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Effect of fragile histidine triad gene transduction on proliferation and apoptosis of human hepatocellular carcinoma cells

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World Journal of Gastroenterology 2008年第23期14卷 3754-3758页

作者： Rong-Hua Xu liang-yan zheng Dong-Lei He Jian Tong Li-Ping zheng Wu-Ping zheng Jin Meng Li-Ping Xia Cong-Jun Wang Ji-Lin Yi The Affiliated Hospital of Hainan Medical College Haikou 570102 Hainan Province China Department of General Surgery First People's Hospital Affiliated to Shanghai Jiaotong University Shanghai 200080 China Department of General surgery Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM:To evaluate the inhibitory effects of human fragile histidine triad(FHIT) gene on cell proliferation and apoptosis in human hepatocellular carcinoma line Hep3B in vitro. METHODS:A recombinant pcDNA3.1(+) /FHIT including the functional region of FHIT gene was constructed and transferred into human hepatocellular carcinoma cells in vitro. mRNA and protein expression of the FHIT gene in the transfected cells was detected by RT-PCR and Western blot,respectively. The effect of FHIT on proliferation was detected by MTT assay. Changes in cell cycle and apoptosis were assayed by flow cytometry. Five mice received subcutaneous transplantation of Hep3B-FHIT;5 mice received subcutaneous transplantation of normal Hep3B and Hep3B-C as controls. The body weight of nude mice and tumor growth were measured. RESULTS:RT-PCR and Western blot analysis showed that the expression level of FHIT-mRNA and FHIT protein was higher in Hep3B cells after infection withpcDNA3.1(+) /FHIT. The growth of Hep3B cells treated with pcDNA3.1(+) /FHIT was significantly inhibited. The pcDNA3.1(+) /FHIT-transfected Hep3B cells showed a significantly higher cell rate at G0-G1 phase and increased apoptosis in comparison with controls(P < 0.05) . The growth of transplanted tumor was inhibited markedly by FHIT. Tumors arising from the Hep3B-FHIT cells occurred much later than those arising from the Hep3B and Hep3B-C cells. The growth of Hep3B-FHIT cells was slow and the tumor volume was low. CONCLUSION:Transduction of FHIT gene inhibits the growth of human hepatocellular carcinoma cells and induces cell apoptosis in vivo and in vitro.

关键词： Hepatocellular carcinoma Gene therapy Fragile histidine triad gene

Joint Probabilistic Scheduling and Resource Allocation for Wireless Networked Control Systems

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IEEE/CAA Journal of Automatica Sinica 2025年第1期 258-260页

作者： Meng zheng Lei Zhang Wei liang IEEE the State Key Laboratory of Robotics and the Key Laboratory of Networked Control Systems Shenyang Institute of Automation Chinese Academy of Sciences the University of Chinese Academy of Sciences

Dear Editor,This letter presents a joint probabilistic scheduling and resource allocation method(PSRA) for 5G-based wireless networked control systems(WNCSs). As a control-aware optimization method, PSRA minimizes the linear quadratic Gaussian(LQG) control cost of WNCSs by optimizing the activation probability of subsystems, the number of uplink repetitions, and the durations of uplink and downlink phases. Simulation results show that PSRA achieves smaller LQG control costs than existing works.

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Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis

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Neural Regeneration Research 2025年第4期20卷 1103-1123页

作者： Xiahong Tang Nan zheng Qingming Lin yan You zheng Gong yangping Zhuang Jiali Wu Yu Wang Hanlin Huang Jun Ke Feng Chen Shengli Clinical Medical College of Fujian Medical University Fujian Medical UniversityFuzhouFujian ProvinceChina Department of Emergency Fujian Provincial HospitalFuzhouFujian ProvinceChina Fujian Provincial Key Laboratory of Emergency Medicine FuzhouFujian ProvinceChina The Second Department of Intensive Care Unit Fujian Provincial Hospital South BranchFuzhouFujian ProvinceChina

Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological *** preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are *** this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB ***,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by *** results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these *** conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关键词： bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria neuroinflammation oxidative stress pyroptosis reactive oxygen species

A Verifiable Trust-Based CP-ABE Access Control Scheme for Cloud-Assisted Renewable Energy Systems

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Computers, Materials & Continua 2025年第1期82卷 1211-1232页

作者： Jiyu Zhang Kehe Wu Ruomeng yan zheng Tian Yizhen Sun Yuxi Wu Yaogong Guo Department of Control and Computer Engineering North China Electric Power UniversityBeijing102206China State Grid Information&Communication Company of Hunan Electric Power Corporation Changsha410118China State Grid Hunan Electric Power Corporation Chenzhou Power Supply Branch Chenzhou423000China

Renewable Energy Systems(RES)provide a sustainable solution to climate warming and environmental pollution by enhancing stability and reliability through status acquisition and analysis on cloud platforms and intelligent processing on edge servers(ES).However,securely distributing encrypted data stored in the cloud to terminals that meet decryption requirements has become a prominent research ***,managing attributes,including addition,deletion,and modification,is a crucial issue in the access control scheme for *** address these security concerns,a trust-based ciphertext-policy attribute-based encryption(CP-ABE)device access control scheme is proposed for RES(TB-CP-ABE).This scheme effectivelymanages the distribution and control of encrypted data on the cloud through robust attribute key *** introducing trust management mechanisms and outsourced decryption technology,the ES system can effectively assess and manage the trust worthiness of terminal devices,ensuring that only trusted devices can participate in data exchange and access sensitive ***,the ES system dynamically evaluates trust scores to set decryption trust thresholds,thereby regulating device data access permissions and enhancing the system’s *** validate the security of the proposed TB-CP-ABE against chosen plaintext attacks,a comprehensive formal security analysis is conducted using the widely accepted random oraclemodel under the decisional q-Bilinear Diffie-Hellman Exponent(q-BDHE)***,comparative analysis with other schemes demonstrates that the TB-CP-ABE scheme cuts energy/communication costs by 43%,and scaleswell with rising terminals,maintaining average latency below 50ms,ensuring real-time service *** proposed scheme not only provides newinsights for the secure management of RES but also lays a foundation for future secure energy solutions.

关键词： Access control renewable energy systems(RES) ciphertext-policy attribute-based encryption(CP-ABE) security

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一种新的移动机器人环境模型

机器人 1999年第5期21卷 379-385页

作者：李强林良明颜国正上海交通大学电子信息学院 200030

环境建模是移动机器人路径规划的基础．针对部分已知或完全未知的环境，本文提出一种基于位置码的四叉树环境模型，通过建立四叉树节点位置码与其笛卡尔坐标的一一对应关系，将对四叉树的操作转换到位置码空间，从而简化了对四叉树的操... 详细信息

环境建模是移动机器人路径规划的基础．针对部分已知或完全未知的环境，本文提出一种基于位置码的四叉树环境模型，通过建立四叉树节点位置码与其笛卡尔坐标的一一对应关系，将对四叉树的操作转换到位置码空间，从而简化了对四叉树的操作．文中研究了基于位置码的四叉树的重要操作，并给出了详细的算法；基于该模型，作者利用Ａ算法进行路径规划的仿真研究，仿真结果证明了该模型的有效性．

关键词：路径规划位置码移动机器人智能机器人

Synergistic promotion strategy of “dual-site” and “dual-path” to enhance the C–C coupling between CO2 and HCHO driven by photoelectrocatalysis

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Journal of Energy Chemistry 2025年第1期100卷 39-49页

作者： Hongqiang yang Jianfeng zheng Guoyu Wang Yifei Gao Yatao yang Jia Li Xiaojin Han yan Cui Zhanggen Huang National Key Laboratory of High Efficiency and Low Carbon Utilization of Coal Institute of Coal Chemistry Chinese Academy of Science University of Chinese Academy of Science Shenyang University of Chemical Technology

