检索结果-南通市图书馆

Anti-sense oligonucleotide labeled with technetium-99m using hydrazinonictinamide derivative and N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycline: A comparison of radiochemical behaviors and biological properties

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2008年第14期14卷 2235-2240页

作者： Yun-Chun li tian-zhi Tan Jian-Guo Zheng Chun Zhang Department of Nuclear Medicine West China Hospital Sichuan University 37 Guoxue Alley Chengdu 610041 Sichuan Province China

AIM:To explore and compare the radiochemical behavior and biological property of anti-sense oligonuc-leotide (ASON) labeled with technetium-99m using N-hydroxysuccinimidyl S-acetylmercaptoacetyltriglycl ine (NHS-MAG3) and hydrazinonictinamide derivative (HYNIC). METHODS:After HYNIC and NHS-MAG3 were synthesized, ASON was labeled with technetium-99m using HYNIC and NHS-MAG3 as a bifunctional chelator. The in vivo and in vitro stability, binding rates of labeled compounds to serum albumen, biodistribution of 99mTc-MAG3-ASON and 99mTc-HYNIC-ASON in BALB/C mouse and its HT29 tumor cellular uptake were compared. RESULTS:The labeling efficiency and stability of 99mTc-MAG3-ASON were significantly higher than those of 99mTc-HYNIC-ASON (P = 0.02, and P = 0.03, respectively). 99mTc-MAG3-ASON had a significantly lower rate of binding to serum albumen than 99mTc-HYNIC-ASON (P liver and stomach (P lightly lower in intestines and spleen (P > 0.05) and significantly higher in lung and kidney (P lication.

关键词： Anti-sense oligonucleotide Radiolabeling Technetium-99m N-hydroxysuccinimidyl S-acetylmercapt oacetyltriglycline Hydrazinonictionamide derivative

Effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2013年第2期26卷 118-127页

作者： li tian zhi YUAN Ming CHEN Zhen Hong GUO Ying Hua KANG Chun Yan WANG Jing Yu li zhi li WANG De Sheng WANG Hui Juan YUAN Min liU Chang Ting Nanlou Respiratory Diseases Department Chinese PLA General Hospital State KeyLaboratory of Space Medicine Fundamentals and Application China Astronaut Research and Training Center

Objective To study the effect of Simulated Microgravity and its Associated Mechanism on Pulmonary Circulation in Rats. Methods Rat tail-suspension model was used to simulate the physiological effects of microgravity and changes in pulmonary blood vessel morphology, pulmonary arterial and venous blood pressure, pulmonary vascular resistance, pulmonary vasomotoricity, as well as the regulation of pulmonary circulation by cytokines produced and released by the lung of rats were measured. Results The walls of pulmonary blood vessels of rats were thickened, and the pulmonary artery was reconstructed with increased pulmonary vascular resistance. The pulmonary blood vessels of rats became more prone to dilation as contractions increased. Rat epithelial Adrenomedulin gene transcription and protein expression were upregulated. The level of basic fibroblast growth Factor of rat was also elevated. Conclusion Findings from the present study on rats revealed that the microgravity can affect pulmonary blood vessel structure, pulmonary arterial pressure, and pulmonary blood vessel self-regulation and cytokine production.

关键词： Rat Simulated microgravity Changes in pulmonary circulation Regulations of pulmonarycirculation

A homolog of glyceraldehyde-3-phosphate dehydrogenase from Riemerella anatipestifer is an extracellular protein and exhibits biological activity

在线全文

学校读者我要写书评

暂无评论

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第9期15卷 776-787页

作者： Ji-ye GAO Cui-lian YE li-li ZHU zhi-ying tian zhi-bang YANG Department of Basic Medicine Chongqing Medical University Chongqing 400016 China

