检索结果-南通市图书馆

Dynamic Intra-Japonica Subspecies Variation and Resource Application

同方学位论文库评论

在线全文

同方学位论文库

学校读者我要写书评

暂无评论

Molecular Plant 2012年第1期5卷 218-230页

作者： Haiyan Lin Peng Xia rod A.Wing Qifa Zhang Meizhong Luo National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Arizona Genomics Institute University of ArizonaTucsonAZ 85721USA

We constructed a physical map of O. sativa ssp. japonica cv. ZH11 and compared it and its random sample sequences with the Nipponbare RefSeq derived from the same subspecies. This comparison showed that the two japonica genomes were highly syntenic but revealed substantial differences in terms of structural variations, rates of substitutions and indels, and transposable element content. For example, contractions/expansions as large as 450 kb and repeat sequences that were present in high copy numbers only in ZH11 were detected. In tri-alignment regions using the indica variety 93-11 sequence as an outgroup, we found that：（1） the substitution rates of the two japonica-indica inter- subspecies comparison combinations were close but almost a magnitude higher than the substitution rate between the japonica rice varieties ZH11 and Nipponbare; （2） of the substitutions found between ZH11 and Nipponbare, 47.2% occurred in ZH11 and 52.6% in Nipponbare; （3） of the indels found between ZH11 and Nipponbare, the indels that occurred in ZH11 were 15.8 times of those in Nipponbare. Of the indels that occurred in ZH11, 75.67% were insertions and 24.33% deletions. Of the indels that occurred in Nipponbare, 48.23% were insertions and 51.77% were deletions. The ZH11 com- parative map covered four Nipponbare physical gaps, detected assembly errors in the Nipponbare sequence, and was integrated with the FSTs of a large ZH11 T-DNA insertion mutant library. ZH11 BAC clones can be browsed, searched, and obtained at our website, http：//***.

关键词： Physical map comparative genomics Japonica Oryza rice BAC.

The Subtelomere of Oryza sativa Chromosome 3 Short Arm as a Hot Bed of New Gene Origination in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2008年第5期1卷 839-850页

作者： Chuanzhu Fana Yong Zhang Yeisoo Yu Steve Rounsley Manyuan Long rod A. Wing Arizona Genomics Institute Department of Plant Sciences University of Arizona Tucson AZ 85721 USA Department of Ecology and Evolution University of Chicago Chicago IL 60637 USA BIO5 Institute for Collaborative Research University of Arizona Tucson AZ 85721 USA

Despite general observations of non-random genomic distribution of new genes, it is unclear whether or not new genes preferentially occur in certain genomic regions driven by related molecular mechanisms. Using 1.5 Mb of genomic sequences from short arms of chromosome 3 of Oryza glaberrima and O. punctata, we conducted a comparative genomic analysis with the reference O. sativa ssp. japonica genome. We identified a 60-kb segment located in the middle of the subtelomeric region of chromosome 3, which is unique to the species O. sativa. The region contained gene duplicates that occurred in Asian cultivated rice species that diverged from the ancestor of Asian and African cultivated rice one million years ago （MYA）. For the 12 genes and one complete retrotransposon identified in this segment in O. sativa ssp. japonica, we searched for their parental genes. The high similarity between duplicated paralogs further supports the recent origination of these genes. We found that this segment was recently generated through multiple independent gene recombination and transposon insertion events. Among the 12 genes, we found that five had chimeric gene structures derived from multiple parental genes. Nine out of the 12 new genes seem to be functional, as suggested by Ka/Ks analysis and the presence of cDNA and/or MPSS data. Furthermore, for the eight transcribed genes, at least two genes could be classified as defense or stress response-related genes. Given these findings, and the fact that subtelomeres are associated with high rates of recombination and transcription, it is likely that subtelomeres may facilitate gene recombination and transposon insertions and serve as hot spots for new gene origination in rice genomes.

关键词： comparative genomics gene duplication Oryza sativa subtelomere new genes.

Characterization of seedling proteomes and development of markers to distinguish the Brassica A and C genomes

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2010年第5期37卷 333-340页

作者： Fang Kong Cailin Ge Xiaoping Fang rod J. Snowdon Youping Wang College of Bioscience and Biotechnology Yangzhou University Yangzhou 225009 China Oil Crops Research Institute Chinese Academy of Agricultural Sciences Wuhan 430062 China Institute for Plant Breeding and Crop Science I Justus Liebig University Heinrich-Buff-Ring 26-32 Giessen 35392 Germany

The diploid species Brassica rapa （genome AA） and B. oleracea （genome CC） were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins （32.0%） and specific proteins （39.8%） of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.

