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Effects of drought on non-structural carbohydrates and C,N,and P stoichiometric characteristics of Pinus yunnanensis seedlings

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Journal of Forestry Research 2024年第1期35卷 90-102页

作者： Zhijuan Zhao lina Wang Yuanxi liu Jianli Sun Jiandong Xiao Qiong Dong lianfang li Wanjie Zhang Chao Wang Junwen Wu College of Forestry Southwest Forestry UniversityKunming 650224YunnanPeople’s Republic of China

To study non-structural carbohydrate character-istics and nutrient utilization strategies of Pinus yunnanen-sis under continuous drought conditions,2-year-old seed-lings were planted in pots with appropriate water,light and moderate and severe drought treatments[(80±5),(65±5),(50±5),and(35±5)%of field water-holding capacity].Non-structural carbohydrates,carbon(C),nitrogen(N),and phosphorus(P)concentrations were measured in each plant component.The results show that:(1)With increasing drought,non-structural carbohydrates gradually increased in leaves,stems,and coarse roots,while gradually decreased in fine roots;(2)C concentrations of all were relatively stable under different stress levels.Phosphorous utilization of each component increased under light and moderate drought conditions,while N and P utilization efficiency of each plant component decreased under severe drought.Growth was mainly restricted by N,first decreasing and then increasing with increased drought;(3)There was a correlation between the levels of non-structural carbohydrates and C,N,and P in each component.Changes in N concentration affected the interconversion between soluble sugar and starch,which play a regulatory role in the fluctuation of the concentration of non-structural carbohydrates;and,(4)Plasticity analysis showed that P.yunnanensis seedlings responded to drought mainly by altering starch concentration,the ratio of soluble sugar to starch in leaves and stems,and further by alter-ing N and P utilization efficiencies.Overall,these results suggest that the physiological activities of all organs of P.yunnanensis seedlings are restricted under drought and that trade-offs exist between different physiological indicators and organs.Our findings are helpful in understanding non-structural carbohydrate and nutrient adaptation mechanisms under drought in P.yunnanensis seedlings.

关键词： Pinus yunnanensis seedlings Drought Non-structural carbohydrates C,N,P stoichiometric characteristics

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Formulation of Work-Study Combined and Result-Oriented Integrated Curriculum Standards

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Medicinal Plant 2024年第3期15卷 79-83页

作者： lianfang li Chunhua DU Fen YANG Yun li Yanfei NIU Yunnan Technician College(Yunnan Industry Trade Vocational College) Kunming 650300China Yunnan Baiyao Group Traditional Chinese Medicine Resources Co. Ltd.Kunming 650300China

According to the Annex Technical Regulations for Integrated Curriculum Development(Trial)in Document No.30 of the General Office of the Ministry of Human Resources and Social Security(2012),this paper studies the formulation of the curriculum standards for the integration of Chinese medicinal materials production.We focus on the formulation ideas of the curriculum standards for the integration of Chinese medicinal materials production,the formulation process of the curriculum standards for the integration of Chinese medicinal materials production,including the description of typical work tasks,the determination of curriculum objectives,the analysis of study content,the description of referential study tasks,teaching implementation suggestions,assessment and evaluation suggestions,which can provide a reference for the development and research of other related integrated courses.

关键词： Integration of work and study Work process Curriculum standards Production of Chinese medicinal materials Typical work tasks Referential study tasks

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Comparison of Different Processing Methods of Ziziphus jujuba Mill.

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Medicinal Plant 2021年第1期12卷 43-46页

作者： lianfang li Yunnan Industry&Trade Vocational College Kunming 650300China

[Objectives]To study and compare the processing methods of Ziziphus jujuba Mill.[Methods]Z.jujuba was processed by seven methods:cleaning,yellowish frying,burnt frying,carbonized frying,moistening by hot sand,steaming,and boiling.Ultraviolet-visible spectrophotometry was used to determine the content of total flavonoids in each processed Z.jujuba.[Results]The content of total flavonoids measured by various processing methods was different.The content of total flavonoids in the processed Z.jujuba by moistening by hot sand was relatively high and the absorption peak spectrum was almost the same as that of the cleaned processed Z.jujuba and the rutin reference substance.[Conclusions]The moistening by hot sand is the optimal method for processing of Z.jujuba.