Riemerella anatipestifer is the causative agent of septicemia anserum exsudativa in ducks. Its pathogenesis and virulence factors are still unclear. The glycelytic enzyme, glyceraldehyde-3-phosphate dehydrogenase （GAPDH）, an anchorless and multifunctional protein on the surface of several pathogenic microorganisms, is involved in virulence and adhesion. Whether homologs of GAPDH exist, and display similar characteristics in R. anatipestifer （RaGAPDH） has not been determined. In our research, the RaGAPDH activity from various R. anatipestifer isolates was confirmed. Twenty-two gapdh genes from genornic DNA of R. anatipestifer isolates were cloned and sequenced for phylogenetic analysis. The distribution of RaGAPDH in R. anatipestifer CZ2 strain was confirmed by antisera to recombinant RaGAPDH. The ability of purified RaGAPDH to bind host proteins was analyzed by solid-phase ligandbinding assay. Results revealed that all R. anatipestifer isolates showed different levels of GAPDH activity except four strains, which contained a gapdh-like gene. The gapdh of R. anatipestifer, which is located phylogenetically in the same branch as enterohemorrhagic Escherichia coil （EHEC）, belonged to class I GAPDH, and encoded a 36.7-kDa protein. All RaGAPDH-encoding gene sequences from field isolates of R. anatipestiferdisplayed 100% homology. The RaGAPDH localized on the extracellular membrane of several R. anatipestifer strains. Further, it was released into the culture medium, and exhibited GAPDH enzyme activity. We also confirmed the binding of RaGAPDH to plasminogen and fibrinogen. These results demonstrated that GAPDH was present in R. anatipestifer, although not in all strains, and that RaGAPDH might contribute to the microorganism＇s virulence.

关键词： Riemerella anatipestifer Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) Extracellular protein

Kinetics and Mechanism of Oxidation of Glycol by Dihydroxydiperiodatonickelate ( Ⅳ )Complex in Aqueous Alkaline Medium

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 1992年第4期8卷 432-438页

作者： WANG An-zhou, li Feng-mai, li zhi-ting, DING tian-hui and SHAN Jin-huan (Department of Chemistry, Hebei University, Baoding, 071002)

The kinetics of oxidation of glycol by dihydroxydiperiodato nickelate ( Ⅳ) complex (DDN) was studied by spectrophotometry in aqueous alkaline medium in a temperature range of 25-40℃. The reaction was found to be first order with respect to Ni( Ⅳ ) and also to be first order with respect to glycol. The rate increases with the increase in [OH ] and decreases with the increase in [IO-4], showing that dihydroxymonoperiodatonickelate ( Ⅳ )(DMN) is the reactive species of oxidant. Salt effect indicated that the reaction is of ion-dipole type. No free radical was detected. A mechanism of an inner-sphere two-electron transfer reaction involving a preequilibrium between DDN and DMN is proposed, and the rate equation derived from the mechanism can explain all experimental observations. Activation parameters of the rate-determing step and the equilibrium constants were calculated.

关键词： Dihydroxydiperiodatonickelate (Ⅳ) Glycol Redox reaction Kinetics and mechanism

2 - 34 Solvent Extraction of Am（ Ⅲ ） and Eu（ Ⅲ ） with a Picolinamide ligand

在线全文

学校读者我要写书评

暂无评论

IMP & HIRFL Annual Report 2012年第1期 74-75页

作者： Tan Cunmin Qin zhi li Zhan tian Longlong

Clinical study of large mandibular cystic lesion treated by decompression

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中国口腔颌面外科杂志 2008年第B5期6卷 195-196页

作者： Yin Xue Min Lv Xiao zhi Zhang Lei Tao Wang li Chao tian zhi Hui Department of Stomatolosy Nanfang Hospital Southern MedicalUniversity Guant~hou 510515 China

