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Endophytic bacteria in the periglacial plant Potentilla fruticosa var. albicans are influenced by habitat type

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Ecological Processes 2023年第4期12卷 149-163页

作者： Wangchen Sonam Yongqin Liu liangdong guo State Key Laboratory of Tibetan Plateau Earth System Environment and ResourcesTPESER Institute of Tibetan Plateau Research Chinese Academy of Sciences University of Chinese Academy of Sciences Center for the Pan-Third Pole Environment Lanzhou University State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences

Background Microbial communities in different plant compartments are relatively independent entities. However, the influence of environmental factors on the microbial community in different compartments of periglacial plants remains unclear. In this study, we quantified the bacterial communities in the rhizosphere soil, as well as root and leaf endosphere compartments of a periglacial plant, Potentilla fruticosa var. albicans, using high-throughput DNA sequencing. Moreover, we evaluated the impacts of habitat types(glacier terminus zone, moraine ridge, and alpine meadow) on the bacterial community in different plant compartments of Potentilla fruticosa var. *** Our results showed that habitat type had a significant effect on the alpha diversity(Chao1 richness) of endophytic bacteria, but not on the rhizospheric bacteria. The community composition of rhizospheric and endophytic bacteria was significantly different across the three habitats, and habitat type had a greater effect on the endophytic bacteria than on rhizospheric bacteria. The contribution of rhizosphere soil to the root and leaf endophytes decreased with the transformation of habitats from glacier terminus zone to alpine meadow. In contrast, host selection pressure sequentially increased from the glacier terminus zone to the moraine ridge to the alpine meadow. Furthermore, we found that the bacterial co-occurrence network in the alpine meadow was more modular but had lower complexity and connectedness than that in the glacier terminus zone. The bacterial community was governed primarily by stochastic processes regardless of habitat *** This study reveals that the diversity and composition of endophytic bacteria associated with Potentilla fruticosa var. albicans are more affected by habitat types than that of rhizospheric bacteria. Our study also demonstrates that the assembly patterns and co-occurrence patterns of bacterial communities associated with Potentilla fruticosa var. al

关键词： Bacterial community Endophytic bacteria Periglacial plant Potentilla fruticosa var. albicans

Changes in soil microbial community and enzyme activity along an exotic plant Eupatorium adenophorum invasion in a Chinese secondary forest

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Chinese Science Bulletin 2013年第33期58卷 4101-4108页

作者： SUN Xin GAO Cheng guo liangdong State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences

The exotic plant,Eupatorium adenophorum,has invaded rapidly across southwest China,damaging native ecosystems and causing great economic *** microbes,as an important component of belowground community,can drive nutrient cycling and regulate plant competition in terrestrial ***,knowledge of the soil microbial community and function will enhance our understanding of the mechanism of exotic plant invasive process in natural *** this study,we examined the soil microbial community,soil enzyme activity,soil property and plant community along an invasive gradient of *** in a southwest Chinese secondary *** soil analysis demonstrated that heavy invasion significantly increased the total P and NO3–-N contents,whereas it significantly decreased the total N and soil organic matter *** available P content was significantly decreased by moderate *** *** invasion significantly decreased the biomass of total soil microbes,as well as Gram-negative bacteria,actinomycetes,arbuscular mycorrhizal(AM)fungi and non-AM ***,*** invasion significantly increased the activities of soil urease,acid phosphatase,polyphenol oxidase and ***-metric multidimensional scaling showed that soil microbial composition and soil enzyme composition were significantly different in the three *** invaded *** Mantel tests indicated that plant composition was the most important factor for structuring soil microbial and enzyme *** results suggest that changes in soil microbial community structure and enzyme activity may play an important role in the process of *** invasion in a Chinese secondary forest ecosystem.