关键词： Ziziphus jujuba Mill. Processing of traditional Chinese medicine Total flavonoids Ultraviolet-visible spectrophotometry

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Review of Processing Methods of Jujube

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Medicinal Plant 2021年第4期12卷 93-95,97页

作者： lianfang li Yunnan Industry & Trade Vocational College Kunming 650300 China

Historical evolution of jujube processing,recording situation of modern processing specifications of jujube,research status of processing methods of jujube at home in recent years,content analysis of main chemical composition in jujube processed products,and impact of processing on chemical composition of jujube are reviewed,which could provide basic data for fully studying,developing and utilizing jujube by scholars and technical staff.

关键词： Jujube Processing method Chemical constituents

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超声下乳腺影像报告和数据系统3类结节微创旋切术后确诊乳腺癌患者临床病理学特征及肿瘤残留影响因素分析

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中华外科杂志 2024年第2期62卷 135-140页

作者：李亮冯泽群张连芳王瑞卿张小霞刘丽媛于理想余之刚高忠诚山东大学第二医院乳腺外科、山东大学乳腺疾病防治转化医学研究所、山东省乳腺疾病防治转化工程实验室济南250033 临沂市人民医院影像科临沂276000 临沂市人民医院乳腺外科临沂276000

目的探讨超声下乳腺影像报告和数据系统(BI-RADS)3类结节微创旋切术后确诊乳腺癌患者的临床病理学特征及肿瘤残留的影响因素。方法本研究为回顾性病例对照研究。回顾性收集2012年5月至2016年6月就诊于山东大学第二医院乳腺外科、临沂市... 详细信息

目的探讨超声下乳腺影像报告和数据系统(BI-RADS)3类结节微创旋切术后确诊乳腺癌患者的临床病理学特征及肿瘤残留的影响因素。方法本研究为回顾性病例对照研究。回顾性收集2012年5月至2016年6月就诊于山东大学第二医院乳腺外科、临沂市人民医院乳腺外科的4151例乳腺超声BI-RADS 3类结节微创旋切术中确诊乳腺癌的69例患者的临床病理学资料,患者均为女性,年龄(43.4±8.2)岁(范围:22~70岁)。根据微创旋切术后有无肿瘤残留分为残留组和无残留组,采用χ^(2)检验、独立样本t检验比较两组患者的临床病理学资料。采用Logistic回归分析肿瘤残留的影响因素,应用Kaplan-Meier法进行生存分析并绘制生存曲线。结果乳腺超声BI-RADS 3类结节微创旋切术后确诊乳腺癌的患者占同时期该类患者的1.66%(69/4151)。其肿物最大径为(1.27±0.45)cm(范围:0.5~2.3 cm),28例肿物最大径≤1 cm,41例肿物最大径>1 cm,组织病理学结果为原位癌24例,浸润性癌41例,雌激素受体阳性47例,孕激素受体阳性43例,Ki-67指数升高26例。腋窝转移阳性率10.1%(7/69)。39例患者存在肿瘤残留,占乳腺癌患者的56.5%(39/69)。单因素分析结果显示,残留组雌激素受体阳性比例更高[91.9%(34/37)比61.9%(13/21),χ^(2)=7.838,P=0.012]。多因素分析结果显示,雌激素受体阳性(OR=16.852,95%CI:1.819~156.130,P=0.013)为术后肿瘤残留的独立影响因素。乳腺超声BI-RADS 3类结节乳腺癌患者5年无病生存率为97.1%,总体生存率为98.6%。结论可用微创旋切术处理的BI-RADS 3类结节具有一定恶性风险,约为1.66%。雌激素受体阳性的患者更易发生肿瘤残留。微创旋切术后存在一定的肿瘤残留,对确诊为乳腺癌患者,需进行二次手术确保残腔无肿瘤残留。

