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Tumor-Derived Autophagosomes induce B Cell Proliferation and Activation

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Tumor-Derived Autophagosomes induce B Cell Proliferation and...

Correlation between the rock mass properties and maximum horizontal stress:A case study of overcoring stress measurements

第八届全国免疫学学术大会

作者： Weixia li Meng Zhou Hongyan Ren Meng Cao li-xin wang Department of Microbiology and Immunology Medical School of Southeast University

Introduction:Previously, we have documented that isolated autophagosomes from tumor cells after blocking protein degradation to enrich tumor specific antigens, used as therapeutic vaccines and cross-presented by dendritic cells, can efficiently cross-prime nave T cells and mediate tumor ***, the effect of tumor-derived autophagosomes, here we refer as to DRibbles, on B cells has not been studied so ***:In this study, we examined whether tumor-derived DRibbles could induce B-cell activation and proliferation and production of tumor-specific antibodies in *** addition, we investigated whether B cells could uptake and cross-present DRibbles antigens and serves as efficient antigen presenting cells for T cell ***:DRibbles were firstly isolated from Hep1-6 cells, and then intravenously injected into C57/BL6 ***1-6 tumor cell specific antibodies in serum were analyzed by flow cytometry and fluorescence microscope.B cells were purified using a combination of anti-CD43 coupled magnetic beads, and then coincubated with CFSE-labeled DRibbles in vitro.B cell proliferation was assessed by measuring the dilution of CFSE via flow cytometry.B cell activation was analyzed by measuring the activation markers on B cell surface, and supernatants were harvested for detection of cytokines and antibodies via *** DRibblesantigens presentation by B cells assay, C57/BL6 mice were intro-node vaccinated with DRibbles, CD4+T and CD8+ T cells were purified from lymph node of the vaccinated mice, and then co-incubated with purified B cells plus DRibbles for 72 hours ***-γ or IL-4 in the supernatants was tested by ***:At present study, we found that DRibbles generated from a murine hepatoma cell line Hep1-6, induced B cell activation after intravenous injection into mice.B cell populations were significantly expanded and the production of Hep1-6 tumor-specific antibodies was successfully ***, in vitro studie

关键词： tumor-derived autophagosomes DRibbles B cell activation tumor-specific antibodies antigen cross-presentation

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 39-48页

作者： Peng li Meifeng Cai Shengjun Miao Yuan li Yu wang Key Laboratory of Ministry of Education for Efficient Mining and Safety of Metal Mines University of Science and Technology BeijingBeijing 100083China State Key Laboratory of Coal Resources and Safe Mining China University of Mining and TechnologyXuzhou 221116China

Understanding the mechanical properties of the lithologies is crucial to accurately determine the horizontal stress *** investigate the correlation between the rock mass properties and maximum horizontal stress,the three-dimensional(3D)stress tensors at 89 measuring points determined using an improved overcoring technique in nine mines in China were adopted,a newly defined characteristic parameter C_(ERP)was proposed as an indicator for evaluating the structural properties of rock masses,and a fuzzy relation matrix was established using the information distribution *** results indicate that both the vertical stress and horizontal stress exhibit a good linear growth relationship with *** is no remarkable correlation between the elastic modulus,Poisson's ratio and depth,and the distribution of data points is scattered and ***,there is no obvious relationship between the rock quality designation(RQD)and *** maximum horizontal stress σ_(H) is a function of rock properties,showing a certain linear relationship with the C_(ERP)at the same *** addition,the overall change trend of σ_(H) determined by the established fuzzy identification method is to increase with the increase of C_(ERP).The fuzzy identification method also demonstrates a relatively detailed local relationship betweenσ_H and C_(ERP),and the predicted curve rises in a fluctuating way,which is in accord well with the measured stress data.

