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bone Research 2023年第2期11卷 368-383页

作者： Yu Sheng li Xiao Wang bo Hu Qi Sun Mei Wan Andrew Carr Shen liu Xu Cao Department of Orthopedic Surgery The Johns Hopkins University School of MedicineBaltimoreMD21205USA Nuffield Department of Orthopedics Rheumatology and Musculoskeletal SciencesBotnar Research CentreUniversity of OxfordWindmill RoadOxford OX 37 LDUK

PPeritendinous adhesion formation(PAF)can substantially limit the range of motion of ***,the origin of myofibroblasts in PAF tissues is still *** this study,we found that the concentration of active TGF-β1 and the numbers of macrophages,mesenchymal stromal cells(MSCs),and myofibroblasts in human and mouse adhesion tissues were ***,knockout of TGF-β1 in macrophages or TGF-β1R2 in MSCs inhibited PAF by reducing MSC and myofibroblast infiltration and collagenⅠandⅢdeposition,***,we found that MSCs differentiated into myofibroblasts to form adhesion *** injection of the TGF-β–neutralizing antibody 1D11 during the granulation formation stage of PAF significantly reduced the infiltration of MSCs and myofibroblasts and,subsequently,*** results suggest that macrophage-derived TGF-β1 recruits MSCs to form myofibroblasts in peritendinous *** improved understanding of PAF mechanisms could help identify a potential therapeutic strategy.

关键词： inhibited injection MSC

Effect of tankyrase 1 on autophagy in the corpus cavernosum smooth muscle cells from ageing rats with erectile dysfunction and its potential mechanism

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Asian Journal of Andrology 2010年第5期12卷 744-752页

作者： Jian Zhang Xiao-Jun Wu De-Xiang Zhuo Tao liu Wei-Ren li Ze-Bin mao Zhong-Cheng Xin Andrology Center First Hospital Peking University Beijing 100009 China Institute of Urinary Surgery Southwest Hospital Third Military Medical University Chongqing 400038 China The Department of Biochemistry and Molecular Biology Health Science Center Peking University Beijing 100191 China

This study compared tankyrase 1 expression and autophagy quantity between erectile dysfunction （ED） and non-ED rats＇ corpus cavernosum smooth muscle cells （CSMCs）. This study aslo explored the effect and possible mechanism of tankyrase 1 on autophagy and cell proliferation in ageing ED rats＇ CSMCs. The intracavernous pres- sure and mean systemic arterial pressure were measured to investigate erectile function so that eight 24-month-old ED and eight 8-month-old male Wistar rats were choosed respectively. The rat CSMCs were isolated and cultured by enzyme digestion, in which tankyrase 1 expression and autophagy quantity were compared. Tankyrase 1 over-expression was induced with plasmid transfection by lipofectamine^TM. The effect of tankyrase 1 overexpression on proliferation, autophagy and mTOR pathway in 24-month-old ED rats＇ CSMCs was measured by the cell growth curve in MTT assay, cell cycle analysis in flow cytometry （FCM）, key protein expression in Western blot, autophagy quantity in transmission electron microscopy, monodansylcadaverine staining and GFP-LC3 fluorescence. The primary CSMCs were confirmed by immunofluorescence, and the purity was 99.1% in FCM. Compared with that of 8-month-old rats, tankyrase 1 expression and autophagy quantity significantly decreased in 24-month-old ED rats＇ primary CSMCs （P 〈 0.01）. Tankyrase 1 overexpression significantly increased the growth rate （P 〈 0.05） and increased the S phase of cell cycle （P 〈 0.01）. The autophagosome quantity was remarkably increased （P 〈 0.01）, LC3-Ⅰ/Ⅱ and Beclin 1 were upregulated （P 〈 0.01 and P 〈 0.05）, and p-p70S6K （Thr389） was downregulated in 24-month-old ED rat CSMCs （P 〈 0.05）. In conclusion, Tankyrase 1 and autophagy decrease in the CSMCs from aging rats with ED, and tankyrase 1 may have a positive effect on proliferation by enhancing autophagy and regulating the mTOR signalling pathway.

