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Blood exosomal micro ribonucleic acid profiling reveals the complexity of hepatocellular carcinoma and identifies potential biomarkers for differential diagnosis

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World Journal of Gastrointestinal Oncology 2020年第10期12卷 1195-1208页

作者： Lang-qing Sheng jia-Rong Li Hao qin Ling Liu Da-Dong zhang Qi zhang Meng-Li Huang Xiao-Li Li Xiao-Ya xu Yang-Nian wei Zi-Shuo Chen Hui Luo ji-Yang zhang Cheng-Hui Zhou Hao Chen Ze-Guo Chen Fu-Gen Li Nian-feng Li Department of Hepatobiliary and Pancreatic Surgery Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina Department of Biliopancreatic Surgery Xiangya HospitalCentral South UniversityChangsha 410008Hunan ProvinceChina Research and Development Institute of Precision Medicine 3D Medicines Inc.Shanghai 201114Hunan ProvinceChina The Medical Department 3D Medicines Inc.Shanghai 201114China

BACKGROUND Hepatocellular carcinoma(HCC)is one of the leading causes of cancer-related deaths worldwide,but there is a shortage of effective biomarkers for its *** To explore blood exosomal micro ribonucleic acids(miRNAs)as potential biomarkers for HCC *** The principal component analysis suggested that daily alcohol consumption could alter the blood exosomal miRNA profiles of hepatitis B virus positive non-HCC patients through miR-3168 and *** miRNA profiles also revealed the tumor stages of HCC *** expression of miR-455-5p and miR-30c-5p,which significantly correlated with better overall survival in tumor tissues,could also be detected in blood *** pairs of miRNAs(miR-584-5p/miR-106-3p and miR-628-3p/miR-941)showed a 94.1%sensitivity and 68.4%specificity to differentiate HCC patients from non-HCC *** specificity of the combination was substantially influenced by alcohol consumption *** This study suggested that blood exosomal miRNAs can be used as new noninvasive diagnostic tools for ***,their accuracy could be affected by tumor stage and alcohol consumption habits.

关键词： Blood exosomal micro ribonucleic acids Biomarker Differential diagnosis Alcohol consumption habit Hepatocellular carcinoma Bioinformatics

食管静脉曲张套扎术后早期再出血的危险因素探讨

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中国内镜杂志 2010年第4期16卷 344-347页

作者：季峰徐勤伟张咩庆浙江大学医学院附属第一医院消化内科浙江杭州310003

目的探讨肝硬化食管静脉曲张内镜下套扎术(EVL)后早期再出血的危险因素。方法回顾性分析259例接受EVL术肝硬化患者的一般情况、病因、手术史、临床表现、胃镜检查、饮食及运动负荷、药物治疗及实验室检查等情况,寻找独立危险因素。结果 ... 详细信息

目的探讨肝硬化食管静脉曲张内镜下套扎术(EVL)后早期再出血的危险因素。方法回顾性分析259例接受EVL术肝硬化患者的一般情况、病因、手术史、临床表现、胃镜检查、饮食及运动负荷、药物治疗及实验室检查等情况,寻找独立危险因素。结果 EVL术后早期再出血率为8.49%,再出血死亡率为31.8%。摄入硬食、腹水量、凝血酶原时间、门静脉血栓及血红蛋白为EVL术后早期再出血的独立危险因素。结论 EVL术前纠正严重受损的肝功能、大量腹水、凝血功能异常及术后避免硬食可减少术后早期再出血的发生。

关键词：食管静脉曲张破裂出血套扎术早期再出血危险因素

Measurement of the neutron total cross section of carbon at the Back-n white neutron beam of CSNS

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Nuclear Science and Techniques 2019年第9期30卷 73-82页

