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中风醒脑液通过抑制神经元铁死亡发挥抗脑出血损伤的作用机制研究

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中国中药杂志 2024年第17期49卷 4723-4733页

作者：李孟英郭建文邵翀羽泮智勇陈天杭周科峰周惠芬万海同浙江中医药大学第一附属医院浙江杭州310006 浙江中医药大学生命科学学院浙江杭州310053 浙江中医药大学基础医学院浙江杭州310053 全省中医心脑血管病重点实验室浙江杭州310053 广州中医药大学第二附属医院省部共建中医湿证国家重点实验室广东广州510120 广州中医药大学第二附属医院脑病中心广东广州510120

中风醒脑液在临床上已被用于治疗脑出血。然而,目前对中风醒脑液的药理作用尚不明确。探讨中风醒脑液的药理作用对指导脑血管疾病的治疗至关重要。向大鼠右侧尾状核注射Ⅶ型胶原酶建立体内脑出血模型。将SD大鼠随机分成5组,用Bederson... 详细信息

中风醒脑液在临床上已被用于治疗脑出血。然而,目前对中风醒脑液的药理作用尚不明确。探讨中风醒脑液的药理作用对指导脑血管疾病的治疗至关重要。向大鼠右侧尾状核注射Ⅶ型胶原酶建立体内脑出血模型。将SD大鼠随机分成5组,用Bederson评分法评估大鼠术后的神经功能缺损情况。核磁共振成像(MRI)用于评估脑出血的体积。苏木精-伊红(HE)染色观察脑组织病理改变,透射电镜(TEM)观察脑组织超微结构变化。流式细胞术测定活性氧(ROS)水平。免疫荧光双染法检测脑组织血肿周围神经元中铁转运蛋白标记物谷胱甘肽过氧化物酶4(GPX4)的表达。Western blot法检测脑出血后RNA解旋酶和ATP酶1(UPF1)、铁泵蛋白(FPN)、酰基辅酶A连接酶4(ACSL4)、环氧化酶2(COX2)、GPX4、NADPH氧化酶1(NOX1)和胱氨酸/谷氨酸逆向转运蛋白(SLC7A11)的表达。与模型组相比,中风醒脑液治疗5 d后大鼠神经功能障碍明显减轻,血肿体积减小,脑出血病理损伤减轻;与此同时,Fe^(2+)和氧化应激水平显著降低,抑制铁死亡的蛋白表达量显著增加。综上,中风醒脑液可通过显著抑制神经元铁死亡改善大鼠脑出血的预后,为中风醒脑液的临床应用提供了有价值的指导。中风醒脑液的抗铁死亡作用可能与促进膜转运蛋白SLC7A11和FPN的表达有关。

关键词：脑出血铁死亡中风醒脑液神经元死亡

致脑膜炎猪链球菌2型的分子分型鉴定及其生物学特性

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畜牧兽医学报 2024年第3期55卷 1192-1207页

作者：李芃绪李世景孙骏项维赵苗苗侯天牧李华明广敏陈瑞格徐梦然吴晓敏姜合祥雷连成张付贤长江大学动物科学技术学院荆州434023 吉林大学动物医学学院长春130062

旨在了解猪源致脑膜炎猪链球菌2型的分子分型和致病性及其生物学特性。本研究从患病猪的脑组织中分离到1株猪链球菌PX0923,通过电镜观察、16S rRNA基因测序、多重PCR、PCR-RFLP和多位点序列分析(MLST)等方法对其进行血清型和分子分型鉴... 详细信息

旨在了解猪源致脑膜炎猪链球菌2型的分子分型和致病性及其生物学特性。本研究从患病猪的脑组织中分离到1株猪链球菌PX0923,通过电镜观察、16S rRNA基因测序、多重PCR、PCR-RFLP和多位点序列分析(MLST)等方法对其进行血清型和分子分型鉴定,毒力基因、生物被膜形成能力、耐药基因检测、小鼠感染试验、抗生素和中药药敏试验分析分离株PX0923的致病性和药物敏感性。结果表明,分离菌株PX0923为革兰阳性菌,电镜下可见连续排列成链状的卵圆形菌体;PCR和16S rRNA基因系统发育树分析确定其属于猪链球菌2型;序列分型为ST7,与ST1型猪链球菌聚为一支;携带epf+/mrp+/sly+/gapdh+/fbps+/orf2+/sao+7种毒力基因,呈现α型溶血活性;感染小鼠可导致小鼠肺、脾和肝等多个器官不同程度的病变,出现了小鼠脑膜炎等与病猪类似的临床症状;半数致死量(LD50)为1.08×10^(9)CFU/小鼠,提示PX0923可引起小鼠脑膜炎。分离菌株对庆大霉素、卡那霉素、新霉素、阿米卡星等12种药物耐药,携带耐药基因:aadA1和bla-TEM,对中药全蝎和三七敏感。本研究分离到猪源猪链球菌PX0923为血清2型,MLST分型为ST7,其携带多种毒力基因和耐药基因,感染小鼠可引起脑膜炎等多组织损伤;对多种抗生素和中药全蝎、三七敏感。研究结果为猪链球菌的流行和溯源分析提供了分子生物学基础,对致脑膜炎猪链球菌感染的防控提供参考依据。