Photoelectrocatalytic coupling CO2and volatile organic compounds （VOCs） is a promising green strategy for the synergistic conversion of the two carbon-containing resources to *** catalytic efficiency is always at the mercy of chemical inertness of CO2and the competitive hydrogen evolution of ***,a modified g-C3N4/ZnAl-LDH Z-scheme heterojunction catalyst with dual reaction site was rationally designed and precisely *** Faraday efficiency of ethanol reached 68.67%with a corresponding formation rate of 227.3μmol *** revealed by in-situ characterizations and density functional theory calculations,CO2and HCHO were absorbed at Zn site and N site,***,*CO generated from CO2and HCHO was converted to*CH3O and*CHO on the dual-active-site *** detailed reaction mechanism experiments indicated that C–C coupling only occurred between*CO and*CH3O in electrocatalysis *** from the“*CO+*CH3O”path,another“*CO+*CHO”coupling path was also detected in photoelectrocatalytic *** selectivity of ethanol was significantly enhanced due to the synthesis of dual-site catalyst and the dual-path coupling path between CO2and HCHO simultaneously driven by light and electricity.

关键词： Ethanol

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动态障碍物环境下移动机器人路径规划

上海交通大学学报 2002年第10期36卷 1430-1434页

作者：王于林良明颜国正上海交通大学电子信息学院上海200030

以机器人基本避障策略与启发式动态规划法相结合的方法 ,研究了在障碍物轨迹已知的动态环境中机器人的路径规划问题 .根据机器人与单个障碍物可能的碰撞情况 ,分别提出几种避障策略 ,策略内容为子目标和相应的速度 .根据这些策略 ,在机... 详细信息

以机器人基本避障策略与启发式动态规划法相结合的方法 ,研究了在障碍物轨迹已知的动态环境中机器人的路径规划问题 .根据机器人与单个障碍物可能的碰撞情况 ,分别提出几种避障策略 ,策略内容为子目标和相应的速度 .根据这些策略 ,在机器人当前视窗内 ,将形成一个策略树 .采用启发式动态规划算法 ,依据时间和路径费用最小原则 ,找出一个最优策略集 ,即最优子目标集 .

关键词：动态障碍物移动机器人路径规划启发式动态规划避障策略时间费用最小原则路径费用最小原则

Investigation on crystal growth of A3BC3D2014 type piezoelectric crystals with Bridgman method

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Investigation on crystal growth of A3BC3D2014 type piezoelec...

2011年全国压电和声波理论及器件应用研讨会

作者： An-hua WU,Zhan-liang WANG,yan-qing zheng,Jun XU Shanghai Institute of Ceramics,Chinese Academy of Science,Shanghai 200050

From 1990’s,lanthanum gallium silicate(langasite,LaGaSiO,has attracted much attention as a potential substrate material for piezoelectric applications because of its high frequency stability and fairly electromechani... 详细信息

关键词： surface acoustic wave material Bridgman method A3BC3D2O14-type crystals

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移动机器人的自学习导航策略

上海交通大学学报 2001年第5期35卷 779-782,786页

作者：李强林良明颜国正上海交通大学电子信息学院上海200030

针对标准 Q学习收敛较慢的缺点 ,采用多步 Q学习算法 ;为解决连续过程的学习问题 ,采用 CMAC神经网络对连续状态空间进行泛化 .讨论了基于 CMAC的多步 Q学习算法应用于导航系统的实现方法 ,并与其他方法进行仿真比较 ,结果表明了该算法... 详细信息

针对标准 Q学习收敛较慢的缺点 ,采用多步 Q学习算法 ;为解决连续过程的学习问题 ,采用 CMAC神经网络对连续状态空间进行泛化 .讨论了基于 CMAC的多步 Q学习算法应用于导航系统的实现方法 ,并与其他方法进行仿真比较 ,结果表明了该算法的有效性 .

关键词：导航 Q学习 CMAC神经网络移动机器人