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The RNA binding protein EHD6 recruits the m^(6)A reader YTH07 and sequesters OsCOL4 mRNA into phase-separated ribonucleoprotein condensates to promote rice flowering

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Molecular Plant 2024年第6期17卷 935-954页

作者： Song Cui Peizhe Song Chaolong Wang Saihua chen Benyuan Hao Zhuang Xu Liang Cai Xu chen Shanshan Zhu Xiangchao Gan Hui Dong Yuan Hu Liang Zhou Haigang Hou Yunlu Tian Xi Liu liangming chen Shijia Liu Ling Jiang Haiyang Wang Guifang Jia Shirong Zhou Jianmin Wan State Key Laboratory for Crop Genetics&Germplasm Enhancement and Utilization Zhongshan Biological Breeding LaboratoryNational Observation and Research Station of Rice Germplasm ResourcesNanjing Agricultural UniversityNanjing 210095China State Key Laboratory for Crop Gene Resources and Breeding Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China Synthetic and Functional Biomolecules Center Beijing National Laboratory for Molecular SciencesKey Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of EducationCollege of Chemistry and Molecular EngineeringPeking-Tsinghua Center for Life SciencesBeijing Advanced Center of RNA BiologyPeking UniversityBeijingChina Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural College of Yangzhou UniversityYangzhou 225009China Max Planck Institute for Plant Breeding Research Carl-von-Linne´-Weg 1050829 KolnGermany

N6-Methyladenosine(m^(6)A)is one of the most abundant modifications of eukaryotic mRNA,but its comprehensive biological functionality remains further *** this study,we identified and characterized a new flowering-promoting gene,EARLY HEADING DATE6(EHD6),in ***6 encodes an RNA recognition motif(RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein(RNP)granules and can bind both m^(6)A-modified RNA and unmodified RNA *** found that EHD6 can physically interact with YTH07,a YTH(YT521-B homology)domain-containing m^(6)A *** showed that their interaction enhances the binding of an m^(6)A-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated *** these condensates,the mRNA of a rice flowering repressor,CONSTANS-like 4(OsCOL4),becomes sequestered,leading to a reduction in its protein abundance and thus accelerated flowering through the Early heading date 1 *** together,these results not only shed new light on the molecular mechanism of efficient m^(6)A recognition by the collaboration between an RNA binding protein and YTH family m^(6)A reader,but also uncover the potential for m^(6)A-mediated translation regulation through phaseseparated ribonucleoprotein condensation in rice.

关键词： N6-methyladenosine m^(6)A EHD6 YTH domain-containing protein phase separation heading date

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慢生活理念下乡村景观规划设计评价体系的构建及分析

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绿色科技 2024年第9期26卷 45-51,60页

作者：张诗琪陈亮明中南林业科技大学林学院湖南长沙410004

以长沙县鹿芝岭村为研究对象,运用层次分析法建立慢生活理念下乡村景观规划设计评价体系,探究慢生活理念下乡村景观规划设计中各项影响要素之间的关系。通过分析鹿芝岭村现状,从构建品质型乡村空间、生态环境、地域文化、特色经济产业... 详细信息

以长沙县鹿芝岭村为研究对象,运用层次分析法建立慢生活理念下乡村景观规划设计评价体系,探究慢生活理念下乡村景观规划设计中各项影响要素之间的关系。通过分析鹿芝岭村现状,从构建品质型乡村空间、生态环境、地域文化、特色经济产业、民主性慢意识5个方面,选取景观营造程度、绿化覆盖率、生态自然环境风貌、人居环境等17个评价指标。结果表明:构建品质型乡村空间(0.3802)最为重要,其次为生态环境(0.1927)、地域文化(0.1885)以及特色经济产业(0.1667),民主性慢意识(0.0719)作为最后考虑的因素。研究结果可以为慢生活理念下同类型乡村景观规划设计提供科学依据与参考。

