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Light-responsive Self-Immolative L-glutamic Acid-based Polyester Nanoparticles for Controlled Drug Release via Passerini Three-Component Polymerization

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Chinese Journal of Polymer Science 2024年第5期42卷 570-578,I0005页

作者： Xiao-Fei Sun Xu Zhang Shu-Ping Song Ya-Qun Yao Yan Zhang Cheng-liang Wang Jing-jiang Sun Qing-Fu Wang Key Laboratory of Rubber-plastics Ministry of EducationSchool of Polymer Science and EngineeringQingdao University of Science and TechnologyQingdao266042China Qingdao Research Academy of Environmental Science Qingdao266012China

L-glutamic acid(LA)is a bio-based,non-toxic,environmentally friendly material derived from *** present study reports the application of Passerini three-component polymerization(P-3CP)for the straightforward preparation of LA-based light-responsive polyesters(PLTDs)under mild *** with molar masses up to 8500 g/mol and high yields exceeding 90%are *** chemical structures and light-responsive self-immolative behavior of PLTDs are comprehensively characterized by employing ultraviolet-visible(UV-Vis)spectroscopy,size exclusion chromatography(SEC),nuclear magnetic resonance(NMR)spectroscopy,and liquid chromatography mass spectrometry(LC-MS).Meanwhile,monodisperse PLTD-based doxorubicin-loaded nanoparticles(PLTD-DOX-NP)(size=193 nm,PDI=0.018)are formulated by nanoprecipitation *** light-induced depolymerization,the PLTD-DOX-NP undergoes rapid decomposition,resulting in a burst release of 80%cargo within 13 ***,according to biological toxicity tests,the PLTD-NP possesses adequate biosafety,both before and after ***,the incorporation of P-3CP with biorenewable LA-based monomer adheres to the principles of green chemistry,significantly simplifying the synthetic pathway of light-responsive polymers.

关键词： L-glutamic acid Passerini three-component polymerization Light-responsive Polyester Nanoparticles

Asialoglycoprotein receptor 1 promotes SARS-CoV-2 infection of human normal hepatocytes

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Signal Transduction and Targeted Therapy 2024年第3期9卷 1199-1208页

作者： Xinyi Yang Xu Zheng Yuqi Zhu Xiaying Zhao Jun Liu jiangna Xun Songhua Yuan Jun Chen Hanyu Pan Jinlong Yang Jing Wang Zhimin liang Xiaoting Shen Yue liang Qinru Lin Huitong liang Min Li Fei Peng Daru Lu Jianqing Xu Hongzhou Lu Shibo jiang Ping Zhao Huanzhang Zhu State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology Ministry of EducationInstitute of GeneticsSchool of Life SciencesYiwu Research InstituteFudan UniversityShanghaiChina Department of Microbiology Faculty of Naval MedicineNaval Medical UniversityShanghai 200433China Scientific Research Center Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Department of Infectious Diseases and Immunology Shanghai Public Health Clinical CenterFudan UniversityShanghaiChina Changzheng Hospital Naval Medical UniversityShanghaiChina Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) Shanghai Institute of Infectious Disease and BiosecuritySchool of Basic Medical SciencesFudan UniversityShanghaiChina Department of Infectious Diseases and Nursing Research Institution National Clinical Research Center for Infectious DiseasesThe Third People’s Hospital of ShenzhenShenzhenGuangdongChina

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)causes multi-organ damage,which includes hepatic dysfunction,as observed in over 50%of COVID-19 *** I converting enzyme(peptidyl-dipeptidase A)2(ACE2)is the primary receptor for SARS-CoV-2 entry into host cells,and studies have shown the presence of intracellular virus particles in human hepatocytes that express ACE2,but at extremely low ***,we asked if hepatocytes might express receptors other than ACE2 capable of promoting the entry of SARS-CoV-2 into *** address this question,we performed a genome-wide CRISPR-Cas9 activation library screening and found that Asialoglycoprotein receptor 1(ASGR1)promoted SARS-CoV-2 pseudovirus infection of HeLa *** Huh-7 cells,simultaneous knockout of ACE2 and ASGR1 prevented SARS-CoV-2 pseudovirus *** the immortalized THLE-2 hepatocyte cell line and primary hepatic parenchymal cells,both of which barely expressed ACE2,SARSCoV-2 pseudovirus could successfully establish an ***,after treatment with ASGR1 antibody or siRNA targeting ASGR1,the infection rate significantly dropped,suggesting that SARS-CoV-2 pseudovirus infects hepatic parenchymal cells mainly through an ASGR1-dependent *** confirmed that ASGR1 could interact with Spike protein,which depends on receptor binding domain(RBD)and N-terminal domain(NTD).Finally,we also used Immunohistochemistry and electron microscopy to verify that SARS-CoV-2 could infect primary hepatic parenchymal *** inhibiting ASGR1 in primary hepatic parenchymal cells by siRNA,the infection efficiency of the live virus decreased ***,these findings indicate that ASGR1 is a candidate receptor for SARS-CoV-2 that promotes infection of hepatic parenchymal cells.

