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Effects of Mitochondrial Dysfunction via AMPK/PGC-1α Signal Pathway on Pathogenic Mechanism of Diabetic Peripheral Neuropathy and the Protective Effects of Chinese Medicine

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Chinese Journal of Integrative Medicine 2019年第5期25卷 386-394页

作者： ZHANG Qian liang xiao-chun Department of Traditional Chinese Medicine Translational Medicine CenterPeking Union Medical College HospitalChinese Academy of Medical SciencesBeijing(100730)China

Diabetic peripheral neuropathy(DPN) is a progressive neurodegenerative disease of peripheral nervous system with high energy requirement. The adenosine monophosphate-activated protein kinase(AMPK)/peroxisome proliferator-activated receptor-γ coactivator 1α(PGC-1α) axis plays a key role in regulating mitochondrial energy metabolism. Increasing preclinical evidences have shown that inhibition of AMPK/PGC-1α pathway leading to mitochondrial dysfunction in neurons or Schwann cells contributes to neuron apoptosis, distal axonopathy and nerve demyelination in DPN. Some Chinese medicine formulae or extracts from herbs may have potential neuroprotective effects on DPN via activating AMPK/PGC-1α pathway and improving mitochondrial function.

关键词： monophosphate-activated protein kinase peroxisome proliferator-activated receptor-γ coactivator 1α sirtuins diabetic peripheral neuropathy Chinese medicine

New Developments in the Pharmacodynamics and Pharmacokinetics of Combination of Chinese Medicine and Western Medicine

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Chinese Journal of Integrative Medicine 2017年第4期23卷 312-319页

作者： LIU Di liang xiao-chun Department of Traditional Chinese Medicine Peking Union Medical College Hospital Peking Union Medical CollegeTranslational Medicine Center Chinese Academy of Medical Sciences Beijing 100730 China

It is very common to use Chinese medicine (CM) combined with Western medicine (WM) in clinical practice. The appropriate combination of CM with WM can reduce toxicity and enhance effects in order to make the best use of advantages and bypass the disadvantages. However, an inappropriate combination can not only affect the curative effect but even cause death. Therefore, strengthening the complementary advantages of the CM and WM to improve the therapeutic efficacy and reduce side effects has become an important research topic of clinical medicine and pharmacy. Many researchers try to clarify the effects of combining CM with WM on therapeutic efficacy and absorption, distribution, metabolism and excretion by pharmacodynamics and pharmacokinetics studies, providing evidence for clinical application. This review focuses on the new developments in the pharmacodynamics and pharmacokinetics of the combination of CM with WM in order to give references for clinical treatment.

关键词： combination of Chinese and Western medicine pharmacodynamics pharmacokinetics

Effects of Hirudin on High Glucose-Induced Oxidative Stress and Inflammatory Pathway in Rat Dorsal Root Ganglion Neurons

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Chinese Journal of Integrative Medicine 2020年第3期26卷 197-204页

作者： LIU Wei liang xiao-chun SHI Yue Department of Traditional Chinese Medicine Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Sciences