关键词： ageing autophagy corpora cavernosum corpus cavemosum smooth muscle cells erectile dysfunction senile tankyrase

Isolation and Functional Characterisation of the Genes Encoding Δ~8-Sphingolipid Desaturase from Brassica rapa

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Journal of Genetics and Genomics 2012年第1期39卷 47-59页

作者： Shu-Fen li li-Ying Song Wei-bo Yin yu-Hong Chen liang Chen Ji-lin li Richard R.-C.Wang Zan-Min Hu Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China Graduate University of Chinese Academy of Sciences Beijing 100049China Biological College Harbin Normal UniversityHarbin 150080China USDA-ARS FRRLUtah State UniversityLoganUT 84322-6300USA

8-Sphingolipid desaturase is the key enzyme that catalyses desaturation at the C8 position of the long-chain base of sphingolipids in higher plants. There have been no previous studies on the genes encoding AS-sphingolipid desaturases in Brassica rapa. In this study, four genes encoding AS-sphingolipid desaturases from B. rapa were isolated and characterised. Phylogenetic analyses indicated that these genes could be divided into two groups： BrD8A, BrD8C and BrD8D in group I, and BrD8B in group II. The two groups of genes diverged before the separation of Arabidopsis and Brassica. Though the four genes shared a high sequence similarity, and their coding desaturases all located in endoplasmic reticulum, they exhibited distinct expression patterns. Heterologous expression in Saccharomyces cerevisiae revealed that BrD8A/B/C/D were functionally diverse AS-sphingolipid desaturases that catalyse different ratios of the two products 8（Z）- and 8（E）-C18-phytosphingenine. The aluminium tolerance of transgenic yeasts expressing BrD8A/B/C/D was enhanced compared with that of control cells. Expression of BrD8A in Arabidopsis changed the ratio of 8（Z）：8（E）-C 18-phytosphingenine in transgenic plants. The information reported here provides new insights into the biochemical functional diversity and evolutionary relationship of AS-sphingolipid desaturase in plants and lays a foundation for further investigation of the mechanism of 8（Z）- and 8（E）-C18- phytosphingenine biosynthesis.

关键词： △8-Sphingolipid desaturase Biochemical functional diversity Brassica rapa Expression pattern Long-chain base (LCB)

Effect of Nitrogen Regimes on Grain Yield,Nitrogen Utilization,Radiation Use Efficiency,and Sheath Blight Disease Intensity in Super Hybrid Rice

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Journal of Integrative Agriculture 2012年第1期11卷 134-143页

作者： li Di-qin TANG Qi-yuan ZHANG Yun-bo QIN Jian-quan li Hu CHEN li-jun YANG Sheng-hai ZOU Ying-bin PENG Shao-bing State Key Laboratory of Hybrid Rice Hunan Agricultural University Changsha Hunan 410128 P.R.China Crop Physiology and Production Center （CPPC） Huazhong Agricultural University Wuhan 430070 P..R.China

Poor nitrogen use efficiency in rice production is a critical issue in China. Site-specific N managements （SSNM） such as real-time N management （RTNM） and fixed-time adjustable-dose N management （FTNM） improve fertilizer-N use efficiency of irrigated rice. This study was aimed to compare the different nitrogen （N） rates and application methods （FFP, SSNM, and RTNM methods） under with- and without-fungicide application conditions on grain yield, yield components, solar radiation use efficiency （RUE）, agronomic-nitrogen use efficiency （AEN）, and sheath blight disease intensity. Field experiments were carried out at liuyang County, Hunan Province, China, during 2006 and 2007. A super hybrid rice liangyou 293 （LY293） was used as experimental material. The results showed that RTNM and SSNM have great potential for improving agronomic-nitrogen use efficiency without sacrificing the grain yield. There were significant differences in light interception rate, sheath blight disease incidence （DI） and the disease index （ShBI）, and total dry matter among the different nitrogen management methods. The radiation use efficiency was increased in a certain level of applied N. But, the harvest index （HI） decreased with the increase in applied N. There is a quadratic curve relationship between grain yield and applied N rates. With the same N fertilizer rate, different fertilizer-N application methods affected the RUE and grain yield. The fungicide application not only improved the canopy light interception rate, RUE, grain filling, and harvest index, but also reduced the degree of sheath blight disease. The treatment of RTNM under the SPAD threshold value 40 obtained the highest yield. While the treatment of SSNM led to the highest nitrogen agronomic efficiency and higher rice yield, and decreased the infestation of sheath blight disease dramatically as well. Nitrogen application regimes and diseases control in rice caused obvious effects on light interception rate, RUE, and HI. Optim

