检索结果-南通市图书馆

Switching On/Off of Guide RNA by Photoinduced Strand Displacement for Functional Control of CRISPR/Cas9

CCS Chemistry 2024年第5期6卷 1338-1351页

作者： Yang Wang Chao-Qun wu Qiu-Long Zhang Ming Shao Yan Liu liang-liang Wang Zun-Yun Wang Juan Du liang Xu Central Laboratory Department of the Second Affiliated Hospital School of MedicineThe Chinese University of Hong KongShenzhen&Longgang District People’s Hospital of ShenzhenShenzhen 518172 Guangdong Key Laboratory for biomedical Measurements and Ultrasound Imaging National-Regional Key Technology Engineering Laboratory for Medical UltrasoundSchool of Biomedical EngineeringShenzhen University Medical SchoolShenzhen 518060 MOE Key Laboratory of bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-sen UniversityGuangzhou 510275 School of Pharmacy and Medical Technology Putian UniversityPutian 351100

Functional control of CRISPR/Cas9 is essential for precise gene *** engineering of guide RNA(gRNA)provides diverse approaches for conditional control of CRISPR/Cas9 function with a variety of chemical reactive ***,previous investigations into chemically engineering gRNA only unidirectionally regulated the CRISPR/Cas9 function via stimuli-induced caging/decaging ***,we propose a combinatory strategy to engineer the dynamics of gRNA in which photocontrolled strand-displacement reactions coupled with sequence designs of gRNA can achieve lightinduced switching-on/off control of CRISPR/Cas9 *** analysis and cellular gene regulation indicate this approach is capable of both activating and deactivating CRISPR/Cas9 activities using light ***,photocontrolled multiplex modulations of gene expression for opposite regulatory effects have also been achieved simultaneously under the same cellular *** work establishes an essential principle for construction of stimuli-induced switching-on/off modulations of gRNA that can greatly enrich the versatility of conditional control for a variety of CRISPR/Cas9-based applications.

关键词： modified oligonucleotides gene manipulation optical control CRISPR/Cas9 strand displacement

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

龙脑樟去油枝叶中总多酚的酶解-超声辅助提取及抗氧化活性

引用

天然产物研究与开发 2018年第6期30卷 1037-1045页

作者：张峰毕良武赵振东王鹏中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室南京210042 中国林业科学研究院林业新技术研究所北京100091 江西农业大学江西特色林木资源培育与利用2011协同创新中心南昌330045

为探讨龙脑樟去油枝叶中总多酚的酶解-超声辅助提取工艺,在单因素实验的基础上,通过Box-Behnken设计-响应面法研究纤维素酶用量(X_1)、乙醇浓度(X_2)、液料比(X_3)对龙脑樟去油枝叶中总多酚提取率的影响;同时,以清除DPPH自由基和羟基自... 详细信息

为探讨龙脑樟去油枝叶中总多酚的酶解-超声辅助提取工艺,在单因素实验的基础上,通过Box-Behnken设计-响应面法研究纤维素酶用量(X_1)、乙醇浓度(X_2)、液料比(X_3)对龙脑樟去油枝叶中总多酚提取率的影响;同时,以清除DPPH自由基和羟基自由基的能力为评价指标,对总多酚提取物的抗氧化活性进行测定。结果表明,各因素对总多酚提取率的影响顺序为液料比(X_3)>乙醇浓度(X_2)>酶用量(X_1);酶解-超声辅助提取龙脑樟去油枝叶中总多酚的最优工艺条件为:酶用量1.9%,乙醇浓度54%,液料比34 m L/g,酶解温度50℃,酶解时间1.5 h,超声时间30 min,超声频率45 k Hz,超声功率360 W,超声温度70℃;在此条件下,总多酚提取率的平均值为22.08 mg/g,与预测值相近,二次回归模型预测性良好。总多酚提取物对DPPH自由基和羟基自由基的清除作用均呈现量效关系,半数抑制浓度(IC_(50))值分别为0.36和0.15 mg/m L,表明总多酚提取物具有较强的抗氧化能力。

关键词：响应面法龙脑樟去油枝叶酶解-超声辅助提取总多酚抗氧化活性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

大孔树脂富集迷迭香酸的静态吸附和解析研究

引用

天然产物研究与开发 2016年第4期28卷 601-606页

作者：程贤毕良武赵振东张峰中国林业科学研究院林产化学工业研究所生物质化学利用国家工程实验室国家林业局林产化学工程重点开放性实验室江苏省生物质能源与材料重点实验室南京210042 中国林业科学研究院林业新技术研究所北京100091 江西农业大学江西特色林木资源培育与利用2011协同创新中心南昌330045