关键词：猪链球菌2型分子分型 ST7型生物学特性耐药性中药

积极心理学联合改良细节管理对ICU清醒患者心理压力、特质焦虑和ICU综合征发生率的影响

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中国健康心理学杂志 2024年第8期32卷 1162-1168页

作者：李文霞侯守超邹辉煌邢焕民河南省人民医院ICU中心一病区郑州450000

目的:探究积极心理学联合改良细节管理对重症监护病房(ICU)清醒患者心理压力、特质焦虑和ICU综合征发生率的影响。方法:将某院2020年1月到2021年9月实施常规干预的ICU清醒患者61例作为对照组,将2021年10月到2023年6月在常规干预基础上... 详细信息

目的:探究积极心理学联合改良细节管理对重症监护病房(ICU)清醒患者心理压力、特质焦虑和ICU综合征发生率的影响。方法:将某院2020年1月到2021年9月实施常规干预的ICU清醒患者61例作为对照组,将2021年10月到2023年6月在常规干预基础上实施积极心理学联合改良细节管理的ICU清醒患者67例作为观察组。比较两组干预前后心理压力[中文版压力知觉量表(CPSS)]、焦虑状况[状态-特质焦虑量表(STAI)]、自我效能感[一般自我效能感量表(GSES)]、睡眠质量[匹兹堡睡眠质量指数(PSQI)]及ICU综合征发生情况。结果:观察组干预后CPSS各维度及总评分均低于对照组(t=3.458,2.805,3.870;P<0.05);观察组干预后STAI特质焦虑维度评分及总评分均低于对照组(t=2.899,2.604;P<0.05);观察组GSES评分高于对照组(t=2.433,P<0.05);观察组PSQI各维度评分均低于对照组(t=2.682,2.675,2.468,2.329,3.156,2.722,2.586;P<0.05);干预期间,观察组患者ICU综合征发生率低于对照组(χ^(2)=5.155,P<0.05),观察组患者ICU综合征症状持续时间短于对照组(t=2.453,P<0.05)。结论:积极心理学联合改良细节管理对ICU清醒患者的干预效果显著,能减轻患者心理压力及特质焦虑,提升其自我效能感,改善睡眠质量,降低ICU综合征发生率。

关键词：积极心理学改良细节管理 ICU清醒患者心理压力特质焦虑 ICU综合征

花岗岩成矿元素迁移富集的分析方法——以滇西南大同山稀土矿区矿化与风化过程为例

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地质与资源 2024年第3期33卷 323-330页

作者：顾光明李杭严城民马重辉雷星胡凯兵云南省核工业地质调查院云南昆明650106 云南省地质矿产勘查开发局区域地质矿产调查大队云南玉溪653100

研究区位于滇西南临沧花岗岩中南段.为查证稀土矿体中有色金属、稀有金属的共伴生情况,在钻孔的稀土矿体中采集30件化学分析样品,分析元素为W、Sn、Nb、Ta、Rb、Cs.对花岗岩成矿元素迁移富集的研究,采用了如下方法步骤:1)将各元素的分... 详细信息

研究区位于滇西南临沧花岗岩中南段.为查证稀土矿体中有色金属、稀有金属的共伴生情况,在钻孔的稀土矿体中采集30件化学分析样品,分析元素为W、Sn、Nb、Ta、Rb、Cs.对花岗岩成矿元素迁移富集的研究,采用了如下方法步骤:1)将各元素的分析值由大到小顺序排列并划分为6个组,每组5个数据;进行观察后发现21件样品未发生矿化,4件样品发生过Sn矿化,4件样品发生过Rb矿化,1件样品发生过Sn、Rb矿化.2)在未发生矿化的样品中,将风化壳与未风化岩石的元素质量分数进行对比,定量评估岩石在风化过程中元素的迁移富集程度;与该地区基岩相比较,风化壳中W、Sn、Nb、Ta质量分数增高(元素富集),Rb、Cs质量分数下降(元素迁移).3)将Sn、Rb矿化样品的平均值与非矿化样品的平均值进行对比,定量评估岩石的矿化程度;发生矿化的样品平均值中,Sn矿化伴有较弱的W、Ta矿化,Rb矿化伴有明显的W、Ta、Cs矿化,Sn、Rb矿化伴生明显的W、Ta、Cs矿化.