关键词： ACE2 infection glycoprotein

Glycogen metabolism-mediated intercellular communication in the tumor microenvironment influences liver cancer prognosis

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Oncology Research 2024年第3期32卷 563-576页

作者： YANG ZHANG NANNAN QIN XIJUN WANG RUI liang QUAN LIU RUOYI GENG TIANXIAO jiang YUNFEI LIU JINWEI LI Graduate School Kunming Medical UniversityKunming650000China Depatment of Vascular Surgery Fuwai Yunnan Cardiovascular HospitalAfliated Cardiovascular Hospital of Kunming Medical UniversityKunming650000China Department of Neurosurgery West China HospitalSichuan UniversityChengdu610000China Department of Neurosurgery The Fourth Afiliated Hospital of Guangxi Medical UniversityLiuzhou545000China Depatment of Gynecology Oncology The Fourth Afliated Hospital of Guangxi Medical UniversityLiuzhou545000China School of Basic Medical Sciences Xianning Medical CollegeHubei University of Science and TechnologyXianning437100China College of Bioengineering Chongqing UniversityChongqing400030China Department of General Visceraland Transplant SurgeryLudwig Maximilians University MunichMunich81377Germany

Glycogen metabolism plays a key role in the development of hepatoellular carcinoma(HCC),but the function of glycogen metabolism genes in the tumor microenvironment(TME)is still to be *** cell RNA-seq data were obtained from ten HCC tumor samples totaling 64,545 cells and 65 glycogen metabolism genes were analyzed bya nonnegative matrix factorization(NMF).The prognosis and immune response of new glycogen TME cell dusters were predicted by using HCC and immunotherapy cohorts from public *** single cell analysis was divided into fibroblasts,NT T cells,macrophages,endothelial clls,and B cells,which were separately divided into new cell clusters by glycogen metabolism gene *** temporal trajectory analysis demonstrated the temporal differentiation trajectory of different glycogen subtype cell *** communication analysis revealed extensive interactions between endothelial cells with glycogen metabolizing TME *** subtypes and diferent glycogen subtype cell *** analysis of transcription factors upstream of TME cell clusters with different glycogen *** addition,TME cell dusters of glycogen metabolism were found to be enriched in expression in CAF subtypes,CD8 depleted,M1,and M2 *** seq analysis showed the prognostic signifcance of glycogen *** TME cell dusters in HCC,while a significant immune response was found in the immunotherapy cohort in patients treated with immune checkpoint blockade(ICB),especially for CAFs,T cells,and macrophages In summary,our study reveals for the first time that glycogen metabolism mediates intercellular communication in the hepatocellular carcinoma microenvironment while elucidating the anti-tumor mechanisms and immune prognostic responses of different subtypes of cell dusters.

关键词： Glycogen metabolism Metabolic map Single cell Tumor microenvironment Liver cancer Prognosis Immunotherapy

Down-regulation of microRNA-23a promotes pancreatic ductal adenocarcinoma initiation and progression by up-regulation of FOXM1 expression

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Genes & Diseases 2024年第5期11卷 456-469页

作者： Lixin liang Tian Cai Xiaojia Li Jianhong An Sen Yu Yang Zhang Fengjie Guo Fang Wei Jie He Keping Xie Tingting jiang Center for Pancreatic Cancer Research South China University of Technology School of MedicineGuangzhouGuangdong 510006China Department of Laboratory Medicine The Sixth Affiliated Hospital and Nanhai People’s HospitalSouth China University of Technology School of MedicineFoshanGuangdong 528200China The Second Affiliated Hospital and Guangzhou First People’s Hospital South China University of Technology School of MedicineGuangzhouGuangdong 510006China