考察了AB-8、D101、X-5、NKA-II 4种大孔吸附树脂对迷迭香叶中迷迭香酸的静态吸附和解析性能。以吸附量、吸附率、解吸量、解析率为指标,筛选出较好的AB-8树脂。通过静态吸附和解析实验,研究了吸附时间和温度对AB-8静态吸附过程的影响,... 详细信息

考察了AB-8、D101、X-5、NKA-II 4种大孔吸附树脂对迷迭香叶中迷迭香酸的静态吸附和解析性能。以吸附量、吸附率、解吸量、解析率为指标,筛选出较好的AB-8树脂。通过静态吸附和解析实验,研究了吸附时间和温度对AB-8静态吸附过程的影响,同时研究了一定温度和解析液中乙醇浓度对迷迭香酸静态解析过程的影响,并且从热动力学角度研究分析其吸附过程。结果表明,准二级反应动态模型、Langmuir方程和Freundlich方程适用于静态吸附过程,乙醇浓度是影响解析率的关键因素。

关键词：迷迭香酸大孔吸附树脂静态吸附静态解析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A portable,auxiliary photovoltaic power system for electric vehicles based on a foldable scissors mechanism

Energy and Built Environment

引用

Energy and Built Environment 2024年第1期5卷 81-96页

作者： Zhou Jin Dongyang Li Daning Hao Zutao Zhang liang Guo Xiaoping wu Yanping Yuan China Railway wuhan Electrification Bureau Group Shanghai Electric Co. LtdShanghai 201799PR China School of Mechanical Engineering Southwest Jiaotong UniversityChengdu 610031PR China

In recent years,countries worldwide have actively advocated electric vehicles for environmental ***-ever,restrictions on the driving range and charging have hampered the promotion of electric *** study proposes a portable,auxiliary photovoltaic power system based on a foldable scissors mechanism for electric *** system includes a photovoltaic power generation module and an electricity transfer *** photovoltaic power generation module built based on a foldable scissors mechanism is five times smaller than in its unfolded state,improving its portability in its folded *** electricity transfer module transfers electricity into the cabin via wireless power transfer units and stores electricity in *** simulation exper-iments were conducted to evaluate the system’s performance:maximum output power of 1.736 W is measured when the load is 5Ω,while maximum wireless power transfer efficiency is up to 57.7% with 10Ω *** elec-tric vehicle in Chengdu city was simulated for a case *** results show that the annual output of a single photovoltaic power system can drive the MINIEV for 423.625 km,indicating that the proposed system would be able to supply power for electric vehicles as an auxiliary power supply system.

关键词： Auxiliary photovoltaic power system Energy harvesting Scissors mechanism Solar collector Electric vehicle

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A nonradical oxidation process initiated by Ti-peroxo complex showed high specificity toward the degradation of tetracycline antibiotics

引用

Chinese Chemical Letters 2024年第5期35卷 366-370页

作者： Jian Peng Yue Jiang Shuangyu wu Yanran Cheng Jingyu liang Yixin Wang Zhuo Li Sijie Lin College of Environmental Science and Engineering Biomedical Multidisciplinary Innovation Research InstituteShanghai East HospitalTongji UniversityShanghai 200092China Key Laboratory of Yangtze River Water Environment Shanghai Institute of Pollution Control and Ecological SecurityTongji UniversityShanghai 200092China

Nonradical oxidation has received wide attention in advanced oxidation processes for environmental *** the relationship between material characteristics and their ability to initiate nonradical oxidation processes is the key to better material design and ***,a novel titanium-based metal-organic framework MIL-125-Ti/H_(2)O_(2) system was established to show a highly selective degradation efficacy toward tetracycline ***-125-Ti with the abundance of TiO6 octahedra units was found to effectively activate H_(2)O_(2) under dark conditions by forming an oxidative Ti-peroxo *** presence of the Ti-peroxo complex,confirmed by UV-visible spectrophotometer,fourier transform infrared spectroscopy,and X-ray photoelectron spectroscopy characterizations,showed superior degradation(>95%removal rate)of oxytetracycline hydrochloride(OTC),doxycycline hydrochloride,chlortetracycline hydrochloride,and *** functional theory calculations were performed to assist the elucidation on the mechanism of H_(2)O_(2) activation and antibiotics *** MIL-125-Ti/H_(2)O_(2) system was highly resistant to halogens and background organics,and could well maintain its original catalytic activity in actual water *** retained the ability to degrade 75%of OTC within ten test *** study provides new insight into the nonradical oxidation process initiated by the unique Ti-peroxo complex of Ti-based MOF.