关键词：乳腺肿瘤活组织检查微创旋切术肿瘤残留预后

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高阿丁枫人工中龄林生长对坡向和密度的响应

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西南林业大学学报（自然科学） 2024年第2期44卷 42-51页

作者：李小军王慷林李莲芳张合瑶周冬梅刘娴顾梦王文俊侯海雄西南林业大学云南昆明650233

为了解高阿丁枫人工林的生长过程,2021年对林龄21 a的高阿丁枫人工林进行每木检尺,以胸径作为标准木选择的依据,西南坡不同密度中各选择1株,南坡不同密度中各选择3株高阿丁枫标准木作为解析木,分析不同坡向和造林密度组合的林木生长差... 详细信息

为了解高阿丁枫人工林的生长过程,2021年对林龄21 a的高阿丁枫人工林进行每木检尺,以胸径作为标准木选择的依据,西南坡不同密度中各选择1株,南坡不同密度中各选择3株高阿丁枫标准木作为解析木,分析不同坡向和造林密度组合的林木生长差异。结果表明:林龄21 a时,不同坡向和造林密度组合的高阿丁枫林木的平均胸径、树高和材积分别为17.60~20.78 cm、25.73~28.51 m和0.3186~0.4293 m3,林木尚处于旺盛的生长阶段;处理组合间以上生长量均呈现极显著的差异。林龄1~9、1~10、8~16 a期间分别是胸径、树高和材积的速生期,其连年生长量最高分别达2.19 cm/a、2.74 m/a和4.3966×10^(-2)m^(3)/a。坡向、密度及其交互作用显著或极显著地影响高阿丁枫林木的生长。高阿丁枫隶属热区长寿命大径材树种,速生期延续较长,其生长对立地和密度较为敏感。优质高效的高阿丁枫人工林培育应选择最适宜的立地和造林密度,并且适时开展密度的动态调控。综上所述,造林密度通过影响树高生长从而影响材积生长,南坡采用3 m×2 m×5 m非均匀密度造林的单株总材积最大。研究结果可应用于生产实践中高阿丁枫优质高效人工林培育的立地选择和密度控制。

关键词：高阿丁枫裂区设计坡向密度林木生长

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优质抗逆有棱丝瓜新品种夏绿5号的选育

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长江蔬菜 2024年第8期 43-46页

作者：朱德宁吴宇军曹翠文林鉴荣李莲芳广州市农业科学研究院广东广州510000

夏绿5号是有棱丝瓜杂种一代新品种。长绿类型丝瓜,早熟,生长势较强,分枝性较强,播种至开花春季32~38 d,秋季36~40 d;播种到初收春季40~48 d,秋季44~47 d;第一瓜节位春季7.0~14.0节,秋季21.0~25.0节;瓜长50.0~55.0 cm,横径4.8~5.3 cm,单... 详细信息

夏绿5号是有棱丝瓜杂种一代新品种。长绿类型丝瓜,早熟,生长势较强,分枝性较强,播种至开花春季32~38 d,秋季36~40 d;播种到初收春季40~48 d,秋季44~47 d;第一瓜节位春季7.0~14.0节,秋季21.0~25.0节;瓜长50.0~55.0 cm,横径4.8~5.3 cm,单瓜质量450.0~550.0 g,棱沟浅;果色深绿,有光泽,无花点,棱角墨绿色;瓜条匀称,肉质紧实,口感脆,味微甜,品质优,含维生素C 10.9 mg/100 g,粗纤维0.7 g/100 g,可溶性固形物6.6 g/100 g,蛋白质1.1 g/100 g。以主蔓结果为主,连续坐果能力较强,耐贮运,产量高,一般产量在1 500~3 000 kg/667 m2,耐热性强,抗病性较强,商品性状优良。适合华南丝瓜产区夏秋季种植。