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

第18届世界杯足球赛得分类型变化比较研究

天津体育学院学报 2007年第2期22卷 172-174页

作者：李天植沈娜延边大学体育学院延吉133002

采用实况录像法,观察统计第18届世界杯足球赛进球得分情况,并把各得分情况加以分类。分析现代顶级世界足球赛事得分趋势,探索足球比赛得分变化发展的内在规律,为足球教练员提高组织训练、指导比赛能力提供科学的依据、有效的方法、理性... 详细信息

采用实况录像法,观察统计第18届世界杯足球赛进球得分情况,并把各得分情况加以分类。分析现代顶级世界足球赛事得分趋势,探索足球比赛得分变化发展的内在规律,为足球教练员提高组织训练、指导比赛能力提供科学的依据、有效的方法、理性的指导。

关键词：足球第18届世界杯进球得分类型

在线全文

学校读者我要写书评

暂无评论

新型冠状病毒肺炎诊治研究进展

解放军医学杂志 2020年第3期45卷 260-264页

作者：李天志徐国纲解放军总医院第二医学中心国家老年疾病临床医学研究中心北京100853

新型冠状病毒肺炎(COVID-19)于2019年12月开始在我国湖北省武汉市暴发流行,部分重症患者因多脏器衰竭而死亡。新型冠状病毒(SARS-CoV-2)以呼吸道飞沫传播和密切接触传播为主,传染性强,在全球多个国家和地区出现感染和死亡病例。截至目前... 详细信息

新型冠状病毒肺炎(COVID-19)于2019年12月开始在我国湖北省武汉市暴发流行,部分重症患者因多脏器衰竭而死亡。新型冠状病毒(SARS-CoV-2)以呼吸道飞沫传播和密切接触传播为主,传染性强,在全球多个国家和地区出现感染和死亡病例。截至目前,尚无专门针对该病毒的疫苗及特效药物。该文对COVID-19的病原学特点、临床症状、感染机制、流行病学、相关药物研究进展及临床效果等进行综述,为临床防治提供参考。

关键词：新型冠状病毒肺炎 SARS-CoV-2 发病机制治疗措施

泛耐药鲍曼不动杆菌所致呼吸机相关肺炎的临床疗效分析

在线全文

学校读者我要写书评

暂无评论

中国医学科学院学报 2014年第2期36卷 185-188页

作者：王立万邹琳李洪霞李天志中国人民解放军总医院呼吸内科北京100853

目的总结泛耐药鲍曼不动杆菌(PDR-Ab)感染的临床特点,分析不同抗菌药物对PDR-Ab所致肺部感染的治疗效果,为PDR-Ab肺部感染提供临床经验与治疗方案。方法选取中国人民解放军总医院老年病区2009年4月至2010年4月老年呼吸机相关肺炎为研究... 详细信息

目的总结泛耐药鲍曼不动杆菌(PDR-Ab)感染的临床特点,分析不同抗菌药物对PDR-Ab所致肺部感染的治疗效果,为PDR-Ab肺部感染提供临床经验与治疗方案。方法选取中国人民解放军总医院老年病区2009年4月至2010年4月老年呼吸机相关肺炎为研究对象,分离获取患者下呼吸道分泌物中PDR-Ab,并对其临床分布特征、药物敏感结果以及在此基础上进行的治疗及预后等方面进行回顾性分析。结果在126例老年呼吸机相关肺炎患者中,42例以PDR-Ab为优势菌。A组20例应用头孢哌酮/舒巴坦联合米诺环素治疗,临床总有效率为65%,细菌清除率为40%。B组22例应用其他抗菌药物治疗,临床总有效率为22.7%,细菌清除率为13.6%。PDR-Ab培养阳性前住院天数和机械通气时间是影响细菌清除的主要因素(P均<0.01)。结论头孢哌酮/舒巴坦联合米诺环素对PDR-Ab引起的下呼吸道-肺感染具有一定疗效,该治疗方案在临床应用中具有重要的实际意义。

关键词：泛耐药鲍曼不动杆菌头孢哌酮舒巴坦米诺环素影响因素