Objective:To investigate protective effects of hirudin on oxidative stress and apoptosis of spinal dorsal root ganglion cells in high-glucose rats at the cellular and molecular ***:Dorsal root ganglion neurons(DRGn)were harvested from embryonic day in 15 SD rats,purified and identificated after primary *** were divided into the normal control group,high-glucose(HG)group,positive control(alpha-lipoic acid,ALA)group,low-dose hirudin group(H1),medium-dose hirudin group(H2)and high-dose hirudin group(H3).The control group was cultured by neuron specific culture medium,while the HG group was cultured by neuron specific culture medium and 20 mmol/L glucose(HG medium).The hirudin groups were cultured by HG medium+0.25 IU/mL hirudin(H1),HG medium+0.5 IU/mL hirudin(H2)and HG medium+1 IU/mL hirudin(H3).The ALA group was cultured by HG medium +100μmol/L ALA.3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylt etrazolium bromide(MTT)assay was used to explore the optimum concentration and intervention *** cytometry assay was used to detect the level of reactive oxygen series(ROS).Western blot and quantificational realtime polymerase chain reaction(qRT-PCR)were used to detect the expression of protein and mRNA of nuclear factor erythroid 2-related factor 2(Nrf-2),hemeoxygence-1(HO-1),nuclear factor-κB(NF-κB)and *** assay was used to test the apoptosis rate of different ***:After 24 h of culture,the cell activity of hirudin and ALA groups were higher than that of HG group,and there was a statistical difference between the H1 group and HG group(P<0.05).In hirudin groups,the apoptosis rate of cells,the expression of activated Caspase-3 protein and Caspase-3 mRNA were lower than those of HG group(P<0.01),higher than those of ALA group(P<0.01 or P<0.05).The ROS level of hirudin groups was higher than that of ALA group(P<0.01),lower than that of HG group(P<0.01 or P<0.05).The expression of NF-κB(P65)protein in H3 group were lower than those of HG g

关键词： hirudin diabetic peripheral neuropathy oxidative stress apoptosis dorsal root ganglion neuron

Combination of Quercetin, Hirudin and Cinnamaldehyde Promotes Schwann Cell Differentiation and Myelination against High Glucose by Inhibiting ERK Signaling Pathway

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Chinese Journal of Integrative Medicine 2020年第8期26卷 591-598页

作者： LIU Di liang xiao-chun SUN Ying WU Ya-nan ZHANG Hong Department of Traditional Chinese Medicine Peking Union Medical College HospitalPeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China Department of Cell Resource Center Institute of Basic Medical SciencePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100730China the Department of Nephrology Dongzhimen HospitalBeijing University of Chinese MedicineBeijing 100700China

Objective:To investigate the therapeutic and synergistic effects of QHC(combination of quercetin(Q),hirudin(H)and cinnamaldehyd(C))on Schwann cell differentiation and myelination against high glucose(HG)induced ***:Primary-culture Schwann cells exposed to HG(50 mmol/L)for 72 h and Schwann cell–dorsal root ganglion(DRG)neuron cocultures exposed to HG(50 mmol/L)for 7 days were employed as in vitro model of diabetic *** cells were randomly divided into 10 groups:control(CON,25 mmol/L glucose),HG(50 mmol/L glucose),HG plus 10μmol/L quercetin(Q),HG plus 0.04 IU/mL hirudin(H),HG plus 100 nmol/L cinnamaldehyd(C),HG plus 10μmol/L quercetin and 0.04 IU/mL hirudin(QH),HG plus 10μmol/L quercetin and 50 nmol/L cinnamaldehyd(QC),HG plus 0.04 IU/mL hirudin and 50 nmol/L cinnamaldehyd(HC),HG plus 10μmol/L quercetin,0.04 IU/mL hirudin and 50 nmol/L cinnamaldehyd(QHC)or 10μmol/L *** differentiation was evaluated by periaxin immunofluorescence *** protein expression levels of myelin protein zero(P0),myelin basic protein(MBP),myelin-associated glycoprotein(MAG),extracellular signal-regulated kinase(ERK),p-ERK,p-c-Jun,c-Jun,notch intracellular domain(NICD)and the mRNA expression levels of P0,MBP,MAG,Krox-20,Notch1 and Jagged1 were detected by Western blotting and real-time quantitative PCR *** secretion of ciliary neurotrophic factor(CNTF)was determined by enzyme-linked immunosorbent assay(ELISA).The number and length of the myelin segments were evaluated by MBP immunofluorescence *** expression and the location of p-ERK in cocultures were detected by MAG and p-ERK immunofluorescence double ***:Co-treatment with Q,C,H and their combination promoted Schwann cell differentiation,increased CNTF secretion,up-regulated the protein and m RNA expressions of myelin,and increased the number and length of the myelin segments(P<0.01 or P<0.05).In particular,the combination therapy of Q,H and C was superior to the respective mono