关键词： super hybrid rice real-time N management fixed-time adjustable-does N management grain yield sheath blight radiation use efficiency agronomic-nitrogen use efficiency

Formation of novel TRIP-ductilized bulk metallic glass composites by manipulating nucleation kinetics via minor alloying

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Rare Metals 2023年第7期42卷 2182-2188页

作者： Xi-Lai Jiang Wen-li Song Ming Yang Yuan-bo Zhou Yan Huang Zhi-Chao Lu Yi-bo Zhang Jie Dong liang Wang bo Peng Chun-Ming Hu Jin-Kui Zhao Sheng-Zhong Kou Dong Ma School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou730050China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing100049China Spallation Neutron Source Science Center Dongguan523803China Neutron Science Platform Songshan Lake Materials LaboratoryDongguan523808China State Key Laboratory for Advanced Metals and Materials University of Science and Technology BeijingBeijing100083China Institute of Physics Chinese Academy of Sciences(CAS)Beijing100190China

A series of conical bulk metallic glass composites(BMGCs)with compositions of Zr_(47)Cu_(47-x)Al_(5)Ag_(1)Ga_(x)(x=0,1,2,4;at%)were synthesized using the minor alloying method to investigate the effect of Ga addition ... 详细信息

关键词： alloying properties glass

Change of BMD after Weaning or Resumption of Menstruation in Chinese Women with Different FokI VDR-genotypes:A Randomized,Placebo-controlled,Calcium Supplementation Trial

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Biomedical and Environmental Sciences 2011年第3期24卷 243-248页

作者： YU bo WU HongYing li Fang GONG Jie ZHOU DunJin ZHANG ZhiFeng YANG XiaoGuang HUANG ZhenWu National Institute for Nutrition and Food Safety Chinese Center for Disease Control and Prevention Beo'ing 100021 China Department of Radiography Union Hospital Huazhong University of Science & Technology Wuhan 430022 Hubei China Wuhan Regional Center for Disease Control and Prevention Wuhan 430024 Hubei China

Objective To investigate the effect of calcium supplementation on bone mineral density （BMD） in Chinese women with different Fokl vitamin D receptor （VDR） genotypes （FF, Ff, and ff） after weaning or resumption of menstruation during lactation. Methods A total of 40 subjects with the same Fokl VDR genotype were randomly divided into two groups： one received calcium tablet （600 mg once daily as CaCO3） and the other placebo tablet once daily for 1 year. At baseline, BMD was measured by dual-energy X-ray absorptiometry at lumbar spine （L2-L4） and at left hip whereas serum PICP, serum OC, and urinary CTX, serum 25（OH）VitD3, and serum estradiol were measured at weaning and I year thereafter. Results After the intervention, BMD at lumbar spine and at left hip increased significantly in all these women with a trend among different Fokl VDR genotypes such as FF 〉 Ff 〉 ff （P〈O.05, 〈0.01, and 〈0.001, respectively）. BMD at lumbar spine in women with FF VDR genotype increased much more rapidly than in those with ff VDR genotype （P〈0.05）. Compared with the control group women with the FF genotype regained more BMD after calcium supplementation （P〈0.05）. Conclusion Daily calcium 600 mg supplementation has beneficial effect on the bone health of women with FF VDR genotype.