关键词：慢生活理念乡村景观规划设计层次分析法长沙县鹿芝岭村

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基于CiteSpace的“乡愁”景观研究可视化分析

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绿色科技 2024年第9期26卷 22-29页

作者：杜朝阳陈亮明中南林业科技大学林学院、水土保持学院湖南长沙410004

借助CiteSpace分析软件,以2013-2023年中国知网(CNKI)收录的期刊论文为对象,围绕“乡愁”为主题的景观规划相关文献的发文量、期刊、作者及关键词进行共现和聚类分析,并得到可视化图谱,从而探索该领域的研究现状、研究热点和研究发展趋... 详细信息

借助CiteSpace分析软件,以2013-2023年中国知网(CNKI)收录的期刊论文为对象,围绕“乡愁”为主题的景观规划相关文献的发文量、期刊、作者及关键词进行共现和聚类分析,并得到可视化图谱,从而探索该领域的研究现状、研究热点和研究发展趋势,为“乡愁”景观的规划研究提供参考。研究结果表明:在现有的研究成果集中,高质量的研究成果数量有限。主要的研究机构为高校,且作者间呈现出相对独立的研究态势。研究主题集中度较高,初期研究主要聚焦于城镇化问题,随后“乡村振兴”逐渐成为“乡愁”景观规划领域的研究热点,预计将继续成为未来研究的重点领域。通过运用CiteSpace软件对“乡愁”景观规划的发展现状及未来趋势进行深入探索与分析,对于我国未来景观规划研究的发展具有重要的实际意义。

关键词：乡愁景观乡村振兴 CiteSpace 可视化分析

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Maneuvering Angle Rigid Formations With Global Convergence Guarantees

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IEEE/CAA Journal of Automatica Sinica 2022年第8期9卷 1464-1475页

作者： liangming chen Zhiyun Lin Hector Garcia de Marina Zhiyong Sun Mir Feroskhan School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingapore 639798Singapore Department of Computer Architecture and Technology and also with CITICUniversity of Granada18014 GranadaSpain Department of Electrical Engineering Eindhoven University of Technology5600 MB Eindhoventhe Netherlands

Angle rigid multi-agent formations can simultaneously undergo translational,rotational,and scaling maneuvering,therefore combining the maneuvering capabilities of both distance and bearing rigid ***,maneuvering angle rigid formations in 2D or 3D with global convergence guarantees is shown to be a challenging problem in the existing literature even when relative position measurements are *** by angle-induced linear equations in 2D triangles and 3D tetrahedra,this paper aims to solve this challenging problem in both 2D and3D under a leader-follower *** the 2D case where the leaders have constant velocities,by using local relative position and velocity measurements,a formation maneuvering law is designed for the followers governed by double-integrator *** the leaders have time-varying velocities,a sliding mode formation maneuvering law is proposed by using the same *** the 3D case,to establish an angle-induced linear equation for each tetrahedron,we assume that all the followers'coordinate frames share a common Z ***,a formation maneuvering law is proposed for the followers to globally maneuver Z-weakly angle rigid formations in *** extension to Lagrangian agent dynamics and the construction of the desired rigid formations by using the minimum number of angle constraints are also *** examples are provided to validate the effectiveness of the proposed algorithms.

关键词： Index Terms—Angle rigid formations formation control formation maneuvering global convergence multi-agent systems

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华中樱叶绿体基因组特征与分子标记

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中南林业科技大学学报 2024年第1期44卷 175-184页

作者：阳秀玫陈亮明禹霖柏文富李建挥吴思政李柏海熊颖聂东伶严佳文中南林业科技大学林学院湖南长沙410004 湖南省植物园湖南长沙410116

【目的】组装华中樱叶绿体基因组并进行序列特征分析和分子标记初步鉴定。【方法】分别应用SPAdes、prodigal v2.6.3、hmmer v3.1b2及aragorn v1.2.38软件进行华中樱叶绿体基因组组装、基因编码区注释、r RNA和t RNA预测。应用OGDRAW软... 详细信息