关键词： diabetic peripheral neuropathy extracellular signal-regulated kinase pathway Schwann cells myelination Chinese medicine synergistic effect

Combination of Quercetin, Cinnamaldehyde and Hirudin Protects Rat Dorsal Root Ganglion Neurons against High Glucose-Induced Injury through Nrf-2/HO-1 Activation and NF-κB Inhibition

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Chinese Journal of Integrative Medicine 2017年第9期23卷 663-671页

作者： SHI Yue liang xiao-chun ZHANG Hong SUN Qing WU Qun-li QU Ling Department of Traditional Chinese Medicine Translational Medicine Center Peking Union Medical College HospitalChinese Academy of Medical Sciences Department of Pathology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College

Objective： TO examine the effects of the combination of quercetin （Q）, cinnamaldehyde （C） and hirudin （H）, a Chinese medicine formula on high glucose （HG）-induced apoptosis of cultured dorsal root ganglion （DRG） neurons. Methods： DRG neurons exposed to HG （45 mmol/L） for 24 h were employed as an in vitro model of diabetic neuropathy. Cell viability, reactive oxygen species （ROS） level and apoptosis were determined. The expression of nuclear factor of Kappa B （NF- κB）, inhibitory kappa B α （IκBα ）, phosphorylated IκBα and Nf-E2 related factor 2 （Nrf2） were examined using reverse transcription-polymerase chain reaction （RT-PCR） and Western blot assay. The expression of hemeoxygenase-1 （HO-1）, interleukin-6 （IL-6）, tumor necrosis factor （TNF-α） and caspase-3 were also examined by RT-PCR and Western blot assay. Results： HG treatment markedly increased DRG neuron apoptosis via increasing intracellular ROS level and activating the NF- κB signaling pathway （P〈0.05）. Co-treatment with Q, C, H and their combination decreased HG-induced caspase-3 activation and apoptosis （P〈0.05 or P〈0.01）. The expressions of NF- κB, IL-6 and TNF-α were down- regulated, and Nrf2/HO-1 expression was up-regulated （P〈0.05 or P〈0.01）. QCH has better effect in scavenging ROS, activating Nrf-2/HO-1, and down-regulating the NF- κB pathway than other treatment group. Conclusions： DRG neurons＇ apoptosis was increased in diabetic conditions, which was reduced by QCH formula treatment. The possible reason could be activating Nrf-2/HO-1 pathway, scavenging ROS, and inhibition of NF- κB activation. The effect of QCH combination was better than each monomer or the combination of the two monomers.

关键词： diabetic peripheral neuropathy oxidative stress apoptosis dorsal root ganglion neurons Chinese herb

Selections from Mr. DU xiao-shan's Clinical Experience in Acupuncture

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Journal of Acupuncture and Tuina Science 2003年第6期1卷 3-5页

作者： DU liang-dong xiao Yuan-chun TCM Hospital of Wuxi City Jiangsu China 214001

DU xiao-shan, senior consult doctor, visiting professor of Nanjing University of TCM, tutor of the successor of senior TCM experts' experience, and he is granted the special allowance of State DepartmentMr. DU xiao-shan has been engaged in medical practice for more than sixty years. He has a good command of medical theories and excellent skills of clinical practice. Diagnostically, he combines syndrome differentiation and disease differentiation; therapeutically, he prescribes acupoints and needling techniques in accordance with the etiology and pathogenesis. Especially, he develops and refines some needling techniques on the basis of the classic ones, which are of great value in his clinical practice. I have been following Mr. DU for a long time and achieved certain success. I now present several proven prescriptions below.Author: DU liang-dong (1957-), male, attending physicianTranslator: X{upiao} Yuan-chun