作者： Xing-Yan Liu Yi-wei Yang Rong Liu jie Wen Zhong-wei Wen Zi-jie Han Zhi-Zhou Ren Qi An Huai-Yong Bai jie Bao Ping Cao Qi-Ping Chen Yong-Hao Chen Pin-jing Cheng Zeng-Qi Cui Rui-Rui Fan Chang-qing feng Min-Hao Gu feng-qin Guo Chang-Cai Han Guo-Zhu He Yong-Cheng He Yue-feng He Han-Xiong Huang wei-Ling Huang Xi-Ru Huang Xiao-Lu ji xu-Yang ji Hao-Yu jiang wei jiang Han-Tao jing Ling Kang Ming-Tao Kang Bo Li Lun Li Qiang Li Xiao Li Yang Li Yang Li Shu-Bin Liu Guang-Yuan Luan Ying-Lin Ma Chang-Jun Ning Bin-Bin Qi jie Ren Xi-Chao Ruan Zhao-Hui Song Hong Sun Xiao-Yang Sun Zhi-jia Sun Zhi-Xin Tan Hong-qing Tang jing-Yu Tang Peng-Cheng Wang Qi Wang Tao-feng Wang Yan-feng Wang Zhao-Hui Wang Zheng Wang qing-Biao Wu Xiao-Guang Wu xuan Wu Li-Kun Xie Han Yi Li Yu Tao Yu Yong-ji Yu Guo-Hui zhang jing zhang Lin-Hao zhang Li-Ying zhang qing-Min zhang Qi-wei zhang Xian-Peng zhang Yu-liang zhang Zhi-Yong zhang Ying-Tan Zhao liang Zhou Zu-Ying Zhou Dan-Yang Zhu Ke-Jun Zhu Peng Zhu Institute of Nuclear Physics and Chemistry China Academy of Engineering Physics State Key Laboratory of Particle Detection and Electronics Chinese Academy of Sciences(CAS) Department of Modern Physics University of Science and Technology of China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking University Key Laboratory of Nuclear Data China Institute of Atomic Energy Institute of High Energy Physics Chinese Academy of Sciences(CAS) Spallation Neutron Source Science Center University of South China Northwest Institute of Nuclear Technology Department of Engineering and Applied Physics University of Science and Technology of China Beihang University Xi’an jiaotong University

To verify the performance of the neutron total cross-sectional spectrometer, the neutron total cross section of carbon is initially measured in the energy range of 1 eV to 20 MeV using the time-of-flight method. The measurement is performed at the Back-n white neutron source with a 76-m time-of-flight path using the China Spallation Neutron Source. A multilayer fast fission chamber with 235U and 238U is employed as the neutron detector. The diameter and thickness of the natural graphite sample are 70 mm and 40 mm, respectively. Signal waveforms are collected using a data acquisition system. Off-line data processing was used to obtain the neutron time-of-flight spectra and transmissions. The uncertainty of the counting statistics is generally approximately 3% for each bin in the energy range of 1–20 MeV. It is determined that the results for the neutron total cross section of carbon obtained using ^235U cells are in good agreement with the results obtained using 238U cells within limits of statistical uncertainty. Moreover, the measured total cross sections show good agreement with the broadening evaluated data.

关键词： CSNS Back-n white neutron source NTOX Neutron total cross section Carbon Multilayer fast fission chamber

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反应精馏制备醋酸异辛酯新工艺的模拟研究

现代化工 2013年第12期33卷 126-129页

作者：徐红秦继伟李旭峰张浩熊晓娟叶青常州大学石油化工学院江苏常州213164 上海制皂(集团)如皋有限公司江苏如皋226521

对以异辛醇、醋酸为原料,以阳离子交换树脂为催化剂,使用环己烷作为夹带剂合成醋酸异辛酯的反应精馏过程进行了研究。通过模拟,考察了加入夹带剂环己烷后,该反应精馏体系中精馏段、反应段、提馏段塔板数以及反应段进料位置等因素对分离... 详细信息