【目的】组装华中樱叶绿体基因组并进行序列特征分析和分子标记初步鉴定。【方法】分别应用SPAdes、prodigal v2.6.3、hmmer v3.1b2及aragorn v1.2.38软件进行华中樱叶绿体基因组组装、基因编码区注释、r RNA和t RNA预测。应用OGDRAW软件进行叶绿体基因组图谱绘制。运用DNAMAN软件对26个樱亚属物种和华中樱的叶绿体基因组序列进行多重比对,人工检索华中樱特有的SNP和InDel位点。应用Codon W1.4.2和EMBOSS的cusp软件分析叶绿体基因组中基因的相对同义密码子使用度。【结果】华中樱叶绿体基因组大小为158 019 bp,其中小单拷贝区(Small single copy,SSC)、大单拷贝区(Large single copy,LSC)和反向重复区(Inverted repeat,IR)大小分别为19 247、85 910和52 862 bp,总GC含量为36.7%。华中樱叶绿体基因组共编码130个基因,包括8个核糖体RNA基因、37个转运RNA基因以及85个蛋白编码基因。序列中有SSR位点240个,其中单核苷酸重复位点153个,二核苷酸重复位点12个,三核苷酸重复位点63个,四核苷酸重复位点11个,六核苷酸重复位点1个。通过与另外26种樱亚属植物叶绿体基因组序列进行多重比对,鉴定了华中樱潜在的种特异性SNP位点29个,InDel标记8个。对密码子进行统计可知,该物种的66种密码子编码了21种氨基酸,其中RSCU> 1的密码子共有32个,有29个以A/U结尾,这些密码子结尾具有A/U偏好性。【结论】华中樱叶绿体基因组是典型的环状四分体结构,其与其他蔷薇科植物叶绿体基因组大小相近。挖掘了一批SSR、SNP和InDel标记,可为华中樱谱系地理学、群体遗传学和种间杂种鉴定等研究提供参考。

关键词：华中樱叶绿体基因组 SSR InDel

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Spacecraft formation-containment flying control with time-varying translational velocity

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Chinese Journal of Aeronautics 2020年第1期33卷 271-281页

作者： liangming chen Chuanjiang LI Yanning GUO Guangfu MA Baolong ZHU Department of Control Science and Engineering Harbin Institute of TechnologyHarbin 150001China School of Electrical Engineering and Automation Qilu University of Technology(Shandong Academy of Sciences)Jinan 250353China

This paper investigates the problem of Spacecraft Formation-Containment Flying Control(SFCFC)when the desired translational velocity is *** SFCFC problem,there are multiple leader spacecraft and multiple follower spacecraft and SFCFC can be divided into leader spacecraft’s formation control and follower spacecraft’s containment ***,under the condition that only a part of leader spacecraft can have access to the desired time-varying translational velocity,a velocity estimator is designed for each leader ***,based on the estimated translational velocity,a distributed formation control algorithm is designed for leader spacecraft to achieve the desired formation and move with the desired translational velocity ***,to ensure all follower spacecraft converge to the convex hull formed by the leader spacecraft,a distributed containment control algorithm is designed for follower ***,to reduce the dependence of the designed control algorithms on the graph information and increase system robustness,the control gains are changing adaptively and the parametric uncertainties are handled,***,simulation results are provided to illustrate the effectiveness of the theoretical results.

关键词： Adaptive gain technique Distributed control Formation-containment Formation control Spacecraft formation flying

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Design and analysis of recurrent neural network models with non-linear activation functions for solving time-varying quadratic programming problems

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CAAI Transactions on Intelligence Technology 2021年第4期6卷 394-404页

作者： Xiaoyan Zhang liangming chen Shuai Li Predrag Stanimirovic Jiliang Zhang Long Jin School of Information Science and Engineering Lanzhou UniversityLanzhouChina Faculty o£Sciences and Mathematics University of NisNisSerbia Department of Electronic and Electtical Engineering The University of SheffieldSheffieldUK

A special recurrent neural network(RNN),that is the zeroing neural network(ZNN),is adopted to find solutions to time-varying quadratic programming(TVQP)problems with equality and inequality ***,there are some weaknesses in activation functions of traditional ZNN models,including convex restriction and redundant *** the aid of different activation functions,modified ZNN models are obtained to overcome the drawbacks for solving TVQP *** and experimental research indicate that the proposed models are better and more effective at solving such TVQP problems.