关键词： clinical practice syndrome differentiation

维普期刊数据库评论

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梁晓春治疗耳鸣经验

北京中医药 2021年第4期40卷 391-392页

作者：吴群励杨丹梁晓春(指导) 中国医学科学院北京协和医学院北京协和医院中医科北京100730

耳鸣患病率高,梁晓春教授认为,其发生责之肾、肝、脾三脏,痰、瘀两端,治疗强调补肾为根本、调肝为关键、健脾为枢机,根据兼症,或清火化痰,或燥湿化痰,或祛瘀活血,以开耳窍,方随法定,疗效显著。补肾分阴虚、阳虚,肾阴虚方用耳聋左慈丸,... 详细信息

耳鸣患病率高,梁晓春教授认为,其发生责之肾、肝、脾三脏,痰、瘀两端,治疗强调补肾为根本、调肝为关键、健脾为枢机,根据兼症,或清火化痰,或燥湿化痰,或祛瘀活血,以开耳窍,方随法定,疗效显著。补肾分阴虚、阳虚,肾阴虚方用耳聋左慈丸,常加二至丸或五子衍宗丸;肾阳虚方用金匮肾气丸,常加菟丝子、桑寄生、牛膝、杜仲、补骨脂或益智仁。调肝分气郁、肝火、阴虚及阳亢,气郁方用逍遥散,常加蔓荆子、石菖蒲和香附;肝火方用龙胆泻肝汤;阴虚常加二至丸或枸杞子、桑椹或菊花;阳亢方用天麻钩藤饮,常加龙骨、牡蛎、灵磁石、石决明或珍珠母。健脾分脾胃虚弱及清阳不振,前者方用益气聪明汤,后者方用补中益气汤。

关键词：梁晓春耳鸣补肾调肝健脾化痰祛瘀经验

THE WREATH PRODUCT GROUP FOR NMR SPECTRA ANALYSES OF MOLECULA WITH S_4[S_2]SYMMETRY

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Chinese Chemical Letters 1991年第4期2卷 325-328页

作者： Wei liang ZHOU xiao chun HE Yong Ren HUANG East China Normal University,Shanghai,200062

This paper presents a character table of S_4 wr S_2 wreath product *** this character table,~1H or ^(13)C NMR spectra analysis of molecula with S_4[S_2]symmetry,especially simplification of the secular determinant equation will be easy to carry out. Molecules with S_4[S_2]symmetry,are exemplified by octaphenylcyclo- tetrasiloxane and 2,3,7,8,12,13,17,18-octaethyl-21H,23H-porphine chromium (Ⅲ)chloride.

关键词： NMR UC this THE WREATH PRODUCT GROUP FOR NMR SPECTRA ANALYSES OF MOLECULA WITH S4[S2]SYMMETRY 公寸

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论后现代语境下我国微电影广告的叙事特征

编辑之友 2018年第10期 51-56页

作者：肖小亮何纯湘潭大学文学与新闻学院湖南湘潭411105

广告的故事化叙事倾向和人类对故事的需求使电影媒介成为一种理想的广告载体,加之新技术变革下的"碎片化"时代特征与商品化逻辑促使微电影广告的生成。后现代文化同样也是技术变革的产物,深深地打上了商品化逻辑的烙印。微电影广告叙事... 详细信息

广告的故事化叙事倾向和人类对故事的需求使电影媒介成为一种理想的广告载体,加之新技术变革下的"碎片化"时代特征与商品化逻辑促使微电影广告的生成。后现代文化同样也是技术变革的产物,深深地打上了商品化逻辑的烙印。微电影广告叙事承载着后现代主义的文化倾向和精神内涵,呈现出意义浅表化、情节拼贴化、话语多元化特征。这些特征在一定程度上满足了人们的感性需求,但对感性需求的过度满足则会导致人的社会批判意识的丧失。

关键词：微电影广告叙事后现代语境