对以异辛醇、醋酸为原料,以阳离子交换树脂为催化剂,使用环己烷作为夹带剂合成醋酸异辛酯的反应精馏过程进行了研究。通过模拟,考察了加入夹带剂环己烷后,该反应精馏体系中精馏段、反应段、提馏段塔板数以及反应段进料位置等因素对分离效果的影响。结果显示,反应精馏塔精馏段塔板数1块,反应段塔板数4块,提馏段塔板数7块,异辛醇从第2块塔板进料,醋酸从第5块塔板进料,在此条件下,塔釜出料中醋酸异辛酯的摩尔分数可达99.90%,而常规反应精馏的摩尔分数达到97.44%。将2种反应精馏进行比较,该过程改进以后,减少了塔板总数,节省了设备投资。

关键词：反应精馏醋酸异辛酯环己烷 Aspen Plus

The first data release(DR1) of the LAMOST regular survey

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Research in Astronomy and Astrophysics 2015年第8期15卷 1095-1124页

作者： A-Li Luo Yong-Heng Zhao Gang Zhao Li-Cai Deng Xiao-wei Liu Yi-Peng jing Gang Wang Hao-Tong zhang jian-Rong Shi Xiang-Qun Cui Yao-Quan Chu Guo-Ping Li Zhong-Rui Bai Yue Wu Yan Cai Shu-Yun Cao Zi-Huang Cao Jeffrey L.Carlin Hai-Yuan Chen jian-Jun Chen Kun-Xin Chen Li Chen xue-Lei Chen Xiao-Yan Chen Ying Chen Norbert Christlieb jia-Ru Chu Chen-Zhou Cui Yi-Qiao Dong Bing Du Dong-wei Fan Lei feng jian-Ning Fu Peng Gao xue-Fei Gong Bo-Zhong Gu Yan-Xin Guo Zhan-Wen Han Bo-liang He jin-liang Hou Yong-Hui Hou Wen Hou Hong-Zhuan Hu Ning-Sheng Hu Zhong-Wen Hu Zhi-Ying Huo Lei jia Fang-Hua jiang Xiang jiang Zhi-Bo jiang Ge jin Xiao Kong xu Kong Ya-Juan Lei Ai-Hua Li Chang-Hua Li Guang-wei Li Hai-Ning Li jian Li Qi Li Shuang Li Sha-Sha Li Xin-Nan Li Yan Li Yin-Bi Li Ye-Ping Li Yuan liang Chien-Cheng Lin Chao Liu Gen-Rong Liu Guan-Qun Liu Zhi-Gang Liu Wen-Zhi Lu Yu Luo Yin-Dun Mao Heidi Newberg ji-Jun Ni Zhao-Xiang Qi Yong-Jun Qi Shi-Yin Shen Huo-Ming Shi jing Song Yi-Han Song Ding-Qiang Su Hong-Jun Su Zheng-Hong Tang qing-Sheng Tao Yuan Tian Dan Wang Da-Qi Wang feng-Fei Wang Guo-Min Wang Hai Wang Hong-Chi Wang jian Wang jia-Ning Wang jian-Ling Wang jian-Ping Wang Jun-Xian Wang Lei Wang Meng-Xin Wang Shou-Guan Wang Shu-qing Wang Xia Wang Ya-Nan Wang You Wang Yue-Fei Wang You-Fen Wang Peng wei Ming-Zhi wei Hong Wu Ke-Fei Wu xue-Bing Wu Yu-Zhong Wu Xiao-Zheng Xing Ling-Zhe xu Xin-Qi xu Yan xu Tai-Sheng Yan De-Hua Yang Hai-feng Yang Hui-qin Yang Ming Yang Zheng-Qiu Yao Yong Yu Hui Yuan Hai-Bo Yuan Hai-Long Yuan wei-Min Yuan Chao Zhai En-Peng zhang Hua-wei zhang jian-Nan zhang Li-Pin zhang wei zhang Yong zhang Yan-Xia zhang Zheng-Chao zhang Ming Zhao Fang Zhou xu Zhou jie Zhu Yong-Tian Zhu Si-Cheng Zou Fang Zuo National Astronomical Observatories Chinese Academy of Sciences Department of Astronomy Peking University Shanghai Astronomical Observatory Chinese Academy of Sciences Nanjing Institute of Astronomical Optics & Technology National Astronomical ObservatoriesChinese Academy of Sciences University of Science and Technology of China Department of Physics Applied Physics and AstronomyRensselaer Polytechnic InstituteTroyNY 12180USA Department of Physics and Astronomy Earlham CollegeRichmondIN 47374USA Universitt Heidelberg Zentrum für AstronomieLandessternwarteKnigstuhl 1269117 HeidelbergGermany Department of Astronomy Beijing Normal University Yunnan Observatories Chinese Academy of Sciences Purple Mountain Observatory Chinese Academy of Sciences