关键词：花岗岩成矿元素稀土矿迁移富集分析方法云南省

Image processing based three-dimensional model reconstruction for cross-platform numerical simulation

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China Foundry 2023年第2期20卷 139-147页

作者： Yu-cheng Sun Yu-hang Huang Na li Xiao Han Ai-long Jiang Jin-wu Kang Ji-wu Wang Hai-liang Yu State Key Laboratory of Engine Reliability Weichai Power Co.Ltd.Weifang 261061ShandongChina School of Mechanical Engineering Central South UniversityChangsha 41000China School of Mechanical Electronic and Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Key Laboratory for Advanced Materials Processing Technology School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

Numerical simulation is the most powerful computational and analysis tool for a large variety of engineering and physical *** a complex problem relating to multi-field,multi-process and multi-scale,different computing tools have to be developed so as to solve particular fields at different scales and for different ***,the integration of different types of software is ***,it is difficult to perform the transfer of the meshes and simulated results among software packages because of the lack of shared data formats or encrypted data *** image processing based method for three-dimensional model reconstruction for numerical simulation was proposed,which presents a solution to the integration problem by a series of slice or projection images obtained by the post-processing modules of the numerical simulation *** means of mapping image pixels to meshes of either finite difference or finite element models,the geometry contour can be extracted to export the stereolithography *** values of results,represented by color,can be deduced and assigned to the *** the models with data can be directly or indirectly integrated into other software as a continued or new numerical *** three-dimensional reconstruction method has been validated in numerical simulation of castings and case studies were provided in this study.

关键词： cross-platform numerical simulation 3D model reconstruction image processing slice

Study on a noninvasive method for rapid screening Human Serum albumin injectables by Raman spectroscopy

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Journal of Innovative Optical Health Sciences 2017年第1期10卷 34-42页

作者： Yu Zhao Nan Ji Xiuji li Jifeng hou Min li Yongjian Yang Ping Tian Xinmei Sun Xinyue Xiao lihui Yin National Institutes of Food and Drug Control(NIFDC)No.2 Tiantan Xi li BeijingP.R.China tSichuan Vspec Technologies Co.Ltd.Beijing Office No.2 BeiTaiPingZhuang RoadBeijingP.R.China Shanghai Institute for Food and Drug Control(SIFDC)No.1500 Zhangheng Road ShanghaiP.R.China

Human serum albumin(HSA)injectable product is a severely afflicted area on drug safety due to its high price and restricted *** spectroscopy performances high specificity on HSA detection and it is even possible to determine HSA injectable products *** this study,we developed a noninvasive rapid screening method for of HSA injectable products by using portable Raman *** models were established by using principal component analysis combined with classical least squares(PCA-CLS)algorithm,while quanti-tative model was established by using partial least squares(PLS)*** transfer in different instruments of both the same and different apparatus modules was further discussed in this paper.A total of 34 HSA injectable samples collected from markets were used for *** identification results showed 100%accuracy and the predicted concentrations of those identified as true HSA were consistent with their labeled *** quantitative results also indicated that model transfer was excellent in the same apparatus modules of Raman spectrometer at all concentration levels,and still good enough in the different apparatus modules although the relative standard deviation(RSD)value showed a little increasing trend at low HSA concentration *** conclusion,the method was proved to be feasible and efficient for screening HSA injections,especially on its screening speed and the consideration of glass ***,with inspiring results on the model transfer,the method could be used as a universal screening mean to different Raman instruments.

关键词： Human serum albumin Raman noninvasive rapid screening method principal component analysis combined with classical least squares partial least squares model transfer.

