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Flavonoid-Rich Extract of Oldenlandia diffusa(Willd.)*** Gastric Cancer by Activation of Caspase-Dependent Mitochondrial Apoptosis

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Chinese Journal of Integrative Medicine 2023年第3期29卷 213-223页

作者： liNG Jia-yin WANG Qiu-lan liANG Hao-nan liU Qing-bo YIN Dong-hong liN li First School of Clinical Medicine Gansu University of Chinese MedicineLanzhou(730000)China Gansu Provincial Hospital Lanzhou(730000)China College of Pharmacy Gansu University of Chinese MedicineLanzhou(730000)China Institute of Chinese(Tibetan)Medicine Resources Gansu University of Chinese MedicineLanzhou(730000)China

Objective:To evaluate the apoptosis and cycle arrest effects of Oldenlandia diffusa flavonoids on human gastric cancer cells,determine the action mechanisms in association with the mitochondrial dependent signal transduction pathway that controls production of reactive oxygen species(ROS),and evaluate the pharmacodynamics of a mouse xenotransplantation model to provide a reference for the use of flavonoids in prevention and treatment of gastric ***:Flavonoids were extracted by an enzymatic-ultrasonic assisted method and purified with D-101 *** components were characterized by high-performance liquid *** lines MKN-45,AGS,and GES-1 were treated with different concentrations of flavonoids(64,96,128,and 160μg/mL).The effect of flavonoids on cell viability was evaluated by MTT method,and cell nuclear morphology was observed by Hoechst *** apoptosis rate and cell cycle phases were measured by flow cytometry,the production of ROS was detected by laser confocal microscope,the mitochondrial membrane potential(MMP)were observed by fluorescence microscope,and the expression of apoptotic proteins related to activation of mitochondrial pathway were measured by ***-45cells were transplanted into BALB/c nude mice to establish a xenograft tumor *** and eosin staining was used to reveal the subcutaneous tumor *** tumor volume and tumor weight were measured,the expression levels of proliferation markers proliferating cell nuclear antigen(PCNA)and Ki-67were detected by immunohistochemistry,and the expression levels of CA72-4 were measured by enzyme linked immunosorbent ***:Oldenlandia diffusa flavonoids inhibited proliferation of MKN-45 and AGS human gastric cancer cells,arrested the cell cycle in G1/S phase,induced accumulation of ROS in the process of apoptosis,and altered *** addition,flavonoids increased Apaf-1,Cleaved-Caspase-3,and Bax,and decreased Cyclin A,Cdk2,Bcl-2,Pro-Caspase-9,a

关键词： Chinese medicine Oldenlandia diffusa flavonoids gastric cancer apoptosis mitochondria

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Increased CXCL13 and CXCR5 in Anterior Cingulate Cortex Contributes to Neuropathic Pain-Related Conditioned Place Aversion

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Neuroscience Bulletin 2019年第4期35卷 613-623页

作者： Xiao-bo Wu li-Na He Bao-Chun Jiang Xue Wang Ying Lu Yong-Jing Gao Institute of Pain Medicine Institute of Special Environmental Medicine Nantong University Nantong 226019 China Department of Nutrition and Food Hygiene School of Public Health Nantong University Nantong 226019 China Co-innovation Center of Neuroregeneration Nantong University Nantong 226001 China

Pain consists of sensory-discriminative and emotional-affective *** anterior cingulate cortex(ACC)is a critical brain area in mediating the affective ***,the molecular mechanisms involved remain largely *** recent study indicated that C-X-C motif chemokine 13(CXCL13)and its sole receptor CXCR5 are involved in sensory sensitization in the spinal cord after spinal nerve ligation(SNL).Whether CXCL13/CXCR5 signaling in the ACC contributes to the pathogenesis of pain-related aversion remains ***,we showed that SNL increased the CXCL13 level and CXCR5 expression in the ACC after *** of CXCR5 by microinjection of Cxcr5 shRNA into the ACC did not affect SNL-induced mechanical allodynia but effectively alleviated neuropathic painrelated place avoidance ***,electrophysiological recording from layer Ⅱ-Ⅲ neurons in the ACC showed that SNL increased the frequency and amplitude of spontaneous excitatory postsynaptic currents(sEPSCs),decreased the EPSC paired-pulse ratio,and increased the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor/N-methyl-D-aspartate receptor ratio,indicating enhanced glutamatergic synaptic ***,superfusion of CXCL13 onto ACC slices increased the frequency and amplitude of spontaneous ***-injection of Cxcr5 shRNA into the ACC reduced the increase in glutamatergic synaptic transmis sion induced by ***,these results suggest that CXCL13/CXCR5 signaling in the ACC is involved in neuropathic pain-related aversion via synaptic potentiation.