The Large sky Area Multi-Object Fiber Spectroscopic Telescope（LAMOST） general survey is a spectroscopic survey that will eventually cover approximately half of the celestial sphere and collect 10 million spectra of stars, galaxies and QSOs. Objects in both the pilot survey and the first year regular survey are included in the LAMOST DR1. The pilot survey started in October 2011 and ended in June 2012, and the data have been released to the public as the LAMOST Pilot Data Release in August 2012. The regular survey started in September 2012, and completed its first year of operation in June 2013. The LAMOST DR1 includes a total of 1202 plates containing 2 955 336 spectra, of which 1 790 879 spectra have observed signalto-noise ratio（SNR） ≥ 10. All data with SNR ≥ 2 are formally released as LAMOST DR1 under the LAMOST data policy. This data release contains a total of 2 204 696 spectra, of which 1 944 329 are stellar spectra, 12 082 are galaxy spectra and 5017 are quasars. The DR1 not only includes spectra, but also three stellar catalogs with measured parameters： late A,FGK-type stars with high quality spectra（1 061 918 entries）, A-type stars（100 073 entries）, and M-type stars（121 522 entries）. This paper introduces the survey design, the observational and instrumental limitations, data reduction and analysis, and some caveats. A description of the FITS structure of spectral files and parameter catalogs is also provided.

关键词： techniques: spectroscopic survey—data release—catalog

Lung-protective Ventilation in Patients with Brain Injury： A Multicenter Cross-sectional Study and Questionnaire Survey in China

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Chinese Medical Journal 2016年第14期129卷 1643-1651页

