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Impacts of channel morphology on residues and ecological risks of polychlorinated biphenyls in water and sediment in Chahe River

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Water Science and Engineering 2016年第4期9卷 300-311页

作者： Zhen-hua Zhao Jie Sun xiao-kun Fang li-ling xia Javid Hussain Department of Plant Soil and Microbial Sciences Michigan State University East Lansing MI 48824 USA Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes of Ministry of Education Hohai University Nanjing 210098 China College of Environment Hohai University Nanjing 210098 China Nanjing Institute of Industry Technology Nanjing 210016 China

The impacts of channel morphology on the residues and ecological risks of 14 polychlorinated biphenyl(PCB) congeners in water and sediment were investigated in summer(July) and autumn(September) in the Chahe River,in Nanjing,*** residual concentrations of trichlorobiphenyls(tri-CBs,PCB 18) and tetra-CBs(PCB 52) in water were significantly higher than those of penta-CBs to deca-CBs,and the average residual concentration of ∑PCBs(sum of 14 PCB congeners) in summer was about six times higher than in ***,the residues in sediment did not change *** analysis(RDA) indicated that channel morphology and the corresponding environmental indices had significant impacts on PCB residues and their composition profiles in water and *** overflow weir and lake-type watercourse may remarkably reduce the residual concentration and ecological risks of PCBs in *** highest reduction percentages of the residual concentration and ecological risks of ∑PCBs induced by an overflow weir were 78%and 67%,respectively,and those induced by a lake-type watercourse were 36%and 70%,*** watercourses with different channel morphologies were ranked by residual ∑PCBs concentrations in the following descending order:the natural ecological watercourse,vertical concrete watercourse,and vegetation-type riprap ***,they were ranked by residual ∑PCBs concentrations in sediment in the following descending order:the vertical concrete watercourse,vegetation-type riprap watercourse,and natural ecological watercourse.

关键词： Channel morphology RDA Ecological risk Toxic equivalent concentration

Cloning and characterization of a novel hepatitis B virus core binding protein C12

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World Journal of Gastroenterology 2005年第36期11卷 5666-5671页

作者： Yin-Ying Lu Jun Cheng Yong-Ping Yang Yan liu lin Wang Ke li ling-xia Zhang Gene Therapy Research Center Institute of Infectious Diseases The 302 Hospital of PLA 100 Xisihuanzhong Road Beijing 100039 China

AIM： To elucidate the biological function of HBV core antigen （HBcAg） on pathogenesis of hepatitis B, a novel gene C12 coding for protein with unknown function interacting with HBcAg in hepatocytes was identified and characterized. METHODS： HBcAg bait plasmid pGBKT7-HBcAg was constructed and transformed into yeast AH109, then the transformed yeast was mated with yeast Y187 containing liver complementary DNA （cDNA） library plasmid in 2xYPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium （SD/-Trp-Leu-His-Ade） and synthetic dropout nutrient medium （SD/-Trp-Leu-His-Ade） containing X-α-gal for screening twice. After extracting and sequencing of plasmid from blue colonies, we isolated a cDNA clone encoding a novel protein designated as C12 that directly interacted with HBcAg. The interaction between HBcAg and C12 was verified again by re-mating. pEGFP-N1-C12 fluorescent protein fusion gene was transfected in 293 and L02 cell, and observed by fluorescent microscope. M-FI reduction assay was used to study the action of C12 protein effect on metabolism of mammal cell. Yeast two-hybrid and cDNA microarray were performed to search binding protein and differential expression genes regulated by C12 protein. RESULTS： C12 gene was screened and identified by yeast two-hybrid system 3. The interaction between HBcAg and the novel protein coded by the new gene C12 was further confirmed by re-mating. After 48 h, fluorescence of fusion protein could be observed steadily in the 293 and L02 cell plasma. Under MTT assay, we found that the expression of C12 did not influence the growth of liver cells. Seventeen differential expression genes in HepG2 cells transfected with C12 protein expression plasmid by cDNA microarray, of which 16 genes were upregulated and 1 gene was downregulated by C12 protein. Twenty-one colonies containing 16 different genes coding for C12 protein binding proteins were isolated by yeast two-hybrid, there were 2 new genes with unknown function.

