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Science China(life Sciences) 2020年第12期63卷 1776-1785页

作者： Mengting li zhen-li huang Britton Chance Center for Biomedical Photonics.Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan 430074.China MoE Key Laboratory for Biomedical Photonics Collaborative Innovation Center for Biomedical Engineering.School of Engineering Sciences.Huazhong University of Science and TechnologyWuhan 430074China School of Biomedical Engineering Hainan UniversityHaikou 570228China

Resolution is undoubtedly the most important parameter in optical microscopy by providing an estimation on the maximum resolving power of a certain optical microscope. For centuries, the resolution of an optical microscope is generally considered to be limited only by the numerical aperture of the optical system and the wavelength of light. However, since the invention and popularity of various advanced fluorescence microscopy techniques, especially super-resolution fluorescence microscopy, many new methods have been proposed for estimating the resolution, leading to confusions for researchers who need to quantify the resolution of their fluorescence microscopes. In this paper, we firstly summarize the early concepts and criteria for predicting the resolution limit of an ideal optical system. Then, we discuss some important influence factors that deteriorate the resolution of a certain fluorescence microscope. Finally, we provide methods and examples on how to measure the resolution of a fluorescence microscope from captured fluorescence images. This paper aims to answer as best as possible the theoretical and practical issues regarding the resolution estimation in fluorescence microscopy.

关键词： fluorescence microscopy super-resolution microscopy Abbe limit Rayleigh criterion image resolution

INSTANTANEOUS KINEMATIC CHARACTERISTICS OF ASPECIAL 3-UPU PARALLEL MANIPULATOR

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Chinese Journal of Mechanical Engineering 2005年第3期18卷 376-381页

作者： li Shihua huang zhen College of Mechanical Engineering Yanshan University Qinhuangdao 066004 China

The instantaneous kinematics of a special 3-UPU parallel platform manipulator is discussed. First, the instantaneous motions of the 3-UPU manipulator in four kinds of positions and a special manipulator are studied by reciprocal screw theory. Then, the principal screws in one of four positions are obtained. It is shown that the moving platform has five degrees of freedom （DOF） in the initial position or after a translation along the z-axis; In the generic position, the mechanism only has three DOF, moreover the three DOF characteristics are different in different position. The instantaneous kinematic characteristics of alike 3-UPU mechanisms are very different in different position and special structure. The results presented are important to the use of alike 3-UPU parallel manipulator and contribute to the mechanism theory .

关键词： Parallel manipulator Reciprocal screw Principal screws Instantaneous kinematic characteristics

Assessing low-light cameras with photon transfer curve method

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Journal of Innovative Optical Health Sciences 2016年第3期9卷 35-51页

作者： Luchang li Mengting li Zhaoning Zhang zhen-li huang Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology Wuhan 430074 P.R.China Key Laboratory of Biomedical Photonics of Ministry of Education Department of Biomedical Engineering Huazhong University of Science and Technology Wuhan 430074P.R.China

Low-light camera is an indispensable component in various°uorescence microscopy ***,choosing an appropriate low-light camera for a speci¯c technique(for example,single molecule imaging)is always time-consuming and sometimes confusing,especially after the commercialization of a new type of camera called sCMOS camera,which is now receiving heavy demands and high praise from both academic and industrial *** this tutorial,we try to provide a guide on how to fully access the performance of low-light cameras using a well-developed method called photon transfer curve(PTC).We¯rst present a brief explanation on the key parameters for characterizing low-light cameras,then explain the experimental procedures on how to measure *** also show the application of the PTC method in experimentally quantifying the performance of two representative low-light ***,we extend the PTC method to provide o®set map,read noise map,and gain map of individual pixels inside a camera.

关键词： Photon transfer curve EMCCD sCMOS SNR

Application of super-resolution fluorescence microscopy in hematologic malignancies

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Journal of Innovative Optical Health Sciences 2022年第2期15卷 18-31页

作者： Yalan Yu Jianing Yu zhen-li huang Fuling Zhou Department of Hematology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical EngineeringHainan UniversityHaikou 570228China

Hematologic malignancies are one of the most common malignant tumors caused by the clonal proliferation and differentiation of hematopoietic and lymphoid stem *** examination of bone marrow cells combined with immunodeficiency typing is of great significance to the diagnostic type,treatment and prognosis of hematologic ***-resolution fluorescence microscopy(SRM)is a special kind of optical microscopy technology,which breaks the resolution limit and was awarded the Nobel Prize in Chemistry in *** the development of SRM,many related technologies have been applied to the diagnosis and treatment of clinical *** was reported that a major type of SRM technique,single molecule localization microscopy(SMLM),is more sensitive than flow cytometry(FC)in detecting cell membrane antigens'expression,thus enabling better chances in detecting antigens on hematopoietic cells than traditional analytic ***,SRM may be applied to clinical pathology and may guide precision medicine and personalized medicine for clone hematopoietic cell *** this paper,we mainly discuss the application of SRM in clone hematological malignancies.