Transcriptional factor Forkhead box M1(FOXM1)plays an important role in pancreatic ductal adenocarcinoma(PDAC)development and *** molecular mechanisms underlying its dysregulation remain *** identified and functionally validated the microRNAs(miRNAs)that critically regulate FOXM1 expression in *** expression levels of miRNA-23a(miR-23a-3p and-5p)were altered in PDAC cell lines and their effects on FOXM1 signaling and cell proliferation and migration and tumorigenesis were examined in vitro and in vivo using mouse PDAC *** with non-tumor pancreatic tissues,PDAC tissues and cell lines exhibited significantly reduced levels of miR-23a *** miR-23a expression and concomitant increase in FOXM1 expression were also observed in acinar-toductal metaplasia and pancreatic intraepithelial neoplasia,the major premalignant lesions of *** expression of miR-23a reduced the expression of FOXM1 and suppressed cell proliferation and migration in PDAC cells,whereas the inhibitors of miR-23a did the *** or reduced levels of miR-23a increased the levels of FOXM1 expression,while increased expression of FOXM1 down-regulated miR-23a expression,suggesting that miR-23a and FOXM1 were mutual negative regulators of their expression in PDAC ***,the miR-23a/FOXM1 signaling axis is important in PDAC initiation and progression and could serve as an interventional or therapeutic target for patients with early or late stages of PDAC.

关键词： FOXM1 Gene expression Gene regulation MicroRNA Pancreatic cancer Targeted therapy

Optimizing high-coordination shell of Co-based single-atom catalysts for efficient ORR and zinc-air batteries

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Journal of Energy Chemistry 2024年第8期95卷 306-314,I0007页

作者： Yugang Qi Qing liang Kexin Song Xinyan Zhou Meiqi Liu Wenwen Li Fuxi Liu Zhou jiang Xu Zou Zhongjun Chen Wei Zhang Weitao Zheng Key Laboratory of Automobile Materials MOE School of Materials Science&Engineering Electron Microscopy Centerand International Center of Future ScienceJilin Provincial International Cooperation Key Laboratory of High-Efficiency Clean Energy MaterialsJilin UniversityChangchun 130012JilinChina Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China

Atom-level modulation of the coordination environment for single-atom catalysts(SACs)is considered as an effective strategy for elevating the catalytic *** the MNxsite,breaking the symmetrical geometry and charge distribution by introducing relatively weak electronegative atoms into the first/second shell is an efficient way,but it remains challenging for elucidating the underlying mechanism of ***,a practical strategy was reported to rationally design single cobalt atoms coordinated with both phosphorus and nitrogen atoms in a hierarchically porous carbon derived from metal-organic frameworks.X-ray absorption spectrum reveals that atomically dispersed Co sites are coordinated with four N atoms in the first shell and varying numbers of P atoms in the second shell(denoted as Co-N/P-C).The prepared catalyst exhibits excellent oxygen reduction reaction(ORR)activity as well as zinc-air battery *** introduction of P atoms in the Co-SACs weakens the interaction between Co and N,significantly promoting the adsorption process of ^(*)OOH,resulting in the acceleration of reaction kinetics and reduction of thermodynamic barrier,responsible for the increased intrinsic *** discovery provides insights into an ultimate design of single-atom catalysts with adjustable electrocatalytic activities for efficient electrochemical energy conversion.

关键词： Electrocatalytic Oxygen reduction reaction Single atom catalyst Shell coordination optimization

Harnessing cytokine-induced killer cells to accelerate diabetic wound healing:an approach to regulating post-traumatic inflammation

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Regenerative Biomaterials 2024年第2期3卷 88-99页