作者： xu-Ying Luo Ying-Hong Hu Xiang-Yuan Cao Yan Kang Li-Ping Liu Shou-Hong Wang Rong-Guo Yu Xiang-You Yu Xia zhang Bao-Shan Li Zeng-Xiang Ma Yi-Bing Weng Heng zhang De-Chang Chen wei Chen Wen-jin Chen Xiu-Mei Chen Bin Du Mei-Li Duan jin Hu Yun-feng Hoang Gui-Jun jia Li-Hong Li Yu-Min liang Bing-Yu qin Xian-Dong Wang jian Xiong Li-Mei Yan Zheng-Ping Yang Chen-Ming Dong Dong-Xin Wang qing-Yuan Zhan Shuang-Lin FU Lin Zhao Qi-Bing Huang Ying-Guang Xie Xiao-Bo Huang Guo-Bin zhang Wang-Bin xu Yuan xu YaLing Liu He-Ling Zhao Rong-qing Sun Ming Sun qing-Hong Cheng Xin Qu Xiao-feng Yang Ming xu Zhong-Hua Shi Han Chen xuan He Yan-Lin Yang Guang-Qiang Chen Xiu-Mei Sun jian-Xin Zhou Department of Critical Care Medicine Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Critical Care Medicine Second Affiliated Hospital of Zhejiang University School of Medicine Hangzhou Zhejiang 310009 China Department of Critical Care Medicine General Hospital of Ningxia Medical University Yinchuan Ningxia 750004 China Department of Critical Care Medicine West China Hospital Sichuan University Chengdu Sichuan 610041 China Neurological Intensive Care Unit Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Critical Care Medicine Guangdong General Hospital Guangzhou Guangdong 510000 China Surgical Intensive Care Unit Fujian Province Hospital Fuzhou Fujian 350000 China Department of Critical Care Medicine First Affiliated Hospital of Xinjiang Medical University Urumqi Xinjiang 830054 China Department of Critical Care Medicine Bethune International Peace Hospital Shijiazhuang Hebei 050000 China Department of Critical Care Medicine Yuncheng Central Hospital Yuncheng Shanxi 044000 China Department of Emergency and Critical Care Medicine Cangzhou People's Hospital Cangzhou Hebei 061000 China Department of Critical Care Medicine Beijing Luhe Hospital Capital Medical University Beijing 101100 China Department of Critical Care Medicine First Hospital of China Medical University Shenyang Liaoning 110000 China Department of Critical Care Medicine Shanghai Changzheng Hospital Shanghai 200000 China Department of Critical Care Medicine Beijing Shijitan Hospital Capital Medical University Beijing 100038 China Department of Critical Care Medicine Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Critical Care Medicine Miyun County Hospital Beijing 100038 China Medical Intensive Care Unit Peking Union Medical College Hospital Beijing 101500 China Department of Critical Care Medicine Beijing Friendship Hospital Capital Medical University Beijing 100050 Chin

Background： Over the years, the mechanical ventilation （MV） strategy has changed worldwide. The aim of the present study was to describe the ventilation practices, particularly lung-protective ventilation （LPV）, among brain-injured patients in China. Methods： This study was a multicenter, 1-day, cross-sectional study in 47 Intensive Care Units （ICUs） across China. Mechanically ventilated patients （18 years and older） with brain injury in a participating ICU during the time of the study, including traumatic brain injury, stroke, postoperation with intracranial tumor, hypoxic-ischemic encephalopathy, intracranial infection, and idiopathic epilepsy, were enrolled. Demographic data, primary diagnoses, indications for MV, MV modes and settings, and prognoses on the 60th day were collected. Multivariable logistic analysis was used to assess factors that might affect the use of LPV. Results： A total of 104 patients were enrolled in the present study, 87 （83.7%） of whom were identified with severe brain injury based on a Glasgow Coma Scale 〈8 points. Synchronized intermittent mandatory ventilation （SIMV） was the most frequent ventilator mode, accounting for 46.2% of the entire cohort. The median tidal volume was set to 8.0 ml/kg （interquartile range [IQR], 7.0-8.9 ml/kg） of the predicted body weight; 50 （48.1%） patients received LPV. The median positive end-expiratory pressure （PEEP） was set to 5 cmH20 （IQR, 5-5 cmH20）. No PEEP values were higher than 10 cmH20. Compared with partially mandatory ventilation, supportive and spontaneous ventilation practices were associated with LPV. There were no significant differences in mortality and MV duration between patients subjected to LPV and those were not. Conclusions： Among brain-injured patients in China, SIMV was the most frequent ventilation mode. Nearly one-half of the brain-injured patients received LPV. Patients under supportive and spontaneous ventilation were more likely to receive LPV.

关键词： Brain Injury Epidemiology Lung-protective Ventilation Mechanical Ventilation

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结节性硬化症家系临床特点与基因分析

中国现代神经疾病杂志 2012年第3期12卷 300-306页

作者：李亚勤操基清杨娟张保锋冯善伟王艳云张瑶陈菲孔杰郑民缨张旭张成中山大学干细胞与组织工程研究中心广州510080 中山大学附属第一医院神经科广州510080 中山大学附属第一医院康复科广州510080 广州市人口和计划生育科学研究所 510410 哈尔滨医科大学第一临床医学院神经科 150001 解放军空军航空医学院研究所分子病理中心北京100089