关键词： Hematologic malignancies super-resolution°uorescence microscopy structured illumination microscopy stimulated emission depletion microscopy single molecule localization microscopy

Editorial-Introduction to the special issue on super-resolution optical microscopy

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Journal of Innovative Optical Health Sciences 2016年第3期9卷 1-3页

作者： zhen-li huang Ming-Qiang Zhu Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics(WNLO)Huazhong University of Science and Technology Wuhan 430074 P.R.China Wuhan National Laboratory for Optoelectronics(WNLO)Huazhong University of Science and Technology Wuhan 430074 P.R.China

Super-resolution optical microscopy,sometimes called optical nanoscopy,refers to a new kind of far-¯eld optical microscopy which allows optical imaging with a resolution higher than the well-known resolution limit(or the Abbe di®raction limit)which stood for more than a *** reported approaches that break the resolution limit involve either e®ectively reducing the focus region of the excitation light or stochastic separation and precise localization of single°***-resolution optical microscopy provides unprecedented opportunities for tackling outstanding fundamental questions in life science,medicine,materials,and many others that require non-invasive imaging with molecular level spatial resolution.

关键词： resolution limit precise

Synthesis of water-soluble cystine C_(60) derivative with catalyst and its active oxygen radical scavenging ability

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Chinese Chemical Letters 2007年第1期18卷 51-54页

作者： zhen Hu Wen Chao Guan Xiang Ying Tang li zhen huang Hong Xu Department of Chemistry Huazhong University of Science and Technology Wuhan 430074 China

A novel water-soluble cystine C60 derivative was synthesized in the presence of the catalyst, tetrabutylammonium hydroxide （TBAH）. The product was characterized by FT-IR, UV, ^1H NMR, ^13C NMR, MS and elemental analysis. Furthermore, the scavenging ability to superoxygen anion radical O2^·- and hydroxyl radical ^·OH was studied by chemiluminescence. It was found that cystine C60 derivative showed an excellent efficiency in eliminating superoxygen anion radical and hydroxyl radical. The 50% inhibition concentration （IC50） for superoxygen anion radical and hydroxyl radical were 0.167 and 0.008 mg/mL, respectively.

关键词： Cystine C60 derivatives TBAH Superoxygen anion radical Hydroxyl radical Chemiluminescence

Large Amplitude Oscillatory Shear Studies on the Strain-stiffening Behavior of Gelatin Gels

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Chinese Journal of Polymer Science 2015年第1期33卷 70-83页

作者： Wei-xiang Sun li-zhen huang Yan-rui Yang Xin-xing liu 童真 Research Institute of Materials Science and State Key Laboratory of Luminescent Materials and Devices South China University of Technology

linear and nonlinear viscoelasticity of gelatin solutions was investigated by rheology. The dynamic mechanical properties during the sol-gel transition of gelatin followed the time-cure superposition. The fractal dimension df of the critical gel was estimated as 1.76, which indicated a loose network. A high sol fraction ws = 0.61 was evaluated from the plateau modulus by semi-empirical models. Strain-stiffening behavior was observed under large amplitude oscillatory shear（LAOS） for the gelatin gel. The strain and frequency dependence of the minimum strain modulus GM, energy dissipation Ed, and nonlinear viscoelastic parameter NE was illustrated in Pipkin diagrams and explained by the strain induced helix formation reported previously by others. The BST model described the strain-stiffening behavior of gelatin gel quite well, whereas the Gent and worm-like chain network models overestimated the strain-stiffening at large strains.

关键词： Gelatin Large amplitude oscillatory shear Strain-stiffening.