作者： Yixi Yang Cheng Zhang Yuan jiang Yijun He iawei Cai Lin liang Zhaohuan Chen Sicheng Pan Chu Hua Keke Wu Le Wang Zhiyong Zhang Translational Research Centre of Regenerative Medicine and 3D Printing of Guangzhou Medical University Guangdong Province Engineering Research Center for Biomedical EngineeringState Key Laboratory of Respiratory DiseaseDepartment of Orthopaedic SurgeryMedical Technology and Related Equipment Research for Spinal Injury TreatmentCity Key LaboratoryThe Third Affliated Hospital of Guangzhou Medical UniversitySchool of Biomedical EngineeringGuangzhou MedicalUniversityGuangzhouGuangdong510150P.R.China Department of Osteoarthropathy and Sports Medicine Panyu Central HospitalGuangzhou 511400P.R.China

Impaired immunohomeostasis in diabetic wounds prolongs infiammation and cytokine dysfunction,thus,delaying or pre-venting wound-surface *** clinical studies have been conducted on cytokine-induced killer(CIK)cells recently,as they can be easily proliferated using a straightforward,inex-pensive ***,the function of CIK cells in regulat-ing inflammatory environments has been drawing attention for clinical *** the current investigation,we discovered the regenerative capacity of these cells in the chal-lenging environment of wounds that heal poorly due to *** demonstrated that the intravenous injection of CIK cells can re-establish a proregenerative inflammatory *** *** accelerateskin healing in diabetic *** results indicated that CIK cell treatment affects macrophage polarization and restores the function regenerative cells under hyperglycemic *** novel cellular therapy offers a promising intervention for clinical applic tions through specific inflammatory regulation functions.

关键词： macrophage diabetic wound post-traumatic inflammation cytokine-induced killer cells vascularization

Microalgae-based drug delivery system for tumor microenvironment photo-modulating and synergistic chemo-photodynamic therapy of osteosarcoma

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Engineered Regeneration 2024年第2期5卷 199-209页

作者： Feng liang Xueying An Ruoxi Wang Wenshu Wu Lin Yang Yixin Zheng Qing jiang Xingquan Xu Danni Zhong Min Zhou Eye Center The Second Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China Department of Orthopedics The First Affiliated HospitalZhejiang University School of MedicineHangzhou310000PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalThe Affiliated Hospital of Nanjing University Medical SchoolNanjing210008PR China Zhejiang University-University of Edinburgh Institute(ZJU-UoE Institute) Zhejiang University School of MedicineZhejiang UniversityHaining314400PR China State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryNanjing Drum Tower HospitalClinical College of Nanjing Medical UniversityNanjing210008PR China Institute of Translational Medicine Zhejiang UniversityHangzhou310029PR China Zhejiang University-Ordos City Etuoke Banner Joint Research Center Zhejiang UniversityHaining314400PR China

Osteosarcoma(OS)is one of the most common malignant tumors in children and young *** chemotherapy and other therapies are limited by low therapeutic efficiency,severe side effects and single therapeutic function,it is of high value to develop innovative therapies for precise and efficient treatment of ***,natural photo-synthetic microalgae(***,CV)were utilized as carriers for the chemotherapeutic agent doxorubicin(DOX)to create a multifunctional therapeutic platform(CV@DOX)for the photo-modulation of the tumor microenvi-ronment(TME)and synergistic chemo-photodynamic therapy of ***@DOX exhibited rapid drug release behavior in the acidic TME,improving the efficiency of chemotherapy against tumors and reducing side effects on other normal *** 650 nm laser irradiation,CV@DOX demonstrated the ability to effectively generate oxygen to alleviate tumor hypoxia and utilize the photosensitizing properties of chlorophyll in CV to produce an increased amount of reactive oxygen species(ROS),thereby enhancing photodynamic therapy(PDT).CV@DOX-mediated synergistic chemo-photodynamic therapy demonstrated efficacy in halting tumor progression in an orthotopic osteosarcoma mouse model by promoting tumor cell apoptosis,inhibiting tumor proliferation and ***,chlorophyll-assisted fluorescence imaging enabled monitoring of the distribution of CV@DOX in osteosarcoma after ***,CV@DOX did not cause significant hematological and tissue toxicity,and prevented DOX-induced cardiotoxicity,showing good in vivo ***,this work presents a novel TME-responsive and TME-modulating platform for imaging-guided multimodal osteosar-coma treatment.