目的分析结节性硬化症家系患者的临床与影像学表现,以及基因突变特点,增加对其认识。方法收集结节性硬化症三家系临床资料并进行回顾分析。应用聚合酶链反应和DNA直接测序法对家系1先证者进行TSC2基因检测及分析,明确其基因突变位点。... 详细信息

目的分析结节性硬化症家系患者的临床与影像学表现,以及基因突变特点,增加对其认识。方法收集结节性硬化症三家系临床资料并进行回顾分析。应用聚合酶链反应和DNA直接测序法对家系1先证者进行TSC2基因检测及分析,明确其基因突变位点。结果三家系先证者均以癫发作为首发症状,伴精神发育迟滞、自闭倾向、皮肤白斑脱失。影像学检查显示颅内结节性钙化点。脑电图呈棘慢复合波、尖波。家系1中三代共5例患病,男性2例、女性3例,均有面部皮脂腺瘤和癫发作症状和体征,符合常染色体显性遗传特点。先证者TSC2基因分析显示c.14442A>C基因突变。结论对以癫发作为首发症状,同时伴有精神发育迟滞、自闭倾向,以及皮脂腺瘤或色素脱失斑等皮肤异常表现的婴幼儿,当影像学检查显示颅内结节性钙化点时,高度提示结节性硬化症。通过对TSC1和TSC2基因分析可明确其基因突变位点,有助于对患病家系成员进行遗传学咨询和产前诊断。

关键词：结节性硬化症基因显性体层摄影术 X线计算机磁共振成像系谱

Consensus statement on layout and delivery of automatic external defibrillator in China

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Journal of Hainan Medical University 2020年第15期26卷 18-23页

作者： Yu Cao Song Chen Yan-Fen Chai Xiao-Hui Chen Yu-Guo Chen Shao-Wen Cheng Wen-wei Cheng Bang-Han Ding Yang Deng jie Fu Bang-jiang Fang Mai-Ying Fan Li Gui Ping Gong wei Guo Shu-Bin Guo liang Huang ji-Yuan Han Zhong ji Xian-Fei ji Xin-Cai ji Hong-xu jin Xin-Jun jiang Wang-Xiang jiang jia-liang Liu Xiao-Ran Liu Zhi Liu Chuan-Zhu Lv feng Lu Tan-Shi Li Xin Li Yong Li Shang-Lun Li Shuang-Ming Li Xiao-Gang Li Bao-Jun Li Pei-Wu Li Chun-Sheng Li Zhi-Jun Li Chang-Luo Li Xiang-Min Li Yin-Ping Li Zong-Jun Lu Zu-xun Lu Zhong-Qiu Lu Zhi-Qian Luo Xiao-Xing Liao jian-Bo Lei Tao Ma Yu Ma Yue-feng Ma qing-Bian Ma Lin-Hao Ma Jun-Qi Ma Shi-Nan Nie jie-Miao Ou-Yang Peng Peng Qiao Pei Shu-Ming Pan Guo-Zhong qing Li-jie qin Wu-Ying Qiao Fei Shao wei Song Xing-Yue Song Zhen-Ju Song Rong-Ju Sun De-Ya Shang Du-Juan Sha Gui-Lan Tao Guo-Gang Tian Ying-Ping Tian jian-Guang Tian Chao-Yang Tong Zi-Ren Tang Meng-Xiong Tang Guo-Ping Wu Zhi Wan Tao Wang Xin Wang Peng Wang Zhong Wang Tong Wang Ri-Xing Wang Yi-Xin Wang Xiao-Dan Wang Ling Wu wei wei Hua Xiang feng xu Jun xu jie xu Miao-Rong Xie Shuo-Gui xu Tie xu feng Yu Wen Yi Li-Shan Yang Shi-jiao Yan jin-jian Yao Shou-Chun Yan xue-Zhong Yu Xian-Zhi Ye Xian-liang Yan Fu-Bing Zhou Rong-Bin Zhou Ming Zhou Qiang Zhou jin Zhou Ping Zhou Chang-Ju Zhu Hua-Dong Zhu ji-Hong Zhu Hong zhang Mao zhang Hua zhang Na zhang Guo-Qiang zhang Guo-Xiu zhang jin-Song zhang Wen-Wu zhang Wen-Zhong zhang jian-feng zhang Yi-Min Zhu Jun Zeng Yuan Zheng Zhi-jie Zheng Shen Zhao Yan Zhao Min Zhao Bin Zhao Xiao-Dong Zhao Xiao-jing Zhao Hong-Ke Zeng Sheng-Qiang Zou The Chinese Society of Emergency Medicine Research Unit of Island Emergency Medicine Chinese Academy of Medical SciencesHainan Medical University Key Laboratory of Emergency and Trauma of Ministry of Education Hainan Medical UniversityHaikouHainanChina Emergency and Trauma College Hainan Medical UniversityHaikouHainanChina Department of Emergency Hainan Clinical Research Center for Acute and Critical DiseasesThe Second Affiliated Hospital of Hainan Medical UniversityHaikouHainanChina Key Laboratory of Hainan Trauma and Disaster Rescue Key Laboratory of Trauma of Hainan Medical University