关键词： Brassica rapa Brassica oleracea proteomics molecular marker

Anti-Toxocara *** in an adult healthy population:serosurvey and risk factors in Southeast Brazil

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2013年第3期3卷 211-216页

作者： Elaine Cristina Negri Vamilton alvares Santarem Guita Rubinsky-Elefant Rogerio Giuffrida Pro-Reitoria de Pesquisa e Pos-GraduacaoRod.Raposo Tavares km 572Bairro Limoeiro19067-175 Presidente PrudenteSao PauloBrazil Laboratory of Preventive Veterinary Medicine Veterinary Teaching-Hospital-Unoeste-Rod.Raposo Tavares km 572Bairro Linmeiro19067-175 Presidente PrudenteSao Paulo.Brazil Laboratory of Seroepidemiology and Immunobiology-Instituto de Medicina Tropical de Sao Paulo-University of Sao Paulo Av Dr Eneas deCarralho Aguiar 47005403-000 Sao PauloSPBrazil Laboratory of Preventive Veterinary Medicine Veterinary Teaching-Hospital-Unoeste-Rod.Raposo Tavares km 572Bairro Linmeiro19067-175Presidente PrudenteSao Paulo.Brazil

Objective:To evaluate the frequency of anti-Toxocara *** in an adult healthy ***:The study was performed by interviewing 253 blood donors,from 19 to65 years of age,in a hematological centre in Presidente Prudente,S(?)o Paulo,southeast Brazil.A survey was applied to blood donors in order to evaluate the possible factors associated to the presence of antibodies,including individual(gender and age),socioeconomic(scholarship,familial income and sanitary facilities)and habit information(contact with soil,geophagy,onycophagy and intake of raw/undercooked meat)as well as the presence of dogs or cats in the *** test was run for detection of the anti-Toxocara *** *** analysis followed by logistic regression was performed to evaluate the potential risk factors associated to ***:The overall prevalence observed in this study was 8.7%(22/253).Contact with soil was the unique risk factor associated with the presence of antibodies(P=0.0178;0R=3.52;95%CI=1.244-9.995).Conclusions:The results of this study reinforce the necessity in promoting preventive public health measures,even for healthy adult individual,particularly those related to the deworming of pets to avoid the soil contamination,and hygiene education of the population.

关键词： Toxocariasis Larva migrans Seroprevalence Diagnostic Epidemiology

Pediatric interfacility transport effects on mortality and length of stay

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

作者： rod Phillips.

学校读者我要写书评

暂无评论

World Journal of Pediatrics 2021年第4期17卷 400-408页

作者： rod M.Shinozaki Andreas Schwingshackl Neeraj Srivastava Tristan Grogan Robert B.Kelly David Geffen School of Medicine at UCLA Los AngelesCAUSA University of California IrvineIrvineCAUSA Children’s Hospital of Orange County 1201 West La Veta AvenueOrangeCA92868USA

Background We aimed to evaluate the effects of interfacility pediatric critical care transport response time,physician presence during transport,and mode of transport on mortality and length of stay(LOS)among pediatric *** hypothesized that a shorter response time and helicopter transports,but not physician presence,are associated with lower mortality and a shorter *** Retrospective,single-center,cohort study of 841 patients(<19 years)transported to a quaternary pediatric intensive care unit and cardiovascular intensive care unit between 2014 and 2018 utilizing patient charts and transport *** linear and logistic regression analyses adjusted for age,diagnosis,mode of transport,response time,stabilization time,return duration,mortality risk(pediatric index of mortality-2 and pediatric risk of mortality-3),and inotrope,vasopressor,or mechanical ventilation presence on *** Four hundred and twenty-eight(50.9%)patients were transported by helicopter,and 413(49.1%)were transported by *** accompanied 239(28.4%)*** median response time was 2.0(interquartile range 1.4–2.9)*** physician presence increased the median response time by 0.26 hours(P=0.020),neither physician presence nor response time significantly affected mortality,ICU length of stay(ILOS)or hospital length of stay(HLOS).Helicopter transports were not significantly associated with mortality or ILOS,but were associated with a longer HLOS(3.24 days,95%confidence interval 0.59–5.90)than ambulance transports(P=0.017).Conclusions These results suggest response time and physician presence do not significantly affect mortality or *** may reflect the quality of pre-transport care and medical control *** transports were only associated with a longer *** analysis provides a framework for examining transport workforce needs and associated costs.