关键词：土壤微生物群落外来植物入侵中国西南地区土壤酶活性紫茎泽兰次生林陆地生态系统微生物群落结构

Tripodalsporormielones A-C,unprecedented cage-like polyketides with complex polyvdent bridged and fused ring systems

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Acta Pharmaceutica Sinica B 2021年第11期11卷 3648-3654页

作者： guodong Chen Bingxin Zhao Meijuan Huang Jia Tang Yanbing Li liangdong guo Rongrong He Dan Hu Xinsheng Yao Hao Gao Institute of Traditional Chinese Medicine&Natural Products College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs ResearchJinan UniversityGuangzhou 510632China Department of Chemistry College of Chemistry and Materials ScienceJinan UniversityGuangzhou 510632China Guangdong Engineering Research Center of Chinese Medicine&Disease Susceptibility Jinan UniversityGuangzhou 510632China State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100190China

A chemical investigation on Sporormiella *** to the isolation and structural elucidation of tripodalsporormielones Ae C(1-3),a new class of polyketide possessing unprecedented cage-like skeletons with polyvdent bridged and fused ring *** polyketides with cage-like skeletons were characterized as a high non-protonated carbon-containing system,which resulted in few HMBC correlations observed and made the accurate structures hard to be obtained by ***,some signals of non-protonated sp;carbons are weak and even unobservable in compound *** order to establish the structure of 1,the calculated NMR with DP4 evaluation was applied to determine the structure from the plausible structure candidates obtained from the detailed NMR *** on NMR experiments and calculated NMR,the structures of isolated compounds were established and confirmed by X-ray *** chiral isolation,the optically pure enantiomers of 1 and 3 were obtained,and their absolute configurations were determined based on ECD quantum chemical *** on the isolated compounds and our previous work,1-3 would be derived from 3-methylorcinaldehyde,and their plausible biosynthetic mechanism was ***,1 exhibited obvious short-term memory improvement activity on an Alzheimer’s disease fly model.

关键词： Tripodalsporormielones Cage-like polyketides 3-Methylorcinaldehyde Alzheimer’s disease

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真菌群落构建机制研究进展

菌物学报 2023年第1期42卷 13-25页

作者：李洁李杏春郭良栋中国科学院微生物研究所真菌学国家重点实验室北京100101 中国科学院大学生命科学学院北京100049

真菌是真核生物中生物多样性最丰富的类群之一,广泛分布于自然界,在生物多样性的分布与维持、群落构建以及物质与能量循环过程中发挥着重要的生态功能。近年来随着DNA高通量测序技术和生物信息学的发展,极大推动了真菌多样性分布格局与... 详细信息

真菌是真核生物中生物多样性最丰富的类群之一,广泛分布于自然界,在生物多样性的分布与维持、群落构建以及物质与能量循环过程中发挥着重要的生态功能。近年来随着DNA高通量测序技术和生物信息学的发展,极大推动了真菌多样性分布格局与群落构建机制的研究进展。本文概述了基于生态位理论(niche theory)和中性理论(neutral theory)的选择(selection)、扩散(dispersal)、成种(speciation)和漂变(drift)等过程与生态模型的定量分析确定性过程和随机性过程对群落构建贡献的机制,及其在真菌群落构建中应用的最新研究进展。

关键词：真菌群落构建确定性过程随机性过程生态模型

Dimericbiscognienynes B and C: New diisoprenyl-cyclohexene-type meroterpenoid dimers from Biscogniauxia sp.

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Chinese Chemical Letters 2019年第1期30卷 51-54页

作者： Huan Zhao Meizhi Wang guodong Chen Dan Hu Enqing Li Yibo Qu Libing Zhou liangdong guo Xinsheng Yao Hao Gao Institute of Traditional Chinese Medicine and Natural Products College of Pharmacy / Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research Jinan University College of Traditional Chinese Medicine Jinan University Guangdong-Hongkong-Macao Institute of CNS Regeneration Joint International Research Laboratory of CNS Regeneration Jinan University State Key Laboratory of Mycology Institute of Microbiology Chinese Academy of Sciences

Dimericbiscognienynes B and C(1 and 2), two new diisoprenyl-cyclohexene-type meroterpenoid dimers,were isolated from Biscogniauxia sp. 71-10-1-1. Their structures, including the absolute configurations,were determined by spectroscopic analyses and ECD experiments. Meroterpenoids are special natural products that originate from mixed terpenoid-nonterpenoid pathway. As a member of meroterpenoid family, diisoprenyl-cyclohexene/ane-type meroterpenoids composed of two isoprenyl chains(C5 unit)and a cyclohexene/ane moiety(C6 unit), featuring diverse skeleton structures with wide range of bioactivities. In these reported diisoprenyl-cyclohexene/ane-type meroterpenoids, only three dimers were identified. The discovery of the two new dimers added members of this rare class of meroterpenoids.