关键词：有棱丝瓜杂交新品种选育维生素C

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中国输血杂志 2024年第1期37卷 43-50页

作者：谢桂芸黎世杰欧阳剑冯凡凡郑晓晓周芷羽麦连芳陈锦艳广州血液中心广州市血液安全重点实验室广东广州510080

目的对比3种献血前补液方式对预防即发型及迟发型献血相关血管迷走神经反应(vasovagal response,VVR)的效果。方法2021年1—6月,在本中心6个固定献血点对符合献血条件,签署知情同意的6250名全血献血者,按献血点和日期分为198个群组,按... 详细信息

目的对比3种献血前补液方式对预防即发型及迟发型献血相关血管迷走神经反应(vasovagal response,VVR)的效果。方法2021年1—6月,在本中心6个固定献血点对符合献血条件,签署知情同意的6250名全血献血者,按献血点和日期分为198个群组,按随机数法随机分配到口服补液盐(ORS)组,糖水组或饮用水组,分别在献血前20 min内喝完500 mL饮用水,糖水及ORS,研究人员现场记录干预的实际完成情况,并登记现场发生的VVR及相关情况。献血后休息时,献血者完成包含社会人口学信息的电子问卷。献血后48 h,研究人员电话回访每位献血者,登记迟发型VVR及相关情况。基于意向性分析法(ITT)Logistic回归分析3组间VVR发生率差异,考虑到依从性的影响,计算干预组的平均处理效应(ATT)。用PASS 2021估算样本量,R(4.2.0)做统计分析。结果本中心固定点全血献血者在3种不同的献血前补液方式干预下,献血相关VVR的累计发生率为2.67%(2.29%~3.11%),其中,即发型和迟发型VVR的发生率分别为1.02%(0.79%~1.31%)和1.65%(1.36%~2.01%)。ITT分析仅发现ORS比水组可有效降低迟发型VVR发生率【OR=0.59,95%CI[0.37,0.94]】。ATT分析考虑了依从性的影响发现:任意两组间即发型VVR发生率差异均无统计学意义(P>0.05);糖水组对比饮用水组的迟发型VVR发生率差异无统计学意义(P>0.05)。补充ORS对比饮用水,迟发型VVR发生率平均下降值相差了-0.013,95%CI[-0.022,-0.004],补充ORS对比糖水,迟发型VVR发生率平均下降值相差了-0.008,95%CI[-0.017,-0.000],差异均有统计学意义(P<0.05)。3个组累计VVR显示了与迟发型VVR相似的结果。结论献血前补充ORS对预防迟发型VVR效果最好,下一步可建立迟发型VVR预测模型甄别易感人群,建议其献血前喝ORS,其他人群献血前可根据喜好任选液体,实现献血相关VVR的个性化防控。

关键词：献血相关血管迷走神经反应群组随机试验逻辑回归平均处理效应(ATT)

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Ultrasound-enhanced fluorescence imaging and chemotherapy of multidrugresistant tumors using multifunctional dendrimer/carbon dot nanohybrids

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Bioactive Materials 2021年第3期6卷 729-739页

作者： Dan li lizhou lin Yu Fan Long liu Mingwu Shen Rong Wu lianfang Du Xiangyang Shi Department of Interventional and Vascular Surgery Shanghai Tenth People's HospitalTongji University School of MedicineShanghai200072People's Republic of China Department of Ultrasound Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai200080People's Republic of China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201620People's Republic of China