关键词： CXCL13 CXCR5 Anterior cingulate cortex Neuropathic pain Conditioned place aversion Synaptic transmission

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Perfect invisibility concentrator with simplified material parameters

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Frontiers of physics 2018年第5期13卷 3-8页

作者： Meng-Yin Zhou lin Xu Lu-Chan Zhang Jiang Wu Yan-bo li Huan-Yang Chen Institute of Electromagnetics and Acoustics and Department of Electronic Science Xiamen University Xiamen 361005 China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of China Chengdu 610054 China

We present a series of invisibility concentrators with simplified material parameters beyond trans- formation optics. One of them can achieve the perfect invisible effect at frequencies of Fabry-Perot resonances, while others have very small scattering. The required materials are feasible in practice. Analytical calculations and numerical simulations confirm the functionalities of these devices.

关键词： perfect-invisibility concentrator simplified material parameters Fabry-Perot resonances scattering cross section

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A large-scale experimental simulator for natural gas hydrate recovery and its experimental applications

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Petroleum 2023年第4期9卷 607-612页

作者： Yang Ge Qingping li Xin Lv Mingqiang Chen bo Yang Benjian Song Jiafei Zhao Yongchen Song State Key Laboratory of Natural Gas Hydrates Beijing100028China Key Laboratory of Ocean Energy Utilization and Energy Conservation of Ministry of Education Dalian University of TechnologyDalian116024China China National Offshore Oil Corporation Research Institute Co.Ltd Beijing100028China

To facilitate the recovery of natural gas hydrate(NGH)deposits in the South China Sea,we have designed and developed the world's largest publicly reported experimental simulator for NGH *** system can also be used to perform CO_(2) capture and sequestration experiments and to simulate NGH recovery using CH_(4)/CO_(2) *** system was used to prepare a shallow gas and hydrate reservoir,to simulate NGH recovery via depressurization with a horizontal well.A set of experimental procedures and data analysis methods were prepared for this *** analyzing the measurements taken by each probe,we determined the temperature,pressure,and acoustic parameter trends that accompany NGH *** results demonstrate that the temperature fields,pressure fields,acoustic characteristics,and electrical impedances of an NGH recovery experiment can be precisely monitored in real time using the aforementioned experimental ***,fluid production rates can be calculated at a high level of *** was concluded that(1)the optimal production pressure differential ranges from 0.8 to 1.0 MPa,and the wellbore will clog if the pressure differential reaches 1.2 MPa;and(2)during NGH decomposition,strong heterogeneities will arise in the surrounding temperature and pressure fields,which will affect the shallow gas stratum.

关键词： Natural gas hydrate(NGH) Experimental NGH recovery simulator Recovery by depressurization Wellbore clogging Shallow gas

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Sonic hedgehog elevates N-myc gene expression in neural stem cells

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Neural Regeneration Research 2012年第22期7卷 1703-1708页

作者： Dongsheng liu Shouyu Wang Yan Cui Lun Shen Yanping Du Guilin li bo Zhang Renzhi Wang Department of Neurosurgery First Affiliated Hospital Dalian Medical University Dalian 116011 Liaoning Province China Chinese Academy of Medical Sciences Department of Neurosurgery Peking Union Medical College Hospital Beijing 100730 China

Proliferation of neural stem cells is regulated by the secreted signaling molecule sonic hedgehog. In this study, neural stem cells were infected with recombinant adeno-associated virus expressing sonic hedgehog-N-enhanced green fluorescent protein. The results showed that overexpression of sonic hedgehog in neural stem cells induced the increased expression of Gill and N-myc, a target gene of sonic hedgehog. These findings suggest that N-myc is a direct downstream target of the sonic hedgehog signal pathway in neural stem cells. Sonic hedgehog and N-myc are important mediators of sonic hedgehog-induced proliferation of neural stem cells.