关键词： Fokl VDR genotype Calcium supplementation bone mineral accretion after weaning Chinese women Low dietary calcium intake.

ASSESSMENT OF WATER RESOURCES AT HONGHE NATIONAL NATURE RESERVE

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湿地科学 2004年第2期2卷 145-152页

作者： liU Zheng-mao LU Xian-Guo ZHAO Chun-Hui ZHAO Yan-bo QI Han-Qiang CollegeofMunicipalandEnvironmentalEngineering HarbinInstituteofTechnologyHarbinHeilongjiang150090China NortheastInstituteofGeographyandAgriculturalEcology CASChangchunJilin130012China HeilongjiangAgriculturalReclamationSurvey DesignandResearchInstituteJiamusiHeilongjiang154002China

A detailed assessment on water resources of HNNR is to find the changing rules in time and space scale of water resources of HNNR and its adjacent areas, and the generating and degrading factors of wetland and provide scientific base on restoring and managing the hydrologic regime for planning and designing at HNNR. both the assessment area and its adjacent watershed of Bielahong River belong to the same region in the climate and surface features. Total of 46 years of serial data from 1956 2001 in the Bielahong Hydrology Station was employed. Typical analysis of the serial runoff was conducted by adopting the residual mass curve method. The calculation methods of hydrological parameters are valuable for analyzing the water balance of HNNR. The results showed that the inputs of 118.29×10 6 m 3 of the surface runoff and 1 478km 2 of the areas of natural watershed in HNNR were decreased. At the same time some measurements to control and prevent water resources decreased have been proposed.

关键词：洪河生态系统水资源生态环境环境保护

有机污染物和碳纳米颗粒:吸附机理及影响因素(英文)

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2014年第8期15卷 606-617页

作者： Hong-bo PENG Di ZHANG Hao li Chi WANG bo PAN Faculty of Environmental Science & Engineering Kunming University of Science & Technology

研究目的:碳纳米颗粒由于其独特的优良性能被广泛应用,从而不可避免地进入环境。进入环境的碳纳米颗粒与有机污染物有很强的相互作用,并且碳纳米颗粒对有机污染物的吸附可能会影响污染物的环境风险和迁移。因此,本研究总结了目前有机污... 详细信息

研究目的:碳纳米颗粒由于其独特的优良性能被广泛应用,从而不可避免地进入环境。进入环境的碳纳米颗粒与有机污染物有很强的相互作用,并且碳纳米颗粒对有机污染物的吸附可能会影响污染物的环境风险和迁移。因此,本研究总结了目前有机污染物在碳纳米颗粒上吸附的研究进展,为有机污染物及碳纳米颗粒的环境行为研究及风险评价提供重要信息。重要结论:有机污染物和碳纳米颗粒的主要相互作用有憎水性、静电、氢键和π-π作用,这些作用及它们的强度受碳纳米颗粒的表面性质、形态及有机污染物的分子大小、结构和官能团等影响。不同的吸附机理可能同时控制有机物在碳纳米颗粒上的吸附过程,而且在不同的环境条件下吸附控制机理不同。区分吸附控制机理和影响因素的研究对预测有机污染物和碳纳米颗粒的环境行为和风险非常重要。

关键词：有机污染物环境行为碳纳米颗粒相互作用机理

Effect of spin-reorientation transition of cell boundary phases on the temperature dependence of magnetization and coercivity in Sm_(2)Co_(17) magnets

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Chinese Physics B 2023年第12期32卷 541-546页

作者：涂思思刘雷周波董创辉叶力铭孙颖莉丁勇闫阿儒毛信表 College of Chemical Engineering Zhejiang University of TechnologyHangzhou 310014China CISRI and NIMTE Joint Innovation Center for Rare Earth Permanent Magnets CAS Key Laboratory of Magnetic Materials and DevicesNingbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China