Development of innovative nanomedicine enabling enhanced theranostics of multidrug-resistant(MDR)tumors remains to be challenging.Herein,we report the development of a newly designed multifunctional yellowfluorescent carbon dot(y-CD)/dendrimer nanohybrids as a platform for ultrasound(US)-enhanced fluorescence imaging and chemotherapy of MDR tumors.Generation 5(G5)poly(amidoamine)dendrimers covalently modified with efflux inhibitor of D-α-tocopheryl polyethylene glycol 1000 succinate(G5-TPGS)were complexed with one-step hydrothermally synthesized y-CDs via electrostatic interaction.The formed G5-TPGS@y-CDs complexes were then physically loaded with anticancer drug doxorubicin(DOX)to generate(G5-TPGS@y-CDs)-DOX complexes.The developed nanohybrids display a high drug loading efficiency(40.7%),strong y-CD-induced fluorescence emission,and tumor microenvironment pH-preferred DOX release profile.Attributing to the DOX/TPGS dual drug design,the(G5-TPGS@y-CDs)-DOX complexes can overcome the multidrug resistance(MDR)of cancer cells and effectively inhibit the growth of cancer cells and tumors.Furthermore,the introduction of US-targeted microbubble destruction technology was proven to render the complexes with enhanced intracellular uptake and anticancer efficacy in vitro and improved chemotherapeutic efficacy and fluorescence imaging of tumors in vivo due to the produced sonoporation effect.The developed multifunctional dendrimer/CD nanohybrids may represent an advanced design of nanomedicine for US-enhanced theranostics of different types of MDR tumors.

关键词： Dendrimers Carbon dots Multidrug-resistant tumors Ultrasound-targeted microbubble destruction technology Fluorescence imaging Chemotherapy

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Response of phytoplankton community to different water types in the western Arctic Ocean surface water based on pigment analysis in summer 2008

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Acta Oceanologica Sinica 2017年第8期36卷 109-121页

作者： JIN Haiyan ZHUANG Yanpei li Hongliang CHEN lianfang GAO Shengquan JI Zhongqiang ZHANG Yang Laboratory of Marine Ecosystem and Biogeochemistry of SOA Second Institute of OceanographyState Oceanic Administration(SOA)Hangzhou 310012China State Key Laboratory of Satellite Ocean Environment Dynamics Hangzhou 310012China Alfred Wegener Institute Helmholtz Centre for Polar and Marine ResearchBremerhaven 27568Germany

Nutrients and photosynthesis pigments were investigated in the western Arctic Ocean during the 3rd Chinese Arctic Research Expedition Cruise in summer 2008. The study area was divided into five provinces using the K- means clustering method based on the physical and chemical characteristics of the sea water, and to discuss the distribution of the phytoplankton community structure in these provinces. CHEMTAX software was performed using HPLC pigments to estimate the contributions of eight algal classes to the total chlorophyll a （TChl a）. The results showed that on the Chukchi Shelf, the Pacific Ocean inflow mainly controlled the Chl a biomass and phytoplankton communities by nutrient concentrations. The high nutrient Anadyr Water and Bering Shelf Water （AnW and BSW） controlled region have high Chl a levels and the diatom dominated community structure. In contrast, in the region occupied by low-nutrient like Alaska Coastal Water （ACW）, the Chl a biomass was low, with pico- and nano-phytoplankton as dominated species, such as prasinophytes, chrysophytes and cryptophytes. However, over the off-shelf, the ice cover condition which would affect the physical and nutrient concentrations of the water masses, in consequence had a greater impact on the phytoplankton community structure. Diatom dominated in ice cover region and its contribution to Chl a biomass was up to 75%. In the region dose to the Mendeleev Abyssal Plain （MAP）, controlled by sea-ice melt water with relatively high salinity （MW-HS）, higher nutrient and Chl a concentrations were found and the phytoplankton was dominated by pico- and nano-algae, while the diatom abundance reduced to 33%. In the southern Canada Basin, an ice-free basin （IfB） with the lowest nutrient concentrations and most freshened surface water, low Chl a biomass was a consequence of low nutrients. The ice retreating and a prolonged period of open ocean may not be beneficial to the carbon export efficiency due to reducing the Chl a biomass or intriguing smaller size algae growth.

关键词： photosynthetic pigments phytoplankton community biological pump organic carbon ice retreat Chukchi Sea and Canada Basin

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