关键词： Osteosarcoma Tumor hypoxia Photodynamic Chemotherapy Synergistic therapy

Effect of Artificial Cooling Extrusion on Microstructure and Mechanical Properties of Mg–Zn–Y Alloys

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Acta Metallurgica Sinica(English Letters) 2024年第7期37卷 1115-1127页

作者： Qi-Yu Liao Da-Zhi Zhao Qi-Chi Le Wen-Xin Hu Yan-Chao jiang Wei-Yang Zhou liang Ren Dan-Dan Li Zhao-Yang Yin School of Materials Science and Engineering Northeastern UniversityShenyang110819China Key Laboratory of Electromagnetic Processing of Materials Ministry of EducationNortheastern UniversityShenyang110819China Key Laboratory of Lightweight Structural Materials Liaoning ProvinceNortheastern UniversityShenyang110819China Baotou Research Institute of Rare Earths Baotou014000China

Microstructure and mechanical properties of Mg–Zn–Y alloys with different Zn/Y atomic ratios with or without artificial cooling (AC) extrusion were systematically investigated in this work. The results show that bimodal microstructure consisting of submicron dynamic recrystallized (DRXed) grains with high fraction of low-angle grain boundaries (LAGBs) and elongated unDRXed grains was formed in Mg_(98.7)Zn_(1)Y_(0.3) alloy with AC extrusion. The AC process effectively limits the growth of precipitated phases, and large amount of nanoscale precipitates were dynamically precipitated during the extrusion process. AC extrusion could effectually refine the lamellar 14H LPSO phases and inhibit the transition from stacking faults to LSPO phases in Mg_(98)Zn_(1)Y_(1) alloy and the narrow LPSO phase in Mg_(98)Zn_(1)Y_(1)-AC alloy which could promote the nucleation of DRXed grains. The AC extrusion significantly improves the strength of Mg–Zn–Y alloys. Owing to AC extrusion, the strength improvement of Mg_(98.7)Zn_(1)Y_(0.3) alloy is mainly attributed to fine grain strengthening, dislocation strengthening, and nano-phases precipitation strengthening. After AC process, more fine grains and nano-phases jointly strengthen the Mg_(98)Zn_(1)Y_(1) alloy. The Mg_(98)Zn_(1)Y_(1) alloy obtains optimal mechanical properties after extrusion at 623 K, with ultimate tensile strength (UTS) of 406 MPa, yield strength (YS) of 388 MPa, and elongation (EL) of 5.6%.

关键词： Mg-Zn-Y Artificial cooling Extrusion Microstructure Mechanical properties

Microstructures and mechanical properties of laser-directed energy deposited CrCoNi medium-entropy alloy

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Rare Metals 2024年第7期43卷 3286-3300页

作者： Wen-Jie Zhao Chang-Yu Liu Peng-Cheng Che Zhi-liang Ning Hong-Bo Fan Jian-Fei Sun Yong-jiang Huang Alfonso H.W.Ngan National Key Laboratory for Precision Hot Processing of Metals Harbin Institute of TechnologyHarbin 150001China Space Environment Simulation Research Infrastructure Harbin Institute of TechnologyHarbin 150001China Department of Mechanical Engineering The University of Hong KongHong Kong 999077China

The distinctive intrinsic heat treatment(IHT)originating from cyclic reheating in the laser-directed energy deposition(LDED) has attracted growing attention in recent *** this investigation,simulations and experimental characterizations were performed to examine the impact of IHT on the micros true ture and mechanical properties of LDED-fabricated CrCoNi medium-entropy alloy(MEA).The results show that the intensity of the IHT is proportional to the utilized laser energy density(LED).As the LED increased,significant dynamic recrystallization and grain refinement occurred within the alloy due to the enhanced intensity of ***,the high LED leads to severe hot cracking within the as-built MEA,resulting in inferior *** decreasing LED,the hot cracking was effectively ***,low LED weakened the intensity of IHT and consequently inhibited kinetic conditions of dynamic recrystallization,resulting in a heterogeneous grain structure characterized by multi-scale-sized *** structure provides significant hetero-deformation-induced hardening during plastic deformation,enabling the alloy to have a sustainable work-hardening *** expect that this work will have implications in taking full advantage of the unique IHT of the LDED process to fabricate ME As with excellent metallurgical quality and mechanical performance.

关键词： Intrinsic heat treatment Laser-directed energy deposition CrCoNi MEA Dynamic recrystallization Heterogeneous grain structure