关键词： stem cells neural stem cells sonic hedgehog signal pathway N-myc gene proliferation targetgene neural regeneration

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Effect of TiO_2 on Crystallization,Microstructure and Mechanical Properties of Glass-ceramics

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Journal of Iron and Steel Research International 2015年第12期22卷 1113-1117页

作者： Jian YANG Shen-gen ZHANG bo liU De-an PAN Chun-li WU Alex A.VOliNSKY Institute for Advanced Materials and Technology University of Science and Technology Beijing Department of Mechanical Engineering University of South Florida

The effect of TiO2addition to the stainless steel slag glass-ceramics was studied. Different mass percenta- ges of TiO2 were added to four samples of the parent glass made from stainless steel slag and cullet. The tempera- tures of nucleation and crystallization were determined by differential scanning calorimetry （DSC）. According to X- ray diffraction （XRD） and scanning electron microscopy （SEM） analysis, adding TiO2 refined grains and restricted the formation of the akermanite phase by capturing Ca2＋ to form the perovskite phase. Diopside was the main crystal phase of the glass-ceramics. The bending strength and Vickers hardness increased with the addition of TiO2. The optimal amount of TiO2 was 7 mass%, and the highest bending strength and Vickers hardness were 144.6 MPa and 6.26 GPa, respectively.

关键词： glass-ceramics TiO2 crystallization mechanical property

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Multi-twinned gold nanoparticles with tensile surface steps for efficient electrocatalytic CO_(2) reduction

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Science China Chemistry 2022年第11期65卷 2188-2196页

作者： li-Wei Chen Yu-Chen Hao Jiani li linyu Hu Yu Guo Shuai li Di liu Zhejiaji Zhu Si-Qian Wu Hui-Zi Huang An-Xiang Yin bo Wang Ya-Wen Zhang Ministry of Education Key Laboratory of Cluster Science Beijing Key Laboratory of Photoelectronic/Electrophotonic Conversion MaterialsAdvanced Technology Research Institute(Jinan)School of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 100081China Beijing National Laboratory for Molecular Sciences State Key Laboratory of Rare Earth Materials Chemistry and ApplicationsPKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic ChemistryCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China

CO_(2) reduction reactions(CO_(2)RR) powered by renewable electricity can directly convert CO_(2) to hydrocarbons and fix the intermittent sustainable energy in portable chemical fuels. It is of great importance to develop advanced catalysts that can boost CO_(2)RR with high activity, selectivity, and efficiency at low overpotentials. Here, we report the solution synthesis using H_(2)O_(2) to modify the surface structures of gold multi-twinned nanoparticles(AuMPs) and create tensile surface steps. Calculations predicted significantly enhanced CO_(2) adsorption and boosted CO_(2)RR capabilities with inhibited hydrogen evolution reaction activity for the tensile surface steps with modified electronic structure. The H_(2)O_(2)-treated AuMPs with surface steps and 3.83% tensile lattices showed much higher activity and selectivity at lower overpotentials for CO_(2)RR than pristine gold *** CO-production current density reached about 98 mA cm^(-2) with a Faradaic efficiency of 95.7% at -0.30 V versus reversible hydrogen electrode in the flow cell, showing a half-cell energy efficiency as high as ~83%. Our strategy represents a rational catalyst design by engineering the surface structures of metal nanoparticles and may find more applicability in future electrocatalysis.

关键词： gold nanoparticles tensile lattice surface steps electrocatalysis CO_(2)reduction

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Atmospheric Parameters and Kinematic Information for the M Giant Stars from LAMOST DR9

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Research in Astronomy and Astrophysics 2023年第5期23卷 78-90页

作者： Dan Qiu Hao Tian Jing li Chao liu lin Long Jian-Rong Shi Ming Yang bo Zhang Key Laboratory of Space Astronomy and Technology National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences 100049China Institute for Frontiers in Astronomy and Astrophysics Beijing Normal UniversityBeijing 102206China School of Physics and Astronomy China West Normal UniversityNanchong 637002China Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of SciencesBeijing 100101China

A catalog of more than 43,000 M giant stars has been selected by li et *** the ninth data release of *** the data-driven method SLAM,we obtain the stellar parameters(T_(eff),logg,[M/H],[α/M])for all the M giant stars with uncertainties of 57 K,0.25 dex,0.16 dex and 0.06 dex at SNR>100,*** those stellar parameters,we constrain the absolute magnitude in the K-band,which brings distance with relative uncertainties around 25%*** velocities are also calculated by applying cross correlation on the spectra between 8000 and 8950?with synthetic spectra from ATLAS9,which covers the CaⅡ*** between our radial velocities and those from APOGEE DR17 and Gaia DR3 shows that our radial velocities have a system offset and dispersion around 1 and 4.6 km s^(-1),*** the distances and radial velocities combining with the astrometric data from Gaia DR3,we calculate the full 6D position and velocity information,which are able to be used for further chemo-dynamic studies on the disk and substructures in the halo,especially the Sagittarius Stream.