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相机与激光雷达融合的道路目标检测研究

广西科技大学学报 2024年第1期35卷 84-93页

作者：赵浙栋张成涛李习刊杨航覃立仁广西科技大学机械与汽车工程学院广西柳州545616

目标检测是自动驾驶感知系统的基础。由于单一传感器的感知存在时空盲区问题,本文提出一种基于相机与激光雷达的后融合目标检测算法。该算法采用改进后的YOLOv5s视觉目标检测算法来检测目标类别,其平均精度均值提高了2.75%。激光雷达感... 详细信息

目标检测是自动驾驶感知系统的基础。由于单一传感器的感知存在时空盲区问题,本文提出一种基于相机与激光雷达的后融合目标检测算法。该算法采用改进后的YOLOv5s视觉目标检测算法来检测目标类别,其平均精度均值提高了2.75%。激光雷达感知采用分段聚类半径的欧几里得聚类算法对预处理后的点云信息进行聚类,并检测出检测区域内物体的目标距离。通过标定的参数,将点云投影到图像上,以融合感知结果来确定检测对象的类别和距离。本文在相关工况环境下对算法进行了验证和测试,结果表明:本文提出的目标检测算法的检出率为88.9%,比单一相机感知的检出率提高了7.8%。

关键词： YOLOv5s 激光雷达多传感器融合目标检测

Mechanical and corrosion properties of Al/Ti film on magnesium alloy AZ31B

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Frontiers of Materials Science 2015年第1期9卷 66-76页

作者： Rong-Chang ZENG KeJIANG Shuo-Qi li Fen Zhang Hong-Zhi CUI En-hou HAN College of Materials Science and Engineering Shandong University of Science and Technology Qingdao 266590 China State Key Laboratory of Mining Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology Shandong University of Science and Technology Qingdao 266590 China Institute of Metals Research Chinese Academy of Sciences Shenyang 110016 China

Preparation of titanium film on magnesium substrate faces a challenge due to non-Fickian inter-diffusion between titanium and magnesium. Aluminum can build a bridge between titanium and magnesium. Al/Ti duplex coatings were deposited on magnesium alloy AZ31B using magnetron sputtering （MS）. The low temperature diffusion bonding behavior of the MglAl/Ti coating was investigated through SEM and its affiliated EDS. The phase structure and critical load of the coatings were examined by means of XRD and scratch tests, respectively, The results demonstrated that the bonding strength was significantly improved after a post heat treatment （HT） at a temperature of 210℃. The diffusion mechanism of the interfaces of MglAI and Al/Ti in the coating was discussed based on the analysis of formation energy of vacancies and diffusion rates. The Al/Ti dual layer enhanced the corrosion resistance of the alloy. And the HT process further increased the corrosion resistance of the coated alloy. This result implies that a post HTat a lower temperature after MS is an effective approach to enhance the bonding strength and corrosion resistance of the Al/Ti film on Mg alloys.

关键词： magnesium alloy aluminum/titanium coating magnetron sputtering (MS) diffusion bonding strength

Spatio-temporal analysis of the incidence of colorectal cancer in Guangzhou,2010-2014

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Chinese Journal of Cancer 2017年第10期36卷 516-523页

作者： Ke li Guo-Zhen lin Yan li hang Dong Huan Xu Shao-Fang Song Ying-Ru liang Hua-Zhang liu Department of Biostatistics and Cancer Registration Guangzhou Center for Disease Control and PreventionGuangzhou 510440GuangdongP.R.China

Introduction: Colorectal cancer(CRC) is a common type of neoplasm. This study examined the spatio?temporal distribution of the CRC incidence in Guangzhou during 2010–***: Colorectal cancer incidence data were obtained from the Guangzhou Cancer Registry System. Spatial autocorrelation analysis and a retrospective spatio?temporal scan were used to assess the spatio?temporal cluster distribution of CRC ***: A total of 14,618 CRC cases were registered in Guangzhou during 2010–2014, with a crude incidence of 35.56/100,000 and an age?standardized rate of incidence by the world standard population(ASRIW) of 23.58/100,000. The crude incidence increased by 19.70% from 2010(32.88/100,000) to 2014(39.36/100,000) with an average annual percentage change(AAPC) of 4.33%. The AAPC of ASRIW was not statistically significant. The spatial autocorrelation analysis revealed a CRC incidence hot spot in central urban areas in Guangzhou City, which included 25 streets in southwestern Baiyun District, northwestern Haizhu District, and the border region between liwan and Yuexiu Dis?tricts. Three high? and five low?incidence clusters were identified according to spatio?temporal scan of CRC incidence clusters. The high?incidence clusters were located in central urban areas including the border regions between Bai?yun, Haizhu, liwan, and Yuexiu ***: This study revealed the spatio?temporal cluster pattern of the incidence of CRC in Guangzhou. This information can inform allocation of health resources for CRC screening.

关键词： Colorectal cancer Spatial analysis Spatial autocorrelation Spatio-temporal clustering