关键词： activation programming quadratic

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Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants

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Plant Communications 2023年第1期4卷 1-12页

作者： Yunlong Wang Yihua Wang Xiaopin Zhu Yulong Ren Hui Dong Erchao Duan Xuan Teng Huanhuan Zhao Rongbo chen Xiaoli chen Jie Lei Hang Yang Yunlu Tian liangming chen Xi Liu Shijia Liu Ling Jiang Haiyang Wang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095P.R.China National Key Facility for Crop Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of *** studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains *** this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun *** further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid *** addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 ***,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun ***,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde *** together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.

关键词： tetrapyrrole biosynthesis plastid gene expression plastid retrograde signal

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Genetic dissection of top three leaf traits in rice using progenies from a japonica × indica cross

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Journal of Integrative Plant Biology 2017年第12期59卷 866-880页

作者： Changbin Yin Huihui Li Zhigang Zhao Zhiquan Wang Shijia Liu liangming chen Xi Liu Yunlu Tian Juan Ma Lidong Xu Dashuang Zhang Susong Zhu Danting Li Jianmin Wan Jiankang Wang The National Key Facility for Crop Gene Resources and Genetic Improvement and Institute of Crop ScienceChinese Academy of AgriculturalSciencesBeijing 100081China National Key Laboratory for Crop Genetics and Germplasm Enhancement Research Center of Jiangsu Plant Gene EngineeringNanjingAgricultural UniversityNanjing 210095China Rice Research Institute Jiangxi Academy of Agricultural SciencesNanchang 330200China Rice Research Institute Guizhou Academy of Agricultural SciencesGuiyang 550006China Rice Research Institute Guangxi Academy of Agricultural SciencesNanning 530007China

The size of the top three leaves of rice plants is strongly associated with yield; thus, it is important to consider quantitative traits representing leaf size （e.g., length and width） when breeding novel rice varieties. It is challenging to measure such traits on a large scale in the field, and little is known about the genetic factors that determine the size of the top three leaves. In the present study, a population of recombinant inbred lines （RILs） and reciprocal single chromosomal segment substitution lines （SSSLs） derived from the progeny of a japonica Asominori x indica IR24 cross were grown under four diverse environmental conditions. Six morphological traits associated with leaf size were measured,namely length and width of the flag, second and third leaves. In the RIL population, 49 QTLs were identified that clustered in 30 genomic region. Twenty-three of these QTLs were confirmed in the SSSL population. A comparison with previously reported genes/QTLs revealed eight novel genomic regions that contained uncharacterized ORFs associated with leaf size. The QTLs identified in this study can be used for marker- assisted breeding and for fine mapping of novel genetic elements controlling leaf size in rice.

关键词： indica cross Genetic dissection of top three leaf traits in rice using progenies from a japonica RIL

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双撑杆智能预应力钢桁梁承载性能参数分析

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东南大学学报（自然科学版） 2016年第4期46卷 831-835页

作者：徐伟炜陈樑明东南大学混凝土及预应力混凝土结构教育部重点实验室南京210096 厦门合道工程设计集团有限公司厦门361004

运用ANSYS软件计算分析了双撑杆智能预应力钢桁梁跨中挠度约束范围、杆件应力约束范围、钢桁梁跨度、撑杆数量以及荷载形式5个因素对双撑杆智能预应力钢桁梁承载性能的影响,并将相同约束条件下双撑杆智能预应力钢桁梁与普通预应力钢桁... 详细信息

运用ANSYS软件计算分析了双撑杆智能预应力钢桁梁跨中挠度约束范围、杆件应力约束范围、钢桁梁跨度、撑杆数量以及荷载形式5个因素对双撑杆智能预应力钢桁梁承载性能的影响,并将相同约束条件下双撑杆智能预应力钢桁梁与普通预应力钢桁梁的承载性能进行了对比.结果表明:挠度约束范围越小、应力约束范围越宽、钢桁梁跨度越大,则智能预应力钢桁梁与普通预应力钢桁梁的最大约束承载力之比就越大;双撑杆智能预应力钢桁梁的承载性能优于单撑杆智能预应力钢桁梁;智能预应力钢桁梁在逐次增多荷载工况下的承载性能优于移动集中荷载工况下的承载性能.

关键词：钢桁梁智能预应力承载性能约束条件参数分析

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