关键词： overcoring stress measurements elastic modulus Poisson's ratio rock quality designation maximum horizontal stress fuzzy identification

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汽轮机轴封系统的通用计算模型及其应用

中国电机工程学报 2008年第20期28卷 27-31页

作者：张春发李新旺李娟华北电力大学能源与动力工程学院河北省保定市071003

热力系统中,汽轮机轴封系统对机组运行的安全和经济性具有十分重要的意义。以矩阵为数学基础,推导出了轴封系统的通用计算模型。在利用通用模型进行分析时,针对汽轮机轴封系统本身的特点,将轴封渗漏及其利用系统划分成轴封供汽和轴封回... 详细信息

热力系统中,汽轮机轴封系统对机组运行的安全和经济性具有十分重要的意义。以矩阵为数学基础,推导出了轴封系统的通用计算模型。在利用通用模型进行分析时,针对汽轮机轴封系统本身的特点,将轴封渗漏及其利用系统划分成轴封供汽和轴封回汽系统两部分,从而将辅助汽流按部位进行了归类。该方法采用矩阵形式表示,计算模型与热力系统结构之间一一对应。应用表明,该种分析思路更加清晰,并具有计算简洁、通用性强等优点,尤其适用于计算机编程。

关键词：汽轮机轴封矩阵通用计算模型热力系统热经济性

Roles of cyclooxygenase-2 in microvascular endothelial cell proliferation induced by basic fibroblast growth factor

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Chinese Medical Journal 2008年第24期121卷 2599-2603页

作者： QIAN Rui-zhe YUE Fei ZHANG Guo-ping HOU li-kun wang xin-hong JIN Hui-ming Department of Physiology and Pathophysiology Shanghai Medical College Fudan University Shanghai 200032 China

Background The level of basic fibroblast growth factor （bFGF） increases rapidly after cerebral ischemia. However, the molecular mechanisms for the effects of bFGF on cerebral microvascular endothelial cells （cMVECs） have not yet been fully elucidated. In this study, a murine cMVEC line, bEnd.3, was employed to study the effects of bFGF on cyclooxygenase （COX） expression and its downstream effects in cMVECs. Methods After treatment with bFGF, RT-PCR and Western blotting analyses were carried out to evaluate the changes in COX-2 mRNA and protein expression, respectively. MTT assays were performed to measure cell proliferation. The prostaglandin E2 （PGE2） and vascular endothelial growth factor （VEGF） concentrations in the culture medium were measured by enzyme-linked immunosorbent assay （EliSA）. Results COX-2 mRNA and protein expressions in bEnd.3 cells were induced by bFGF in time- and dose-dependent manners. The bFGF-induced COX-2 upregulation led to enhanced PGE2 production by bEnd.3 cells, and this effect was abolished by the selective COX-2 inhibitor NS-398. bFGF also increased VEGF production by bEnd.3 cells, and this effect was blocked by NS-398 and the EP1/2 （PGE2 receptors） antagonist AH6809. Furthermore, exogenous PGE2 increased VEGF production in bend.3 cells, and AH6809 blocked this effect. Conclusion bFGF increases VEGF production in an autocrine manner by increasing COX-2-generated PGE2 in cMVECs and subsequently stimulates MVEC proliferation and angiogenesis.

关键词： cerebral ischemia cerebral microvascular endothelial cell basic fibroblast growth factor cyclooxygenase prostaglandin E2 vascular endothelial growth factor

相对论性Birkhoff系统的lie对称性和守恒量

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物理学报 2000年第6期49卷 1023-1027页

作者：傅景礼王新民商丘师范学院物理系商丘476000

给出相对论性Birkhoff系统的Pfaff Birkhoff原理和Birkhoff方程 .由微分方程在无限小变换下的不变性 ,定义相对论性Birkhoff系统无限小变换生成元 ,建立lie对称的确定方程 ,得到结构方程和守恒量 .并研究该系统的lie对称性逆问题 .

关键词：相对论性Birkhoff系统 lie对称性守恒量

Cyano migration-mediated radical-polar crossover cyclopropanation

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Science China Chemistry 2025年第1期68卷 241-248页

作者： Ziqiang wang Yasu Chen Jie li Chen Zhu Key Laboratory of Organic Synthesis of Jiangsu Province College of Chemistry Chemical Engineering and Materials ScienceSoochow University Frontiers Science Center for Transformative Molecules Zhangjiang Institute for Advanced Study and Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs Shanghai Jiao Tong University

We report a novel cyano migration-mediated radical-polar crossover process, whereby both the sulfonylcyanation of aliphatic alkenes and the formation of cyclopropanes are concurrently achieved. This transformation yields a diverse array of multisubstituted cyclopropanes under mild photocatalytic conditions. Mechanistic studies elucidate that the reaction proceeds via intramolecular nucleophilic substitution, leading to the formation of the cyclopropane derivatives.

