonol biosynthesis related enzymes,and the greatly differential expression levels between F3’H(flavonoid 3-hydroxylase)and F3’5’H(flavonoid 3,5-hydroxylase)contributed to the flavonol constituent in the petals of America lotus,namely abundant quercetin-derivatives while very few kaempferol-derivatives and myricetin-derivatives.

关键词： Lotus Nelumbo Flavonoid Flavonol Quercetin

Flow Behavior and Evolution of Microstructure during Hot Deformation for a High Mo Stainless Steel

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Journal of Materials Science & Technology 2000年第3期16卷 341-344页

作者： Yourong XU, liangsheng CHEN, Deying wang and Lei JIN (School of Materials Science and Engineering, Shanghai University, Shanghai 201800, China) School of Materials Science and Engineering Shanghai University Shanghai 201800 China

The mechanical behaviors of high Mo austenitic stainless steel OOCr20Nil8Mo6Cu[N] have been investigated using the methods of hot compression simulation test on the Thermecmaster-Z simulator. The dynamic recrystallization kinetic equation was established, Avrami coefficient n lies in between 0.9～2 depending on deformation parameters. A perfect flow stress model considering dynamic recrystallization was also established. Dynamic recrystallization tends to complete at 1050℃ and high strain rate, but at temperature below 950℃, it is hard to occur. Double-stage interrupt compression tests were carried out. Activation energy for static and metadynamic re-crystallization have been obtained respectively (QSRX=483.7, QMDEX=253.5 kJ/mol). Avrami coefficient of MDRX is about 0.5, and t.0.5-kinetics equations of SRX and MDRX have also been constructed. The evolution of microstructures during interrupt compression deformation was investigated. Static and metadynamic recrystaltlzation is essential to improve plasticity, at temperature above 1000℃ increasing interpass time has advantage for static and metadynamic recrystallization.

关键词： Stainless steel

Germplasm resources and genetic breeding of Paeonia:a systematic review

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Horticulture Research 2020年第1期7卷 1369-1387页

作者： Yong Yang Miao Sun Shanshan Li Qihang Chen Jaime A.Teixeira da Silva Ajing wang Xiaonan Yu liangsheng wang Key Laboratory of Plant Resources/Beijing Botanical Garden Institute of BotanyChinese Academy of Sciences100093 BeijingChina College of Landscape Architecture Beijing Forestry University100083 BeijingChina Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding 100083 BeijingChina National Engineering Research Center for Floriculture 100083 BeijingChina University of Chinese Academy of Sciences 100049 BeijingChina P.O.Box 7 Miki–cho post officeIkenobe 3011–2Kagawa–ken 761–0799Japan

Members of the genus Paeonia,which consists of globally renowned ornamentals and traditional medicinal plants with a rich history spanning over 1500 years,are widely distributed throughout the Northern *** 1900,over 2200 new horticultural Paeonia cultivars have been created by the discovery and breeding of wild ***,information pertaining to Paeonia breeding is considerably fragmented,with fundamental gaps in knowledge,creating a bottleneck in effective breeding *** review systematically introduces Paeonia germplasm resources,including wild species and cultivars,summarizes the breeding strategy and results of each Paeonia cultivar group,and focuses on recent progress in the isolation and functional characterization of structural and regulatory genes related to important horticultural *** pertaining to the resource protection and utilization,breeding and industrialization of Paeonia in the future are also briefly discussed.

关键词： breeding cultivar globally

Agrobacterium tumefaciens-Mediated Transformation of Wild Tobacco Species Nicotiana debneyi, Nicotiana clevelandii, and Nicotiana glutinosa

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American Journal of Plant Sciences 2016年第1期7卷 1-7页

作者： Wei Duan liangsheng wang Guoqing Song Key Laboratory of Plant Resources/Beijing Key Laboratory of Grape Science and Enology Institute of Botany Chinese Academy of Sciences Beijing China Key Laboratory of Plant Resources/Beijing Botanical Garden Institute of Botany Chinese Academy of Sciences Beijing China Plant Biotechnology Resource and Outreach Center Department of Horticulture Michigan State University East Lansing USA

Studies on Agrobacterium tumefaciens-mediated transformation of wild tobaccos Nicotiana debneyi, Nicotiana clevelandii, and Nicotiana glutinosa were conducted. Leaf disks were infected and co-cultivated with A. tumefaciens strain EHA105 carrying the binary vector pBISN1 with an intron interrupted β-glucuronidase (GUS) reporter gene (gusA) and the neomycin phosphotransferase gene (nptII). Selection and regeneration of kanamycin resistant shoots were conducted on regeneration medium containing 8.88 μM 6-benzylaminopurine (BAP), 0.57 μM indole-3-acetic acid (IAA), 50 mg·L-1 kanamycin and 250 mg·L-1 timentin. Kanamycin resistant shoots were rooted Murashige and Skoog (MS) medium containing 100 mg·L-1 kanamycin and 250 mg·L-1 timentin. Using this protocol, kanamycin-resistant plants were obtained from all three wild tobaccos at frequencies of 75.6% for N. debneyi, 25.0% for N. clevelandii, and 2.8% for N. glutinosa. Transcripts of nptII and gusA were detected in kanamycin-resistant T0 transformants (i.e., 2 for N. glutinosa and 5 for each of the N. debneyi and N. clevelandii) by the reverse transcript polymerase chain reaction (RT-PCR), and histochemical GUS assays confirmed expression of gusA in both T0 plants and T1 seedlings. The results indicate that the protocols are efficient for transformation of wild tobacco N. debneyi and N. clevelandii.

关键词： Regeneration Transformation Transgenic Plant Nicotiana

Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals

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Horticultural Plant Journal 2020年第6期6卷 428-438页

作者： Zhenzhu Fu Hongquan Shang Hui Jiang Jie Gao Xiaoyu Dong Huijuan wang Yanmin Li Limin wang Jing Zhang Qingyan Shu Yacong Chao Menglan Xu Rui wang liangsheng wang Hechen Zhang Horticultural Research Institute Henan Academy of Agricultural SciencesZhengzhou 450002China Key Laboratory of Plant Resources/Beijing Botanical Garden Institute of BotanyThe Chinese Academy of SciencesBeijing 100093China College of Forestry Henan Agricultural UniversityZhengzhou 450002China

Previous studies have shown that high light intensity can induce anthocyanin synthesis(AS)in petunia *** identifywhich kind of light quality plays a role in inducing such metabolic process,and what transcripts participate in controlling it,we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality *** the red and white light treatments,a total of 2205 differentially expressed genes and 15,22,and 20 differentially expressed circRNAs,miRNAs,and lncRNAs,were identified *** AS-related genes,including the structural genes CHSj,F3H,F35H,DFR,and ANS,and the regulatory genes AN4,DPL,PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light ***,the light photoreceptor Cryptochrome 3(CRY3)and a series of light-dependent genes,such as PIF,HY5,andBBXs,were also determined to respond to the light *** anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue *** results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light *** two-hybrid results showed that the key elements in the light signal pathway,HY5 can interact with BBX19,BBX24 and *** PHZ,the important AS regulator can induce anthocyanin synthesis in response to blue light quality fromtransient expression analysis in petunia *** findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis,but also allow us to explore potential target genes for improving pigment production in petunia flower petals.

关键词： Petunia Anthocyanin Transcription factor Light quality Transcriptome analysis