关键词： Meroterpenoid Dimer Diisoprenyl-cyclohexene/ane-type meroterpenoid Diels-Alder reaction Biscogniauxia sp.

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基于支持向量回归模型的焦炭质量智能分析

燃料与化工 2023年第2期54卷 11-14,20页

作者：郭亮东国家能源集团煤焦化有限责任公司乌海016000

炼焦配煤是焦化企业生产过程中的一项关键工作。在钢铁工业中,焦炭在高温下的CSR和CRI质量指标尤其重要。为了改善需依赖经验的传统配煤手段,基于原料煤指标预测焦炭质量的思路被提出。通过分析国内外的一些预测方法,针对目前存在的不... 详细信息

炼焦配煤是焦化企业生产过程中的一项关键工作。在钢铁工业中,焦炭在高温下的CSR和CRI质量指标尤其重要。为了改善需依赖经验的传统配煤手段,基于原料煤指标预测焦炭质量的思路被提出。通过分析国内外的一些预测方法,针对目前存在的不足进行改良,以西来峰焦化厂一期和二期为例,采用支持向量回归模型,对焦炭的CSR和CRI这2个质量指标进行了智能分析,并通过系统分析训练样本比例与预测精度的关系,指明进一步提升焦炭质量预测性能的工作方向。

关键词：支持向量回归模型 CSR CRI

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配煤炼焦生产在线管理平台的应用

燃料与化工 2023年第3期54卷 35-38页

作者：郭亮东国家能源集团煤焦化有限责任公司乌海016000

通过建设和应用配煤炼焦生产在线管理平台,将配煤计算、铁箱/小焦炉试验和实际炼焦生产紧密结合,规范了配煤方案审批过程,对实现科学配煤、保证焦炭质量、降低配煤成本起到了重要的作用。

关键词：配煤炼焦在线管理平台

New delay-decomposing method for stability analysis of genetic regulatory networks with time-varying delays

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New delay-decomposing method for stability analysis of genet...

第36届中国控制会议

作者： Jin Zhang Jinhai Zhang liangdong guo University of Science and Technology Liaoning

ISBN: (纸本)9781538629185

The problem of the stability analysis of genetic regulatory networks（GRNs） with time varying delays are proposed in this paper based on a new delay-decomposing *** appropriate Lyapunov-krasovskiis functionals are defined on each of the obtained subintervals,some integral inequalities and the convex combination technique are utilized to deal with the bound of the derivative of the Lyapunov-krasovskiis *** the criteria are derived in terms of linear matrix inequalities（LMIs）.Finally,a numerical example is used to illustrate the usefulness and less conservative than relative references.

关键词： Genetic Regulatory Networks Stability Time-varying delay Delay-decomposing method

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中国微生物物种多样性研究进展

生物多样性 2012年第5期20卷 572-580页

作者：郭良栋中国科学院微生物研究所真菌学国家重点实验室北京100101

微生物是分布最为广泛的生命形式,几乎分布到地球上的所有生境,具有丰富的物种多样性。我国地域辽阔,跨越热带至寒温带,气候条件多样,地理环境与生态系统类型复杂,是世界上生物多样性最丰富的国家之一。我国已开展了大量微生物多样性研... 详细信息

微生物是分布最为广泛的生命形式,几乎分布到地球上的所有生境,具有丰富的物种多样性。我国地域辽阔,跨越热带至寒温带,气候条件多样,地理环境与生态系统类型复杂,是世界上生物多样性最丰富的国家之一。我国已开展了大量微生物多样性研究,并证实我国多样的生境蕴藏着丰富的微生物物种多样性。目前我国已报道真核微生物(菌物)约14,700种,其中包括真菌约14,060种、卵菌约300种、黏菌约340种,而真菌中有药用菌473种、食用菌966个分类单元。特别是近年来通过免培养的分子生物学技术发现我国存在丰富的原核微生物多样性。本文概述了传统方法和现代分子生物学技术在我国原核微生物(古菌、细菌)和真核微生物(真菌、卵菌、黏菌)物种多样性研究的最新进展。

关键词：真核微生物原核微生物物种多样性培养方法分子技术