关键词： cyclopropanation cyano migration radical-polar crossover radical reactions

Design of a cationic accelerator enabling ultrafast ion diffusion kinetics in aqueous zinc-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 377-384页

作者： Yawei Xiao Qianqian Gu Haoyu li Mengyao li Yude wang National Center for International Research on Photoelectric and Energy Materials School of Materials and Energy Yunnan University Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources School of Chemistry and Chemical Engineering Hainan University College of Chemical Engineering Fuzhou University Yunnan Key Laboratory of Carbon Neutrality and Green Low-carbon Technologies Yunnan University

Aqueous zinc-ion batteries are highly favored for grid-level energy storage owing to their low cost and high safety,but their practical application is limited by slow ion *** address this,a strategy has been developed to create a cation-accelerating electric field on the surface of the cathode to achieve ultrafast Zn2+diffusion *** employing electrodeposition to coat MoS2on the surface of BaV6O16·3H2O nanowires,the directional built-in electric field generated at the heterointerface acts as a cation accelerator,continuously accelerating Zn2+diffusion into the active *** optimized Zn2+diffusion coefficient in CC@BaV-6O16·3H2O@MoS2(7.5×10～8cm2s-1) surpasses that of most reported V-based ***,MoS2serving as a cathodic armor extends the cycling life of the Zn-CC@BaV6O16·3H2O@MoS2full batteries to over 10000 *** work provides valuable insights into optimizing ion diffusion kinetics for high-performance energy storage devices.

关键词： Zinc alloys

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装载机数字液压传动系统换挡策略

吉林大学学报（工学版） 2017年第3期47卷 819-826页

作者：王丽刘昕晖王昕陈晋市梁燚杰吉林大学机械科学与工程学院长春130022 吉林大学汽车仿真与控制国家重点实验室长春130022 吉林大学珠海学院机械与汽车工程系广东珠海519041

针对现有装载机液力传动效率低、液压传动成本高等不足,基于数字液压元件及其控制技术,提出了数字控制静液传动有级自动变速方案。以发动机稳定运转在经济区域内为目标,结合装载机铲掘工况,设计了数字泵和数字马达排量的模糊换挡控制策... 详细信息

针对现有装载机液力传动效率低、液压传动成本高等不足,基于数字液压元件及其控制技术,提出了数字控制静液传动有级自动变速方案。以发动机稳定运转在经济区域内为目标,结合装载机铲掘工况,设计了数字泵和数字马达排量的模糊换挡控制策略,实现了数字泵与发动机、数字马达与变化负载的动态匹配。通过AMESim仿真验证了该方案的可行性,本研究为装载机传动系统设计提供了一种新思路,对其他行走工程机械传动系统设计具有一定参考意义。

关键词：流体传动与控制数字液压静液传动有级自动变速模糊控制

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中枢胆碱能系统对吗啡成瘾的影响研究现状

心理科学进展 2008年第3期16卷 418-423页

作者：李新旺于萍首都师范大学心理系北京100089

介绍了中枢胆碱能系统对吗啡成瘾的影响及其机制。研究结果表明,吗啡成瘾过程中伏隔核等脑区细胞间乙酰胆碱水平发生了改变;去除伏隔核等脑区胆碱能细胞强化了吗啡成瘾行为;胆碱能激动剂和抑制剂都能干预吗啡成瘾、但机制可能不同——... 详细信息

介绍了中枢胆碱能系统对吗啡成瘾的影响及其机制。研究结果表明,吗啡成瘾过程中伏隔核等脑区细胞间乙酰胆碱水平发生了改变;去除伏隔核等脑区胆碱能细胞强化了吗啡成瘾行为;胆碱能激动剂和抑制剂都能干预吗啡成瘾、但机制可能不同——前者可能通过与多巴胺能交互作用来实现,后者可能通过干扰记忆或者加速吗啡代谢而发挥作用;胆碱能受体对吗啡成瘾也具有重要影响。

关键词：吗啡成瘾乙酰胆碱胆碱能激动剂胆碱能抑制剂