关键词： Nonradical oxidation Metal-organic frameworks Advanced oxidation process Ti-peroxo complex Antibiotics degradation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Exploring centimeter-sized crystals of bismuth-iodide perovskite toward highly sensitive X-ray detection

引用

Chinese Chemical Letters 2024年第6期35卷 185-188页

作者： Xin Dong Jing liang Zhijin Xu Huajie wu Lei Wang Shihai You Junhua Luo Lina Li College of Chemistry Fuzhou UniversityFuzhou 350116China State Key Laboratory of Structure Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China University of Chinese Academy of Sciences Beijing 100049China

Lead-halide perovskites exhibit outstanding performance in X-ray detection due to their intrinsic features such as high charge carrier mobility,large atomic number,and long carrier lifetime,but the toxicity of lead is regarded as the major factor hindering their ***,we introduce organic molecule(R)-(-)-2-methylpiperazine(R-MPz)into the bismuth-based structure to synthesize lead-free(R)-(H_(2)MPz)biI_(5)(R-Mbi).The high-quality centimeter-sized single crystals have been obtained,which show a low dark current and superior environmental ***,the single-crystal device of R-Mbi exhibits a highμτproduct up to 1.88×10^(-4)cm^(2)/V and a low trap density of 1.21×10^(10)cm^(-3).Further,the detector displays excellent detection sensitivity of 263.58μC Gy_(air)^(-1)cm^(-2)and a favorable low detection limit of 4.35μGyair/s,both of which meet the requirement for medical *** findings shed light on the exploration of innovative bismuth-based hybrid perovskites for high-performance X-ray detection.

关键词： Perovskite Lead-free bismuth-based X-ray detection Sensitivity

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Deriving skeletal muscle cells from adipose-derived stem cells:Current differentiation strategies

引用

Chinese Medical Journal 2024年第12期137卷 1498-1500页

作者： Wei liang Meng Han Huiting wu Wanwen Dang Xiaoyu Meng Yonghuan Zhen Yang An Department of Plastic Surgery Peking University Third HospitalBeijing 100191China

To the Editor:Adipose-derived stem cells(ASCs)are promising multipotent stem cells for regeneration of skeletal muscle(SM)tissues.[1]However,the low myogenic differentiation ability limits the application.[2]Therefore,it is necessary to summarize the current differentiation strategy to improve the differentiation *** methods(biochemical induction)and non-traditional strategies(co-culture,biomechanical approaches,and biomaterial utilization)are commonly used for the differentiation of ASCs into myogenic *** both strategies can effectively induce differentiation of myogenic cells,cellular unhealthy or immature myogenic differentiation status is still the greatest challenge for ***,the combination of non-traditional methods and traditional methods has the potential to significantly enhance myogenesis of ASCs with remarkable efficacy,however,evidence is currrently *** review discusses the traditional and non-traditional strategies,as well as several combination motheds of above strategies,for inducing ASCs differentiation into myogenic cells[Figure 1].

关键词： skeletal Figure strategies

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A Fluorine-Functionalized 3D Covalent Organic Framework with Entangled 2D Layers

引用

Chinese Journal of Polymer Science 2024年第8期42卷 1210-1216页

作者： Li-Bang Xiao Zi-Han wu Jun-Jie Xin Yuan-Peng Cheng Bo Gui Jun-liang Sun Cheng Wang College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China College of Chemistry and Molecular Engineering Beijing National Laboratory for Molecular SciencesPeking UniversityBeijing 100871China

Constructing three dimensional(3D)covalent organic frameworks(COFs)through the entanglement of two dimensional(2D)nets is a promising but underdeveloped ***,we report the design and synthesis of a fluorine functionalized 3DCOF(3D-An-COF-F)formed by entangled 2D sql *** structure of 3D-An-COF-F was determined by the combination of continuous rotation electron diffraction technique and modelling based on the chemical information from real ***,compared to the isostructural 3D-An-COF without F atom s,3DAn-COF-F showed an improved CO_(2)sorption ability and higher CO_(2)/N_(2)*** study not only demonstrated the generality of constructing 3D COFs with entangled 2D nets by introducing bulky groups vertically in planar building blocks,but also will expand the diversity of 3D COFs for various applications.