*** Sudden cardiac death(SCD)refers to sudden death due to a variety of cardiac *** is manifested as a sudden loss of consciousness,cardiac arrest,and respiratory arrest and generally shows early symptoms that are atypical and not easily detected[1].According to a recent study,nearly 544000 patients suffer from SCD every year in China[2].

关键词： cardiac death respiratory

盾果藻Carpopeltis affinis多糖的提取分离、理化性质及抗病毒活性研究

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中国海洋药物 2023年第2期42卷 10-16页

作者：孙继芹牛庆凤秦吉张烜艾旭泽于广利王伟李国云中国海洋大学海洋药物教育部重点实验室山东省糖科学与糖工程重点实验室医药学院山东青岛266003 青岛海洋科学与技术国家实验室海洋药物与生物制品功能实验室山东青岛266237 青岛市生态环境局市南分局山东青岛266003

目的采用不同方法提取盾果藻Carpopeltis affinis中多糖,对其理化性质和结构进行分析,并初步评价其抗病毒活性。方法依次通过冷水、热水(80℃)和2%NaOH溶液提取盾果藻多糖,通过硫酸苯酚、离子色谱法、PMP柱前衍生高效液相色谱法等对其... 详细信息

目的采用不同方法提取盾果藻Carpopeltis affinis中多糖,对其理化性质和结构进行分析,并初步评价其抗病毒活性。方法依次通过冷水、热水(80℃)和2%NaOH溶液提取盾果藻多糖,通过硫酸苯酚、离子色谱法、PMP柱前衍生高效液相色谱法等对其总糖、硫酸根含量、单糖组成等进行分析,利用核磁共振波谱技术初步探究其结构。通过细胞病变效应(CPE)和假病毒抑制实验初步探究其抗病毒活性。结果3种多糖冷水提取(CCAP)、热水提取(HCAP)、2%NaOH提取(ACAP)均主要含硫酸半乳糖,热水浸提的HCAP得率及纯度较高,且主要为ι-卡拉胶。抗病毒活性研究表明HCAP对流感病毒、疱疹病毒和新冠假病毒具有显著的抑制作用,其中对H1N1株Influenza(A/Virginia/ATCC1/2009)的抑制作用最为显著(半抑制浓度IC50=0.03μg/mL,安全指数SI>66666.6)。结论热水提取获得的盾果藻多糖为ι-卡拉胶,其对多种病毒均具有显著的抑制作用,该结果丰富了海洋糖库,为海洋抗病毒药物的开发提供了理论基础。

关键词：盾果藻多糖结构表征抗病毒活性