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OsRLCK160 contributes to flavonoid accumulation and UV-B tolerance by regulating OsbZIP48 in rice

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Science China(Life Sciences) 2022年第7期65卷 1380-1394页

作者： Feng Zhang Jiacheng Huang Hao Guo Chenkun Yang Yufei Li Shuangqian Shen Chuansong Zhan lianghuan qu Xianqing Liu Shouchuang Wang Wei Chen Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China College of Tropical Crops Hainan UniversityHaikouHainan 570288China

Plants produce specialized metabolites to adapt to the ever-changing *** are antioxidants essential for growth,development,and breeding with increased stress resistance in ***,the mechanism of the involvement of flavonoids in ultraviolet-B(UV-B)stress in rice(Oryza sativa)is largely *** this study,we cloned and functionally identified a receptor-like kinase(OsRLCK160)and a bZIP transcription factor(OsbZIP48)positively regulating flavonoid accumulation through metabolite-based genome-wide association study of the flavonoid content in ***,OsRLCK160 interacted with and phosphorylated OsbZIP48 to regulate the flavonoid accumulation and participate in UV-B tolerance in *** study indicates the importance of applying OsRLCK160 and OsbZIP48 to advance the fundamental understanding of stable rice production and breed UV-B-tolerant rice varieties,which may contribute to breeding high-yield rice varieties.

关键词： flavonoids natural variation Oryza sativa receptor-like kinase transcription factor UV-B tolerance

Branched-chain amino acids regulate plant growth by affecting the homeostasis of mineral elements in rice

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Science China(Life Sciences) 2019年第8期62卷 1107-1110页

作者： Cheng Jin Yangyang Sun Yuheng Shi Yuanyuan Zhang Kai Chen Yuan Li Guige Liu Fang Yao Deng Cheng Jie Li Junjie Zhou lianghuan qu Xianqing Liu Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong AgriculturalUniversityWuhan430070China Department of Metabolic Biology John Innes CentreNorwich Research ParkNorwich NR47UHUK Institute of Tropical Agriculture and Forestry of Hainan University Haikou570288China

Dear Editor,Branched-chain amino acids(BCAAs)are essential amino acids that must be obtained from the diet for humans and ***-chain amino acid transaminases(BCATs)catalyze a key step in the BCAA synthesis pathway,however,little is known about their biological roles in *** this study,we functionally characterized two BCATs,OsBCAT4 and OsBCAT5,in rice and showed that they regulate the development of rice roots by affecting the levels of mineral elements.

关键词： Sr Branched-chain amino acids regulate plant growth by affecting the homeostasis of mineral elements in rice

Control of Leaf Senescence by an MeOH- Jasmonates Cascade that Is Epigenetically Regulated by OsSRT1 in Rice

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Molecular Plant 2016年第10期9卷 1366-1378页

作者： ChuanYing Fang Hua Zhang Jian Wan YangYang Wu Kang Li Cheng Jin Wei Chen ShouChuang Wang WenSheng Wang HaiWei Zhang Pan Zhang Fei Zhang lianghuan qu Xianqing Liu Dao-Xiu Zhou Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070China Institute for Nutritional Sciences Shanghai Institutes for Biological Sciences Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai200031 China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China Institute of Plant Sciences Paris-Saclay Universite Paris-sud 11 Orsay 91405 France

Although considerable progress has been made in identifying the genes regulating accumulation of hormones that are involved in leaf senescence, only a few studies have focused on natural variations in jasmonates content and much less on the underlying genetic basis. Moreover, the epigenetic regulation of jasmonate-mediated leaf senescence remains largely unknown, in this study, we carried out metabolic profiling of a worldwide collection of rice accessions and demonstrated that there are substantial variations in jasmonate levels among these accessions. A subsequent metabolite-based genornewide association study identified candidates for two major quantitative genes （QTGs）, OsPME1 and OsTSD2, affecting the content of jasmonates. Further investigations using a series of relevant mutants and transgenic lines revealed the MeOH-jasmonata cascade plays an important role in regulating leaf senescence. Moreover, we showed that OsSRT1, one of the two Sir2 （silent information regugator 2） homologs in rice, negatively regulates leaf senescence by repressing expression of the biosynthetic genes of this metabolic cascade and at least particiaUy through histone H3K9 deacetylation of OsPME1. Taken together, our results indicate that the MeOH-jasmonates cascade and its epigenetic regulation are crucial for controlling leaf senescence process in rice.