Four Sm_(2)Co_(17)magnets with spin-reorientation transition(SRT)of cell boundary phases(CBPs)are prepared by liquid-phase *** temperature of the SRT of CBPs(T_(SR)^(1:5))is regulated from 125 K to 195 K by adding 0 wt.%,3 wt.%,6 wt.%and 9 wt.%Dy_(88)Cu_(12)alloy *** effect of SRT of Sm_(2)Co_(17)magnet CBPs on the temperature dependence of the magnetization(M-T)and coercivity(H-T)is systematically *** temperature dependence of the magnetization is influenced by the SRT of *** M-T curves measured during the heating process are larger than those measured during the cooling process when TT_(SR)^(1:5)the M-T curves overlap and the M-T derivation curve shows that the magnetization of the magnet has low temperature dependence of magnetization above T_(SR)^(1:5).With increasing T_(SR)^(1:5),the initial temperature of the low temperature dependence of magnetization shifts towards a higher *** coercivity temperature coefficient becomes positive as the SRT effect increases,and the temperature range of the positive coercivity temperature coefficient moves towards higher temperatures as T_(SR)^(1:5)*** reveals that SRT of CBPs has little effect on the temperature dependence of magnetization above T_(SR)^(1:5),while the temperature dependence of coercivity is *** temperature range of magnetization and coercivity with low temperature dependence tends towards higher temperatures,which is conducive to the preparation of magnets with a low temperature coefficient at higher temperatures.

关键词： spin-reorientation transformation low-temperature coefficient Sm_(2)Co_(17)magnets

A Dietary Supplement Jinghuosu Ameliorates Reproductive Damage Induced by Tripterygium Glycosides

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Chinese Journal of Integrative Medicine 2024年第4期30卷 330-338页

作者： MA Jing SUN bo TE li-ger HUANG Xin ZUO Xin HAN Xiao-ke WANG Shu-song Hebei Key Laboratory of Reproductive Medicine Hebei Institute of Reproductive HealthHebei Reproductive Health HospitalShijiazhuang(050071)China Graduate School of Hebei Medical University Shijiazhuang(050017)China School of Chemistry and Materials Science Hebei Normal UniversityShijiazhuang(050024)China Xingtai Infertility Specialist Hospital XingtaiHebei Province(054000)China

Objective To determine the possible protective effects of Jinghuosu,a dietary supplement(DS),on tripterygium glycosides(TG)-induced reproductive system injury in rats and its underlying *** A reproductive damage model was established in rats by feeding of ***-eight male Sprague Dawley rats were randomly divided into 4 groups using a random number table(n=7 in each):control(C)group,model(M)group,DS group and L-carnitine(LC)*** in M,DS and LC groups received 40 mg/kg TGs *** from the 5th week,after administration of TGs for 4 h every day,rats in DS and LC groups were administered with 2.7 g/kg DS and 0.21 g/kg LC,respectively,for protective treatment over the next 4 *** in Group C continued to receive the control ***-eosin staining was used for histopathological analysis of rat testicular ***-linked immunosorbent assay was performed to measure alkaline phosphatase(ALP),lactate dehydrogenase,alcohol dehydrogenase,total antioxidant capacity(T-AOC),superoxide dismutase,glutathione peroxidase(GSH-Px),and malondialdehyde(MDA)*** assay was used to determine the serum testosterone *** real-time PCR and Western blotting were conducted to analyze the expression of genes and proteins related to the testosterone synthesis pathway and the nuclear factor erythroid 2-related factor 2/heme oxygenase 1 antioxidant *** Oral administration of TGs induced significant increases in the testicular levels of zinc transporter 1 and MDA(Plity,and serum testosterone,serum zinc,testicular zinc,Zrt-,Irt-like protein 1,ALP,luteinizing hormone(LH)receptor,steroidogenic acute regulatory protein,Cytochrome P450 family 11 subfamily A member 1,3β-hydroxysteroid dehydrogenase 1 T-AOC,GSH-Px,nuclear factor erythroid 2-related factor 2,heme oxygenase-1 and NAD(P)H:quinone oxidoreductase 1 levels decreased following TG

关键词： dietary supplement Jinghuosu tripterygium glycosides reproductive damage infertility