关键词： Helicopter Hospital length of stay Pediatric critical care Pediatric intensive care unit Transport medicine

Anatomy & physiology / 5th ed. 5th ed.

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Ortholog Alleles at Xa3/Xa26 Locus Confer Conserved Race-Specific Resistance against Xanthomonas oryzae in Rice

2000年

作者： Seeley rod R.

来源：畅想之星图书光盘版（南... 评论

在线全文

畅想之星图书光盘版（南通市图书馆镜像）

学校读者我要写书评

暂无评论

Molecular Plant 2012年第1期5卷 281-290页

作者： Hong-Jing Li Xiang-Hua Li Jing-Hua Xiao rod A.Wing Shi-Ping Wang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWuhan 430070China Arizona Genomics Institute School of Plant SciencesBIO5 InstituteUniversity of ArizonaTucsonAZ 85721USA

The rice disease resistance （R） gene Xa3/Xa26 （having also been named Xa3 and Xa26） against Xanthomonas oryzae pv. oryzae （Xoo）, which causes bacterial blight disease, belongs to a multiple gene family clustered in chromosome 11 and is from an AA genome rice cultivar （Oryza sativa L.）. This family encodes leucine-rich repeat （LRR） receptor kinase- type proteins, Here, we show that the orthologs （alleles） of Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3, from wild Oryza spe- cies O. officinalis （CC genome） and O. minuta （BBCC genome）, respectively, were also R genes against Xoo. Xa3/Xa26-2 and Xa3/Xa26-3 conferred resistance to 16 of the 18 Xoo strains examined. Comparative sequence analysis of the Xa3/Xa26 families in the two wild Oryza species showed that Xa3/Xa26-3 appeared to have originated from the CC genome of O. minuta. The predicted proteins encoded by Xa3/Xa26, Xa3/Xa26-2, and Xa3/Xa26-3 share 91-99% sequence identity and 94-99% sequence similarity. Transgenic plants carrying a single copy of Xa3/Xa26, Xa3/Xa26-2, or Xa3PXa26-3, in the same genetic background, showed a similar resistance spectrum to a set of Xoo strains, although plants carrying Xa3/Xa26-2 or Xa3/Xa26-3 showed lower resistance levels than the plants carrying Xa3/Xa26. These results suggest that the Xa3/Xa26 locus predates the speciation of A and C genome, which is approximately 7.5 million years ago. Thus, the resistance spec- ificity of this locus has been conserved for a long time.

关键词： Bacterial blight broad-spectrum resistance durable resistance Oryza officinalis Oryza minuta Oryza sativa.

Rice Gene Index:A comprehensive pan-genome database for comparative and functional genomics of Asian rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2023年第5期16卷 798-801页

作者：于志超陈永明周勇张雨露李梦圆欧阳亦聃 Dmytro Chebotarov Ramil Mauleon 赵虎谢为博 Kenneth L.McNally rod A.Wing 郭伟龙张建伟 National Key Laboratory of Crop Genetic Improvement Hubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China Frontiers Science Center for Molecular Design Breeding Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic ImprovementChina Agricultural UniversityBeijing 100193China Center for Desert Agriculture Biological and Environmental Sciences&Engineering Division(BESE)King Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Saudi Arabia International Rice Research Institute(IRRI) Los Ban˜osLaguna 4031Philippines Arizona Genomics Institute School of Plant SciencesUniversity of ArizonaTucsonAZ 85721USA

Dear Editor,Asian rice(Oryza sativa)is the staple food for half the world and is a model crop that has been extensively *** contributes20%of calories to the human diet(Stein et al.,2018).With the increase in global population and rapid changes in climate,rice breeders need to develop new and sustainable cultivars with higher yields,healthier grains,and reduced environmental footprints(Wing et al.,2018).Since the first gold-standard reference genome of rice variety Nipponbare was published(International Rice Genome Sequencing Project,2005),an increasing number of rice accessions have been sequenced,assembled,and annotated with global ***,a single reference genome is obviously insufficient to perform the genetic difference analysis for rice ***,the pan-genome has been proposed as a solution,which allows the discovery of more presence-absence variants compared with single-reference genome-based studies(Zhao et al.,2018).Over the past years,several databases,such as RAP-db(https://***),RGAP(http://***),and Gramene(https://***),have long-term served rice genomic research by providing information based on one or a series of individual reference *** integrate and utilize the genomic information of multiple accessions,we performed comparative analyses and established the user-friendly Rice Gene Index(RGI;https://***)*** is the first gene-based pan-genome database for rice.

关键词： sativa comprehensive cultivar