关键词： MeOH-jasmonate cascade leaf senescence natural variation epigenetic regulation

Rice metabolic regulatory network spanning the entire life cycle

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Molecular Plant 2022年第2期15卷 258-275页

作者： Chenkun Yang Shuangqian Shen Shen Zhou Yufei Li Yuyuan Mao Junjie Zhou Yuheng Shi Longxu An Qianqian Zhou Wenju Peng Yuanyuan Lyu Xuemei Liu Wei Chen Shouchuang Wang lianghuan qu Xianqing Liu Alisdair R.Fernie Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070China College of Tropical Crops Hainan UniversityHaikou 570228China Sanya Nanfan Research Institute of Hainan University Hainan Yazhou Bay Seed LaboratorySanya 572025China College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Max Planck Institute of Molecular Plant Physiology Potsdam-Golm 14476Germany Centre of Plant Systems Biology and Biotechnology Plovdiv 4000Bulgaria

As one of the most important crops in the world,rice(Oryza sativa)is a model plant for metabolome *** many studies have focused on the analysis of specific tissues,the changes in metab-olite abundance across the entire life cycle have not yet been *** this study,combining both tar-geted and nontargeted metabolite profiling methods,a total of 825 annotated metabolites were quantified in rice samples from different tissues covering the entire life *** contents of metabolites in different tissues of rice were significantly different,with various metabolites accumulating in the plumule and radicle during seed *** these data with transcriptome data obtained from the same time period,we constructed the Rice Metabolic Regulation *** metabolites and co-expressed genes were further divided into 12 clusters according to their accumulation patterns,with members within each cluster displaying a uniform and clear pattern of abundance across *** this dataset,we established a comprehensive metabolic profile of the rice life cycle and used two independent strategies to identify novel transcription factors-namely the use of known regulatory genes as bait to screen for new networks underlying lignin metabolism and the unbiased identification of new glycerophospholipid metabolism regulators on the basis of tissue *** study thus demonstrates how guilt-by-association analysis of metabolome and transcriptome data spanning the entire life cycle in cereal crops provides novel resources and tools to aid in understanding the mechanisms underlying important agro-nomic traits.

关键词： rice metabolome transcriptome transcription factor co-expression glycerophospholipid

水稻果胶甲基转移酶OsTSD2基因突变体的分析

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华中农业大学学报 2017年第3期36卷 25-31页

作者：张飞曲良焕罗杰华中农业大学作物遗传改良国家重点实验室武汉430070 淮北师范大学生命科学学院淮北235000

探讨水稻果胶甲基转移酶OsTSD2基因的功能,尤其是对生长发育的影响,筛选并鉴定了OsTSD2基因突变的3个突变体tsd2a、tsd2b和tsd2c。表型观察显示:突变体水稻结实率下降、种子粒长变短、粒宽变窄、千粒重下降;突变体的颖壳颜色变暗,颖壳... 详细信息

探讨水稻果胶甲基转移酶OsTSD2基因的功能,尤其是对生长发育的影响,筛选并鉴定了OsTSD2基因突变的3个突变体tsd2a、tsd2b和tsd2c。表型观察显示:突变体水稻结实率下降、种子粒长变短、粒宽变窄、千粒重下降;突变体的颖壳颜色变暗,颖壳的破裂程度加重并出现新的背部破裂;荧光免疫标记试验研究发现,突变体种子萌发率下降可能是由于突变体种子盾片中的甲基化果胶含量低所致。结果表明,OsTSD2基因的下调导致水稻的种子在发育和萌发过程中存在明显的缺陷。

关键词：水稻果胶甲基转移酶 OsTSD2 种子萌发

真空预冷压强对鲜枸杞SOD酶活性的影响及分子动力学模拟

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食品工业科技 2025年第1期46卷 54-60页

作者：张宏博康宁波周良欢张军瞿前进宁夏大学食品科学与工程学院宁夏银川750021

为探究真空预冷处理对鲜枸杞超氧化物歧化酶(superoxide dismutase,SOD)活性的影响及其分子水平机理,本研究将宁夏新鲜枸杞置于真空预冷900、1100 Pa压强下处理,提取粗酶液进行SOD酶活性测定,并进行150 ns分子动力学模拟研究SOD酶在不... 详细信息