关键词： 3D covalent organic frameworks Fluorine Entangled 2D layers Electron diffraction CO_(2)adsorption

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Universal architecture and defect engineering dual strategy for hierarchical antimony phosphate composite toward fast and durable sodium storage

引用

Journal of Energy Chemistry 2024年第3期90卷 110-119,I0004页

作者： Jiawei wu Gaoyu Wang Wei Zhang Lixiang Wang Jian Peng Qinghua Li Zhixin liang Wenbo Fan Jiazhao Wang Shaoming Huang School of Materials and Energy Guangzhou Key Laboratory of Low-Dimensional Materials and Energy Storage DevicesGuangdong University of TechnologyGuangzhou 510006GuangdongChina School of Mechanical and Electrical Engineering Jiaxing Nanhu UniversityJiaxing 314001ZhejiangChina Institute for Superconducting and Electronic Materials Australian Institute for Innovative MaterialsUniversity of WollongongInnovation CampusSquires WayNorth WollongongNSW2522Australia

Antimony(Sb)-ba sed anode materials are feasible candidates for sodium-ion batteries(SIBs) due to their high theoretical specific capacity and excellent electrical ***,they still suffer from volume distortion,structural collapse,and ionic conduction interruption upon ***,a hierarchical array-like nanofiber structure was designed to address these limitations by combining architecture engineering and anion tuning strategy,in which SbPO_(4-x) with oxygen vacancy nanosheet arrays are anchored on the surface of interwoven carbon nanofibers(SbPO_(4-x)@CNFs).In particular,bulky PO_(4)^(3-) anions mitigate the large volume distortion and generate Na_(3)PO_(4) with high ionic conductivity,collectively improving cyclic stability and ionic transport *** abundant oxygen vacancies substantially boost the intrinsic electronic conductivity of SbPO_4,further accelerating the reaction *** addition,hierarchical fibrous structures provide abundant active sites,construct efficient conducting networks,and enhance the electron/ion transport *** from the advanced structural design,the SbPO_(4-x)@CNFs electrodes exhibit outstanding cycling stability(1000 cycles at 1.0 A g^(-1) with capacity decay of 0.05% per cycle) and rapid sodium storage performance(293.8 mA h g^(-1) at 5.0 A g^(-1)).Importantly,systematic in-/ex-situ techniques have revealed the "multi-step conversion-alloying" reaction process and the "battery-capacitor dual-mode" sodium-storage *** work provides valuable insights into the design of anode materials for advanced SIBs with elevated stability and superior rate performance.

关键词： SbPO_(4) anodes Hierarchical nanostructures Oxygen vacancy Stress dispersion Sodium-ion batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molten metal-organic complex to synthesize versatile ultrathin nonlayered oxides

引用

Nano Research 2024年第4期17卷 3147-3155页

作者： Kaisi Liu Jiabin wu Qun Li Hongrun Jin Yongxin Luo Bei Qi Simin Dai Jianquan Zhao liang Huang Jun Zhou Hubei Key Laboratory of biomass Fibers and Eco-Dyeing&Finishing Wuhan Textile UniversityWuhan 430200China wuhan National Laboratory for Optoelectronics School of Optical and Electronic InformationHuazhong University of Science and TechnologyWuhan 430074China Analytical and Testing Center Huazhong University of Science and TechnologyWuhan 430074China

Two-dimensional(2D)materials have attracted a great deal of research interest because of their unique electrical,magnetic,optical,mechanical,and catalytic properties for various *** date,however,it is still difficult to fabricate most functional oxides as 2D materials unless they have a layered ***,we report a one-step universal strategy for preparing versatile non-layered oxide nanosheets by directly annealing the mixture of metal nitrate and dimethyl imidazole(2-MI).The 2-MI plays the key role for 2D oxides since 2-MI owns a very low molten point and sublimation temperature,in which its molten liquid can coordinate with metal ions,forming a metal-organic framework,and easily puffing by its gas molecules.A total of 17 materials were prepared by this strategy,including non-layered metal oxide nanosheets as well as metal/metal oxide loaded nitrogen-doped carbon *** as-prepared cobalt particle-loaded nitrogen-doped carbon nanosheets(Co@N/C)exhibit remarkable bifunctional oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)electrocatalytic activity and ***,the Zn-air battery utilizing a Co@N/C catalyst exhibits high power density of 174.3 mW·cm^(-2).This facile strategy opens up a new way for large-scale synthesis of 2D oxides that holds great potential to push 2D oxides for practical applications.

关键词： non-layered oxide nanosheet metal/metal oxide@N/C oxygen evolution reaction(OER) oxygen reduction reaction(ORR) Zn-air battery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：