DecodeSTORM:A user-friendly ImageJ plug-in for quantitative data analysis in single-molecule localization microscopy

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Journal of Innovative Optical Health Sciences 2023年第6期16卷 77-89页

作者： Qihang Song Cheng Wu Jianming huang Zhiwei Zhou zhen-li huang zhengxia Wang Key Laboratory of Biomedical Engineering of Hainan Province School of Biomedical Engineering Hainan University Haikou 570228P.R.China School of Computer Science and Technology Hainan University Haikou 570228P.R.China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China

Quantitative data analysis in single-molecule localization microscopy(SMLM)is crucial for studying cellular functions at the biomolecular *** the past decade,several quantitative methods were developed for analyzing SMLM data;however,imaging artifacts in SMLM experiments reduce the accuracy of these methods,and these methods were seldom designed as user-friendly *** are now trying to overcome these di±culties by developing easyto-use SMLM data analysis software for certain image analysis ***,this kind of software did not pay su±cient attention to the impact of imaging artifacts on the analysis accuracy,and usually contained only one type of analysis ***,users are still facing di±culties when they want to have the combined use of different types of analysis methods according to the characteristics of their data and their own *** this paper,we report an ImageJ plug-in called DecodeSTORM,which not only has a simple GUI for human–computer interaction,but also combines artifact correction with several quantitative analysis *** includes format conversion,channel registration,artifact correction(drift correction and localization¯ltering),quantitative analysis(segmentation and clustering,spatial distribution statistics and colocalization)and ***,these data analysis methods can be combined freely,thus improving the accuracy of quantitative analysis and allowing users to have an optimal combination of *** believe DecodeSTORM is a user-friendly and powerful ImageJ plug-in,which provides an easy and accurate data analysis tool for adventurous biologists who are looking for new imaging tools for studying important questions in cell biology.

关键词： Single-molecule localization microscopy artifact correction quantitative analysis ImageJ plug-in DecodeSTORM

Nano-silver induced ceramic coloring via control of glaze interface and phase separation

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Rare Metals 2021年第8期40卷 2292-2300页

作者： Cong-Xu Zhu Han-Lu Zhang Wen-Jun Fa Feng-Wei Miao Xiang-Qing Kong Chun-Sheng Kong Ming-Hua Feng Yu Wang Wei-Wei li zhen-li huang Zhi zheng Key Laboratory of Micro-Nano Materials for Energy Storage and Conversion of Henan Province College of Chemical and Materials EngineeringXuchang UniversityXuchang461000China Xuchang Hydrology and Water Resources Survey Burcau Xuchang461000China Song Dynasty Royal King Co. LtdXuchang461000China Kongjia Jun kiln Co. LtdXuchang461000China

As a kind of rare materials,nano-silver has broad application prospects in the fields of catalysis,medical treatment,new energy and so ***,there are few reports on the systematic research of nano-silver in ceramic *** this work,different color ceramic glazes were produced by tuning the nano-silver content and optimizing the firing *** crystalline phase composition,micro-morphology and elemental distribution of fired glazes were analyzed and discussed in *** elemental composition and distribution of the samples were *** surface of the glazes with varying Ag contents fired under the reducing atmosphere exhibited *** the blue color is attributable to Rayleigh scattering that may arise because the phase-separation structures existed in ***,the color of the ceramic glazes with varying Ag contents changed to golden-yellow when the ceramic glazes were fired under the air ***-yellow color of the samples fired under the air atmosphere is mainly attributable to the silver nanoparticles,though Ag^(+) may be existed in the glazes.

关键词： Ceramic glazes Nanosized silver Microscopic analysis Coloring Phase separation

Mechanical Properties of Railway High-strength Manufactured Sand Concrete: Typical lithology, Stone Powder Content and Strength Grade

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 194-203页

作者： WANG zhen li Huajian huang Fali YANG Zhiqiang WEN Jiaxin SHI Henan Railway Engineering Research Institute China Academy of Railway Science Corporation LimitedBeijing 100081China State Key Laboratory for Track Technology of High-Speed Railway Beijing 100081China

In order to achieve the large-scale application of manufactured sand in railway high-strength concrete structure,a series of high-strength manufactured sand concrete(HMC)are prepared by taking the manufactured sand lithology(tuff,limestone,basalt,granite),stone powder content(0,5%,10%,15%)and concrete strength grade(C60,C80,C100)as *** evolution of mechanical properties of HMC and the correlation between cubic compressive strength and other mechanical properties are *** to river sand,manufactured sand enhances the cubic compressive strength,axial compressive strength and elastic modulus of concrete,while its potential microcracks weaken the flexural strength and splitting tensile strength of *** powder content displays both positive and negative effects on mechanical properties of HMC,and the stone powder content is suggested to be less than 10%.The empirical formulas between cubic compressive strength and other mechanical properties are proposed.

关键词： manufactured sand concrete railway mechanical property lithology stone powder content