为探究真空预冷处理对鲜枸杞超氧化物歧化酶(superoxide dismutase,SOD)活性的影响及其分子水平机理,本研究将宁夏新鲜枸杞置于真空预冷900、1100 Pa压强下处理,提取粗酶液进行SOD酶活性测定,并进行150 ns分子动力学模拟研究SOD酶在不同真空预冷压强处理下的内部变化,分析均方根误差、回旋半径、总能量、势能、动能、键角能、均方根涨落、氢键数量、表面结构、溶剂可及表面积的改变,进而筛选出真空预冷处理的最佳压强条件。结果显示,900 Pa处理下SOD酶活最高。分子动力学模拟结果表明,体系总能量、势能、动能及键角能均处于平衡稳定状态,压强增大使得蛋白质表面结构改变,氢键数量减少,溶剂可及表面积不断减小。在900 Pa处理下,蛋白质结构最紧密,活性口袋变宽且易与蛋白结合。而1100 Pa处理下酶蛋白活性降低,蛋白结构破坏,活性口袋变小。本研究可为研究鲜枸杞保鲜及加工提供科学参考。

关键词：枸杞真空预冷超氧化物歧化酶分子动力学模拟

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基于知识框架构建的《分子生物学》翻转课堂改革与实践

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生命的化学 2019年第4期39卷 830-834页

作者：曲良焕郑用琏彭楠王海娇练兴明罗杰金安江华中农业大学生命科学技术学院

由知识传授向能力培养转变是大学课堂教学改革始终要坚持的方向。借助于优质的数字课程资源,课程组在华中农业大学国家生物学理科基地班开展《分子生物学》理论教学的翻转课堂实践。课程围绕知识框架的构建与完善,充分发挥教师的引导作... 详细信息

由知识传授向能力培养转变是大学课堂教学改革始终要坚持的方向。借助于优质的数字课程资源,课程组在华中农业大学国家生物学理科基地班开展《分子生物学》理论教学的翻转课堂实践。课程围绕知识框架的构建与完善,充分发挥教师的引导作用,从用好自学平台、精心组织见面课、鼓励拓展学习等方面开展改革探索。实践表明,该模式有助于激发学生的学习兴趣,促进师生间、学生间的交流与思想碰撞,能有效培养学生自主学习能力和科学思维,达到深度学习的效果。

关键词：《分子生物学》翻转课堂知识框架构建深度学习

基于浮选法的鼠笼式鲜枸杞分级机的设计与试验

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食品与机械 2024年第10期40卷 86-92页

作者：瞿前进张军康宁波邓改革周良欢张宏博宁夏大学食品科学与工程学院宁夏银川750021 宁夏大学土木与水利工程学院宁夏银川750021

[目的]提高鲜枸杞分级效率。[方法]设计基于浮选法的鼠笼式鲜枸杞分级机,以鲜枸杞横径为分级指标。利用鲜枸杞密度小于水的物理特性,运用浮选原理研制鲜枸杞分级机。通过力学分析及试验测量,计算和规定了分级机的理论结构参数。基于ANSY... 详细信息

[目的]提高鲜枸杞分级效率。[方法]设计基于浮选法的鼠笼式鲜枸杞分级机,以鲜枸杞横径为分级指标。利用鲜枸杞密度小于水的物理特性,运用浮选原理研制鲜枸杞分级机。通过力学分析及试验测量,计算和规定了分级机的理论结构参数。基于ANSYS Fluent模拟分级过程,分析模拟结果,并验证和精确该分级机的理论结构参数。[结果]分级机具体参数为:鼠笼直径0.34 m,长度2.1 m,辊杠直径10 mm,鼠笼倾斜角度17°,水流量1.7×10-2 m3/s,水流速3.5 m/s,鼠笼转速12 r/min。平行试验结果表明,鲜枸杞平均分级准确率约为93.79%,鲜枸杞无损率为98.21%;批量试验表明,鲜枸杞平均生产能力为1020.6 kg/h,平均分级准确率为92.85%。[结论]试验研制的基于浮选法的鼠笼式鲜枸杞分级机能够实现鲜枸杞低损、快速、高效分级。

关键词：浮选法鼠笼鲜枸杞横径

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