关键词： Hepatitis B virus core antigen Yeast two-hybrid cDNA microarray MTT assay

Preparation of human single chain Fv antibody against hepatitis C virus E2 protein and its identification in immunohistochemistry

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World Journal of Gastroenterology 2002年第5期8卷 863-867页

作者： Yan-Wei Zhong Jun Cheng Gang Wang Shuang-Shuang Shi li li ling-xia Zhang Ju-Mei Chen Gene Therapy Research Center,Institute of Infectious Diseases,302 Hospital of PLA,26 Fengtai Road,Beijing 100039,China Gene Therapy Research Center Institute of Infectious Diseases 302 Hospital of PLA 26 Fengtai Road Beijing 100039 China. zyw@***

AIM: To screen human single chain Fv antibody (scFv)against hepatitis C virus E2 antigen and identify its applicationin ***: The phage antibody library was panned by HCVE2 antigen, which was coated in microtiter plate. After fiverounds of biopanning,56 phage clones were identified specificto HCV E2 antigen. The selected scFv clones were digestedby SfiI/NotI and DNA was sequenced. Then it was subclonedinto the vector pCANTABSE for expression as E-taggedsoluble scFv. The liver tissue sections from normal personand patients with chronic hepatitis B and chronic hepatitis Cwere immunostained with HCV E2 scFv ***: The data of scFv-E2 DNA digestion and DNAsequencing showed that the scFv gene is composed of 750bp. EliSA and immunohistochemistry demonstrated that thehuman single chain Fy antibody against hepatitis C E2 antigenhas a specific binding character with hepatitis virus E2 antigenand paraffin-embedded tissue, but did not react with liver tissuesfrom healthy persons or patients with chronic hepatitis ***: We have successfully screened andidentified HCV E2 scFv and the scFv could be used in theimmunostaining of liver tissue sections from patients withchronic hepatitis C.

关键词：丙型肝炎病毒E2 人体单克隆抗体Fv 免疫组织化学鉴定慢性丙型肝炎

Sr_3Al_2O_6∶Eu^(2+)红色发光纳米晶的新型形貌与发光性能的研究

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无机化学学报 2007年第9期23卷 1679-1682页

作者：张平李玲霞徐明霞天津大学电子信息工程学院天津300072 天津大学材料科学与工程学院天津300072

对于稀土发光纳米晶来讲,产品颗粒尺寸与形貌不仅对发光性能有影响,而且直接影响其应用。如颗粒尺寸在1-10μm之间且形貌接近于球形的发光微米晶在涂屏时发光效率是最高的．而具有新型微观结构与形貌的稀土发光纳米晶，如纳米线、纳米... 详细信息

对于稀土发光纳米晶来讲,产品颗粒尺寸与形貌不仅对发光性能有影响,而且直接影响其应用。如颗粒尺寸在1-10μm之间且形貌接近于球形的发光微米晶在涂屏时发光效率是最高的．而具有新型微观结构与形貌的稀土发光纳米晶，如纳米线、纳米棒及具有自组装特性的花晶等纳米结构材料由于在纳米发光器件方面有着潜在的应用价值．已成为当今纳米科学的研究焦点。近年来．人们采用各种软化学方法合成发光纳米晶．工作主要集中于纳米材料合成方法的应用、比较新型纳米发光材料与常规发光材料的优缺点、颗粒大小对发光性能的影响等，

关键词： Sr3Al2O6 花状形貌纳米晶体微波干燥溶胶-凝胶法

花状Sr3Al2O6：Eu^2＋晶体的干燥方式与发光性能

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天津大学学报 2008年第1期41卷 44-47页

作者：张平李玲霞徐明霞天津大学电子信息工程学院天津300072 天津大学材料科学与工程学院天津300072

为研究稀土发光纳米晶的微观结构和形貌对发光性能的影响,用凝胶-微波干燥法和凝胶-烘箱干燥法制备了花状形貌的Sr3Al2O6晶体.研究了微波干燥和烘箱干燥两种不同的干燥方式对溶胶-凝胶法制备的Sr3Al2O6∶Eu2+发光粉体粒径、形貌、团聚... 详细信息

为研究稀土发光纳米晶的微观结构和形貌对发光性能的影响,用凝胶-微波干燥法和凝胶-烘箱干燥法制备了花状形貌的Sr3Al2O6晶体.研究了微波干燥和烘箱干燥两种不同的干燥方式对溶胶-凝胶法制备的Sr3Al2O6∶Eu2+发光粉体粒径、形貌、团聚程度、干燥时间及发光性能等的影响,结果表明采用微波干燥不仅可以大大缩短干燥所需时间,而且有利于减弱Sr3Al2O6∶Eu2+发光粉料的团聚和团聚程度,制得了颗粒分散均匀、团聚程度低、发光强度高的Sr3Al2O6∶Eu2+花状形貌粉体.研究了Sr3Al2O6∶Eu2+发光粉体的新型形貌对发光性能的影响,微波干燥法制备的均匀分散的花状形貌发光粉体发光强度高,余辉时间长达20 min.