关键词： methods data analysis-stars atmospheres-Galaxy disk-stars abundances

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Impact of HBV replication in peripheral blood mononuclear cell on HBV intrauterine transmission

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Frontiers of Medicine 2017年第4期11卷 548-553页

作者： Xiaohong Shi Xuefei Wang Xixi Xu Yongliang Feng Shuzhen li Shuying Feng bo Wang Suping Wang Department of Epidemiology School of Public Health Shanxi Medical University Taiyuan 030001 China Department of Obstetrics and Gynaecology The Third PeopIeHospital of Taiyuan City Taiyuan 030001 China

This study determined the effect of hepatitis B virus （HBV） replication in peripheral blood mononuclear cell （PBMC） from HBsAg-positive mothers on HBV intrauterine transmission. A total of 150 HBsAg- positive mothers and their neonates were recruited in this study. Within 24 h after birth, HBV serological markers, serum HBV DNA, PBMC HBV relaxed circular DNA （rcDNA）, and covalently closed circular DNA （cccDNA） were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis. The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chi- square test and logistic regression. The rate of HBV intrauterine transmission was 8.00% （12/150） in the 150 neonates born to HBsAg-positive mothers. The positivities of PBMC HBV rcDNA and cceDNA in the HBsAg- positive mothers were 36.67% （55/150） and 10% （15/150）, respectively. Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission （OR = 6.003, 95% Ch 1.249-28.855）. Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA （OR = 3.896, 95% CI： 1.929-7.876） and PBMC HBV cccDNA （OR = 3.74, 95% CI： 1.186--11.793） in the HBsAg-positive mothers. Administration of hepatitis B immune globulin was a protective factor ofPBMC HBV cccDNA （OR = 0.312, 95% CI： 0.102-0.954） during pregnancy. The positivity ofPBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers （Zz= 5.087, P = 0.024）. This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.

关键词： PBMC HBV cccDNA HBV rcDNA HBV intrauterine transmission

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Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Enhance the Osteoblastic Differentiation of Periodontal ligament Stem Cells Under High Glucose Conditions Through the PI3K/AKT Signaling Pathway

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Biomedical and Environmental Sciences 2022年第9期35卷 811-820页

作者： YANG Shuo ZHU Biao TIAN Xiao Yu YU Han Ying QIAO bo ZHAO li Sheng ZHANG Bin Department of Stomatology The First Medical CenterChinese PLA General HospitalBeijing 100853China Department of Stomatology Fuxing HospitalCapital Medical UniversityBeijing 100038China

Objective High glucose(HG)can influence the osteogenic differentiation ability of periodontal ligament stem cells(PDLSCs).Human umbilical cord mesenchymal stem cell-derived exosomes(hUCMSC-exo)have broad application prospects in tissue *** current study aimed to explore whether hUCMSC-exo could promote the osteogenic differentiation of hPDLSCs under HG conditions and the underlying *** We used a 30 mmol/L glucose concentration to simulate HG ***-8 assay was performed to evaluate the effect of hUCMSC-exo on the proliferation of *** phosphatase(ALP)staining,ALP activity,and qRT-PCR were performed to evaluate the pro-osteogenic effect of hUCMSC-exo on *** blot analysis was conducted to evaluate the underlying *** The results of the CCK-8 assay,ALP staining,ALP activity,and qRT-PCR assay showed that hUCMSC-exo significantly promoted cell proliferation and osteogenic differentiation in a dosedependent *** Western blot results revealed that hUCMSC-exo significantly increased the levels of p-PI3K and p-AKT in cells,and the effect was inhibited by LY294002(PI3K inhibitor)or MK2206(AKT inhibitor),***,the increases in osteogenic indicators induced by hUCMSC-exo were significantly suppressed by LY294002 and *** hUCMSC-exo promote the osteogenic differentiation of hPDLSCs under HG conditions through the PI3K/AKT signaling pathway.

关键词： Exosomes Human umbilical cord mesenchymal stem cell Periodontal ligament stem cell Osteogenic differentiation High glucose PI3K/AKT

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