关键词： Sr3Al2O6:Eu^2+ 花状晶体微波干燥溶胶-凝胶法

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清代台湾原住民不同社群的地权争夺

厦门大学学报（哲学社会科学版） 2010年第6期60卷 90-95页

作者：李凌霞厦门大学人类学与民族学系福建厦门361005

清代台湾在汉人移垦的推动下,汉"番"土地矛盾日渐突出,清政府基于治安考虑,划界隔离汉"番",并于乾隆年间派"熟番"于番界险要处设隘把守。随着土地资源竞争日趋白热化,"熟番"不同社群以守界名义争夺界外荒埔的地权,这个过程推动了清政府... 详细信息

清代台湾在汉人移垦的推动下,汉"番"土地矛盾日渐突出,清政府基于治安考虑,划界隔离汉"番",并于乾隆年间派"熟番"于番界险要处设隘把守。随着土地资源竞争日趋白热化,"熟番"不同社群以守界名义争夺界外荒埔的地权,这个过程推动了清政府汉"番"隔离政策的变化,促进了国家行政力量在原住民社会的深化,体现出中华民族多元一体格局在台湾实现的由经济而政治的历史逻辑。这对中华民族多元一体格局的形成是个很好的地方性注释。

关键词：清代台湾原住民 “熟番” 地权

华夷秩序中的“他者”再现:清代台湾番俗图研究

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贵州民族研究 2015年第7期36卷 167-170页

作者：李凌霞吉首大学历史与文化学院湖南吉首416000

清代台湾番俗图以图文并茂的形式呈现了台湾少数民族的形象、风俗、生计,其视觉表现形式与传统帝国华夷观、清代台湾边疆的社会历史状况相互呼应。作为对台湾边疆"他者"想象与再现的艺术形式,番俗图不只隐含着中央朝廷控制和管理台湾边... 详细信息

清代台湾番俗图以图文并茂的形式呈现了台湾少数民族的形象、风俗、生计,其视觉表现形式与传统帝国华夷观、清代台湾边疆的社会历史状况相互呼应。作为对台湾边疆"他者"想象与再现的艺术形式,番俗图不只隐含着中央朝廷控制和管理台湾边疆的政策信息,也隐约揭示了汉番融合发展的历史痕迹。

关键词：华夷秩序番俗图清代台湾 “他者”

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胡适的科学文化观

自然辩证法研究 2008年第4期24卷 101-104页

作者：李霞玲中国地质大学政法学院武汉430074

胡适作为上个世纪初中国思想史和科学史上最有影响的代表人物之一,在他践行科学中形成了一套系统的科学文化观:文化必须建立在科学基础之上,并能促进人类的福利事业,中西文化要发展,必须科学化;科学的目的是致知和求真,而实证性、合理... 详细信息

胡适作为上个世纪初中国思想史和科学史上最有影响的代表人物之一,在他践行科学中形成了一套系统的科学文化观:文化必须建立在科学基础之上,并能促进人类的福利事业,中西文化要发展,必须科学化;科学的目的是致知和求真,而实证性、合理性、普遍性则是科学的特性;他提倡的科学方法主要是两个方面:一是实验主义,二是假设主义;胡适提倡的科学精神中,首推怀疑精神,也包括创新精神和求实精神。这些精神的倡导对于弘扬科学精神,扫除愚昧迷信,输入学理,再造文明都有积极的意义。

关键词：胡适科学文化

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高氮不锈钢固溶时效碳氮化物析出相分析

冶金分析 2011年第9期31卷 1-6页

作者：李玲霞李南荣凡钢铁研究总院

采用物理化学相分析方法研究了高氮奥氏体不锈钢固溶时效后的碳、氮化物析出行为;通过实验确定了高氮钢中析出相的电化学萃取方法,钢中析出相的类型、粒度分布、含量及组成结构式;对于化学性质相似的M23C6和(CrFe)2N1-x,借助于X射线衍... 详细信息

采用物理化学相分析方法研究了高氮奥氏体不锈钢固溶时效后的碳、氮化物析出行为;通过实验确定了高氮钢中析出相的电化学萃取方法,钢中析出相的类型、粒度分布、含量及组成结构式;对于化学性质相似的M23C6和(CrFe)2N1-x,借助于X射线衍射定量的方法测定了M23C6和(CrFe)2N1-x的分量。研究结果表明,氮含量低的0#钢以M23C6型碳化物析出为主,随时效时间的延长,伴随M23C6的析出还有少量(CrFe)2N1-x氮化物析出。氮含量高的1#和2#钢以(CrFe)2N1-x型氮化物析出为主。高氮奥氏体不锈钢中析出物的总量随时效时间的延长而增加。

关键词：高氮奥氏体不锈钢固溶时效物理化学相分析